Single-nucleus transcriptomic mapping uncovers targets for traumatic brain injury

In this study, we meticulously mapped the transcriptome atlas of murine SVZ and its responses to mTBI at the single-cell level. We observed cell-specific gene expression changes following mTBI and unveiled diverse cell-to-cell interaction networks that influence a wide array of cellular processes. Moreover, we reported novel neurogenesis lineage trajectories and related key transcription factors, which we subsequently validated through loss-of-function experiments. Specifically, we validated the role of Tcf7l1, a cell cycle gene regulator, in promoting neural stem cell differentiation towards the neuronal lineage after mTBI, providing a potential target for regenerative medicine. Overall, our study profiles an SVZ transcriptome reference map, which underlies the differential cellular behavior in response to mTBI. The identified key genes and pathways that may ameliorate brain damage or facilitate neural repair serve as valuable resources for drug discovery in the context of mTBI.PMID:37730437 | DOI:10.1101/gr.277881.123

Source: Cell Research - September 20, 2023 Category: Cytology Authors: Qiuyun Yang Lingxuan Zhang Manrui Li Yang Xu Xiaogang Chen Ruixuan Yuan Xiaofeng Ou Min He Miao Liao Lin Zhang Hao Dai Meili Lv Xiaoqi Xie Weibo Liang Xiameng Chen Source Type: research