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Source: PLoS One
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Total 74 results found since Jan 2013.
LINC01117 inhibits invasion and migration of lung adenocarcinoma through influencing EMT process
ConclusionsLINC01117 was highly expressed in LUAD, and knockdown of LINC01117 significantly inhibited the migration and invasion of LUAD cells, while overexpression of LINC01117 significantly promoted the migration and invasion of LUAD cells, and affected the EMT process, and was able to alter the distribution of YAP1 in the nucleus and cytoplasm. This suggests that LINC01117 may regulate the activity of the Hippo pathway by altering the nuclear and cytoplasmic distribution of YAP1, which in turn induces the EMT process in lung adenocarcinoma cells and thus exerts a pro-cancer effect. It suggests that LINC01117 may play a ...
Source: PLoS One - June 29, 2023 Category: Biomedical Science Authors: Linjun Liu Source Type: research
MIC26 and MIC27 are bona fide subunits of the MICOS complex in mitochondria and do not exist as glycosylated apolipoproteins
by Melissa Lubeck, Nick H. Derkum, Ritam Naha, Rebecca Strohm, Marc D. Driessen, Bengt-Frederik Belgardt, Michael Roden, Kai St ühler, Ruchika Anand, Andreas S. Reichert, Arun Kumar Kondadi
Impairments of mitochondrial functions are linked to human ageing and pathologies such as cancer, cardiomyopathy, neurodegeneration and diabetes. Specifically, aberrations in ultrastructure of mitochondrial inner membrane (IM) and factors regulating them are linked to diabetes. The development of diabetes is connected to the ‘Mitochondrial Contact Site and Cristae Organising System’ (MICOS) complex which is a large membrane protei...
Source: PLoS One - June 6, 2023 Category: Biomedical Science Authors: Melissa Lubeck Source Type: research
Induction of pro-inflammatory genes by fibronectin DAMPs in three fibroblast cell lines: Role of TAK1 and MAP kinases
by Pranav Maddali, Anthony Ambesi, Paula J. McKeown-Longo
Changes in the organization and structure of the fibronectin matrix are believed to contribute to dysregulated wound healing and subsequent tissue inflammation and tissue fibrosis. These changes include an increase in the EDA isoform of fibronectin as well as the mechanical unfolding of fibronecti n type III domains. In previous studies using embryonic foreskin fibroblasts, we have shown that fibronectin’s EDA domain (FnEDA) and the partially unfolded first Type III domain (FnIII-1c) function as Damage Associated Molecular Pattern (DAMP) molecules to stimulate th...
Source: PLoS One - May 25, 2023 Category: Biomedical Science Authors: Pranav Maddali Source Type: research
The RNA-binding protein NANOS1 controls hippocampal synaptogenesis
by Dar ío Maschi, Ana J. Fernández-Alvarez, Graciela Lidia Boccaccio
Proteins from the NANOS family are conserved translational repressors with a well-known role in gonad development in both vertebrates and invertebrates. In addition,Drosophila Nanos controls neuron maturation and function, and rodent Nanos1 affects cortical neuron differentiation. Here we show that ratNanos1 is expressed in hippocampal neurons and that the siRNA-mediated knockdown ofNanos1 impairs synaptogenesis. We found that both dendritic spine size and number were affected byNanos1 KD. Dendritic spines were smaller and more numerous. Moreover, wher...
Source: PLoS One - April 14, 2023 Category: Biomedical Science Authors: Dar ío Maschi Source Type: research
A seeding-based neuronal model of tau aggregation for use in drug discovery
by Ines S. Amorim, Sylvie Challal, Laetitia Cistarelli, Thierry Dorval, Laurene Abjean, Manuelle Touzard, Nicolas Arbez, Arnaud Fran çois, Fany Panayi, Ross Jeggo, Erika Cecon, Atsuro Oishi, Julie Dam, Ralf Jockers, Patricia Machado
Intracellular accumulation of tau protein is a hallmark of Alzheimer’s Disease and Progressive Supranuclear Palsy, as well as other neurodegenerative disorders collectively known as tauopathies. Despite our increasing understanding of the mechanisms leading to the initiation and progression of t au pathology, the field still lacks appropriate disease models to facilitate drug discovery. Her...
