Toward discovering a novel family of peptides targeting neuroinflammatory states of brain microglia and astrocytes

Microglia are critical to the development and healthy function of the brain and spinal cord, yet are implicated in the active pathology of many neuropsychiatric disorders. Here we proposed in vitro models for surveying, reactive, and primed sub-types of primary rat microglia and mixed microglia/astrocytes. We used a custom 7 amino acid-presenting M13 phage display library (SX7) to discover 58 unique peptides binding to these phenotypes across both glial types and characterized their potential basic local alignment search tool origin. We believe these findings should provide a framework for investigating new biomarkers and microglia/astrocyte interactive peptides. CM, conditioned medium; Dex, dexamethasone; LPS, lipopolysaccharide; Mino, minocycline. AbstractMicroglia are immune-derived cells critical to the development and healthy function of the brain and spinal cord, yet are implicated in the active pathology of many neuropsychiatric disorders. A range of functional phenotypes associated with the healthy brain or disease states has been suggested from in vivo work and were modeled in vitro assurveying,reactive, andprimed sub-types of primary rat microglia and mixed microglia/astrocytes. It was hypothesized that the biomolecular profile of these cells undergoes a phenotypical change as well, and these functional phenotypes were explored for potential novel peptide binders using a custom 7 amino acid-presenting M13 phage library (SX7) to identify unique peptides that bind dif...
Source: Journal of Neurochemistry - Category: Neuroscience Authors: Tags: ORIGINAL ARTICLE Source Type: research