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Effects of chitosan nanoparticle-mediated BRAF siRNA interference on invasion and metastasis of gastric cancer cells
Volume 44, Issue 5, August 2016, pages 1232-123510.3109/21691401.2015.1019666Jian Huo
Source: Artificial Cells, Blood Substitutes, and Biotechnology - March 20, 2015 Category: Biotechnology Authors: Jian Huo Source Type: research

Knockdown of STAT3 expression in SKOV3 cells by biodegradable siRNA-PLGA/CSO conjugate micelles.
Abstract Biodegradable and biocompatible poly(d,l-lactic-co-glycolic acid) (PLGA)was conjugated to the 5'-thiol end of signal transducer and activator of transcription 3 (STAT3) small interfering RNA (STAT3-siRNA) via a disulfide bond. In aqueous environments, these siRNA-PLGA conjugates can spontaneously form core/shell type spherical micelles with a particle size of about 200nm. A biodegradable, low molecular weight cationic polymer, chitosan oligosaccharide (CSO), was added to the siRNA-PLGA micelles at different nitrogen to phosphate (N/P) ratios to form stable, spherical siRNA-PLGA/CSO micelles with sizes of ...
Source: Colloids and Surfaces - January 28, 2015 Category: Biotechnology Authors: Zhao Y, Zheng C, Zhang L, Chen Y, Ye Y, Zhao M Tags: Colloids Surf B Biointerfaces Source Type: research

siRNAs encapsulated in Recombinant Capsid protein derived from Dengue serotype 2 virus inhibits the four serotypes of the virus and proliferation of cancer cells
In this study we have evaluated the utility of the protein as an siRNA carrier for anti Dengue viral and anti cancer applications using cell culture systems. We show that target specific siRNAs delivered using the capsid protein inhibit infection by the four serotypes of Dengue virus and proliferation of two cancer cell lines. Our data confirm the potential of the capsid for anti Dengue viral and anti cancer RNAi applications. In addition, we have optimized a fermentation strategy to improve the yield of E. coli expressed D2C protein since the reported yield of E.coli expressed flaviviral capsid proteins are low.
Source: Journal of Biotechnology - November 13, 2014 Category: Biotechnology Source Type: research

Tumor suppressor protein p53 promotes 2-methoxyestradiol-induced activation of Bak and Bax, leading to mitochondria-dependent apoptosis in human colon cancer HCT116 cells.
Abstract To examine the effect of tumor suppressor protein p53 on the antitumor activity of 2-methoxyestradiol (2-MeO-E2), 2-MeO-E2-induced cell cycle changes and apoptotic events were compared between human colon carcinoma cell lines HCT116 (p53+/+) and HCT116 (p53-/-). When both cell types were exposed to 2-MeO-E2, a reduction in the cell viability and an enhancement in the proportions of G2/M cells and apoptotic sub-G1 cells commonly occurred dose-dependently. These 2-MeO-E2-induced cellular changes, except for G2/M arrest, appeared to be more apparent in the presence of p53. Immunofluorescence microscopic anal...
Source: Journal of Microbiology and Biotechnology - September 1, 2014 Category: Biotechnology Authors: Lee JY, Lee SB, Park WY, Choi YJ, Kim B, Kim YH, Jun DY, Kim YH Tags: J Microbiol Biotechnol Source Type: research

Gene silencing effect of SiRNA-magnetic modified with biodegradable copolymer nanoparticles on hTERT gene expression in lung cancer cell line
Artificial Cells, Nanomedicine, and Biotechnology, Ahead of Print.
Source: Artificial Cells, Blood Substitutes, and Biotechnology - July 14, 2014 Category: Biotechnology Tags: article Source Type: research

Development of Two-Component Nanorod Complex for Dual-Fluorescence Imaging and siRNA delivery.
This study demonstrates the feasibility of using two-component nanorods as a potential theranostic in breast cancer treatment with capabilities in dual imaging and targeted gene delivery. PMID: 24986677 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - July 2, 2014 Category: Biotechnology Authors: Choi JH, Oh BK Tags: J Microbiol Biotechnol Source Type: research

