A label-free colorimetric biosensor utilizing natural material for highly sensitive exosome detection
In this study, we employ sporopollenin microcapsules (SP), a natural biopolymer material derived from pollen, as a substrate for gold nanoparticles (AuNPs). By modifying the SP-Au complex with CD63 aptamers, we develop a label-free colorimetric biosensor for exosome detection. In the absence of exosomes, the SP-Au complex catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), resulting in a color change from colorless to blue. However, the addition of exosomes inhibits the catalytic activity of the SP-Au complex due to coverage of exosomes on AuNPs. This colorimetric biosensor exhibits high sensitivity and select...
Source: Talanta - May 3, 2024 Category: Chemistry Authors: Yibin Wu Zhaojie Wu Wan Xu Ru Zeng Jian Weng Liping Sun Source Type: research
Unravelling the diagnostic methodologies for SARS-CoV-2; the Indispensable need for developing point-of-care testing
Talanta. 2024 May 1;275:126139. doi: 10.1016/j.talanta.2024.126139. Online ahead of print.ABSTRACTThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-caused COVID-19 pandemic that continues to be a global menace and since its emergence in the late 2019, SARS-CoV-2 has been vigorously spreading throughout the globe putting the whole world into a multidimensional calamity. The suitable diagnosis strategies are on the front line of the battle against preventing the spread of infections. Since the clinical manifestation of COVID-19 is shared between various diseases, detection of the unique impacts of the pathogen...
Source: Talanta - May 2, 2024 Category: Chemistry Authors: Erfan Shahbazi Asma Moradi Hamidreza Mollasalehi Seyed Reza Mohebbi Source Type: research
Miniaturized inkjet-printed flexible ion-selective sensing electrodes with the addition of graphene in PVC layer for fast response real-time monitoring applications
Talanta. 2024 Apr 23;275:126107. doi: 10.1016/j.talanta.2024.126107. Online ahead of print.ABSTRACTIn this letter, we propose a miniaturization scheme of inkjet printed ionic sensing electrodes by adding graphene into the ion-selective PVC film not only to reduce the impedance of the ionic liquid layer of the electrode but also to increase the electrode capacitance for the reduction of the response time. Based on the scheme, we present a fully inkjet-printed electrochemical ion-selective sensor comprising a working electrode and reference electrode, which are inkjet-printed Ag NPs/PEDOT:PSS-graphene/PVC-graphene and Ag/AgC...
Source: Talanta - May 2, 2024 Category: Chemistry Authors: Kun-Lin Tsou Yu-Ting Cheng Source Type: research
Unravelling the diagnostic methodologies for SARS-CoV-2; the Indispensable need for developing point-of-care testing
Talanta. 2024 May 1;275:126139. doi: 10.1016/j.talanta.2024.126139. Online ahead of print.ABSTRACTThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-caused COVID-19 pandemic that continues to be a global menace and since its emergence in the late 2019, SARS-CoV-2 has been vigorously spreading throughout the globe putting the whole world into a multidimensional calamity. The suitable diagnosis strategies are on the front line of the battle against preventing the spread of infections. Since the clinical manifestation of COVID-19 is shared between various diseases, detection of the unique impacts of the pathogen...
Source: Talanta - May 2, 2024 Category: Chemistry Authors: Erfan Shahbazi Asma Moradi Hamidreza Mollasalehi Seyed Reza Mohebbi Source Type: research
Miniaturized inkjet-printed flexible ion-selective sensing electrodes with the addition of graphene in PVC layer for fast response real-time monitoring applications
Talanta. 2024 Apr 23;275:126107. doi: 10.1016/j.talanta.2024.126107. Online ahead of print.ABSTRACTIn this letter, we propose a miniaturization scheme of inkjet printed ionic sensing electrodes by adding graphene into the ion-selective PVC film not only to reduce the impedance of the ionic liquid layer of the electrode but also to increase the electrode capacitance for the reduction of the response time. Based on the scheme, we present a fully inkjet-printed electrochemical ion-selective sensor comprising a working electrode and reference electrode, which are inkjet-printed Ag NPs/PEDOT:PSS-graphene/PVC-graphene and Ag/AgC...