Source: PLoS One - April 4, 2023 Category: Biomedical Science Authors: Ines S. Amorim Source Type: research
Axl contributes to efficient migration and invasion of melanoma cells
by Hanshuang Shao, Diana Teramae, Alan Wells
Axl, a member of the TAM receptor family has been broadly suggested to play a key role in tumor metastasis. However, the function of Axl in the invasion and metastasis of melanoma, the most lethal skin cancer, remains largely unknown. In the present study, we found that melanoma cell lines present variable protein levels of Axl and Tyro3; interestingly, MerTK is not noted at detectable levels in any of tested MGP (metastatic growth phase) cell lines. Treatment with recombinant human Gas6 significantly activates Akt in the Axl-expressing WM852 and IgR3 lines but just slightly in...
Source: PLoS One - March 29, 2023 Category: Biomedical Science Authors: Hanshuang Shao Source Type: research
Aging decreases docosahexaenoic acid transport across the blood-brain barrier in C57BL/6J mice
by Takuro Iwao, Fuyuko Takata, Junichi Matsumoto, Hisataka Aridome, Miho Yasunaga, Miki Yokoya, Yasufumi Kataoka, Shinya Dohgu
Nutrients are actively taken up by the brain via various transporters at the blood–brain barrier (BBB). A lack of specific nutrients in the aged brain, including decreased levels of docosahexaenoic acid (DHA), is associated with memory and cognitive dysfunction. To compensate for decreased brain DHA, orally supplied DHA must be transported from the circulating blood to the brain across the BBB through transport carriers, including major facilitator superfamily domain-containing protein 2a (MFSD2...
Source: PLoS One - February 16, 2023 Category: Biomedical Science Authors: Takuro Iwao Source Type: research
Differential effects of < i > WRAP53 < /i > transcript variants on non-small cell lung cancer cell behaviors
ConclusionsWRAP53 transcript variants exerted different functions in non-small cell lung cancer cells and regulated non-small cell lung cancer cell behaviors depending on the p53 expression.
Source: PLoS One - January 27, 2023 Category: Biomedical Science Authors: Yan Zhu Source Type: research
TRPV4 is activated by mechanical stimulation to induce prostaglandins release in trabecular meshwork, lowering intraocular pressure
by Takatoshi Uchida, Shota Shimizu, Reiko Yamagishi, Suzumi M. Tokuoka, Yoshihiro Kita, Rei Sakata, Megumi Honjo, Makoto Aihara
Trabecular meshwork constitutes the conventional outflow pathway and controls intraocular pressure by regulating aqueous outflow. Mechanical stimulation has been studied as one of the triggers to regulate aqueous outflow in trabecular meshwork, but it is not well understood. We investigated that h ow transient receptor potential cation channel subfamily V member 4 (TRPV4) functions in human trabecular meshwork cells (HTMC) and affects intraocular pressure (IOP). HTMC were treated with TRPV4 siRNA...
Source: PLoS One - October 21, 2021 Category: Biomedical Science Authors: Takatoshi Uchida Source Type: research
Plasticity of seven-transmembrane-helix receptor heteromers in human vascular smooth muscle cells
by Lauren J. Albee, Xianlong Gao, Matthias Majetschak
Recently, we reported that the chemokine (C-X-C motif) receptor 4 (CXCR4) and atypical chemokine receptor 3 (ACKR3) heteromerize with α1A/B/D-adrenoceptors (ARs) and arginine vasopressin receptor 1A (AVPR1A) in recombinant systems and in rodent and human vascular smooth muscle cells (hVSMCs). In these studies, we observed that heteromerization between two receptor partners may depend on the presence and the expression levels of other partnering receptors. To test this hypothesis and to gain initial insight into the formation of these receptor heteromers in native cell...
