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Source: Cell Biology International

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Total 159 results found since Jan 2013.

siRNA-based nanotherapeutics as emerging modalities for immune-mediated diseases: A preliminary review
This study will pave the way for new avenues of research into the diagnosis and treatment of IMDs.PMID:35830711 | DOI:10.1002/cbin.11841
Source: Cell Biology International - July 13, 2022 Category: Cytology Authors: Saman Sargazi Rabia Arshad Reza Ghamari Abbas Rahdar Ali Bakhshi Sonia Fathi Karkan Narges Ajalli Muhammad Bilal Ana M D íez-Pascual Source Type: research

Gene expression profile analysis of SUDHL6 cells with siRNA-mediated BCL11 A downregulation.
Abstract Our previous study has shown that downregulation of B-cell chronic lymphocytic leukemia (CLL)/lymphoma11A (BCL11A) gene by small interfering RNA (siRNA) results in the growth inhibition and apoptosis of B cell lymphoma cell line SUDHL6. To gain further insight into the molecular mechanisms of this process and identify the differentially expressed genes in SUDHL6 cells after BCL11A downregulation, the global gene expression profile was identified and analyzed using the Affymetrix HG-U133 Plus 2.0 array.Twenty-one differentially expressed genes were validated and analyzed from the BCL11 A siRNA-treated SUDH...
Source: Cell Biology International - July 7, 2014 Category: Cytology Authors: Wu H, Li Y Tags: Cell Biol Int Source Type: research

A novel therapeutic drug for colon cancer: EpCAM scFv-truncated protamine (tp)-siRNA.
Abstract Colon cancer is a type of malignant tumor that causes considerable mortality worldwide. Epithelial cellular adhesion molecule (EpCAM), a tumor-associated antigen of colon tumors, is a target for colon cancer therapy. EpCAM-specific monoclonal antibodies (mAbs) have been applied in human colon cancer since the 1990s; however, the therapeutic effects are limited. EpCAM activates nuclear signaling pathways by releasing its intracellular domain (EpICD). The released EpICD stimulates the Wnt/β-catenin signaling pathway, which is also strongly associated with tumorigenesis. EpCAM is also a target gene of the W...
Source: Cell Biology International - April 10, 2013 Category: Cytology Authors: Hao H, Zhen Y, Wang Z, Chen F, Xie X Tags: Cell Biol Int Source Type: research

SiRNA-targeted carboxypeptidase D inhibits hepatocellular carcinoma growth.
Abstract Carboxypeptidase D (CPD), a membrane-bound metallocarboxypeptidase that functions as a docking receptor for duck hepatitis B virus, is frequently overexpressed in human cancers. We have explored its expression pattern, clinical significance, and biological function of CPD in hepatocellular carcinoma (HCC). CPD expression was markedly elevated in HCCs relative to adjacent non-tumor liver tissues, as determined by quantitative real-time polymerase chain reaction and Western blot analysis. Immunohistochemistry showed that 164 of 400 (41%) HCCs had high expression of CPD. CPD overexpression was significantly ...
Source: Cell Biology International - April 16, 2013 Category: Cytology Authors: Jin T, Fu J, Feng XJ, Wang SM, Huang X, Zhu MH, Zhang SH Tags: Cell Biol Int Source Type: research

NF-κBP65 promotes invasion and metastasis of oesophageal squamous cell cancer by regulating matrix metalloproteinase-9 and epithelial-to-mesenchymal transition.
Abstract NF-κB has been recognized as one of the factors responsible for the development of cancer; however, the mechanism by which high expression of NF-κB contributes to the progression of human oesophageal squamous cell cancer (ESCC) is not fully understood. In our investigations, NF-κBP65 was overexpressed in human ESCC tissues, especially in ESCC tissues with deep invasion and lymph node metastasis. Suppression of NF-κBP65 by siRNA decreased the invasion and proliferation ability of EC9706 cells in vitro. Furthermore, siRNA-mediated NF-κBP65 knock-down could lead to the downregulation of MMP-9, a metasta...
Source: Cell Biology International - March 15, 2013 Category: Cytology Authors: Wang F, He W, Wang PF, Fan Q Tags: Cell Biol Int Source Type: research

HBxAg suppresses cell apoptosis and promotes the secretion of placental hormones in human placental trophoblasts via activation of the EGFR/Akt pathway.
In conclusion, the pEGFR protein was robustly upregulated in HBx-infected human placenta tissues and trophoblast cells. HBx reduces cell apoptosis and promotes the secretion of placental hormones in human placental trophoblast cells via activation of the EGFR/Akt pathway. PMID: 29052908 [PubMed - as supplied by publisher]
Source: Cell Biology International - October 20, 2017 Category: Cytology Authors: Wang W, Bai G, Zhang Y, Zhang T, Li C, Yuan Y, Liu S, Wang C Tags: Cell Biol Int Source Type: research

Phosphorylation of Dab2 is involved in inhibited VEGF-VEGFR-2 signaling induced by downregulation of Syndecan-1 in glomerular endothelial cell.
In conclusion, we speculated that, phosphorylation of Dab2 is involved in preventing activation of VEGF-VEGFR-2 signaling in GEnC transfected with Syndecan-1 siRNA. This provide a new target for the therapy of GEnC injury and kidney disease. This article is protected by copyright. All rights reserved. PMID: 31868265 [PubMed - as supplied by publisher]
Source: Cell Biology International - December 22, 2019 Category: Cytology Authors: Jing Z, Jia-Jun W, Wei-Jie Y Tags: Cell Biol Int Source Type: research

