Development and Sensitivity Determination of < em > 18S rRNA < /em > Gene-specific Fast Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of < em > Acanthamoeba < /em >

CONCLUSION: It has been demonstrated that the LAMP assay designed by targeting 18S rRNA gene of Acanthamoeba has a detection limit of 1 pg of genomic DNA. It is promising that LAMP test is more sensitive and faster than culture method, as well as simple, inexpensive and highly sensitive. For this reason, it is thought that developed test can be applied in the diagnosis of Acanthamoeba in environmental and clinical samples.PMID:37724360 | DOI:10.4274/tpd.galenos.2023.46362
Source: Turkish Society for Parasitology - Category: Parasitology Authors: Source Type: research