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Identification of a Novel 1,2 Oxazine That Can Induce Apoptosis by Targeting NF-κB in Hepatocellular Carcinoma Cells
Publication date: Available online 19 February 2020Source: Biotechnology ReportsAuthor(s): Chaithanya Somu, Chakrabhavi Dhananjaya Mohan, Sachin Ambedkar, Dukanya, Shobith Rangappa, CP Baburajeev, Alexey Sukhorukov, Srishti Mishra, Muthu K Shanmugam, Arunachalam Chinnathambi, Tahani Awad Alahmadi, Sulaiman Ali Alharbi, Basappa, Kanchugarakoppal S. RangappaAbstractConstitutive activation of NF-κB is associated with proinflammatory diseases and suppression of the NF-κB signaling pathway has been considered as an effective therapeutic strategy in the treatment of various cancers including hepatocellular carcinoma (HCC). Her...
Source: Biotechnology Reports - February 20, 2020 Category: Biotechnology Source Type: research

miR-324-5p inhibits gallbladder carcinoma cell metastatic behaviours by downregulation of transforming growth factor beta 2 expression.
Authors: Zhang X, Zhang L, Chen M, Liu D Abstract Increasing studies have demonstrated that microRNAs (miRNAs) are associated with the metastasis of gallbladder carcinoma (GBC). Recently, miR-324-5p has been reported to be a tumour-suppressive miRNA in many types of malignant cancer. However, the biological function and molecular mechanism of miR-324-5p in GBC still remain largely unknown. Here, we found that miR-324-5p expression was notably down-regulated in both GBC tissues and cells compared with that in normal controls. Downregulated miR-324-5p expression was negatively associated with the status of local inva...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 22, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Activatable MRI-monitoring gene delivery for the theranostic of renal carcinoma.
In this study, we constructed a PEG-MnO2-OPN siRNA nanocomplex via a modular streptavidin bridge for the theranostic treatment of renal carcinoma in vitro and in vivo. This strategy of utilizing PEG-MnO2 nanosheets for OPN siRNA delivery showed effective tumor growth inhibition of 786-O tumor-bearing mice, and such a process could be monitored by the activated T1-weight MRI, illustrating the promising potential of PEG-MnO2 nanosheets for the fabrication of an MRI-based theranostic system. PMID: 31734089 [PubMed - as supplied by publisher]
Source: Colloids and Surfaces - November 7, 2019 Category: Biotechnology Authors: Shi M, Wang S, Zheng S, Hou P, Dong L, He M, Wu C, Zhang X, Zuo F, Xu K, Li J Tags: Colloids Surf B Biointerfaces Source Type: research

Study of As2O3 regulating proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1.
CONCLUSION: As2O3 could regulate the proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1. PMID: 31079498 [PubMed - in process]
Source: Artificial Cells, Nanomedicine and Biotechnology - May 15, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Co-delivery of sorafenib and VEGF-siRNA via pH-sensitive liposomes for the synergistic treatment of hepatocellular carcinoma.
Authors: Yao Y, Wang T, Liu Y, Zhang N Abstract Non-viral nanocarrier affords a platform for drug and siRNA combination, the focus of which is to load drug and siRNA into a single carrier, allowing for co-delivery and a synergistic effect at tumour site. In our previous study, pH-sensitive carboxymethyl chitosan-modified liposomes (CMCS-SiSf-CL) were assembled for sorafenib (Sf) and Cy3-siRNA co-loaded. The present study evaluated in vitro and in vivo co-delivery of the co-loaded liposomes. Further, in vitro inhibiting hepatocellular carcinoma of the pH-sensitive sorafenib (Sf) and VEGF-siRNA co-loaded liposomes wa...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 14, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Co-delivery of sorafenib and VEGF-siRNA via pH-sensitive liposomes for the synergistic treatment of hepatocellular carcinoma
Volume 47, Issue 1, December 2019, Page 1374-1383 .
Source: Artificial Cells, Blood Substitutes, and Biotechnology - April 11, 2019 Category: Biotechnology Authors: Yao Yao Tianqi Wang Yongjun Liu Na Zhang Source Type: research