Analysis of the expression and association of retinoblastoma binding protein 6 (RBBP6) with the JNK signaling pathway in prostate cancers.
This study aims to investigate the expression of retinoblastoma binding protein 6 (RBBP6) in prostate cancer (PCa) and its association with the c-Jun N-terminal kinase (JNK) pathway. Immunohistochemistry was used to detect RBBP6 and JNK1/2 expression in PCa and benign prostatic hyperplasia (BPH) tissues. RBBP6 expression in PCa cells (LNCap, PC3 and DU145) and noncancerous prostate epithelial cells (RWPE-1) was determined by qRT-PCR and Western blotting. PC3 and DU145 cells were transfected with RBBP6 siRNAs to examine the biological characteristics. Anisomycin (a JNK activator) with/without RBBP6 siRNA was used to treat P...
Source: Cell Biology International - July 13, 2020 Category: Cytology Authors: Guan WY, Zhao S, Luo YN Tags: Cell Biol Int Source Type: research

RNAi-mediated MMP-9 silencing inhibits mouse melanoma cell invasion and migration in vitro and in vivo.
Abstract MMP-9 participates in tumour growth, invasion, metastasis and vascularisation. Thus, inhibition of MMP-9 may be involved in the process of tumourigenesis. We have investigated the effect of RNAi-mediated MMP-9 silencing on inhibiting invasion and migration of mouse melanoma cell B16. A specific and optimised siRNA vector was used to target MMP-9 mRNA synthesis in B16 cells. Changes of invasion and migration capability of B16 cell were examined after transfection at different time, and a footpad tumour model was performed to measure the effect of MMP-9 siRNA on melanoma tumourigenesis in vivo. In vitro, do...
Source: Cell Biology International - March 30, 2013 Category: Cytology Authors: Tang ZY, Liu Y, Liu LX, Ding XY, Zhang H, Fang LQ Tags: Cell Biol Int Source Type: research

Expression and Function of Fatty Acid-binding Protein 4 in Epithelial Cell of Uterine Endometrium.
Abstract The aims of this study were to delineate the expression of fatty-acid binding protein (FABP) 4 in human uterine endometrium and its function in the regulation of proliferation, migration and invasion of epithelial cells. Immunohistochenistry, immunofluorence and Western blotting were used to determine the expression and cellular localization of FABP4 in endometrium and endometrial epithelial cell lines. Interference of small ribonuclear acid (siRNA) and specific FABP4 inhibitor were used to inhibit FABP4. The proliferation, migration and invasion of epithelial cells were evaluated with CCK-8 assay, wound-...
Source: Cell Biology International - January 9, 2015 Category: Cytology Authors: Zhu Q, Jin Y, Wang P, Wang H, Lu B, Wang Z, Dong M Tags: Cell Biol Int Source Type: research

WNT5A promotes migration and invasion of human osteosarcoma cells via SRC/ERK/MMP-14 pathway.
Abstract WNT5A, a representative ligand of activating several non-canonical Wnt signal pathways, plays significant roles in oncogenesis and tumor inhibition. It has been shown that the non-receptor tyrosine kinase SRC is required for WNT5A-induced invasion of osteosarcoma cells. However, the precise molecular mechanism underlying WNT5A/SRC-mediated osteosarcoma cells invasion remains poorly defined. The study was designed to explore the role of ERK1/2 in WNT5A/SRC-induced osteosarcoma cells invasion and the downstream target of the SRC/ERK1/2 signalings. We found that WNT5A (100ng/ml) remarkably stimulated migrati...
Source: Cell Biology International - January 18, 2018 Category: Cytology Authors: Wang X, Zhao X, Yi Z, Ma B, Wang H, Pu Y, Wang J, Wang S Tags: Cell Biol Int Source Type: research

Inhibition of CXCR4 regulates epithelial mesenchymal transition of NSCLC via the Hippo-YAP signaling pathway.
This study aims to investigate the effect of CXCR4 inhibition on epithelial-mesenchymal transition (EMT), invasion and migration of NSCLC cells via the Hippo-YAP pathway. QRT-PCR and Western blot were employed to detect CXCR4 expression in NSCLC cell lines. A549 and H1299 cells were treated with WZ811 (0, 10, 30, and 50µM), and A549 cells were also divided into the Control, WZ811, YAP siRNA, and WZ811 + YAP groups. Wound-healing, Transwell assay, immunofluorescent staining and a luciferase reporter gene assay were performed in this experiment. Compared with human bronchial epithelial (HBE) cells, CXCR4 expression was ...
Source: Cell Biology International - July 4, 2018 Category: Cytology Authors: Zheng CH, Chen XM, Zhang FB, Zhao C, Tu SS Tags: Cell Biol Int Source Type: research

Activation of NF- κB Signaling Pathway during HCG-Induced VEGF Expression in Luteal Cells.
Activation of NF-κB Signaling Pathway during HCG-Induced VEGF Expression in Luteal Cells. Cell Biol Int. 2018 Dec 31;: Authors: Zhang Z, Huang Y, Zhang J, Liu Z, Lin Q, Wang Z Abstract Vascular endothelial growth factor (VEGF) plays an essential role in luteal angiogenesis, the present study therefore utilized luteal cells cultured in vitro to further investigate the activation and contribution of nuclear factor (NF)-κB to VEGF expression induced by human chorionic gonadotrophin (HCG). The present results showed HCG induced VEGF expression as well as hypoxia-inducible factor (HIF)-1α mRNA and prote...
Source: Cell Biology International - December 31, 2018 Category: Cytology Authors: Zhang Z, Huang Y, Zhang J, Liu Z, Lin Q, Wang Z Tags: Cell Biol Int Source Type: research