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Infectious Disease: Cytomegalovirus

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Total 27 results found since Jan 2013.

Human cytomegalovirus triggers the assembly of AIM2 inflammasome in THP ‐1‐derived macrophages
In conclusion, HCMV infection induces an AIM2 inflammasome response, which negatively influences viral life cycle. This article is protected by copyright. All rights reserved
Source: Journal of Medical Virology - May 8, 2017 Category: Virology Authors: Yuan Huang, Lingling Liu, Di Ma, Yi Liao, Yuanyuan Lu, Heyu Huang, Wenqing Qin, Xinglou Liu, Feng Fang Tags: Research Article Source Type: research

Human cytomegalovirus may promote tumour progression by upregulating arginase-2.
CONCLUSIONS: ARG2 promotes tumorigenesis, and HCMV may contribute to GBM pathogenesis by upregulating ARG2. PMID: 27363017 [PubMed - as supplied by publisher]
Source: Oncotarget - July 2, 2016 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

The viral G protein-coupled receptor ORF74 unmasks phospholipase C signaling of the receptor tyrosine kinase IGF-1R.
In this study we examined the effect of the constitutively active viral ORF74 on human IGF-1R signaling. Constitutive and CXCL1-induced ORF74 signaling did not transactivate IGF-1R. In contrast, IGF-1 stimulated phospholipase C (PLC) activation in an ORF74-dependent manner without affecting chemokine binding to ORF74. Inhibition of constitutive ORF74 activity by mutagenesis or the inverse agonist CXCL10, or neutralizing IGF-1R with an antibody or silencing IGF-1R expression using siRNA inhibited PLC activation by IGF-1. Transactivation of ORF74 in response to IGF-1 occurred independently of Src, PI3K, and secreted ORF74 li...
Source: Cellular Signalling - February 27, 2016 Category: Cytology Authors: de Munnik SM, van der Lee R, Velders DM, van Offenbeek J, Smits-de Vries L, Leurs R, Smit MJ, Vischer HF Tags: Cell Signal Source Type: research

Epstein-Barr virus glycoprotein gM can interact with the cellular protein p32 and knockdown of p32 impairs virus.
We report here that the long predicted cytoplasmic tail of gM is not required for complex formation and that it interacts with the cellular protein p32, which has been reported to be involved in nuclear egress of human cytomegalovirus and herpes simplex virus. Although redistribution of p32 and colocalization with gM was not observed in virus infected cells, knockdown of p32 expression by siRNA or lentivirus-delivered shRNA recapitulated the phenotype of a virus lacking expression of gNgM. A proportion of virus released from cells sedimented with characteristics of virus lacking an intact envelope and there was an increase...
Source: Virology - January 13, 2016 Category: Virology Authors: Changotra H, Turk SM, Artigues A, Thakur N, Gore M, Muggeridge MI, Hutt-Fletcher LM Tags: Virology Source Type: research

Abstract 2958: Discovering therapeutic epigenetic targets using whole genome siRNA screening
Conclusions: A whole genome siRNA screen in combination with the DNMT inhibitor decitabine identified many new target genes that might be epigenetic regulators and potential targets for drug development.Citation Format: Yasuyuki Okamoto, Woonbok Chung, Judith Garriga, Jaroslav Jelinek, Jean-Pierre J. Issa. Discovering therapeutic epigenetic targets using whole genome siRNA screening. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2958. doi:10.1158/1538-7445.AM2015-2958
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Okamoto, Y., Chung, W., Garriga, J., Jelinek, J., Issa, J.-P. J. Tags: Molecular and Cellular Biology Source Type: research

Human cytomegalovirus miR-UL36-5p inhibits apoptosis via downregulation of adenine nucleotide translocator 3 in cultured cells
In this study, ANT3 was further demonstrated to be a direct target of hcmv-miR-UL36-5p by luciferase reporter assays. The expression level of ANT3 protein was confirmed, by western blotting, to be directly downregulated by overexpression of hcmv-miR-UL36-5p in HEK293 cells, U373 cells and HELF cells. Moreover, HCMV-infected cells showed a decrease in the ANT3 protein level. Using ANT3-specific small interfering RNA (siRNA) and an inhibitor for hcmv-miR-UL36-5p, it was shown that inhibition of apoptosis by hcmv-miR-UL36-5p in these cells specifically occurred via inhibition of ANT3 expression. These results imply that hcmv-...
Source: Archives of Virology - July 28, 2015 Category: Virology Source Type: research

