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Specialty: Drugs & Pharmacology

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Total 3220 results found since Jan 2013.

Cytoplasmic Delivery of Functional siRNA Using pH-Responsive Nanoscale Hydrogels
Publication date: Available online 8 March 2019Source: International Journal of PharmaceuticsAuthor(s): William B. Liechty, Rebekah L. Scheuerle, Julia E. Vela Ramirez, Nicholas A. PeppasAbstractThe progress of short interfering RNA (siRNA) technologies has unlocked the development of novel alternatives for the treatment of a myriad of diseases, including viral infections, autoimmune disorders, or cancer. Nevertheless, the clinical use of these therapies faces significant challenges, mainly overcoming the charged and large nature of these molecules to effectively enter the cell. In this work, we developed a cationic polyme...
Source: International Journal of Pharmaceutics - March 9, 2019 Category: Drugs & Pharmacology Source Type: research

Targeted Cancer Treatment Using a Combination of siRNA-Liposomes and Resveratrol-Electrospun Fibers in Co-Cultures
In this study, we evaluated a novel combination of drug delivery devices composed of holo-transferrin conjugated liposomes for siRNA (36 nM) delivery, and electrospun polycaprolactone (PCL)-gelatin (GT) microfibers for resveratrol (40 µM) release. Single- and co-cultures of cancerous K562 cells and human umbilical vein endothelial cells (HUVECs) were used to test the efficacy and targeting over eight days. BCR-ABL siRNA-encapsulated (36 nM) holo-transferrin-conjugated PEG-liposomes were characterized using dynamic light scattering, and transmission electron microscopy. RT-qPCR was performed to assess the silencing BCR-ABL...
Source: International Journal of Pharmaceutics - August 6, 2019 Category: Drugs & Pharmacology Source Type: research

The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells
In this study, a pH-responsive micelle based on polycaprolactone-block–poly 2-(dimethylamino)ethyl methacrylate (PCL-PDEM) cationic copolymer was developed to carry short interfering RNA (siRNA) for silencing interleukin 8 (IL-8) gene in hepatoma cancer cells. The transfection efficiency of the PCL-PDEM-siRNA/quantum dots (QDs) nanoplex has reached about 70%, and the expression level of IL-8 decreased about 63%. Furthermore, the codelivery of QDs and siRNA has been realized, which is beneficial to visualize the process of siRNA delivery. No considerable cytotoxicity from the nanoparticles has been observed, indicating th...
Source: Frontiers in Pharmacology - October 9, 2019 Category: Drugs & Pharmacology Source Type: research

Exploring Sirna Umpired Nanogels: A Tale Of Barrier Combating Carrier.
Abstract Potential short interfering RNAs (siRNA) modulating gene expression are emerged as a novel therapeutic arsenal against a wide range of maladies and disorders containing cancer, viral infections, bacterial ailments and metabolic snags at molecular level. Nanogel in current medicinal era displayed a comprehensive range of significant drug delivery prospectives. Biodegradation, swelling and de-swelling tendency, pH sensitive drug release, thermo-sensitivity are some of the renowned associated benefits of nanogel drug delivery system. Global researches have also showed that nanogel system significantly target...
Source: Current Pharmaceutical Design - April 16, 2020 Category: Drugs & Pharmacology Authors: Kashaw SK, Sahu P, Rajoriya V, Jana P, Kashaw V, Sau S, Iyer AK Tags: Curr Pharm Des Source Type: research

Mirror siRNAs loading for dual delivery of doxorubicin and autophagy regulation siRNA for multidrug reversing chemotherapy.
Abstract The multidrug resistance (MDR) which widely observed in multiple cancer types is responsible for the poor chemotherapy benefits of doxorubicin (Dox). Here in our study, Dox was firstly loaded into a scramble siRNA and then condensed by polyethyleneimine (PEI) 25k together with anti-autophagy siRNA, the obtained PEI/Si-D containing mirror RNAs was further coated with hyaluronic acid (HA) to shield the surface charge of PEI and offer tumor-homing property that finally developed a platform for effective cancer chemotherapy (HP/Si-D). Our results revealed that the obtained HP/Si-D was showed high stability an...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - July 22, 2020 Category: Drugs & Pharmacology Authors: Yang B, Hao A, Chen L Tags: Biomed Pharmacother Source Type: research

Prolonged cultured human hepatocytes as an in vitro experimental system for the evaluation of potency and duration of activity of RNA therapeutics: Demonstration of prolonged duration of gene silencing effects of a GalNAc-conjugated human hypoxanthine phosphoribosyl transferase (HPRT1) siRNA.
We report here the evaluation of a novel in vitro experimental model, prolonged cultured human hepatocytes (PCHC), as an experimental system to evaluate the potency and duration of effects of oligonucleotide therapeutics. A novel observation was made on the redifferentiation of PCHC upon prolonged culturing based on mRNA profiling of characteristic hepatic differentiation marker genes albumin, transferrin, and transthyretin. Consistent with the known de-differentiation of cultured human hepatocytes, decreases in marker gene expression were observed upon culturing of the hepatocytes for 2 days. A novel observation of re-dif...
Source: Biochemical Pharmacology - December 21, 2020 Category: Drugs & Pharmacology Authors: Yang Q, Humphreys SC, Lade JM, Li AP Tags: Biochem Pharmacol Source Type: research

cRGD peptide-conjugated polyethylenimine-based lipid nanoparticle for intracellular delivery of siRNA in hepatocarcinoma therapy
Drug Deliv. 2021 Dec;28(1):995-1006. doi: 10.1080/10717544.2021.1928794.ABSTRACTThe effective delivery system plays an important role in the application of siRNA in the antitumor study. However, until now, researches on the delivery systems targeting hepatocarcinoma cells are still being explored. Here we designed and prepared a novel siRNA delivery system, cRGD-PSH-NP, which was based on a modified polyethyleneimine (PSH) and DSPE-PEG2000-cRGD. cRGD-PSH-NP loaded with survivin siRNA (cRGD-PSH-NP/S) was composed of egg phosphatidylcholine, cationic PSH, PEGylated lipids, survivin siRNA, and cRGD peptide as a targeting liga...
Source: Drug Delivery - May 27, 2021 Category: Drugs & Pharmacology Authors: Shuang Yang Dandan Wang Xia Zhang Yaojun Sun Bin Zheng Source Type: research

Development of siRNA Delivery System by Lipid Nanoparticles Modified with Functional Materials for Cancer Treatment
Biol Pharm Bull. 2022;45(8):972-977. doi: 10.1248/bpb.b22-00317.ABSTRACTNucleic acid drugs can control gene expression and function in a manner different from that of conventional compounds. On the other hand, nucleic acids can be easily degraded in the in vivo circumstances. In addition, nucleic acids cannot penetrate cell membranes. Therefore, a drug delivery system (DDS) is essential to protect nucleic acid molecules until they reach the target cell and to release them efficiently inside the cell. In order to apply nucleic acid drugs to new cancer therapeutic strategies, the author has been developing a DDS that enables...
Source: Biological and Pharmaceutical Bulletin - July 31, 2022 Category: Drugs & Pharmacology Authors: Yu Sakurai Source Type: research