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Specialty: Orthopaedics

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Total 95 results found since Jan 2013.

Improved neurite outgrowth on central nervous system myelin substrate by siRNA-mediated knockdown of Nogo receptor.
CONCLUSION: siRNA-mediated knockdown of NgR expression contributes to neurite outgrowth in vitro. PMID: 27033267 [PubMed - in process]
Source: Chinese Journal of Traumatology - January 31, 2016 Category: Orthopaedics Authors: Ding SH, Bao YH, Shen JH, Gao GY, Pan YH, Luo QZ, Jiang JY Tags: Chin J Traumatol Source Type: research

Small Players Ruling the Hard Game: siRNA in Bone Regeneration
This article is protected by copyright. All rights reserved
Source: Journal of Bone and Mineral Research - February 1, 2016 Category: Orthopaedics Authors: S Ghadakzadeh, M Mekhail, A Aoude, R Hamdy, M Tabrizian Tags: Review Source Type: research

Effective knock down of matrix metalloproteinase‐13 by an intra‐articular injection of small interfering RNA (siRNA) in a murine surgically‐induced osteoarthritis model
This study investigated the effect of MMP‐13 gene knock down on cartilage degradation by injecting small interfering RNA (siRNA) into knee joints in a mouse model of osteoarthritis (OA). OA was induced in male C57BL/6 mice by destabilization of medial meniscus (DMM) surgery. Change of Mmp13 expression over time was determined by qPCR analysis from 3 days to 6 weeks after surgery. Mmp13 and control chemically modified siRNA were injected into the knee joint 1 week after surgery and expression levels were assessed in synovium by qPCR 48 h later. Cartilage degradation was histologically assessed 8 weeks after DMM surgery ...
Source: Journal of Orthopaedic Research - May 23, 2014 Category: Orthopaedics Authors: Ryuichiro Akagi, Takahisa Sasho, Masahiko Saito, Jun Endo, Satoshi Yamaguchi, Yuta Muramatsu, Shunsuke Mukoyama, Yorikazu Akatsu, Joe Katsuragi, Taisuke Fukawa, Kazuhisa Takahashi Tags: Research Article Source Type: research

Blockage of Src by Specific siRNA as a Novel Therapeutic Strategy to Prevent Destructive Repair in Steroid‐Associated Osteonecrosis in Rabbits
This study was designed to prove our hypothesis that blocking VEGF‐Src signaling pathway by specific Src siRNA was able to prevent destructive repair in a SAON rabbit model. Destructive repair in SAON was induced in rabbits. At 2, 4, 6 weeks after SAON induction, VEGF, anti‐VEGF, Src siRNA, Src siRNA + VEGF, control siRNA and saline were intramedullary injected into proximal femora for each group, respectively. Vascularization and permeability were quantified by dynamic contrast‐enhanced (DCE) MRI. At week 6 after SAON induction, proximal femora were dissected for micro‐CT‐based trabecular architecture with f...
Source: Journal of Bone and Mineral Research - April 27, 2015 Category: Orthopaedics Authors: Li‐zhen Zheng, Hui‐juan Cao, Shi‐hui Chen, Tao Tang, Wei‐min Fu, Le Huang, Dick Ho Kiu Chow, Yi‐xiang Wang, James Francis Griffith, Wei He, Hong Zhou, De‐wei Zhao, Ge Zhang, Xin‐luan Wang, Ling Qin Tags: Original Article Source Type: research

Cationic Nanogel-mediated Runx2 and Osterix siRNA Delivery Decreases Mineralization in MC3T3 Cells.
CONCLUSIONS: Although mRNA and protein knockdown were confirmed as a result of RNAi treatments against Runx2 and Osx, complete elimination of mineralization processes was not achieved. RNAi targeting mid- and late-stage osteoblast differentiation markers such as ALP, osteocalcin, osteopontin, and bone sialoprotein) may produce the desired RNAi-nanogel nanostructured polymer HO prophylaxis. CLINICAL RELEVANCE: Successful HO prophylaxis should target and silence osteogenic markers critical for heterotopic bone formation processes. The identification of such markers, beyond RUNX2 and OSX, may enhance the effectiveness of...
Source: Clinical Orthopaedics and Related Research - December 2, 2014 Category: Orthopaedics Authors: Shrivats AR, Hsu E, Averick S, Klimak M, Watt AC, DeMaio M, Matyjaszewski K, Hollinger JO Tags: Clin Orthop Relat Res Source Type: research

Src siRNA prevents corticosteroid-associated osteoporosis in a rabbit model
In an established steroid-associated osteonecrosis (SAON) rabbit model we found recently that blockage Src by siRNA could improve reconstructive repair of osteonecrosis via enhancing osteogenesis and inhibiting bone resorption. The current study investigated if blocking Src was able to prevent steroid-associated osteoporosis (SAOP) in the same SAON animal model. Rabbits were treated with pulsed lipopolysaccharide (LPS) and corticosteroid methylprednisolone (MPS). At 2, 4, 6weeks after induction, Src siRNA, control siRNA and saline were intramedullary injected into proximal femur, respectively.
Source: Bone - November 17, 2015 Category: Orthopaedics Authors: Li-Zhen Zheng, Xin-Luan Wang, Hui-Juan Cao, Shi-Hui Chen, Le Huang, Ling Qin Tags: Original Full Length Article Source Type: research

