Effective Plant Virus Enrichment Using Carbon Nanotubes and Microfluidics
In this report, we present a platform for the enrichment and isolation of known or unknown viruses. This platform is based on carbon nanotube arrays inside a microfluidic device that can be a solution for the identification of low titer viruses from plants. Using our microfluidic devices, we achieved enrichment of two economically important viruses, the orthotospovirus, tomato spotted wilt orthotospovirus (TSWV) and the potyvirus, zucchini yellow mosaic virus (ZYMV). The carbon nanotube arrays integrated in these microfluidic devices are capable of trapping viruses discriminated by their size; the virus rich arrays can be ...
Source: Journal of Virological Methods - February 22, 2024 Category: Virology Authors: Nestor Perea-L ópez Francisco Iturralde Chad Vosburg Edwin Rajotte Cristina Rosa Mauricio Terrones Source Type: research
Effective plant virus enrichment using carbon nanotubes and microfluidics
In this report, we present a platform for the enrichment and isolation of known or unknown viruses. This platform is based on carbon nanotube arrays inside a microfluidic device that can be a solution for the identification of low titer viruses from plants. Using our microfluidic devices, we achieved enrichment of two economically important viruses, the orthotospovirus, tomato spotted wilt orthotospovirus (TSWV) and the potyvirus, zucchini yellow mosaic virus (ZYMV). The carbon nanotube arrays integrated in these microfluidic devices are capable of trapping viruses discriminated by their size; the virus rich arrays can be ...
Source: Journal of Virological Methods - February 22, 2024 Category: Virology Authors: Nestor Perea-Lopez Juan Francisco Iturralde Martinez Chad Vosburg Edwin G Rajotte Cristina Rosa Mauricio Terrones Source Type: research
Fermented botanical fertilizer controls bacterial wilt of tomatoes caused by Ralstonia pseudosolanacearum
This study demonstrates the effect of fermented botanical product (FBP) on Ralstonia pseudosolanacearum-induced bacterial wilt disease and unravels its action mechanism. Soaking with diluted FBP solutions (0.1-0.5%) significantly suppressed bacterial wilt in tomato plants, and FBP-treated tomato plants grew well against R. pseudosolanacearum infection. Growth assays showed that FBP had no antibacterial effect but promoted R. pseudosolanacearum growth. In contrast, few or no R. pseudosolanacearum cells were detected in aerial parts of tomato plants grown in FBP-soaked soil. Subsequent infection assays using the chemotaxis-d...
Source: Bioscience, Biotechnology, and Biochemistry - February 21, 2024 Category: Biochemistry Authors: Akiko Hida Nanako Okano Chika Tadokoro Myuji Fukunishi Asmaa Ali Ahmed Kohei Takenaka Yusuke Tateuchi Kotaro Fujioka Hideto Torii Takahisa Tajima Junichi Kato Source Type: research
Fermented botanical fertilizer controls bacterial wilt of tomatoes caused by Ralstonia pseudosolanacearum
This study demonstrates the effect of fermented botanical product (FBP) on Ralstonia pseudosolanacearum-induced bacterial wilt disease and unravels its action mechanism. Soaking with diluted FBP solutions (0.1-0.5%) significantly suppressed bacterial wilt in tomato plants, and FBP-treated tomato plants grew well against R. pseudosolanacearum infection. Growth assays showed that FBP had no antibacterial effect but promoted R. pseudosolanacearum growth. In contrast, few or no R. pseudosolanacearum cells were detected in aerial parts of tomato plants grown in FBP-soaked soil. Subsequent infection assays using the chemotaxis-d...
Source: Bioscience, Biotechnology, and Biochemistry - February 21, 2024 Category: Biochemistry Authors: Akiko Hida Nanako Okano Chika Tadokoro Myuji Fukunishi Asmaa Ali Ahmed Kohei Takenaka Yusuke Tateuchi Kotaro Fujioka Hideto Torii Takahisa Tajima Junichi Kato Source Type: research
Fermented botanical fertilizer controls bacterial wilt of tomatoes caused by Ralstonia pseudosolanacearum
This study demonstrates the effect of fermented botanical product (FBP) on Ralstonia pseudosolanacearum-induced bacterial wilt disease and unravels its action mechanism. Soaking with diluted FBP solutions (0.1-0.5%) significantly suppressed bacterial wilt in tomato plants, and FBP-treated tomato plants grew well against R. pseudosolanacearum infection. Growth assays showed that FBP had no antibacterial effect but promoted R. pseudosolanacearum growth. In contrast, few or no R. pseudosolanacearum cells were detected in aerial parts of tomato plants grown in FBP-soaked soil. Subsequent infection assays using the chemotaxis-d...