Abstract 373: An siRNA screen identifies CHD4 as a target for epigenetic therapy
In this study, we used an unbiased screen to investigate therapeutic targets which might be effective combination with DNMT inhibitors in reactivating hypermethylated genes. Methods: We screened an siRNA library targeting 188 gene predicted as epigenetic regulators using colon cancer cells that harbor a GFP locus stably integrated and silenced by a hypermethylated cytomegalovirus (CMV) promoter. GFP-positive cell percentages were measured using Guava EasyCyte Plus flow analyzer software. For genome wide gene expression analysis, affymetrix microarrays were performed. DNA methylation status was evaluated by pyrosequencing a...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Okamoto, Y., Yamazaki, J., Sato, T., Cesaroni, M., Chung, W., Garriga, J., Jelinek, J., Katz, R. A., Issa, J.-P. Tags: Molecular and Cellular Biology Source Type: research

Abstract 1405: Knockdown ARID5A suppresses proliferation of LNCaP prostate cancer cell through the inhibition of global protein synthesis
In this study, we report that the knockdown of Arid5a expression inhibits proliferation of LNCaP cells on the contrary to the expectation from the transient transfection assay. We found that proliferation of cells stimulated by the treatment with dihydro-testosterone (DHT) was suppressed when Arid5a expression was down-regulated by siRNA technology. Flow cytometer analysis showed that DHT-stimulated cell cycle was arrested at G1 phase after the knockdown of Arid5a expression. Oil Red O staining assays showed that the inhibition of Arid5a expression led to decreased DHT-induced lipid accumulation. Our data further proved th...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Sun, C., Chesnokov, V., Itakura, K. Tags: Molecular and Cellular Biology Source Type: research

Decrease of Murine Cytomegalovirus-Induced Retinitis by Intravenous Delivery of Immediate Early Protein-3-Specific siRNA Immunology and Microbiology
Conclusions. Systemic administration of IE-3–specific siRNA could alleviate MCMV retinitis by inhibiting virus replication and subsequent death of uninfected retinal cells.
Source: Investigative Ophthalmology - July 8, 2014 Category: Opthalmology Authors: Marshall, B., Mo, J., Covar, J., Atherton, S. S., Zhang, M. Tags: Immunology and Microbiology Source Type: research

Proteomic analysis of the multimeric nuclear egress complex of human cytomegalovirus.
Abstract Herpesviral capsids are assembled in the host cell nucleus before being translocated into the cytoplasm for further maturation. The crossing of the nuclear envelope represents a major event that requires formation of the nuclear egress complex (NEC). Previous studies demonstrated that human cytomegalovirus (HCMV) proteins pUL50 and pUL53, as well as their homologs in all members of Herpesviridae, interact with each other at the nuclear envelope and form the heterodimeric core of the NEC. In order to characterize further the viral and cellular protein content of the multimeric NEC, the native complex was i...
Source: Molecular and Cellular Proteomics : MCP - June 26, 2014 Category: Molecular Biology Authors: Milbradt J, Kraut A, Hutterer C, Sonntag E, Schmeiser C, Ferro M, Wagner S, Lenac T, Claus C, Pinkert S, Hamilton ST, Rawlinson WD, Sticht H, Coute Y, Marschall M Tags: Mol Cell Proteomics Source Type: research

Over-expression of human cytomegalovirus miR-US25-2-3p downregulates eIF4A1 and inhibits HCMV replication
Highlights: Abstract: It has been reported that human cytomegalovirus (HCMV) miR-US25-2 reduces DNA viral replication including HCMV. However, the mechanism remains unknown. In our study, eukaryotic translation initiation factor 4A1 (eIF4A1) was identified to be a direct target of miR-US25-2-3p. Small interfering RNA (siRNA) and miR-US25-2-3p mediated eIF4A1 knockdown experiments revealed that high level of miR-US25-2-3p in MRC-5 cells decreased HCMV and host genomic DNA synthesis, and inhibited cap-dependent translation and host cell proliferation. However, eIF4A1 up-regulation induced by miR-US25-2-3p inhibitor increased...
Source: FEBS Letters - June 6, 2013 Category: Biochemistry Authors: Manlong Qi, Ying Qi, Yanping Ma, Rong He, Yaohua Ji, Zhengrong Sun, Qiang Ruan Tags: Research Letters Source Type: research

Herpesviruses and the microbiome
The focus of this article will be to examine the role of common herpesviruses as a component of the microbiome of atopic patients and to review clinical observations suggesting that atopic patients might be predisposed to more severe and atypical herpes-related illness because their immune response is biased toward a TH2 cytokine profile. Human populations are infected with 8 herpesviruses, including herpes simplex virus HSV1 and HSV2 (also termed HHV1 and HHV2), varicella zoster virus (VZV or HHV3), EBV (HHV4), cytomegalovirus (HHV5), HHV6, HHV7, and Kaposi sarcoma–associated herpesvirus (termed KSV or HHV8). Herpesviru...
Source: Journal of Allergy and Clinical Immunology - April 22, 2013 Category: Allergy & Immunology Authors: David H. Dreyfus Tags: Rostrum Source Type: research