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Source: Biochimica et Biophysica Acta

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Total 177 results found since Jan 2013.

Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing.
In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab' to siRNA/SMLs enhanced EGFR-specific ...
Source: Biochimica et Biophysica Acta - February 28, 2018 Category: Biochemistry Authors: Mokhtarieh AA, Lee J, Kim S, Lee MK Tags: Biochim Biophys Acta Source Type: research

siRNA release from pri-miRNA scaffolds is controlled by the sequence and structure of RNA.
In this study, using high-resolution northern blotting and small RNA sequencing, we investigated the precision with which RNases Drosha and Dicer process shmiRs. The fidelity of siRNA release from the commonly used pri-miRNA shuttles was found to depend on both the siRNA insert and pri-miR scaffold. Then, we searched for specific factors that may affect the precision of siRNA release and found that both the structural features of shmiR hairpins and the nucleotide sequence at Drosha and Dicer processing sites contribute to cleavage site selection and cleavage precision. An analysis of multiple shRNA intermediates generated ...
Source: Biochimica et Biophysica Acta - February 24, 2016 Category: Biochemistry Authors: Galka-Marciniak P, Olejniczak M, Starega-Roslan J, Szczesniak MW, Makalowska I, Krzyzosiak WJ Tags: Biochim Biophys Acta Source Type: research

Effects of siRNA-dependent knock-down of cardiolipin synthase and tafazzin on mitochondria and proliferation of glioma cells.
This study aimed to separate the effects of CL content and CL composition from cellular free fatty acid distribution on bioenergetics and proliferation in C6 glioma cells. To this end, cardiolipin synthase and the CL remodelling enzyme, tafazzin, were knocked-down by siRNA in C6 cells. After 72 h of cultivation, we analysed CL composition by means of LC/MS/MS, distribution of cellular fatty acids by means of gas chromatography, and determined oxygen consumption and proliferation. Knock-down of cardiolipin synthase affected the cellular CL content in the presence of linoleic acid (LA) in the culture medium. Knock-down of ta...
Source: Biochimica et Biophysica Acta - January 8, 2018 Category: Biochemistry Authors: Ohlig T, Le DV, Gardemann A, Wolke C, Gürtler S, Peter D, Schild L, Lendeckel U Tags: Biochim Biophys Acta Source Type: research

Conserved association of Argonaute 1 and 2 proteins with miRNA and siRNA pathways throughout insect evolution, from cockroaches to flies.
PMID: 29656113 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - April 12, 2018 Category: Biochemistry Authors: Rubio M, Maestro JL, Piulachs MD, Belles X Tags: Biochim Biophys Acta Source Type: research

siRNA enrichment in Argonaute 2-depleted Blattella germanica
Conclusions/General significance The siRNAs produced from a dsRNA mapped heterogeneously along the length of the dsRNA and this arrangement depends on the dsRNA sequence. AGO2 exerts its role as nuclease on the siRNA duplexes independently of its action on the corresponding mRNA. This study sheds light on an extremely useful process for reverse genetics in laboratories, in addition to the design of more effective, specific, and eco-friendly pest-control strategies.PMID:33895310 | DOI:10.1016/j.bbagrm.2021.194704
Source: Biochimica et Biophysica Acta - April 25, 2021 Category: Biochemistry Authors: Jos é Carlos Montañés Carlos Rojano Guillem Ylla Maria Dolors Piulachs Jos é Luis Maestro Source Type: research

A therapeutic trial of human melanomas with combined small interfering RNAs targeting adaptor molecules p130Cas and paxillin activated under expression of ganglioside GD3.
Abstract We previously demonstrated that focal adhesion kinase (FAK), p130Cas and paxillin are crucially involved in the enhanced malignant properties under expression of ganglioside GD3 in melanoma cells. Therefore, molecules existing in the GD3-mediated signaling pathway could be considered as suitable targets for therapeutic intervention in malignant melanoma. The aim of this study was to determine whether blockade of p130Cas and/or paxillin by RNAi suppresses melanoma growth. We found a suitable dose (40 μM siRNA, 25 μl/tumor) of the siRNA to suppress p130Cas in the xenografts generated in nu/nu mice. Based ...
Source: Biochimica et Biophysica Acta - April 7, 2016 Category: Biochemistry Authors: Makino Y, Hamamura K, Takei Y, Bhuiyan RH, Ohkawa Y, Ohmi Y, Nakashima H, Furukawa K, Furukawa K Tags: Biochim Biophys Acta Source Type: research

Ischemia-Induced Autophagy Contributes to Neurodegeneration in Cerebellar Purkinje Cells in the Developing Rat Brain and in Primary Cortical Neurons In Vitro.
Abstract Increased autophagy/mitophagy is thought to contribute to cerebellar dysfunction in Purkinje cell degeneration mice. Intriguingly, cerebellar Purkinje cells are highly vulnerable to hypoxia-ischemia (HI), related at least in part to their high metabolic activity. Whether or not excessive or supraphysiologic autophagy plays a role in Purkinje cell susceptibility to HI is unknown. Accordingly, we evaluated the role of autophagy in the cerebellum after global ischemia produced by asphyxial cardiac arrest in postnatal day (PND) 16-18 rats, using siRNA-targeted inhibition of Atg7, necessary for microtubule-ass...
Source: Biochimica et Biophysica Acta - June 10, 2015 Category: Biochemistry Authors: Au AK, Chen Y, Lina D, Smith CM, Manole MD, Baltagi SA, Chu CT, Aneja RK, Bayır H, Kochanek PM, Clark RS Tags: Biochim Biophys Acta Source Type: research

