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Source: Biology of Reproduction

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Total 100 results found since Jan 2013.

The IL6/sIL-6R α trans-signaling increases PGE2 production in human granulosa cells
In conclusion, IL6 trans-signaling upregulates the expression of PTGS2 and increases the production of PGE2 via the JAK2/STAT3/SOCS3 signaling pathway in hGL cells. Our findings provide insights into the molecular mechanisms by which IL6 trans-signaling may potentially modulate the event of ovulation in human ovaries.PMID:34198336 | DOI:10.1093/biolre/ioab128
Source: Biology of Reproduction - July 1, 2021 Category: Reproduction Medicine Authors: Sai-Jiao Li Hsun-Ming Chang Jiamin Xie Jeremy H Wang Jing Yang Peter C K Leung Source Type: research

Functional roles of the chromatin remodeler SMARCA5 in mouse and bovine preimplantation embryos
Biol Reprod. 2021 Apr 23:ioab081. doi: 10.1093/biolre/ioab081. Online ahead of print.ABSTRACTUpon fertilization, extensive chromatin reprogramming occurs during preimplantation development. Growing evidence reveals species-dependent regulations of this process in mammals. ATP-dependent chromatin remodeling factor SMARCA5 (also known as SNF2H) is required for peri-implantation development in mice. However, the specific functional role of SMARCA5 in preimplantation development and if it is conserved among species remain unclear. Herein, comparative analysis of public RNA-seq datasets reveals that SMARCA5 is universally expre...
Source: Biology of Reproduction - April 26, 2021 Category: Reproduction Medicine Authors: Yan Shi Panpan Zhao Yanna Dang Shuang Li Lei Luo Bingjie Hu Shaohua Wang Huanan Wang Kun Zhang Source Type: research

EZH2 is essential for spindle assembly regulation and chromosomal integrity during porcine oocyte meiotic maturation †.
EZH2 is essential for spindle assembly regulation and chromosomal integrity during porcine oocyte meiotic maturation†. Biol Reprod. 2020 Nov 27;: Authors: Cai Q, Wen K, Ma M, Chen W, Mo D, He Z, Chen Y, Cong P Abstract Enhancer of zeste homolog 2 (EZH2) has been extensively investigated to participate in diverse biological processes, including carcinogenesis, the cell cycle, X-chromosome inactivation and early embryonic development. However, the functions of this protein during mammalian oocyte meiotic maturation remain largely unexplored. Here, combined with RNA-Seq, we provided evidence that EZH2 ...
Source: Biology of Reproduction - November 27, 2020 Category: Reproduction Medicine Authors: Cai Q, Wen K, Ma M, Chen W, Mo D, He Z, Chen Y, Cong P Tags: Biol Reprod Source Type: research

The SARS-CoV-2 receptor, Angiotensin converting enzyme 2 (ACE2) is required for human endometrial stromal cell decidualization.
Abstract The coronavirus disease 2019 (COVID-19) first appeared in December 2019 and rapidly spread throughout the world. The SARS-CoV-2 virus enters the host cells by binding to the angiotensin-converting enzyme (ACE2). Although much of the focus is on respiratory symptoms, recent reports suggest that SARS-CoV-2 can cause pregnancy complications such as pre-term birth and miscarriages; and women with COVID-19 had maternal vascular malperfusion and decidual arteriopathy in their placentas. Here, we report that ACE2 protein is expressed in both endometrial epithelial and stromal cells in the proliferative phase of ...
Source: Biology of Reproduction - November 18, 2020 Category: Reproduction Medicine Authors: Chadchan SB, Popli P, Maurya VK, Kommagani R Tags: Biol Reprod Source Type: research

