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Total 74 results found since Jan 2013.

Molecular characterization, expression of chicken TBK1 gene and its effect on IRF3 signaling pathway
In conclusion, our results demonstrated that chTBK1 may be an important immunoregulator for IRF3 and IFN β induction in response to viral stimulation in chicken.
Source: PLoS One - May 11, 2017 Category: Biomedical Science Authors: Yan Wang Source Type: research

Downregulation of ANP32B exerts anti-apoptotic effects in hepatocellular carcinoma
In this study, we elucidated the role of ANP32B in HCC cell lines and tissues. ANP32B expression in HCC cell lines was modulated using siRNA and ANP32B expression plasmids and lentiviruses. The levels of apoptosis-related proteins were analyzed by real-time RT-PCR and Western blotting. The expressi on of ANP32B in tissues from patients with HCC was investigated using real-time RT-PCR and immunohistochemistry. ANP32B knockdown by siRNA altered the expression of apoptosis-related proteins in HCC cell lines and reduced the expression of cleaved forms of caspase 3 and caspase 9, but not that of ca spase 8, in HCC cells culture...
Source: PLoS One - May 9, 2017 Category: Biomedical Science Authors: Yoshinori Ohno Source Type: research

Expression of teneurins is associated with tumor differentiation and patient survival in ovarian cancer
by Rebecca Graumann, Gabriella A. Di Capua, Juan E. Oyarz ún, Marcos A. Vásquez, Christine Liao, Jorge A. Brañes, Iván Roa, Paola Casanello, Alejandro H. Corvalán, Gareth I. Owen, Iris Delgado, Uwe Zangemeister-Wittke, Annemarie Ziegler Teneurins are a family of highly conserved pair-rule proteins involved in morphogenesis and development of the central nervous system. Their function in adult tissues and in disease is largely unknown. Recent evidence suggests a role for dysregulated expression of Teneurins in human tumors, but sy stematic investigations are missing. Here, we investigated Teneurin-2 and Teneurin-4 exp...
Source: PLoS One - May 4, 2017 Category: Biomedical Science Authors: Rebecca Graumann Source Type: research

Tonic suppression of PCAT29 by the IL-6 signaling pathway in prostate cancer: Reversal by resveratrol
In this study we focused on the lncRNA, prostate cancer associated transc ript 29 (PCAT29), a putative tumor suppressive gene. Our data show that the expression ofPCAT29 was reduced in prostate cancer tumors compared to paired perinormal prostate tissues. We also observed substantially lower levels ofPCAT29 in DU145 and LNCaP cells compared to normal prostate (RWPE-1) cells. IL-6, a cytokine which is elevated in prostate tumors, reduced the expression ofPCAT29 in both DU145 and LNCaP cells by activating signal transducer and activator of transcription 3 (STAT3). One downstream target of STAT3 is microRNA(miR)-21, inhibitio...
Source: PLoS One - May 3, 2017 Category: Biomedical Science Authors: Raheem F. H. Al Aameri Source Type: research

CREG1 heterozygous mice are susceptible to high fat diet-induced obesity and insulin resistance
This study uncovered a novel function of CREG1 in metabolic disorders.
Source: PLoS One - May 1, 2017 Category: Biomedical Science Authors: Xiaoxiang Tian Source Type: research

p53 pathway determines the cellular response to alcohol-induced DNA damage in MCF-7 breast cancer cells
by Ming Zhao, Erin W. Howard, Zhiying Guo, Amanda B. Parris, Xiaohe Yang Alcohol consumption is associated with increased breast cancer risk; however, the underlying mechanisms that contribute to mammary tumor initiation and progression are unclear. Alcohol is known to induce oxidative stress and DNA damage; likewise, p53 is a critical modulator of the DNA repair pathw ay and ensures genomic integrity. p53 mutations are frequently detected in breast and other tumors. The impact of alcohol on p53 is recognized, yet the role of p53 in alcohol-induced mammary carcinogenesis remains poorly defined. In our study, we measured a...
Source: PLoS One - April 3, 2017 Category: Biomedical Science Authors: Ming Zhao Source Type: research

RhoA promotes epidermal stem cell proliferation via PKN1-cyclin D1 signaling
ConclusionThis study shows that the effect of RhoA on ESC proliferation is mediated by activation of the PKN1-cyclin D1 pathway in vitro, suggesting that RhoA may serve as a new therapeutic target for wound healing.
Source: PLoS One - February 20, 2017 Category: Biomedical Science Authors: Fan Wang Source Type: research

Knockdown of Mediator Complex Subunit 19 Suppresses the Growth and Invasion of Prostate Cancer Cells
In this study, by using immunohistochemical staining, we found higher expr ession level of Med19 in PCa tissues than in adjacent benign prostate tissues. We then knocked down the Med19 expression in PCa cell lines LNCaP and PC3 by using lentivirus siRNA. Cell proliferation, anchor-independent growth, migration, and invasion were suppressed in Med19 knockdown PCa cells. In nude mice xenograft model, we found that Med19 knockdown PCa cells formed smaller tumors with lower proliferation index than did control cells. In the mechanism study, we found that Med19 could regulate genes involved in cell proliferation, cell cycle, an...
Source: PLoS One - January 25, 2017 Category: Biomedical Science Authors: Shengqiang Yu Source Type: research

