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Cancer: Breast Cancer
Therapy: Gene Therapy

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Total 32 results found since Jan 2013.

Simultaneous Delivery of Doxorubicin and EZH2 ‐Targeting siRNA by Vortex Magnetic Nanorods Synergistically Improved Anti‐Tumor Efficacy in Triple‐Negative Breast Cancer
A novel nanomedicine is synthesized based on Fe3O4 vortex nanorods loaded with doxorubicin (DOX) and EZH2 siRNAs and coated with a macrophage membrane. It shows a preferential tumor enrichment profile, thus leading to highly efficient drug delivery at the tumor site. Combining chemotherapy and gene therapy exhibits synergistic antitumor activity in a preclinical TNBC model. AbstractTriple-negative breast cancer (TNBC), one of the most aggressive types of breast cancer, currently lacks a targeted therapy and has a high clinical recurrence rate. The present study reports an engineered magnetic nanodrug based on Fe3O4 vortex ...
Source: Small - June 28, 2023 Category: Nanotechnology Authors: Yunshu Lu, Fenfen Gu, Yuwei Ma, Ruonan Li, Yi Luo, Xianhong Da, Lan Jiang, Xiang Li, Yan Liu Tags: Research Article Source Type: research

Suppression of mTOR expression by siRNA leads to cell cycle arrest and apoptosis induction in MDA-MB-231 breast cancer cells
CONCLUSION: With these results, we can conclude that mTOR-siRNA exerts direct 'anti-breast cancer' activity propagated by the S6K-GSK-3β- caspase 3 mediated apoptosis and by inducing cell cycle arrest.PMID:36999418 | DOI:10.2174/1566523223666230329085606
Source: Current Gene Therapy - March 31, 2023 Category: Genetics & Stem Cells Authors: Roja Sahu Shivesh Jha Shakti Pattanayak Source Type: research

Host-Guest Interaction-Based Dual response core/shell nanoparticles as efficient siRNA carrier for killing breast cancer cells
Colloids Surf B Biointerfaces. 2021 Jun 11;205:111918. doi: 10.1016/j.colsurfb.2021.111918. Online ahead of print.ABSTRACTHow to overcome multiple obstacles to achieve the efficient and safe delivery of therapeutic genes is still the key to gene therapy. To address this issue, a cationic carrier consisting of polyamide-amine (HPAA-peptide-Fc) modified by an enzyme-responsive polypeptide as the core and hyperbranched polyglycerol derivative (CD-HPG) as the shell was synthesized by self-assembly. The obtained HPAA-peptide-HPG could form the compact nanocomplex with siPlk1, thus confirming the stable load of genes and subsequ...
Source: Colloids and Surfaces - June 18, 2021 Category: Biotechnology Authors: Jinglan Liang Chengguang Wu Xiaoyan Zhou Yunfeng Shi Jun Xu Xiang Cai Tingling Fu Dong Ma Wei Xue Source Type: research

Metformin-conjugated micellar system with intratumoral pH responsive de-shielding for co-delivery of doxorubicin and nucleic acid.
Abstract A novel PMet-P(cdmPEG2K) polymeric micellar carrier was developed for tumor-targeted co-delivery of DOX and nucleic acids (NA), based on polymetformin and a structure designed to lose the PEG shell in response to the acidic extracellular tumor environment. NA/DOX co-loaded micelleplexes exhibited enhanced inhibition of cell proliferation compared to DOX-loaded micelles, and displayed a higher level of cytotoxicity at an acidic pH (6.8) which mimicks the tumor microenvironment. The PMet-P(cdmPEG2K) micelles achieved significantly improved transfection with either a reporter plasmid or Cy3-siRNA, and enhanc...
Source: Biochemical Pharmacology - February 2, 2021 Category: Drugs & Pharmacology Authors: Liu Y, Sun J, Huang Y, Chen Y, Li J, Liang L, Xu J, Wan Z, Zhang B, Li Z, Li S Tags: Biochem Pharmacol Source Type: research

Proteomics evaluation of MDA-MB-231 breast cancer cells in response to RNAi-induced silencing of hPTTG
This study aims to evaluate cell proliferation and the downstream expression pattern of hPTTG1 gene at the mRNA and protein levels after specific down-regulation of hPTTG1 by siRNA.Main methodsThe human breast cancer MDA-MB-231 cell line was transfected with siRNA against hPTTG1. The mRNA and protein expression levels were examined by Real-time PCR and Western blot, respectively. The cell proliferation was assayed by MTS. To investigate the pattern of protein expression, total cellular protein was analyzed by 2D gel electrophoresis and mass spectroscopy. Subsequently, the possible biological consequences were determined by...
Source: Life Sciences - September 13, 2019 Category: Biology Source Type: research

Combination of photodynamic therapy and gene silencing achieved through the hierarchical self-assembly of porphyrin-siRNA complexes
Publication date: Available online 31 July 2019Source: International Journal of PharmaceuticsAuthor(s): Nabila Laroui, Maëva Coste, Laure Lichon, Yannick Bessin, Magali Gary-Bobo, Geneviève Pratviel, Colin Bonduelle, Nadir Bettache, Sébastien UlrichAbstractIn this work, we implemented a supramolecular approach in order to combine photodynamic therapy (PDT) with gene therapy. We made use of a simple cationic guanidylated porphyrin (H2‑PG) with the hypothesis that porphyrin aggregates should be capable of complexing siRNA through multivalent interactions and thus contribute to its intracellular delivery, while remaining...
Source: International Journal of Pharmaceutics - August 1, 2019 Category: Drugs & Pharmacology Source Type: research

Methotrexate induced cell death mechanisms in MCF-7 adenocarcinoma breast cancer cells: Enhanced cytotoxicity following dff45-siRNA pre-treatment
Publication date: Available online 30 August 2018Source: SynergyAuthor(s): Fatemeh Kiani, Negin Rasouli, Tahereh Kashkoolinejad, Shahrokh Safarian, Seyed Jalal Zargar, Nader SheibaniAbstractThere are many efforts to diminish risks associated with the use of cancer chemotherapeutic agents through design and utilization of new strategies including gene therapy. We previously showed knockdown of dff45 or overexpression of dff40, the important apoptosis regulators, resulted in enhanced sensitization of cancer cells to chemotherapeutic agents. Here we show that pre-treatment with dff45-siRNA additively increased the overall cyt...
Source: Synergy - August 31, 2018 Category: Molecular Biology Source Type: research

Engineered Exosomes for Targeted Transfer of siRNA to HER2 Positive Breast Cancer Cells.
Abstract Exosomes are the best options for gene targeting, because of their natural, nontoxic, non-immunogenic, biodegradable, and targetable properties. By engineering exosome-producing cells, ligands can be expressed fusing with exosomal surface proteins for targeting cancer cell receptors. In the present study, HER2-positive breast cancer cells were targeted with a modified exosome producing engineered HEK293T cell. For this purpose, the HEK293T cells were transduced by a lentiviral vector bearing-LAMP2b-DARPin G3 chimeric gene. Stable cells expressing the fusion protein were selected, and the exosomes produced...
Source: Applied Biochemistry and Biotechnology - June 28, 2018 Category: Biochemistry Authors: Limoni SK, Moghadam MF, Moazzeni SM, Gomari H, Salimi F Tags: Appl Biochem Biotechnol Source Type: research