Nano Carriers That Enable Co-Delivery of Chemotherapy and RNAi Agents for Treatment of Drug-Resistant Cancers.
Abstract Tumor cells exhibit drug resistant phenotypes that decrease the efficacy of chemotherapeutic treatments. The drug resistance has a genetic basis that is caused by an abnormal gene expression. There are several types of drug resistance: efflux pumps reducing the cellular concentration of the drug, alterations in membrane lipids that reduce cellular uptake, increased or altered drug targets, metabolic alteration of the drug, inhibition of apoptosis, repair of the damaged DNA, and alteration of the cell cycle checkpoints (Gottesman et al. , 2002, Holohan et al. , 2013). siRNA is used to silence the drug resi...
Source: Biotechnology Advances - June 9, 2014 Category: Biotechnology Authors: Tsouris V, Joo MK, Kim SH, Kwon IC, Won YY Tags: Biotechnol Adv Source Type: research

Knockdown of ribosomal protein L39 by RNA interference inhibits the growth of human pancreatic cancer cells in vitro and in vivo.
Abstract Pancreatic cancer remains a major unsolved health problem lacking a potent therapeutic option. Our previous studies showed that the ribosomal protein L39 (RPL39) gene was up-regulated after long-term silencing of oncogenic KRAS in pancreatic cancer PANC-1 cells, which indicated that RPL39 may be important for pancreatic cancer development and survival. In the current study, small interfering RNA (siRNA) targeting of the RPL39 gene was performed to determine the effects of the RPL39 gene on growth of pancreatic cancer PANC-1 and BxPC-3 cells in vitro and in vivo. Results from in vitro experiments showed th...
Source: Biotechnology Journal - May 1, 2014 Category: Biotechnology Authors: Li CO, Chen D, Luo M, Ge M, Zhu J Tags: Biotechnol J Source Type: research

Tristetraprolin regulates prostate cancer cell growth through suppression of E2F1.
Abstract The transcription factor E2F1 is active during the G1 to S transition and is involved in cell cycle and progression. A recent study reported that increased E2F1 is associated with DNA damage and tumour development in several tissues using transgenic models. Here, we show that E2F1 expression is regulated by tristetraprolin (TTP) in prostate cancer. Overexpression of TTP decreased the stability of E2F1 mRNA and the expression level of E2F1. In contrast, inhibition of TTP using siRNA increased the E2F1 expression. E2F1 mRNA contains three AREs within the 3'UTR and TTP destabilized a luciferase mRNA that con...
Source: Journal of Microbiology and Biotechnology - October 22, 2013 Category: Biotechnology Authors: Lee HH, Lee SR, Leem SH Tags: J Microbiol Biotechnol Source Type: research

Rigid nanoparticle-based delivery of anti-cancer siRNA: Challenges and opportunities.
Abstract Gene therapy is a promising strategy to treat various genetic and acquired diseases. Small interfering RNA (siRNA) is a revolutionary tool for gene therapy and the analysis of gene function. However, the development of a safe, efficient, and targetable non-viral siRNA delivery system remains a major challenge in gene therapy. An ideal delivery system should be able to encapsulate and protect the siRNA cargo from serum proteins, exhibit target tissue and cell specificity, penetrate the cell membrane, and release its cargo in the desired intracellular compartment. Nanomedicine has the potential to deal with...
Source: Biotechnology Advances - September 4, 2013 Category: Biotechnology Authors: Wang Z, Liu G, Zheng H, Chen X Tags: Biotechnol Adv Source Type: research

Ultrastable pRNA hexameric ring gearing hexameric phi29 DNA-packaging motor by revolving without rotating and coiling.
Abstract Biomotors have previously been classified into two categories: linear and rotational motors. It has long been popularly believed that viral DNA packaging motors are rotation motors. We have recently found that the DNA-packaging motor of bacteriophage phi29 uses a third mechanism: revolution without rotation. phi29 motor consists of three-coaxial rings of hexameric RNA, a hexameric ATPase, and a dodecameric channel. The motor uses six ATP to revolve one helical turn of dsDNA around the hexameric ring of ATPase gp16. Each dodecameric segment tilts at a 30°-angle and runs anti-parallel to the dsDNA helix to...
Source: Current Opinion in Biotechnology - May 14, 2013 Category: Biotechnology Authors: Schwartz C, Guo P Tags: Curr Opin Biotechnol Source Type: research

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