Superparamagnetic iron oxide nanoparticles target BxPC-3 cells and silence MUC4 for theranostics of pancreatic cancer
CONCLUSION: PEI-SPION-siRNA may be beneficial as a novel theranostic tool for PC.PMID:37236323 | DOI:10.1016/j.bbagen.2023.130383
Source: Biochimica et Biophysica Acta - May 26, 2023 Category: Biochemistry Authors: Yu Pu Helin Ke Changqiang Wu Shaodan Xu Yang Xiao Lina Han Guorong Lyv Shilin Li Source Type: research

Activation of STAT3/HIF-1 α/Hes-1 axis promotes Trastuzumab resistance in HER2- overexpressing Breast cancer cells via down-regulation of PTEN.
CONCLUSION: The impairment of STAT3-HIF-1α-HES-1 pathway restored trastuzumab sensitivity through up-regulation of PTEN protein. GENERAL SIGNIFICANCE: These findings highlighted the signal integrator role of HIF-1α in STAT3-mediated trastuzumab resistance induction which would be valuable in designing more efficient chemosensitization strategies. PMID: 28499822 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - May 9, 2017 Category: Biochemistry Authors: Aghazadeh S, Yazdanparast R Tags: Biochim Biophys Acta Source Type: research

Transfection of siRNAs can alter miRNA levels and trigger non-specific protein degradation in mammalian cells.
Abstract Sequence-non-specific effects of siRNAs that alter the expression of non-targeted genes have been reported, including competition of siRNAs with endogenous RISC components. However, the detailed mechanisms and subsequent effects of such competition are not well documented. Here we analyze the competition of miRNAs in mammalian cells with low concentrations of siRNAs, and found that: 1) transfection of different siRNAs in the low nanomolar range used to deplete target RNAs can reduce the levels of miRNAs in different cell types, 2) siRNA transfection results in rapid reduction of Ago2-associated miRNAs con...
Source: Biochimica et Biophysica Acta - February 8, 2013 Category: Biochemistry Authors: Liang XH, Hart CE, Crooke ST Tags: Biochim Biophys Acta Source Type: research

Circ100284, via miR-217 regulation of EZH2, is involved in the arsenite-accelerated cell cycle of human keratinocytes in carcinogenesis.
Abstract Circular RNAs (circRNAs), a class of noncoding RNAs generated from pre-mRNAs, participate in regulation of genes. The mechanism for regulation, however, is unknown. Here, to determine if, in human keratinocyte (HaCaT) cells, circular RNAs are involved in arsenite-induced acceleration of the cell cycle, a circRNA microarray was performed to analyze the variability of circRNAs in arsenite-treated HaCaT (As-HaCaT) cells and in arsenite-transformed (T-HaCaT) cells in comparison to control HaCaT cells. Among the circRNAs up-regulated in both As-HaCaT cells and T-HaCaT cells, hsa:circRNA_100284 (circ100284) had...
Source: Biochimica et Biophysica Acta - January 2, 2017 Category: Biochemistry Authors: Xue J, Liu Y, Luo F, Lu X, Xu H, Liu X, Lu L, Yang Q, Chen C, Fan W, Liu Q Tags: Biochim Biophys Acta Source Type: research

LAZ3 protects cardiac remodeling in diabetic cardiomyopathy via regulating miR-21/PPARa signaling.
In conclusion, LAZ3 protects against cardiac remodeling in DCM by decreasing miR-21, thus regulating PPARa/NRF2 signaling. PMID: 30031228 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - July 18, 2018 Category: Biochemistry Authors: Gao L, Liu Y, Guo S, Xiao L, Wu L, Wang Z, Liang C, Yao R, Zhang Y Tags: Biochim Biophys Acta Source Type: research

Short interfering RNA delivered by a hybrid nanoparticl ße targeting VEGF: Biodistribution and anti-tumor effect
CONCLUSION: The hybrid system prepared was successful in promoting the RNAi effect in vivo with very low toxicity.GENERAL SIGNIFICANCE: This study shows the valuable development of a hybrid nanoparticle carrying VEGF siRNA, as well as their tumor targeting, accumulation and reduction in mice triple-negative breast cancer.PMID:34062235 | DOI:10.1016/j.bbagen.2021.129938
Source: Biochimica et Biophysica Acta - June 1, 2021 Category: Biochemistry Authors: Gabriela Regina Rosa Souza Milene Dalmina Jelver Alexander Sierra Restrepo Le ônidas João de Mello Junior Adny Henrique Silva Ana Gualberto Jacy Gameiro Dalton Dittz Andr é Avelino Pasa Frederico Pittella T ânia Beatriz Creczynski-Pasa Source Type: research