α-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression
by Min A. Jang, Seung Jin Lee, Seung Eun Baek, So Youn Park, Young Whan Choi, Chi Dae Kim α-Iso-cubebene (ICB) is a dibenzocyclooctadiene lignin contained in Schisandra chinensis (SC), a well-known medicinal herb that ameliorates cardiovascular symptoms. Thus, we examined the effect of ICB on vascular smooth muscle cell (VSMC) proliferation, a key feature of diverse vascular diseases. When VSMCs primary cultured from rat thoracic aorta were stimulated with PDGF (1–10 ng/ml), cell proliferation and osteopontin (OPN) expression were concomitantly up-regulated, but these effects were attenuated when cells were treated wit...
Source: PLoS One - January 22, 2017 Category: Biomedical Science Authors: Min A. Jang Source Type: research

Endogenous Semaphorin-7A Impedes Human Lung Fibroblast Differentiation
In conclusion, our study indicates that endogenous semaphorin-7A in HLF plays a role in maintaining fibroblast homeostasis by preventing up-regulation of pro-fibrotic genes. Therefore, endogenous and exogenous semaphorin-7A may have opposite effects on the fibroblast phenotype.
Source: PLoS One - January 16, 2017 Category: Biomedical Science Authors: Stephane Esnault Source Type: research

Cutaneous Deficiency of Filaggrin and STAT3 Exacerbates Vaccinia Disease In Vivo 
ConclusionsOur findings may point to a novel role for filaggrin in early antiviral responses in skin. In wounded skin with underlying barrier defects, chronically elevated activin A levels may contribute to skin remodeling and cutaneous pathogen persistence. Inhibition of ALK5/TGF βR1 signaling may provide a novel co-therapeutic approach, together with VIG, to limit cutaneous spread of vaccinia.
Source: PLoS One - January 11, 2017 Category: Biomedical Science Authors: Yong He Source Type: research

Chemokine Ligand 5 (CCL5) Derived from Endothelial Colony-Forming Cells (ECFCs) Mediates Recruitment of Smooth Muscle Progenitor Cells (SPCs) toward Critical Vascular Locations in Moyamoya Disease
In this study, co-culture of ECFCs and SPCs from MMD patients and healthy normal subjects revealed that MMD ECFCs, not SPCs, are responsible for the defective functions of both ECFCs and SPCs. Enhanced migration of SPCs toward MMD ECFCs supported the role for some chemokines secreted by MMD ECFCs. Expression arrays of MMD and normal ECFCs suggested t hat several candidate cytokines differentially produced by MMD ECFCs. We selected chemokine (C-X-C motif) ligand 6 (CXCR6), interleukin-8 (IL8), chemokine (C-C motif) ligand 2 (CCL2), and CCL5 for study, based on the relatively higher expression of these ligands in MMD ECFCs a...
Source: PLoS One - January 9, 2017 Category: Biomedical Science Authors: Ji Hoon Phi Source Type: research

Src Inhibition Can Synergize with Gemcitabine and Reverse Resistance in Triple Negative Breast Cancer Cells via the AKT/c-Jun Pathway
ConclusionIn highly gemcitabine-resistant 231 cells, src inhibition can synergize with gemcitabine, reverse drug resistance, inhibit tumor growth/metastasis/stemness of cancer stem cells, possibly via the AKT/c-Jun pathway.
Source: PLoS One - December 29, 2016 Category: Biomedical Science Authors: Zhen-Hua Wu Source Type: research

A Convenient In Vivo Model Using Small Interfering RNA Silencing to Rapidly Assess Skeletal Gene Function
by Wen Zhang, Can Liu, Bao Hai, Guohong Du, Hong Wang, Huijie Leng, Yingsheng Xu, Chunli Song It is difficult to study bone in vitro because it contains various cell types that engage in cross-talk. Bone biologically links various organs, and it has thus become increasingly evident that skeletal physiology must be studied in an integrative manner in an intact animal. We developed a model u sing local intraosseous small interfering RNA (siRNA) injection to rapidly assess the effects of a target gene on the local skeletal environment. In this model, 160-g male Sprague-Dawley rats were treated for 1–2 weeks. The left tibia...
Source: PLoS One - November 27, 2016 Category: Biomedical Science Authors: Wen Zhang Source Type: research

Atorvastatin Alleviates Experimental Diabetic Cardiomyopathy by Regulating the GSK-3 β-PP2Ac-NF-κB Signaling Axis
by Xiao-min Ren, Guang-feng Zuo, Wen Wu, Jie Luo, Peng Ye, Shao-liang Chen, Zuo-ying Hu Recent studies reported that atorvastatin (ATOR) alleviated progression of experimental diabetic cardiomyopathy (DCM), possibly by protecting against apoptosis. However, the underlying mechanisms of this protective effect remain unclear. Therefore, our study investigated the role of the glycogen s ynthase kinase (GSK)-3β-protein phosphatase 2A(PP2A)-NF-κB signaling pathway in the anti-apoptotic and cardioprotective effects of ATOR on cardiomyocytes cultured in high glucose (HG) and in DCM. Our results showed that, in HG-cultured card...
Source: PLoS One - November 15, 2016 Category: Biomedical Science Authors: Xiao-min Ren Source Type: research

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