Source: Talanta - May 2, 2024 Category: Chemistry Authors: Kun-Lin Tsou Yu-Ting Cheng Source Type: research
A lipid activated color switchable probe for the imaging of diseased aortic valves
Talanta. 2024 Apr 20;275:126069. doi: 10.1016/j.talanta.2024.126069. Online ahead of print.ABSTRACTLipid deposition has been considered one of the key factors in the occurrence of valvular heart disease (VHD) and a great potential target for the diagnosis of VHD. However, the development of lipid imaging technologies and efficient lipid specific probes is in urgent demand. In this work, we have prepared a lipid droplets (LDs) targeted fluorescence probe CPTM based on a push-pull electronic structure for the imaging of diseased aortic valves. CPTM showed obvious twisted intramolecular charge transfer (TICT) effect and its e...
Source: Talanta - May 1, 2024 Category: Chemistry Authors: Weihua Zhuang Junli Li Tianyi Qu Ruochen Shao Jingruo Chen Shufen Li Mao Chen Yunbing Wang Source Type: research
Less interference fluorescence analytical strategy: Bridging substance-triggered ratiometric sensor with convenient preparation and application
Talanta. 2024 Apr 26;275:126102. doi: 10.1016/j.talanta.2024.126102. Online ahead of print.ABSTRACTHigh interference and narrow application range are key of bottleneck of recent fluorescence analysis methods, which limit their wide application in the sensing field. Therefore, to overcome these disadvantages, a ratiometric fluorescence sensing system utilizing berberine (BER) and silver nanoclusters protected by dihydrolipoic acid (DHLA-AgNCs) was constructed for the first time in this work, to achieve determination of BER and daunorubicin (Dau). BER aqueous solution (non-planar conformation) has no fluorescence emission. W...
Source: Talanta - May 1, 2024 Category: Chemistry Authors: Liping Xi Yue Chen Xiaoni Zhang Meiru Liu Jianhui Li Deli Xiao Pierre Dramou Hua He Source Type: research
Highly sensitive and accurate measurement of underivatized phosphoenolpyruvate in plasma and serum via EDTA-facilitated hydrophilic interaction liquid chromatography-tandem mass spectrometry
In this study, a sensitive method for the direct determination of PEP in plasma and serum based on ethylenediaminetetraacetic acid (EDTA)-facilitated hydrophilic interaction liquid chromatography-tandem mass spectrometry was developed. Superior chromatographic retention and peak shapes were achieved using a zwitterionic stationary-phase HILIC column with a metal-inert inner surface. Efficient dechelation of PEP-metal complexes in serum/plasma samples was achieved through the introduction of EDTA, resulting in a significant enhancement of the PEP signal. A PEP isotopically labelled standard was employed as a surrogate analy...
Source: Talanta - May 1, 2024 Category: Chemistry Authors: Danning Shen Yingjie Zhu Jie Mao Runfeng Lin Xin Jiang Longhui Liang Jing Peng Yanqing Cao Suhe Dong Kun He Na Wang Source Type: research
Non-invasive screening and subtyping for breast cancer by serum SERS combined with LGB-DNN algorithms
Talanta. 2024 Apr 27;275:126136. doi: 10.1016/j.talanta.2024.126136. Online ahead of print.ABSTRACTEarly detection of breast cancer and its molecular subtyping is crucial for guiding clinical treatment and improving survival rate. Current diagnostic methods for breast cancer are invasive, time consuming and complicated. In this work, an optical detection method integrating surface-enhanced Raman spectroscopy (SERS) technology with feature selection and deep learning algorithm was developed for identifying serum components and building diagnostic model, with the aim of efficient and accurate noninvasive screening of breast ...
Source: Talanta - May 1, 2024 Category: Chemistry Authors: Qiyi Zhang Yuxiang Lin Duo Lin Xueliang Lin Miaomiao Liu Hong Tao Jinxun Wu Tingyin Wang Chuan Wang Shangyuan Feng Source Type: research
Cascaded autocatalytic hairpin assembly molecular circuit for amplified fluorescent aptamer luteinising hormone assay
Talanta. 2024 Apr 25;275:126150. doi: 10.1016/j.talanta.2024.126150. Online ahead of print.ABSTRACTThe quantitative detection of luteinising hormone (LH) is critical for the study of the physiological mechanism of reproductive function and the assessment of infertility and the clinical treatment of reproductive disorders. However, conventional approaches for LH detection are mostly based on an antibody recognition module with the limitations of sensitivity, simplicity and cost. The development of robust LH sensing methods is therefore highly demanded for facilitating the diagnosis of LH-related diseases. We establish a con...