Activation of MET promotes resistance to sorafenib in hepatocellular carcinoma cells via the AKT/ERK1/2-EGR1 pathway.
In this study, we found that addition of HGF to sorafenib-treated HCC cells activated MET and re-stimulated the downstream AKT and ERK1/2 pathways. Thereby, restored sorafenib-treated HCC cells proliferation, migration and invasion ability, and rescued cells from apoptosis. In addition, we found that HGF treatment of HCC cells induced early growth response protein (EGR1) expression, which is involved in sorafenib resistance. Importantly, the HGF rescued effect in sorafenib-treated HCC cells could be abrogated by inhibiting MET activation with PHA-665752 or by downregulating EGR1 expression with small interfering RNA (siRNA...
Source: Artificial Cells, Nanomedicine and Biotechnology - January 23, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Liposome-mediated RNA interference delivery against Erk1 and Erk2 does not equally promote chemosensitivity in human hepatocellular carcinoma cell line HepG2.
CONCLUSION: Our data show that liposomal siRNA-mediated down-regulation of Erk1/2 can lead to potent chemosensitizing effects in HepG2 cells. Moreover, a higher chemosensitivity following Erk2 down-regulation than Erk1 down-regulation may be associated with the higher expression of Erk2 in human HCC. PMID: 28058860 [PubMed - as supplied by publisher]
Source: Artificial Cells, Nanomedicine and Biotechnology - January 7, 2017 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Polyelectrolyte complexes of hTERT siRNA and polyethyleneimine: Effect of degree of PEG grafting on biological and cellular activity.
Authors: Safari F, Tamaddon AM, Zarghami N, Abolmali S, Akbarzadeh A Abstract Gene silencing by siRNA (short interfering RNA)-targeted human telomerase reverse transcriptase (hTERT) is considered a successful strategy for cancer gene therapy. Polyelectrolyte complexes (PEC) of siRNA and cationic polymers such as polyethyleneimine (PEI) have been widely used for cellular transfection; however, they demonstrate some disadvantages such as cytotoxicity and extracellular matrix restrictions. PEG grafting technology was used in an attempt to improve the biocompatibility of PECs. Considering that this technology may compr...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 12, 2015 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

ΔNp63α-responsive microRNAs modulate the expression of metabolic enzymes in squamous cell carcinoma cells.
Abstract MicroRNAs, whose transcription is regulated by members of the tumor protein p53 family, modulate the expression of numerous metabolic enzymes, significantly altering tumor cell response to chemotherapeutic treatments. The role for ΔNp63α-regulated microRNAs in regulation of cell cycle arrest, apoptosis and autophagy in squamous cell carcinoma (SCC) cells upon cisplatin exposure has been reported. The current study indicated that the selected microRNA targets differentially regulated by ΔNp63α in cisplatin-sensitive and cisplatin-resistant SCC cells could alter the expression of a few metabolic enzymes...
Source: Current Pharmaceutical Biotechnology - June 19, 2015 Category: Biotechnology Authors: Ratovitski EA Tags: Curr Pharm Biotechnol Source Type: research

Tumor suppressor protein p53 promotes 2-methoxyestradiol-induced activation of Bak and Bax, leading to mitochondria-dependent apoptosis in human colon cancer HCT116 cells.
Abstract To examine the effect of tumor suppressor protein p53 on the antitumor activity of 2-methoxyestradiol (2-MeO-E2), 2-MeO-E2-induced cell cycle changes and apoptotic events were compared between human colon carcinoma cell lines HCT116 (p53+/+) and HCT116 (p53-/-). When both cell types were exposed to 2-MeO-E2, a reduction in the cell viability and an enhancement in the proportions of G2/M cells and apoptotic sub-G1 cells commonly occurred dose-dependently. These 2-MeO-E2-induced cellular changes, except for G2/M arrest, appeared to be more apparent in the presence of p53. Immunofluorescence microscopic anal...
Source: Journal of Microbiology and Biotechnology - September 1, 2014 Category: Biotechnology Authors: Lee JY, Lee SB, Park WY, Choi YJ, Kim B, Kim YH, Jun DY, Kim YH Tags: J Microbiol Biotechnol Source Type: research

miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2.
Abstract MicroRNAs play important roles in carcinogenesis by negatively regulating the expression of target genes. Here we explore the biological function of miR-155 and the underlying mechanism in colorectal carcinoma. We validate, for the first time, that E2F2 is a direct target of miR-155 using western blot and a luciferase reporter assay and that miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2 using siRNA technology. We also found, for the first, time that E2F2 acts as a tumor suppressor in colorectal carcinoma. Overall, miR-155 plays an important role in colo...
Source: Biotechnology Letters - May 4, 2014 Category: Biotechnology Authors: Li T, Yang J, Lv X, Liu K, Gao C, Xing Y, Xi T Tags: Biotechnol Lett Source Type: research