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Sterically Enhanced Control of Enzyme-Assisted DNA Assembly
Chembiochem. 2023 Sep 8:e202300361. doi: 10.1002/cbic.202300361. Online ahead of print.ABSTRACTTraditional methods for the assembly of functionalised DNA structures, involving enzyme restriction and modification, present difficulties when working with small DNA fragments (<100bp), in part due to a lack of control over enzymatic action during the DNA modification process. This limits the design flexibility and range of accessible DNA structures. Here, we show that these limitations can be overcome by introducing chemical modifications into the DNA, which spatially restrict enzymatic activity. This approach, Sterically Co...
Source: Chembiochem - September 8, 2023 Category: Biochemistry Authors: Oliver J Irving Lauren Matthews Steven Coulthard Robert K Neely Melissa M Grant Tim Albrecht Source Type: research
Sensors, Vol. 23, Pages 7766: Anthropogenic Object Localization: Evaluation of Broad-Area High-Resolution Imagery Scans Using Deep Learning in Overhead Imagery
We present algorithms for localizing object detection results, as well as a methodology for the evaluation of the results of broad-area scans. Our research explores the challenges of transitioning these models out of the training&ndash;validation laboratory setting and into the real-world application domain. We show a scalable approach to leverage state-of-the-art deep convolutional neural networks for the search, detection, and annotation of objects within large swaths of imagery, with the ultimate goal of providing a methodology for evaluating object detection machine learning models in real-world scenarios.
Source: Sensors - September 8, 2023 Category: Biotechnology Authors: J. Alex Hurt Ilinca Popescu Curt H. Davis Grant J. Scott Tags: Article Source Type: research
Progress Toward Measles Elimination - African Region, 2017-2021
MMWR Morb Mortal Wkly Rep. 2023 Sep 8;72(36):985-991. doi: 10.15585/mmwr.mm7236a3.ABSTRACTWorldwide, measles remains a major cause of disease and death; the highest incidence is in the World Health Organization African Region (AFR). In 2011, the 46 AFR member states established a goal of regional measles elimination by 2020; this report describes progress during 2017-2021. Regional coverage with a first dose of measles-containing vaccine (MCV) decreased from 70% in 2017 to 68% in 2021, and the number of countries with ≥95% coverage decreased from six (13%) to two (4%). The number of countries providing a second MCV dose ...
Source: MMWR Morb Mortal Wkl... - September 7, 2023 Category: Epidemiology Authors: Balcha G Masresha Cynthia Hatcher Emmaculate Lebo Patricia Tanifum Ado M Bwaka Anna A Minta Sebastien Antoni Gavin B Grant Robert T Perry Patrick O'Connor Source Type: research
Experience and Impact of COVID-19 on a Newly Formed Rural University Medical Office: Survey Study
CONCLUSIONS: The findings suggest that COVID-19 made the overall administration of programs more complex and drew attention from other medical and public health programs. While remoteness is appropriate for some medical education tasks, it is less appropriate for clinical learning. Remoteness presents an especially large challenge to clinical education. Employees now have expectations for remoteness to be built into programs and workplaces. Program administrators will have to integrate remoteness' benefits and drawbacks into their organization for the foreseeable future.PMID:37676708 | DOI:10.2196/48299
Source: Rural Remote Health - September 7, 2023 Category: Rural Health Authors: Mark Benton Source Type: research
Municipal separate storm sewer system (MS4) dry weather flows and potential flow sources as assessed by conventional and advanced bacterial analyses
Environ Pollut. 2023 Sep 5:122521. doi: 10.1016/j.envpol.2023.122521. Online ahead of print.ABSTRACTMunicipal separate storm sewer systems (MS4s) function in urbanized areas to convey flows during both wet weather (i.e., stormwater) and dry weather (i.e., urban runoff as well as subsurface sources of flow) to receiving waters. While urban stormwater is known to contain microbial and chemical pollutants, MS4 dry weather flows, or non-stormwater discharges (NSWDs), are much less studied, although they are also known to contain pollutants, especially when these flows include raw sewage. In addition, some natural NSWDs (e.g., ...
Source: Environmental Pollution - September 7, 2023 Category: Environmental Health Authors: Dong Li Laurie C Van De Werfhorst Jared Ervin Aaron Poresky Brandon Steets Cindy Rivers Grant Sharp Jen Smith Patricia A Holden Source Type: research
Progress Toward Measles Elimination - African Region, 2017-2021
MMWR Morb Mortal Wkly Rep. 2023 Sep 8;72(36):985-991. doi: 10.15585/mmwr.mm7236a3.ABSTRACTWorldwide, measles remains a major cause of disease and death; the highest incidence is in the World Health Organization African Region (AFR). In 2011, the 46 AFR member states established a goal of regional measles elimination by 2020; this report describes progress during 2017-2021. Regional coverage with a first dose of measles-containing vaccine (MCV) decreased from 70% in 2017 to 68% in 2021, and the number of countries with ≥95% coverage decreased from six (13%) to two (4%). The number of countries providing a second MCV dose ...
