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SIRT3 Promotes the Development of Esophageal Squamous Cell Carcinoma by Regulating Hexokinase 2 through the AKT Signaling Pathway
In the present study, we explored whether sirtuin-3 (SIRT3) regulates the proliferation and migration of esophageal squamous cell carcinoma (ESCC) and investigated the mechanisms underlying the oncogene role of SIRT3. siRNA was used to transfect Eca109 cells and downregulate SIRT3. The proliferation and migration of Eca109 cells were examined by the CCK-8 assay, colony formation assay, Transwell assay, and scratch test. Quantitative real-time PCR and Western blotting were used to detect SIRT3, hexokinase 2, AKT, and p-AKT in Eca109 cells. Functional assays showed that downregulation of SIRT3 could inhibit the proliferation...
Source: Bulletin of Experimental Biology and Medicine - November 28, 2022 Category: Biology Source Type: research

Ku80 promotes melanoma growth and regulates antitumor effect of melatonin by targeting HIF1-α dependent PDK-1 signaling pathway
In this study, we screened a siRNA library targeting 6024 human genes and identified Ku80 as a potential therapeutic target in melanoma cells. Knockdown of Ku80 significantly suppressed melanoma cell proliferation and induced apoptosis, as well as enhanced the antitumor effect of melatonin in melanoma in vitro and in vivo. Overexpression of Ku80, however, promoted melanoma growth and increased the insensitivity of melanoma cells to melatonin. Mechanistically, we found that Ku80 bound to the PDK1 promoter and activated the transcription of PDK1. Moreover, we showed that the binding of Ku80 at the PDK-1 promoter was HIF1-α ...
Source: Redox Biology - April 22, 2019 Category: Biology Source Type: research

Knockdown of Delta-like 3 restricts lipopolysaccharide-induced inflammation, migration and invasion of A2058 melanoma cells via blocking Twist1-mediated epithelial-mesenchymal transition
Publication date: Available online 11 April 2019Source: Life SciencesAuthor(s): Xiaojie Ding, Fuyao Li, Li ZhangAbstractAimsTo investigate the effects and mechanisms of DLL3 in inflammation-mediated A2058 melanoma cell invasion and metastasis.Materials and methodsMelanoma A2058 cells was stimulated with lipopolysaccharide (LPS), with or without transfection of DLL3 siRNA, or DLL3 overexpression vector, or Twist1 siRNA. Cell migration and invasion were detected by wound healing and transwell invasion assay. The production of inflammatory factors TNF-α and IL-6 was measured by ELISA. The expression of Notch signaling-relate...
Source: Life Sciences - April 13, 2019 Category: Biology Source Type: research

AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model
Publication date: Available online 18 February 2019Source: Life SciencesAuthor(s): Xuyang Wang, Jinyu Pan, Hui Liu, Mingjun Zhang, Dian Liu, Lu Lu, Jingjing Tian, Ming Liu, Tao Jin, Fengshuang AnAbstractAimsAbsent in melanoma 2 (AIM2) is a cytosolic DNA sensor which plays an important role in inflammasome formation and is involved in various cellular functions including pyroptosis, fibrosis, and tissue injury. Our study aimed to investigate whether AIM2 plays a role in diabetic cardiomyopathy (DCM) and to explore its potential molecular mechanism.Main methodsSprague-Dawley rats were randomly divided into 4 groups: Control,...
Source: Life Sciences - February 18, 2019 Category: Biology Source Type: research

Honokiol induces endoplasmic reticulum stress-mediated apoptosis in human lung cancer cells
Publication date: Available online 29 January 2019Source: Life SciencesAuthor(s): Jianfei Zhu, Shuonan Xu, Wenli Gao, Jianyu Feng, Guolong ZhaoAbstractAimsHonokiol is a hydroxylated biphenyl natural product and displays potent antitumor activity against several cancers including prostate cancer, melanoma, leukemia, and colorectal cancer. The present study was to investigate the in vitro activity of honokiol against A549 and 95-D human lung cancer cells.Main methodsA549 and 95-D cells were used with honokiol treatment. Cell viability was determined by CCK-8 assay. The cell migration and apoptosis were evaluated by wound hea...
Source: Life Sciences - January 29, 2019 Category: Biology Source Type: research

