Vitality ‐Enhanced Dual‐Modal Tracking System Reveals the Dynamic Fate of Mesenchymal Stem Cells for Stroke Therapy

Intravenously transplanted bioengineered mesenchymal stem cell (MSC) achieve high vitality in a harsh environment. With the help of an extracellular antioxidative layer, vitality-enhanced MSCs remodel the microenvironment and improve the therapeutic outcomes. A magnetic resonance imaging and near-infrared fluorescence dual-mode tracking system reveals that MSCs prefer livable niches to maintain high vitality for therapeutic purposes and behave intelligently during the development of pathology and recovery. AbstractMesenchymal stem cell (MSC) therapy via intravenous transplantation exhibits great potential for brain tissue regeneration, but still faces thorny clinical translation challenges as the unknown dynamic fate leads to the contentious therapeutic mechanism and the poor MSC viability in harsh lesions limits therapeutic efficiency. Here, a vitality-enhanced dual-modal tracking system is designed to improve engraftment efficiency and is utilized to noninvasively explore the fate of intravenous transplanted human umbilical cord-derived MSCs during long-term treatment of ischemic stroke. Such a system is obtained by bioorthogonally conjugating magnetic resonance imaging (MRI) contrast and near-infrared fluorescence (NIRF) imaging nanoparticles to metabolic glycoengineered MSCs with a lipoic acid-containing extracellular antioxidative protective layer. The dynamic fates of MSCs in multi-dimensional space-time evolution are digitally detailed for up to 28 days using MRI and N...

Source: Small - October 1, 2022 Category: Nanotechnology Authors: Jianpei Xu, Yuwen Zhang, Yipu Liu, Yang You, Fengan Li, Yu Chen, Laozhi Xie, Shiqiang Tong, Songlei Zhou, Kaifan Liang, Yukun Huang, Gan Jiang, Qingxiang Song, Ni Mei, Fenfen Ma, Xiaoling Gao, He Wang, Jun Chen Tags: Research Article Source Type: research