Source: Talanta - May 1, 2024 Category: Chemistry Authors: Xin Bi Shunmei Li Fang Yang Ruo Yuan Yun Xiang Source Type: research
A comprehensive review of aptamer screening and application for lateral flow strip: Current status and future perspectives
Talanta. 2024 Apr 27;275:126181. doi: 10.1016/j.talanta.2024.126181. Online ahead of print.ABSTRACTThe detection of biomarkers is of great significance for medical diagnosis, food safety, environmental monitoring, and agriculture. However, bio-detection technology at present often necessitates complex instruments, expensive reagents, specialized expertise, and prolonged procedures, making it challenging to fulfill the demand for rapid, sensitive, user-friendly, and economical testing. In contrast, lateral flow strip (LFS) technology offers simple, fast, and visually accessible detection modality, allowing real-time analysi...
Source: Talanta - May 1, 2024 Category: Chemistry Authors: Sixian Wang Zhaoyang Zhou Mingdong Cao Yangwei Pan Yongkang Zhang Yu Fang Qi Sun Xianlu Lei Tao Le Source Type: research
A simple chemiluminescent method for the quantification of exosomes based on horseradish peroxidase adsorbed on two-dimensional nanomaterials
Talanta. 2024 Apr 26;275:126156. doi: 10.1016/j.talanta.2024.126156. Online ahead of print.ABSTRACTThe development of simple methods for the isolation and quantification of exosomes in biological samples is important. By using the typical two-dimensional (2D) nanomaterials, graphene oxide (GO), the present work first studied the interaction of liposomes with the nanocomposites formed by adsorbing HRP on the GO surface and found the presence of liposomes led to the release of HRP from the GO surface to the solution phase triggering the luminol-H2O2 chemiluminescence (CL) reaction to emit light. Benefiting from the similarit...
Source: Talanta - May 1, 2024 Category: Chemistry Authors: Meilin Li Yifan Yu Shanshan Li Feiqian Wang Sile Hong Yinuo Sun Aiping Fan Source Type: research
Comparison of methods for the synthesis of silver nanoparticles stabilized in a solid polymethacrylate matrix
Talanta. 2024 Apr 27;275:126159. doi: 10.1016/j.talanta.2024.126159. Online ahead of print.ABSTRACTSilver nanoparticles were synthesized inside the small plates of transparent polymer medium by reduction of silver cations. The difference in morphology and sizes of nanoparticles synthesized by chemical, photochemical and thermal reduction was revealed, which depends both on the component ratio of the mixture and on the reduction time for the specific component ratio The polymethacrylate matrix itself acts as the stabilizing agent. A colorimetric sensor based on polymethacrylate matrix and silver nanoparticles was developed ...
Source: Talanta - May 1, 2024 Category: Chemistry Authors: Nadezhda V Saranchina Olga A Bazhenova Sofia K Bragina Viktor O Semin Nataliya A Gavrilenko Tatyana N Volgina Mikhail A Gavrilenko Source Type: research
Inflammatory bowel disease alters in vivo distribution of orally administrated nanoparticles: Revealing via SERS tag labeling technique
Talanta. 2024 Apr 27;275:126172. doi: 10.1016/j.talanta.2024.126172. Online ahead of print.ABSTRACTNanoparticles (NPs) could be uptake orally and exposed to digestive tract through various sources such as particulate pollutant, nanomedicine and food additive. Inflammatory bowel disease (IBD), as a global disease, induced disruption of the intestinal mucosal barrier and thus altered in vivo distribution of NPs as a possible consequence. However, related information was relatively scarce. Herein, in vivo distribution of typical silica (SiO2) and titania (TiO2) NPs was investigated in healthy and IBD models at cell and animal...
Source: Talanta - May 1, 2024 Category: Chemistry Authors: Mingyue Tan Yunqing Wang Yunxia Ji Rongchao Mei Xizhen Zhao Jie Song Jinmao You Lingxin Chen Xiaoyan Wang Source Type: research
Endoplasmic reticulum targeted fluorescent probe for real-time monitoring the viscosity changes induced by calcium homeostasis disruption
Talanta. 2024 Apr 27;275:126173. doi: 10.1016/j.talanta.2024.126173. Online ahead of print.ABSTRACTThe endoplasmic reticulum (ER) acts as the major storage site for calcium ions, which are messenger ions for intracellular signaling. Disruption of calcium ion homeostasis can significantly affect the viscosity, polarity and pH of the ER. However, it is still unclear the relationship between the viscosity changes in ER and the imbalance of calcium ion homeostasis. Herein, we developed a novel fluorescent probe, named TPA, for monitoring viscosity changes that specifically targets the endoplasmic reticulum rather than mitochon...
Source: Talanta - May 1, 2024 Category: Chemistry Authors: Ya Meng Lin Jia Yi Shi Gang-Gang Yang Source Type: research