Srb1 as a new redox target of cigarette smoke in human sebocytes.
In conclusion, exposure to CS, induced SRB1 post-translational modifications in sebocytes and this might affect sebocytes/skin functionality. PMID: 27865981 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - November 15, 2016 Category: Biology Authors: Crivellari I, Sticozzi C, Belmonte G, Muresan XM, Cervellati F, Pecorelli A, Cavicchio C, Maioli E, Zouboulis CC, Benedusi M, Cervellati C, Valacchi G Tags: Free Radic Biol Med Source Type: research

Association of Notch4 with metastasis in human oral squamous cell carcinoma
This study therefore investigates (1) the expression of Notch4 and its downstream target myelin associated glycoprotein (MAG) in tissue samples representative of different stages of OSCC with varied clinicopathological features and (2) the possible involvement of Notch4 in the proliferation and migration of OSCC cells. Main methods Sixty patients reported positive for OSCC were obtained along with the clinicopathological parameters and we performed immunohistochemistry, western blotting and RT-PCR for Notch4 and MAG expression. Further, the metastatic role of Notch4 was analyzed in the HSC-3 cell line by cell proliferatio...
Source: Life Sciences - May 16, 2016 Category: Biology Source Type: research

Melanoma upregulates ICAM-1 expression on endothelial cells through engagement of tumor CD44 with endothelial E-selectin and activation of a PKC{alpha}-p38-SP-1 pathway Research Communications
In conclusion, CD44/selectin binding signals ICAM-1 up-regulation on the EC surface through a PKCα–p38–SP-1 pathway, which further enhances melanoma cell adhesion to ECs during metastasis.—Zhang, P., Goodrich, C., Fu, C., Dong, C. Melanoma upregulates ICAM-1 expression on ECs through engagement of tumor CD44 with endothelial E-selectin and activation of a PKCα–p38–SP-1 pathway.
Source: FASEB Journal - October 30, 2014 Category: Biology Authors: Zhang, P., Goodrich, C., Fu, C., Dong, C. Tags: Research Communications Source Type: research

Proopiomelanocortin gene delivery induces apoptosis in melanoma through NADPH oxidase 4-mediated ROS generation.
In this study, we characterize the involvement of hypoxia and reactive oxygen species (ROS) generation in the antineoplastic mechanism of proopiomelanocortin (POMC) gene delivery in a mouse B16-F10 melanoma model in vivo and in vitro. Histological analysis revealed increased TUNEL-positive cells and enhanced hypoxic activities in melanoma treated with adenovirus encoding POMC (Ad-POMC) but not control vector. Because the apoptotic cells were detected mainly in regions distant from blood vessels, it was hypothesized that POMC therapy might render melanoma cells vulnerable to hypoxic insult. Using a hypoxic chamber or cobalt...
Source: Free Radical Biology and Medicine - January 8, 2014 Category: Biology Authors: Liu GS, Wu JC, Tsai HE, Dusting GJ, Chan EC, Wu CS, Tai MH Tags: Free Radic Biol Med Source Type: research

The EJC component Magoh regulates proliferation and expansion of neural crest-derived melanocytes.
Abstract Melanoblasts are a population of neural crest-derived cells that generate the pigment-producing cells of our body. Defective melanoblast development and function underlies many disorders including Waardenburg syndrome and melanoma. Understanding the genetic regulation of melanoblast development will help elucidate the etiology of these and other neurocristopathies. Here we demonstrate that Magoh, a component of the exon junction complex, is required for normal melanoblast development. Magoh haploinsufficient mice are hypopigmented and exhibit robust genetic interactions with the transcription factor, Sox1...
Source: Developmental Biology - January 17, 2013 Category: Biology Authors: Silver DL, Leeds KE, Hwang HW, Miller EE, Pavan WJ Tags: Dev Biol Source Type: research