Sirtuin-1 Inhibits Endothelin-2 Expression in Human Granulosa-Lutein Cells via HIF1A and Epigenetic Modifications †.
Sirtuin-1 Inhibits Endothelin-2 Expression in Human Granulosa-Lutein Cells via HIF1A and Epigenetic Modifications†. Biol Reprod. 2020 Oct 28;: Authors: Szymanska M, Manthe S, Shrestha K, Girsh E, Harlev A, Kisliouk T, Meidan R Abstract Endothelin-2 (EDN2) expression in granulosa cells was previously shown to be highly dependent on the hypoxic mediator, HIF1A. Here we investigated whether sirtuin-1 (SIRT1), by deacetylating HIF1A and class III histones, modulates EDN2 in human granulosa-lutein cells (hGLCs). We found that HIF1A was markedly suppressed in the presence of resveratrol or a specific SIRT...
Source: Biology of Reproduction - October 28, 2020 Category: Reproduction Medicine Authors: Szymanska M, Manthe S, Shrestha K, Girsh E, Harlev A, Kisliouk T, Meidan R Tags: Biol Reprod Source Type: research

ORP9 Knockdown Delays the Maturation of Junction Related Endocytic Structures in the Testis and Leads to Impaired Sperm Release †.
ORP9 Knockdown Delays the Maturation of Junction Related Endocytic Structures in the Testis and Leads to Impaired Sperm Release†. Biol Reprod. 2020 Sep 09;: Authors: Adams A, Vogl W Abstract The release of late spermatids from the seminiferous epithelium requires the internalization of intercellular junctions by Sertoli cell specific structures called 'tubulobulbar complexes' (TBCs). These large, endocytic devices likely evolved from classic clathrin-mediated endocytosis machinery (CME), but have several important morphological differences to CME vesicles. Most notable among these differences is tha...
Source: Biology of Reproduction - September 8, 2020 Category: Reproduction Medicine Authors: Adams A, Vogl W Tags: Biol Reprod Source Type: research

Spatiotemporal expression of aquaporin 9 is critical for the antral growth of mouse ovarian follicles ††.
Spatiotemporal expression of aquaporin 9 is critical for the antral growth of mouse ovarian follicles††. Biol Reprod. 2020 Jun 24;: Authors: Lee SE, Kang HK, Ryou C, Cheon YP Abstract Although a few aquaporins (AQPs) expressed in granulosa cells have been postulated to mediate fluid passage into the antrum, the specific expression of AQPs in different follicle cell types and stages and their roles have not been evaluated extensively. The spatiotemporal expression of aquaporin (Aqp) 7, 8, and 9 and the functional roles of Aqp9 in antral growth and ovulation were examined using a superovulation mode...
Source: Biology of Reproduction - June 23, 2020 Category: Reproduction Medicine Authors: Lee SE, Kang HK, Ryou C, Cheon YP Tags: Biol Reprod Source Type: research

Interleukin-13 receptor subunit alpha-2 is a target of progesterone receptor and steroid receptor coactivator-1 in the mouse uterus †.
In this study, we employed PGR knockout mice (PRKO) and steroid receptor coactivator-1 knockout mice (SRC-1-/-) to profile the expression of Il13ra2 in the murine endometrium and determine the role of these transcriptional regulators in the hormone-responsiveness of Il13ra2 expression. Furthermore, we utilized a well-established decidualization-inducing steroidogenic cocktail and a siRNA-based knockdown of IL13RA2 to determine the importance of IL13RA2 in the decidualization of primary human endometrial stromal cells. Our findings demonstrate that Il13ra2 is expressed in the sub-epithelial stroma of the murine endometrium ...
Source: Biology of Reproduction - June 18, 2020 Category: Reproduction Medicine Authors: Marquardt RM, Lee K, Kim TH, Lee B, DeMayo FJ, Jeong JW Tags: Biol Reprod Source Type: research

Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A.
Abstract Vaspin, visceral adipose tissue-derived serine protease inhibitor, is involved in the development of obesity, insulin resistance, inflammation and energy metabolism. Our previous study showed vaspin expression and its regulation in the ovary; however, the role of this adipokine in ovarian cells has never been studied. Here, we studied in vitro the effect of vaspin on various kinase signaling pathways: mitogen-activated kinase (MAP3/1), serine/threonine kinase (AKT), signal transducer and activator of transcription 3 (STAT3) protein kinase AMP (PRKAA1), protein kinase A (PKA) and on expression of nuclear f...
Source: Biology of Reproduction - March 8, 2020 Category: Reproduction Medicine Authors: Kurowska P, Mlyczyńska E, Dawid M, Dupont J, Rak A Tags: Biol Reprod Source Type: research

