The characterization and correlation between the phenotypic and genotypic resistance of < em > Campylobacter < /em > spp < em > . < /em > isolates from commercial broilers and native chickens in the south of Thailand

Avian Pathol. 2023 Sep 18:1-49. doi: 10.1080/03079457.2023.2260322. Online ahead of print.ABSTRACTAbstractChicken is a major reservoir and source of human infections from zoonotic Campylobacter. In southern Thailand, chicken is the most popular meat consumed; however, little is known about the antimicrobial resistance (AMR) of Campylobacter spp. in chicken production systems. We aimed to investigate the prevalence and characteristics of AMR in Campylobacter isolates from commercial broiler and native chicken farms in southern Thailand. A total of 286 Campylobacter isolates (adjusted prevalence: 60.3%, SE ±0.01) were recovered, of which 268 were identified as Campylobacter jejuni and 18 as Campylobacter coli. The adjusted prevalence of resistance was highest for nalidixic acid (98.6%), followed by ciprofloxacin (97.2%), enrofloxacin (96.4%), tetracycline (74.6%), tylosin (38.3%), and erythromycin (10.6%). Commercial broilers had resistance levels 7-11% higher to quinolones and 35-47% lower to macrolides and tetracycline (all p ≤ 0.025) compared to native chicken. Genotypic resistance analyses revealed high prevalence rates (>86%) for gyrA mutant, efflux pump CmeABC, and point mutation A2075G in the 23S rRNA gene. High homogeneity was observed among isolates within study farms. Commercial broilers exhibited higher homogeneity in AMR patterns than native chickens. The best agreement was observed between tetracycline resistance and tet(O) genes (Kappa: k = 0.811, p < 0.0...
Source: Avian Pathology - Category: Pathology Authors: Source Type: research