Small Interfering RNA-Mediated Suppression of Fas Modulate Apoptosis and Proliferation in Rat Intervertebral Disc Cells.
Conclusions: The observed dual positive effect of Fas siRNA might be a powerful therapeutic approach for disc degeneration by suppression of harmful gene expression. PMID: 29093776 [PubMed]
Source: Asian Spine Journal - November 3, 2017 Category: Orthopaedics Tags: Asian Spine J Source Type: research

Effect of RNA Interference-Mediated Suppression of p75 on the Viability of Rat Notochordal Cells.
CONCLUSIONS: siRNA-mediated suppression of p75 inhibited apoptosis and increased proliferation of notochordal cells under conditions of serum deprivation, suggesting that RNAi might serve as a novel therapeutic approach for disc degeneration caused by insufficient viability of disc cells through the suppression of the expression of harmful genes. PMID: 27994772 [PubMed]
Source: Asian Spine Journal - December 21, 2016 Category: Orthopaedics Tags: Asian Spine J Source Type: research

The effect of noggin interference in a rabbit posterolateral spinal fusion model
Conclusions Early noggin suppression does not appear to enhance the BMP activity sufficiently to significantly affect final fusion rates in our model.
Source: European Spine Journal - October 30, 2014 Category: Orthopaedics Source Type: research

PEG10 counteracts signaling pathways of TGF- β and BMP to regulate growth, motility and invasion of SW1353 chondrosarcoma cells
AbstractRecently, we reported highly active transforming growth factor (TGF)- β and bone morphogenetic protein (BMP) signaling in human chondrosarcoma samples and concurrent downregulation of paternally expressed gene 10 (PEG10). PEG10 expression was suppressed by TGF- β signaling, and PEG10 interfered with the TGF-β and BMP-SMAD pathways in chondrosarcoma cells. However, the roles of PEG10 in bone tumors, including chondrosarcoma, remain unknown. Here, we report that PEG10 promotes SW1353 chondrosarcoma cell growth by preventing TGF-β1-mediated suppression. In contrast, PEG10 knockdown augments the TGF-β1-induced mot...
Source: Journal of Bone and Mineral Metabolism - August 9, 2018 Category: Orthopaedics Source Type: research

Modulators of Fam210a and Roles of Fam210a in the Function of Myoblasts
In conclusion, Fam210a might enhance myoblast differentiation and proteolysis. Moreover, insulin and 1,25(OH)2D may induce myoblast differentiation and degradation by enhancing the expression of Fam210a.
Source: Calcified Tissue International - January 23, 2020 Category: Orthopaedics Source Type: research

Evaluation of the Effect of (S)-3,4-Dicarboxyphenylglycine as a Metabotropic Glutamate Receptors Subtype 8 Agonist on Thermal Nociception Following Central Neuropathic Pain.
Conclusions: The results revealed that activation of mGluR8 in PAG is not capable of improving the thermal hyperalgesia threshold. Based on the decreased expression of mGluR8 after SCI induced by clip compression injury and its significant increase after treatment of siRNA against mGluR8, this method might still hold promise as an effective treatment of neuropathic pain. It can be concluded that increased expression of mGluR8 is due to the fact that DCPG prevents the death of neurons that express these receptors. PMID: 32460469 [PubMed - as supplied by publisher]
Source: Asian Spine Journal - May 29, 2020 Category: Orthopaedics Tags: Asian Spine J Source Type: research

LncPVT1 promotes cartilage degradation in diabetic OA mice by downregulating miR-146a and activating TGF- β/SMAD4 signaling
ConclusionsOur results demonstrate LncRNA PVT1 is involved in cartilage degradation in diabetic OA and correlated with disease severity. Efficiency of ad-siRNA-PVT1 in controlling joint inflammation in diabetic OA mice is associated with the suppression of the expression of miR-146a, pro-inflammatory cytokines and activation of TGF- β/SMAD4 pathway.
Source: Journal of Bone and Mineral Metabolism - February 10, 2021 Category: Orthopaedics Source Type: research

Role of the parathyroid hormone type 1 receptor (PTH1R) as a mechanosensor in osteocyte survival
This article is protected by copyright. All rights reserved
Source: Journal of Bone and Mineral Research - December 1, 2014 Category: Orthopaedics Authors: Marta Maycas, Juan A. Ardura, Luis Fernández de Castro, Beatriz Bravo, Arancha R. Gortázar, Pedro Esbrit Tags: Original Article Source Type: research

Expression of Caveolin-1 in Periodontal Tissue and Its Role in Osteoblastic and Cementoblastic Differentiation In Vitro
This study demonstrates, for the first time, that Cav-1 is expressed in developing periodontal tissue and in vitro in periodontal-related cells. Cav-1 inhibition positively regulates osteoblastic differentiation in hPDLCs, cementoblasts, and osteoblasts via BMP, AMPK, MAPK, and NF-κB pathway. Thus, Cav-1 inhibition may be a novel molecular target for therapeutic approaches in periodontitis or osteolytic disease.
Source: Calcified Tissue International - December 19, 2015 Category: Orthopaedics Source Type: research