Source: Bioscience, Biotechnology, and Biochemistry - February 21, 2024 Category: Biochemistry Authors: Akiko Hida Nanako Okano Chika Tadokoro Myuji Fukunishi Asmaa Ali Ahmed Kohei Takenaka Yusuke Tateuchi Kotaro Fujioka Hideto Torii Takahisa Tajima Junichi Kato Source Type: research
Fermented botanical fertilizer controls bacterial wilt of tomatoes caused by Ralstonia pseudosolanacearum
This study demonstrates the effect of fermented botanical product (FBP) on Ralstonia pseudosolanacearum-induced bacterial wilt disease and unravels its action mechanism. Soaking with diluted FBP solutions (0.1-0.5%) significantly suppressed bacterial wilt in tomato plants, and FBP-treated tomato plants grew well against R. pseudosolanacearum infection. Growth assays showed that FBP had no antibacterial effect but promoted R. pseudosolanacearum growth. In contrast, few or no R. pseudosolanacearum cells were detected in aerial parts of tomato plants grown in FBP-soaked soil. Subsequent infection assays using the chemotaxis-d...
Source: Bioscience, Biotechnology, and Biochemistry - February 21, 2024 Category: Biochemistry Authors: Akiko Hida Nanako Okano Chika Tadokoro Myuji Fukunishi Asmaa Ali Ahmed Kohei Takenaka Yusuke Tateuchi Kotaro Fujioka Hideto Torii Takahisa Tajima Junichi Kato Source Type: research
Viruses, Vol. 16, Pages 322: Two Novel Betarhabdovirins Infecting Ornamental Plants and the Peculiar Intracellular Behavior of the Cytorhabdovirus in the Liana Aristolochia gibertii
Watanabe Kitajima
Two novel members of the subfamily Betarhabdovirinae, family Rhabdoviridae, were identified in Brazil. Overall, their genomes have the typical organization 3′-N-P-P3-M-G-L-5′ observed in mono-segmented plant-infecting rhabdoviruses. In aristolochia-associated cytorhabdovirus (AaCV), found in the liana aristolochia (Aristolochia gibertii Hook), an additional short orphan ORF encoding a transmembrane helix was detected between P3 and M. The AaCV genome and inferred encoded proteins share the highest identity values, consistently < 60%, with their counterparts of t...
Source: Viruses - February 21, 2024 Category: Virology Authors: Pedro Luis Ramos-Gonz ález Maria Amelia Vaz Alexandre Matheus Potsclam-Barro L ígia Maria Lembo Duarte Gianluca L. Michea Gonzalez Camila Chabi-Jesus Alyne F. Ramos Ricardo Harakava Harri Lorenzi Juliana Freitas-Ast úa Elliot Watanabe Kitajima Tags: Article Source Type: research
Use of ginger extract and bacterial inoculants for the suppression of Alternaria solani causing early blight disease in Tomato
Early blight (EB), caused by Alternaria solani, is a serious problem in tomato production. Plant growth-promoting rhizobacteria promote plant growth and inhibit plant disease. The present study explored the bio-e... (Source: BMC Plant Biology)
Source: BMC Plant Biology - February 21, 2024 Category: Biology Authors: Sajjad Hyder, Amjad Shahzad Gondal, Anam Sehar, Aimen Razzaq Khan, Nadia Riaz, Zarrin Fatima Rizvi, Rashid Iqbal, Mohamed S. Elshikh, Khaloud M. Alarjani, Muhammed Habib ur Rahman and Muhammad Rizwan Tags: Research Source Type: research
A widely targeted metabolite modificomics strategy for modified metabolites identification in tomato
In this study, a widely targeted metabolite modificomics (WTMM) strategy was developed based on ultra-high performance liquid chromatography-quadrupole-linear ion trap (UHPLC-Q-Trap) and UHPLC-Q-Exactive-Orbitrap (UHPLC-QE-Orbitrap), which greatly improved the detection sensitivity and the efficiency of identification of modified metabolites. A metabolite modificomics study was carried out using tomato as a model, and over 34,000 signals with MS2 information were obtained from approximately 232 neutral loss transitions. Unbiased metabolite profiling was also performed by utilizing high-resolution mass spectrometry data to ...
