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ARL15 and its multiple disease associations: Emerging functions and potential therapeutic applications
Curr Protein Pept Sci. 2023 Sep 15. doi: 10.2174/1389203724666230915123217. Online ahead of print.ABSTRACTARL15 is a member of the RAS superfamily of small GTPases and is associated with several metabolic traits, including increased risk of diabetes, rheumatoid arthritis and lipid metabolism disorders. The ARL15 gene encodes for an uncharacterized small GTP binding protein. Its precise role in human physiology remains unknown, but several genetic association studies have recognized different variants in this gene to be statistically associated with numerous traits and complex diseases. Here, we provided the unique features...
Source: Current Protein and Peptide Science - September 18, 2023 Category: Biochemistry Authors: Manisha Saini Varnita Anand Aditya Sharma Anuj Pandey B K Thelma Suman Kundu Source Type: research
CRISPR/Cas9-Based Edition of Frataxin Gene in Dictyostelium discoideum
Biochem J. 2023 Sep 18:BCJ20230244. doi: 10.1042/BCJ20230244. Online ahead of print.ABSTRACTIn this paper we describe the development of a Dictyostelium discoideum strain deficient in frataxin protein (FXN). We investigated the conservation of function between humans and D. discoideum and showed that DdFXN can substitute the human version in the interaction and activation of the Fe-S assembly supercomplex. We edited the D. discoideum fxn locus and isolated a defective mutant, clone 8, which presents landmarks of frataxin deficiency, such as a decrease in Fe-S cluster-dependent enzymatic functions, growth rate reduction, an...
Source: The Biochemical Journal - September 18, 2023 Category: Biochemistry Authors: Hernan Gustavo Gentili Mar ía Florencia Pignataro Justo Facundo Olmos Mar ía Florencia Paván Lorena Itat í Ibáñez Javier Santos Francisco Velazquez Duarte Source Type: research
Efficient TNBC immunotherapy by dual reprogramming tumor-infiltrating dendritic cells and tumor-associated macrophages with stimulus-responsive miR155 nanocomplexes
In this study, pH/ROS-responsive microRNA-155 (miR155) nanocomplexes (MiR@PCPmP NPs) were developed to reprogram TIDCs and TAMs for efficient TNBC immunotherapy. This nanoplatform was based on a pH/ROS cleavable copolymer of poly(ethylene glycol)-carboxydimethyl maleate-poly(ethyleneimine)-peroxalate ester-poly(ε-caprolactone) grafted with mannose moieties (PEG-CDM-PEI[Man]-ox-PCL) which self-assembled with miRNA to form nanocomplexes. In the tumor microenvironment, the nanocomplexes showed selective cellular uptake by TIDCs and TAMs through PEG detachment and mannose exposure, followed by efficient endosomal escape, cyto...
Source: International Journal of Biological Macromolecules - September 18, 2023 Category: Biochemistry Authors: Zhenghui Jing Yanfeng Li Jinxiao Song Xinlong Zang Source Type: research
Molecular action mechanisms of two novel and selective calcium release-activated calcium channel antagonists
Int J Biol Macromol. 2023 Sep 16:126937. doi: 10.1016/j.ijbiomac.2023.126937. Online ahead of print.ABSTRACTThe prototypical calcium release-activated calcium (CRAC) channel, composed of STIM1 and Orai1, is a sought-after drug target for treating autoimmune disorders. Herein, we identified two novel and selective CRAC channel inhibitors, the indole-like compound C63368 and pyrazole core-containing compound C79413, potently and reversibly inhibiting the CRAC channel with low micromolar IC50s and sparing various off-target ion channels. These two compounds did not inhibit STIM1 activation or its coupling with Orai1, nor did ...
Source: International Journal of Biological Macromolecules - September 18, 2023 Category: Biochemistry Authors: Xiangjin Kong Feifan Wang Yan Chen Xinyao Liang Yuan Yin Hao Liu Guoqing Luo Yinping Li Songping Liang Youjun Wang Zhonghua Liu Cheng Tang Source Type: research
Structural elucidation and physicochemical properties of litchi polysaccharide with the promoting effect on exopolysaccharide production by Weissella confusa
Int J Biol Macromol. 2023 Sep 16:126944. doi: 10.1016/j.ijbiomac.2023.126944. Online ahead of print.ABSTRACTExopolysaccharide (EPS), as a secondary metabolite of microorganisms, has been commonly used in the dairy industry to replace the traditional stabilizers. However, the EPS production by microorganism is generally low, which limits its application. A litchi polysaccharide (Lzp2-2) with the promoting effect on EPS production by Weissella confusa was purified. The SEM and FT-IR analysis indicated that Lzp2-2 displayed a compact netlike structure and typical bands of carbohydrates. The structure of Lzp2-2 was further elu...
