A small H < sub > 2 < /sub > O-soluble ingredient of royal jelly lower cholesterol levels in liver cells by suppressing squalene epoxidase

In this study, we investigated the efficacy of RJ on gene expression of squalene epoxidase (SE) a major enzyme involved in cholesterol biosynthesis in HepG2 cells. We found that the expression of SE was decreased in response to RJ treatment. We also found that the origin of the RJ affected its strength. To find out the active fraction of RJ in cholesterol suppression, we separated RJ into two parts based on the molecular weights using ultrafiltration membrane. We found that the fraction <10kDa from RJ had comparable effect on SE expression, especially its water-soluble part. Taken together, we think RJ suppresses cholesterol by decreasing SE gene expression in liver. The active fraction of RJ in this action is <10kDa in water-soluble form.PMID:36582688 | PMC:PMC9792723 | DOI:10.1016/j.heliyon.2022.e12286
Source: Atherosclerosis - Category: Cardiology Authors: Source Type: research