Improved osteogenic differentiation by extremely low electromagnetic field exposure: possible application for bone engineering

In this study, we investigated the effects of an intermittent ELF-EMF exposure (6  h/day) for the standard differentiation period (28 days) and for 10 days in hPDLSCs in the presence or not of osteogenic differentiation medium (OM). We evaluated cell proliferation, de novo calcium deposition and osteogenic differentiation marker expression in sham and ELF-EMF-exposed cells. Af ter ELF-EMF exposure, compared with sham-exposed, an increase in cell proliferation rate (p <  0.001) and de novo calcium deposition (p <  0.001) was observed after 10 days of exposure. Real-time PCR and Western blot results showed that COL1A1 and RUNX-2 gene expression and COL1A1, RUNX-2 and OPN protein expression were upregulated respectively in the cells exposed to ELF-EMF exposure along with or without OM for 10 days. Altoget her, these results suggested that the promotion of osteogenic differentiation is more efficient in ELF-EMF-exposed hPDLSCs. Moreover, our analyses indicated that there is an early induction of hPDLSC differentiation after ELF-EMF application.
Source: Histochemistry and Cell Biology - Category: Biomedical Science Source Type: research