Source: International Journal of Biological Macromolecules - September 18, 2023 Category: Biochemistry Authors: Teng-Gen Hu Feng-Xiang Tan Lu Li Ke-Jing An Bo Zou Jing Wen Ji-Jun Wu Geng-Sheng Xiao Yuan-Shan Yu Yu-Juan Xu Source Type: research
Anti-inflammatory potential of ulvan
Int J Biol Macromol. 2023 Sep 16:126936. doi: 10.1016/j.ijbiomac.2023.126936. Online ahead of print.ABSTRACTGreen seaweeds are a widespread group of marine macroalgae that could be regarded as biorenewable source of valuable compounds, in particular sulfated polysaccharides like ulvans with interesting biological properties. Among them, anti-inflammatory activity represents an interesting target, since ulvans could potentially avoid side effects of conventional therapies. However, a great variability in ulvan content, composition, structure and properties occurs depending on seaweed specie and growth and processing conditi...
Source: International Journal of Biological Macromolecules - September 18, 2023 Category: Biochemistry Authors: Noelia Fl órez-Fernández Arianna Rodr íguez-Coello Thomas Latire Nathalie Bourgougnon M Dolores Torres Manuela Buj án Alexandra Mu íños Antonio Mui ños Rosa Meijide-Fa ílde Francisco J Blanco Carlos Vaamonde-Garc ía Herminia Dom ínguez Source Type: research
Green preparation of magnetic ferroferric oxide-polyvinyl alcohol-alginate coated UiO-67 nanospheres: Characterization, adsorption properties and adsorption mechanism of methylene blue
Int J Biol Macromol. 2023 Sep 16:126967. doi: 10.1016/j.ijbiomac.2023.126967. Online ahead of print.ABSTRACTIn this paper, a kind of magnetic ferric oxide/polyvinyl alcohol/calcium alginate/UiO-67 (Fe3O4/PVA/CA/UiO-67) nanospheres with homogeneous surface interconnecting structures was prepared by using macromolecular polymer polyvinyl alcohol and sodium alginate as carriers and zirconium organic skeleton as nanocrystals. The properties of magnetic nanospheres were studied by SEM, FT-IR, TGA, XRD, BET, VSM and Zeta potential. The impression of diverse temperatures, MB concentrations, interaction time, pH, and magnetic aero...
Source: International Journal of Biological Macromolecules - September 18, 2023 Category: Biochemistry Authors: Zhenyu Jing Yanhui Li Qiuju Du Xinxin Pi Yuqi Wang Shiyong Zhao Yonghui Jin Source Type: research
Comprehensive review on single and dual modification of starch: Methods, properties and applications
Int J Biol Macromol. 2023 Sep 16:126952. doi: 10.1016/j.ijbiomac.2023.126952. Online ahead of print.ABSTRACTStarch is a natural, renewable, affordable, and easily available polymer used as gelling agents, thickeners, binders, and potential raw materials in various food products. Due to these techno-functional properties of starch, food and non-food industries are showing interest in developing starch-based food products such as films, hydrogels, starch nanoparticles, and many more. However, the application of native starch is limited due to its shortcomings. To overcome these problems, modification of starch is necessary. ...
Source: International Journal of Biological Macromolecules - September 18, 2023 Category: Biochemistry Authors: Bharati Kumari Nandan Sit Source Type: research
Biomass-based indole derived fluorescence sensor composited with cellulose paper: Detection of picric acid in food and environment samples
Int J Biol Macromol. 2023 Sep 16:126963. doi: 10.1016/j.ijbiomac.2023.126963. Online ahead of print.ABSTRACTPicric acid (PA) is highly water-soluble, the fact makes it stand out as the most hazardous environment pollutant. Therefore, accurate determination of PA is of great significance for human health and environmental protection. Herein, a novel indole-based fluorescent sensor (H1) with good water solubility and fluorescence stability was reported. H1 exhibited 'turn-off' fluorescence response for PA with fast reaction rate (<30 s), unique specificity and excellent selectivity and high sensitivity (limit of detection...
Source: International Journal of Biological Macromolecules - September 18, 2023 Category: Biochemistry Authors: Yingying Zhang Xiaoci Cui Xin Wang Xiuyuan Feng Weixia Cheng Ranhua Xiong Chaobo Huang Source Type: research
Diosgenin prevents dexamethasone-induced myotube atrophy in C2C12 cells
Arch Biochem Biophys. 2023 Sep 16:109759. doi: 10.1016/j.abb.2023.109759. Online ahead of print.ABSTRACTSeveral pathophysiological abnormalities, including a sedentary lifestyle, chronic diseases, and oxidative stress, can contribute to muscle atrophy triggered by an imbalance in muscle protein synthesis and degradation. Resolving muscle atrophy is a critical issue as it can reduce the quality of life. Here, one of the promising functional food factors, diosgenin (a steroidal sapogenin) showed strong preventive activities against dexamethasone (Dex)-induced muscle atrophy, as determined by the expression levels and morphol...