Source: Journal of Integrative Plant Biology - February 20, 2024 Category: Biology Authors: Jun Yang Ridong Chen Chao Wang Chun Li Weizhen Ye Zhonghui Zhang Shouchuang Wang Source Type: research
A widely targeted metabolite modificomics strategy for modified metabolites identification in tomato
In this study, a widely targeted metabolite modificomics (WTMM) strategy was developed based on ultra-high performance liquid chromatography-quadrupole-linear ion trap (UHPLC-Q-Trap) and UHPLC-Q-Exactive-Orbitrap (UHPLC-QE-Orbitrap), which greatly improved the detection sensitivity and the efficiency of identification of modified metabolites. A metabolite modificomics study was carried out using tomato as a model, and over 34,000 signals with MS2 information were obtained from approximately 232 neutral loss transitions. Unbiased metabolite profiling was also performed by utilizing high-resolution mass spectrometry data to ...
Source: Journal of Integrative Plant Biology - February 20, 2024 Category: Biology Authors: Jun Yang Ridong Chen Chao Wang Chun Li Weizhen Ye Zhonghui Zhang Shouchuang Wang Source Type: research
A widely targeted metabolite modificomics strategy for modified metabolites identification in tomato
In this study, a widely targeted metabolite modificomics (WTMM) strategy was developed based on ultra-high performance liquid chromatography-quadrupole-linear ion trap (UHPLC-Q-Trap) and UHPLC-Q-Exactive-Orbitrap (UHPLC-QE-Orbitrap), which greatly improved the detection sensitivity and the efficiency of identification of modified metabolites. A metabolite modificomics study was carried out using tomato as a model, and over 34,000 signals with MS2 information were obtained from approximately 232 neutral loss transitions. Unbiased metabolite profiling was also performed by utilizing high-resolution mass spectrometry data to ...
Source: Journal of Integrative Plant Biology - February 20, 2024 Category: Biology Authors: Jun Yang Ridong Chen Chao Wang Chun Li Weizhen Ye Zhonghui Zhang Shouchuang Wang Source Type: research
MicroRNA396 negatively regulates shoot regeneration in tomato
Hortic Res. 2024 Jan 2;11(2):uhad291. doi: 10.1093/hr/uhad291. eCollection 2024 Feb.ABSTRACTNumerous studies have been dedicated to genetically engineering crops to enhance their yield and quality. One of the key requirements for generating genetically modified plants is the reprogramming of cell fate. However, the efficiency of shoot regeneration during this process is highly dependent on genotypes, and the underlying molecular mechanisms remain poorly understood. Here, we identified microRNA396 (miR396) as a negative regulator of shoot regeneration in tomato. By selecting two genotypes with contrasting shoot regeneration...
Source: Cell Research - February 19, 2024 Category: Cytology Authors: Su-Jin Park Ji-Sun Park Jin Ho Yang Ki-Beom Moon Seung Yong Shin Jae-Heung Jeon Hyun-Soon Kim Hyo-Jun Lee Source Type: research
Role of aminopeptidase N ‐like in the acquisition of begomoviruses by Bemisia tabaci, the whitefly vector
In this study, we identified an aminopeptidase N-like protein (BtAPN) inB. tabaci Asia II 1, an efficient vector of SLCMV, which is involved in the SLCMV transmission process. Through the use of glutathione S-transferase pull-down assay and LC-MS/MS analysis, we demonstrated the interaction between BtAPN and the coat protein (CP) of SLCMV. This interaction was further confirmedin vitro, and we observed an induction ofBtAPN gene expression following SLCMV infection. By interfering with the function of BtAPN, the quantities of SLCMV were significantly reduced in various parts ofB. tabaci Asia II 1, including the wh...
Source: Insect Science - February 19, 2024 Category: Biology Authors: Yun ‐Yun Fan,
Yao Chi,
Na Chen,
Wilmer J. Cuellar,
Xiao‐Wei Wang Tags: ORIGINAL ARTICLE Source Type: research
Engineering of stable infectious cDNA constructs of a fluorescently tagged tomato chlorosis virus
This study demonstrates the potential of ToCV-GFP as a valuable tool for the visual tracking of infection and movement of criniviruses in living plants.PMID:38364352 | DOI:10.1016/j.virol.2024.110010 (Source: Virology)
Source: Virology - February 16, 2024 Category: Virology Authors: Sun-Jung Kwon Ye-Ji Lee Young-Eun Cho Hee-Seong Byun Jang-Kyun Seo Source Type: research
Engineering of stable infectious cDNA constructs of a fluorescently tagged tomato chlorosis virus
This study demonstrates the potential of ToCV-GFP as a valuable tool for the visual tracking of infection and movement of criniviruses in living plants.PMID:38364352 | DOI:10.1016/j.virol.2024.110010 (Source: Virology)
Source: Virology - February 16, 2024 Category: Virology Authors: Sun-Jung Kwon Ye-Ji Lee Young-Eun Cho Hee-Seong Byun Jang-Kyun Seo Source Type: research
