Dietary Linoleic Acid: An Omega-6 Fatty Acid Essential for Liver Regeneration in Buffalo Rats

Comp Med. 2023 Aug 31. doi: 10.30802/AALAS-CM-23-000004. Online ahead of print.ABSTRACTRodents are currently the most common animals used for hepatic surgical resection studies that investigate liver regeneration,chronic liver disease, acute liver failure, hepatic metastasis, hepatic function, and hepatic cancer. Our previous workhas shown that dietary consumption of linoleic acid (LA) stimulates the growth of rodent and human tumors in vivo. Herewe compared 3 diets─a 5% corn oil diet (control), a diet deficient in essential fatty acids (EFAD), and an EFAD supplementedwith LA in amounts equal to those in the control diet (EFAD+LA). We hypothesized that consumption of the LA provided inthe EFAD+LA diet would elevate plasma levels of LA and stimulate regeneration in rats after a 70% hepatectomy (HPX), andthat regeneration would not occur in the EFAD rats. Each diet group was comprised of 30 male and 30 female Buffalo rats(BUFF/CrCrl). Rats were fed one of the 3 diets and water ad libitum. After 8 wk on the assigned diet, rats were underwent a70% HPX. On days 4 and 21 after HPX, 30 male and 30 female rats from each diet group were anesthetized for in vivo studyand then were euthanized for tissue collection. For the in vivo study, arterial and venous blood samples were collected fromthe liver. LA-, glucose-, and O2-uptake, and lactate- and CO2-output were significantly higher in LA-replete rats as comparedwith LA-deficient rats. After a 70% HPX, the remaining liver mass in cont...
Source: Comparative Medicine - Category: Zoology Authors: Source Type: research