Source: Archives of Biochemistry and Biophysics - September 18, 2023 Category: Biochemistry Authors: Yasukiyo Yoshioka Shiori Oishi Keita Onoda Katsumi Shibata Noriyuki Miyoshi Source Type: research
CST2 is activated by RUNX1 and promotes pancreatic cancer progression by activating PI3K/AKT pathway
In this study, Gene Expression Profiling Interactive Analysis was performed using the Cancer Genome Atlas (TCGA) dataset containing pancreatic tumor samples and normal tissues. The functional role of CST2 in pancreatic cells was investigated by gene knockdown in vitro and in mouse xenograft tumor model. We found that CST2 was overexpressed in pancreatic tumor samples and cell lines. The knockdown of CST2 led to reduced proliferation, migration, and invasion, while apoptotic events were increased upon CST2 silencing in pancreatic cancer cells. In the xenograft mouse model of pancreatic cells, CST2 knockdown also retarded tu...
Source: Archives of Biochemistry and Biophysics - September 18, 2023 Category: Biochemistry Authors: Rongwen Ou Chengjie Lin Youting Chen Source Type: research
Schweinfurthiamide, a new alkaloid isolated from the tuber roots of < em > Asparagus flagellaris < /em > Baker (Liliaceae)
Nat Prod Res. 2023 Sep 18:1-7. doi: 10.1080/14786419.2023.2257358. Online ahead of print.ABSTRACTThe phytochemical investigations of the tuber roots of Asparagus flagellaris led to the isolation of a new alkaloid, schweinfurthiamide (1) and eight known compounds. The structures of the isolated compounds were elucidated using spectroscopic techniques 1D and 2D NMR (1H, 13C, COSY, HMBC, HMQC, HSQC-TOCSY). The absolute configuration of 1 was unambiguously determined using DP4+ calculations. Compound 1 showed significant cytotoxicity against MCF-7 (breast cancer), NCI-H460 (lung cancer), and Hela (cervical cancer) cancer cell ...
Source: Natural Product Research - September 18, 2023 Category: Biochemistry Authors: Elodie Nguelami Kamdeu Ad èle Nicaise Menye Nomo Abdou Tchoukoua Orleans Ngomo Pascal Djimeli Douanla Marguerite Hortence Tchuendem Kenmogne Yoshihito Shiono Turibio Kuiate Tabopda Source Type: research
SERS-substrates based on ZnO nanoflowers prepared by green synthesis
Anal Biochem. 2023 Sep 16:115328. doi: 10.1016/j.ab.2023.115328. Online ahead of print.ABSTRACTZnO nanoparticles (NPs) with a flower-like morphology, synthesized by an affordable colloidal route using an aqueous fungi extract of Ganoderma lucidum as a reducing agent and stabilizer, are investigated as SERS-substrate. Each "flower" has large effective surface that is preserved at packing particles into a dense film and thus exhibits an advantageous property for SERS and similar sensing applications. The mycoextract used in our low-cost and green synthesis as surface stabilizer allows subsequent deposition of metal NPs or la...
Source: Analytical Biochemistry - September 18, 2023 Category: Biochemistry Authors: Volodymyr Dzhagan Oleksandr Smirnov Mariia Kovalenko Oleksandr Gudymenko Nazar Mazur Olga Kapush Mykola Skoryk Yaroslav Pirko Alla Yemets Mykhailo Valakh Petro Shepeliavyi Volodymyr Yukhymchuk Source Type: research
Optimization of microplate-based phenol-sulfuric acid method and application to the multi-sample measurements of cellulose nanofibers
Anal Biochem. 2023 Sep 16:115329. doi: 10.1016/j.ab.2023.115329. Online ahead of print.ABSTRACTThe phenol-sulfuric acid (PSA) method is a widely used colorimetric method for determining the total saccharides. Microplate-based PSA methods have been developed to handle a large number of samples and reduce the use of hazardous chemicals. However, the optimal procedures and measurement conditions for this method have not yet been fully established. To address this gap, we investigated the optimal procedure for microplate-based PSA. In addition to glucose (Glc), two types of cellulose nanofibers (CNFs) were also evaluated as th...
Source: Analytical Biochemistry - September 18, 2023 Category: Biochemistry Authors: Isamu Ogura Miyuki Sugiyama Rie Tai Hiroyuki Mano Tomohiko Matsuzawa Source Type: research
A fluorescent Ponceau S-based total protein normalization method for conventional and challenging immunoblot samples
Anal Biochem. 2023 Sep 16:115330. doi: 10.1016/j.ab.2023.115330. Online ahead of print.ABSTRACTImmunoblotting normalization issues have been recently overcome by whole lane staining. Herein, we are taking advantage of these recent advances and of the fluorophore status of the Ponceau S stain in order to combine the advantages of whole lane staining and fluorescence. By Ponceau S excitation at 488nm, we identify the so-called 'fluorescent Ponceau' method as more linear, more sensitive and more repeatable than the others in protein lysates of distant biochemical profiles (cells, human and mouse tissues). This essentially cos...
Source: Analytical Biochemistry - September 18, 2023 Category: Biochemistry Authors: Claire Verzeroli Charlotte A Hernandez Fabien Zoulim Romain Parent Source Type: research