Source: MMWR Morb Mortal Wkl... - September 7, 2023 Category: Epidemiology Authors: Balcha G Masresha Cynthia Hatcher Emmaculate Lebo Patricia Tanifum Ado M Bwaka Anna A Minta Sebastien Antoni Gavin B Grant Robert T Perry Patrick O'Connor Source Type: research
Municipal separate storm sewer system (MS4) dry weather flows and potential flow sources as assessed by conventional and advanced bacterial analyses
Environ Pollut. 2023 Sep 5:122521. doi: 10.1016/j.envpol.2023.122521. Online ahead of print.ABSTRACTMunicipal separate storm sewer systems (MS4s) function in urbanized areas to convey flows during both wet weather (i.e., stormwater) and dry weather (i.e., urban runoff as well as subsurface sources of flow) to receiving waters. While urban stormwater is known to contain microbial and chemical pollutants, MS4 dry weather flows, or non-stormwater discharges (NSWDs), are much less studied, although they are also known to contain pollutants, especially when these flows include raw sewage. In addition, some natural NSWDs (e.g., ...
Source: Environmental Pollution - September 7, 2023 Category: Environmental Health Authors: Dong Li Laurie C Van De Werfhorst Jared Ervin Aaron Poresky Brandon Steets Cindy Rivers Grant Sharp Jen Smith Patricia A Holden Source Type: research
Progress Toward Measles Elimination - African Region, 2017-2021
MMWR Morb Mortal Wkly Rep. 2023 Sep 8;72(36):985-991. doi: 10.15585/mmwr.mm7236a3.ABSTRACTWorldwide, measles remains a major cause of disease and death; the highest incidence is in the World Health Organization African Region (AFR). In 2011, the 46 AFR member states established a goal of regional measles elimination by 2020; this report describes progress during 2017-2021. Regional coverage with a first dose of measles-containing vaccine (MCV) decreased from 70% in 2017 to 68% in 2021, and the number of countries with ≥95% coverage decreased from six (13%) to two (4%). The number of countries providing a second MCV dose ...
Source: MMWR Morb Mortal Wkl... - September 7, 2023 Category: Epidemiology Authors: Balcha G Masresha Cynthia Hatcher Emmaculate Lebo Patricia Tanifum Ado M Bwaka Anna A Minta Sebastien Antoni Gavin B Grant Robert T Perry Patrick O'Connor Source Type: research
Nanoscale dynamic mechanical analysis on interfaces of biological composites
J Mech Behav Biomed Mater. 2023 Aug 25;146:106091. doi: 10.1016/j.jmbbm.2023.106091. Online ahead of print.ABSTRACTBiological composites incorporate structural arrays of rigid-elastic reinforcements made of minerals or crystalline biopolymers, which are connected by thin, compliant, and viscoelastic macromolecular matrix material. The near-interface regions of these biological composites grant them energy dissipation capabilities against dynamic mechanical loadings, which promote various biomechanical functions such as impact adsorption, fracture toughness, and mechanical signal filtering. Here, we employ theoretical model...
Source: Journal of the Mechanical Behavior of Biomedical Materials - September 6, 2023 Category: Materials Science Authors: Ofer Braunshtein Liat Levavi Igor Zlotnikov Benny Bar-On Source Type: research
Diverse Durham collection phages demonstrate complex BREX defense responses
Appl Environ Microbiol. 2023 Sep 5:e0062323. doi: 10.1128/aem.00623-23. Online ahead of print.ABSTRACTBacteriophages (phages) outnumber bacteria ten-to-one and cause infections at a rate of 1025 per second. The ability of phages to reduce bacterial populations makes them attractive alternative antibacterials for use in combating the rise in antimicrobial resistance. This effort may be hindered due to bacterial defenses such as Bacteriophage Exclusion (BREX) that have arisen from the constant evolutionary battle between bacteria and phages. For phages to be widely accepted as therapeutics in Western medicine, more must be u...
Source: Applied and Environmental Microbiology - September 5, 2023 Category: Microbiology Authors: Abigail Kelly Sam C Went Giuseppina Mariano Liam P Shaw David M Picton Samuel J Duffner Isabel Coates Ryan Herdman-Grant Julia Gordeeva Alena Drobiazko Artem Isaev Yan-Jiun Lee Yvette Luyten Richard D Morgan Peter Weigele Konstantin Severinov Nicolas Wenn Source Type: research
