Investigations of biological activities of aqueous extracts of Prosopis africana in rats with chemical-induced oxidative stress

This study conducted phytochemical screening of the plant extract and investigated its antioxidative and hepatoprotective effects in rats with acetaminophen-induced oxidative stress and liver injury. Aqueous extracts ofP. africana extracts were prepared. Phytochemical analysis of the extract was conducted. Wistar rats were pre-treated with or withoutP. africana extracts (50 –200 mg/kg bw) for 10 days prior to acetaminophen intoxication. Levels of thiobarbituric reactive substances, reduced ascorbic acid, catalase activities, enzyme markers of liver function, and erythrocyte levels of cholesterol and phospholipid were measured. Abundance of all phytochemicals screen ed was observed. Total flavonoid and total phenolic composition were estimated as 64.12 ± 2.11 mg QE/g and 43.17 ± 1.32 GE/g, respectively. Paracetamol (2 g/kg bw) increased serum TBARS (4.1-fold,P <  0.001), catalase activity (3.9-fold,P <  0.001), AST (5.8-fold,P <  0.001), ALT (6.5-fold,P <  0.001), and ALP (12.5-fold,P <  0.001) compared with saline-treated rats. Treatment of rats with extracts ofP. africana inhibited the effect of paracetamol on TBARS (11.4 –45.4%,P <  0.05–0.001), catalase activity (35.0% to 63.3%,P <  0.001), AST (21.9–60.9%,P <  0.001), ALT (20.1–67.7%,P <  0.001), and ALP (26.8–49.8%,P <  0.001) in a dose-treatment manner. Also, levels of reduced ascorbic acid increased in rats pre-treated withP...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research