Yes-associated protein (YAP1) is required for proliferation and function of bovine granulosa cells in vitro$.
Abstract Yes-associated protein (YAP1) is a major component of the Hippo signaling pathway. Although the exact extracellular signals that control the Hippo pathway are currently unknown, increasing evidence supports a critical role for the Hippo pathway in embryonic development, regulation of organ size, and carcinogenesis. Granulosa cells within the ovarian follicle proliferate and produce steroids and growth factors, which facilitate growth of follicle and maturation of the oocyte. We hypothesize that YAP1 plays a role in proliferation and estrogen secretion of granulosa cells. In the current study, we examined ...
Source: Biology of Reproduction - July 26, 2019 Category: Reproduction Medicine Authors: Plewes MR, Hou X, Zhang P, Liang A, Hua G, Wood JR, Cupp AS, Lv X, Wang C, Davis JS Tags: Biol Reprod Source Type: research

Synergistic inhibition of csal1 and csal3 in granulosa cell proliferation and steroidogenesis of hen ovarian prehierarchical development.
This study aimed to explore the potential role and mechanism of csal1 and csal3 in granulosa cells (GCs) proliferation, differentiation, and follicle selection within the PFs of hen ovary. Our data demonstrated that the csal1 and csal3 transcriptions were highly expressed in GCs of the various-sized PFs and their proteins were mainly localized in the cytoplasm of granulosa cells and oocytes of the examined PFs as well as in the ovarian stroma and epithelium. It initially revealed that both csal1 and csal3 may be involved in chicken PF development via a translocation mechanism. Furthermore, our results showed an abundance o...
Source: Biology of Reproduction - July 26, 2019 Category: Reproduction Medicine Authors: Zhu H, Qin N, Xu X, Sun X, Chen X, Zhao J, Xu R, Mishra B Tags: Biol Reprod Source Type: research

Endogenous retrovirus-encoded Syncytin-2 contributes to exosome-mediated immunosuppression of T cells.
Abstract Modulation of the activation status of immune cell populations during pregnancy depends on placental villous cytotrophoblast (VCT) cells and the syncytiotrophoblast (STB) layer. Failure in the establishment of this immunoregulatory function leads to pregnancy complications. Our laboratory has been studying Syncytin-2 (Syn-2), an endogenous retroviral protein expressed in placenta and on the surface of placental exosomes. This protein plays an important role in STB formation through its fusogenic properties, but also possesses an active immunosuppressive domain (ISD). Considering that Syn-2 expression is i...
Source: Biology of Reproduction - July 17, 2019 Category: Reproduction Medicine Authors: Lokossou AG, Toudic C, Nguyen PT, Elisseeff X, Vargas A, Rassart É, Lafond J, Leduc L, Bourgault S, Gilbert C, Scorza T, Tolosa J, Barbeau B Tags: Biol Reprod Source Type: research

SMAD-dependent signaling mediates BMP6-induced stimulation of connective tissue growth factor in luteinized human granulosa cells.
In this study, we demonstrated that BMP6 treatment significantly increased the expression of CTGF in both primary and immortalized human granulosa cells. Using both pharmacological inhibitors and siRNA-mediated knockdown approaches, we showed that ALK2 and ALK3 type I receptors are required for BMP6-induced cellular activities. Furthermore, this effect is most likely mediated by a SMAD-dependent pathway. Our studies provide novel insight into the molecular mechanisms by which an intraovarian growth factor affects the production of another factor via a paracrine effect in human granulosa cells. PMID: 31210269 [PubMed -...
Source: Biology of Reproduction - June 17, 2019 Category: Reproduction Medicine Authors: Liu S, Chang HM, Yi Y, Yao YQ, Leung PCK Tags: Biol Reprod Source Type: research