Source: PLoS One - June 24, 2021 Category: Biomedical Science Authors: Lauren J. Albee Source Type: research
Interleukin-1 β-induced matrix metalloproteinase-3 via ERK1/2 pathway to promote mesenchymal stem cell migration
In conclusion, we have found that IL-1β induces the expression of MMP-3 through ERK1/2, JNK, p38 MAPK and Akt signaling pathways to enhance the migration of hUCMSCs. These results provide further understanding of the mechanisms in IL-1β-induced hUCMSCs migration to injury sites.
Source: PLoS One - May 21, 2021 Category: Biomedical Science Authors: Chun-Hao Chang Source Type: research
Delivery of gene targeting siRNAs to breast cancer cells using a multifunctional peptide complex that promotes both targeted delivery and endosomal release
This study demonstrates the ability of a peptide-based siRNA-delivery system to deliver siRNA into breast cancer cells and cause both protein knockdown and su ppression of the malignant phenotype. Such peptide complexes are likely to become highly useful siRNA-delivery vehicles for the characterization, and potentially for the treatment, of human cancer.
Source: PLoS One - June 30, 2017 Category: Biomedical Science Authors: Jeffrey D. Bjorge Source Type: research
MicroRNA-433-3p promotes osteoblast differentiation through targeting DKK1 expression
In this study, mouse primary bone marrow cells and osteoblast cell lines were used. Lucifer ase reporter assay and western blotting methods were employed to validate if miRNA-433-3p epigenetically regulated DKK1 translation. Rat bone marrow derived osteoblasts were infected with lentivirus vector in which miR-433-3p was constructed. The authors constructed lentivirus mediated miRNA-433-3p stable expression and examined the alkaline phosphatase (ALP) activity and mineral deposition level in vitro. In situ hybridization method was used to observe miR-433-3p in primary osteoblasts. We built up an OVX rat model to mimic postme...
Source: PLoS One - June 19, 2017 Category: Biomedical Science Authors: Xiaolin Tang Source Type: research
Molecular cloning, characterization and expression analysis of Frizzled 6 in the small intestine of pigs ( < i > Sus scrofa < /i > )
In this study, the full-length cDNA ofSus scrofa FZD6 (Sfz6) was cloned and characterized. Nucleotide sequence analysis demonstrates that theSfz6 gene encodes the 712 amino-acid (aa) protein with seven transmembrane domain. Tissue distribution analysis showed thatSfz6 mRNA is ubiquitously expressed in various tissues, being highest in kidney, moderate in jejunum, ileum, colon, liver, and spleen. However, FZD6 protein is highly expressed in the heart and there was no significant difference in other tissues. The relative abundance and localization of FZD6 protein in jejunum along the crypt-villus axis was determined by Weste...
Source: PLoS One - June 14, 2017 Category: Biomedical Science Authors: Lijun Zou Source Type: research
Fluvastatin inhibits AGE-induced cell proliferation and migration via an ERK5-dependent Nrf2 pathway in vascular smooth muscle cells
by Ae-Rang Hwang, Jung-Hwa Han, Jae Hyang Lim, Young Jin Kang, Chang-Hoon Woo
Advanced glycation endproduct (AGE)-induced vascular smooth muscle cell (VSMC) proliferation and reactive oxygen species (ROS) production are emerging as important mechanisms of diabetic vasculopathy, but little is known about the molecular mechanism responsible for the antioxidative effects of st atins on AGEs. It has been reported that statins exert pleiotropic effects on the cardiovascular system due to decreases in AGE-induced cell proliferation, migration, and vascular inflammation. Thus, in the present study, the authors investigated the m...
Source: PLoS One - May 22, 2017 Category: Biomedical Science Authors: Ae-Rang Hwang Source Type: research
