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Source: Biochimica et Biophysica Acta
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Total 177 results found since Jan 2013.
Specific amino acids regulate Sestrin2 mRNA and protein levels in an ATF4-dependent manner in C2C12 myocytes
CONCLUSIONS: Specific Amino acids regulate Sestrin2 levels in an ATF4-dependent manner in C2C12 cells.GENERAL SIGNIFICANCE: The results of the present study indicate that amino acids regulate levels of Sestrin2, which might cause phenotypic alterations, including mTORC1 activity, in C2C12 cells.PMID:35597502 | DOI:10.1016/j.bbagen.2022.130174
Source: Biochimica et Biophysica Acta - May 21, 2022 Category: Biochemistry Authors: Ran Sawa Ayaka Ohnishi Maya Ohno Maika Nagata Ikumi Wake Yasuhiko Okimura Source Type: research
PPARγ-dependent pathway in the growth-inhibitory effects of K562 cells by carotenoids in combination with rosiglitazone.
CONCLUSIONS: PPARγ signaling pathway, via stimulating p21 and inhibiting cyclin D1, may play an important role in the anti-proliferative effects of carotenoid and rosiglitazone combination on K562 cells.
GENERAL SIGNIFICANCE: Carotenoids in combination with rosiglitazone are hopeful to provide attractive dietary or supplementation-based and pharmaceutical strategies to treat cancer diseases.
PMID: 24036327 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - September 11, 2013 Category: Biochemistry Authors: Zhao H, Gu H, Zhang H, Li JH, Zhao WE Tags: Biochim Biophys Acta Source Type: research
Ki-1/57 and CGI-55 ectopic expression impact cellular pathways involved in proliferation and stress response regulation.
Abstract
Ki-1/57 (HABP4) and CGI-55 (SERBP1) are regulatory proteins and paralogs with 40.7% amino acid sequence identity and 67.4% similarity. Functionally, they have been implicated in the regulation of gene expression on both the transcriptional and mRNA metabolism levels. A link with tumorigenesis is suggested, since both paralogs show altered expression levels in tumor cells and the Ki-1/57 gene is found in a region of chromosome 9q that represents a haplotype for familiar colon cancer. However, the target genes regulated by Ki-1/57 and CGI-55 are unknown. Here, we analyzed the alterations of the global trans...
Source: Biochimica et Biophysica Acta - September 6, 2014 Category: Biochemistry Authors: Costa FC, Saito A, Gonçalves KA, Vidigal PM, Meirelles GV, Bressan GC, Kobarg J Tags: Biochim Biophys Acta Source Type: research
Transforming growth factor β2 (TGF-β2)-induced connective tissue growth factor (CTGF) expression requires sphingosine 1-phosphate receptor 5 (S1P5) in human mesangial cells.
Abstract
Transforming growth factor β2 (TGF-β2) is well known to stimulate the expression of pro-fibrotic connective tissue growth factor (CTGF) in several cell types including human mesangial cells. The present study demonstrates that TGF-β2 enhances sphingosine 1-phosphate receptor 5 (S1P5) mRNA and protein expression in a time and concentration dependent manner. Pharmacological and siRNA approaches reveal that this upregulation is mediated via activation of classical TGF-β downstream effectors, Smad and mitogen-activated protein kinases. Most notably, inhibition of Gi with pertussis toxin and downregulation...
Source: Biochimica et Biophysica Acta - January 17, 2015 Category: Biochemistry Authors: Wünsche C, Koch A, Goldschmeding R, Schwalm S, Meyer Zu Heringdorf D, Huwiler A, Pfeilschifter J Tags: Biochim Biophys Acta Source Type: research
P21 Activated Kinase-1 Mediates Transforming Growth Factor β1-Induced Prostate Cancer Cell Epithelial to Mesenchymal Transition.
Abstract
Transforming growth factor beta (TGFβ) is believed to play a dual role in prostate cancer. Molecular mechanism by which TGFβ1 suppresses early prostate tumor growth and induces epithelial-to-mesenchymal transition (EMT) in advanced stages is not known. We determined if P21-activated kinase1 (Pak1), which mediates cytoskeletal remodeling is necessary for the TGFβ1 induced prostate cancer EMT. Effects of TGFβ1 on control prostate cancer PC3 and DU145 cells and those with IPA 3 and siRNA mediated Pak1 inhibition were tested for prostate tumor xenograft in vivo and EMT in vitro. TGFβ1 inhibited PC3 tumor...
Source: Biochimica et Biophysica Acta - March 5, 2015 Category: Biochemistry Authors: Al-Azayzih A, Gao F, Somanath PR Tags: Biochim Biophys Acta Source Type: research
Transforming growth factor-β3 regulates cell junction restructuring via MAPK-mediated mRNA destabilization and Smad-dependent protein degradation of Junctional adhesion molecule B (JAM-B).
This study aims to investigate the effects of TGF-β3 on the expression of JAM-B as well as the underlying mechanisms on how TGF-β3 regulates JAM-B expression to facilitate the disassembly of the BTB and apical ES. Our results revealed that TGF-β3 suppresses JAM-B at post-transcriptional and post-translational levels. Inhibitor, siRNA knockdown and co-immunoprecipitation have shown that TGF-β3 induces JAM-B protein degradation via ubiquitin-proteasome pathway. Immunofluorescence staining further confirmed that blockage of ubiquitin-proteasome pathway could abrogate TGF-β3-induced loss of JAM-B at the cell-cell interfac...
Source: Biochimica et Biophysica Acta - March 25, 2015 Category: Biochemistry Authors: Zhang X, Lui WY Tags: Biochim Biophys Acta Source Type: research
Functional characterization of the Organic Cation Transporters (OCTs) in human airway pulmonary epithelial cells.
Abstract
Organic cation transporters (OCT1-3) mediate the transport of organic cations including inhaled drugs across the cell membrane, although their role in lung epithelium hasn't been well understood yet. We address here the expression and functional activity of OCT1-3 in human airway epithelial cells A549, Calu-3 and NCl-H441. Kinetic and inhibition analyses, employing [(3)H]1-methyl-4-phenylpyridinium (MPP+) as substrate, and the compounds quinidine, prostaglandine E2 (PGE2) and corticosterone as preferential inhibitors of OCT1, OCT2, OCT3, respectively, have been performed. A549 cells present a robust MPP+ ...
Source: Biochimica et Biophysica Acta - April 13, 2015 Category: Biochemistry Authors: Ingoglia F, Visigalli R, Rotoli BM, Barilli A, Riccardi B, Puccini P, Dall'Asta V Tags: Biochim Biophys Acta Source Type: research
Hyper-dependence of breast cancer cell types on the nuclear transporter Importin β1.
Abstract
We previously reported that overexpression of members of the Importin (Imp) superfamily of nuclear transporters results in increased nuclear trafficking through conventional transport pathways in tumour cells. Here we show for the first time that the extent of overexpression of Impβ1 correlates with disease state in the MCF10 human breast tumour progression system. Excitingly, we find that targeting Impβ1 activity through siRNA is>30 times more efficient in decreasing the viability of malignant ductal carcinoma cells compared to isogenic non-transformed counterparts, and is highly potent and tumour s...
Source: Biochimica et Biophysica Acta - May 7, 2015 Category: Biochemistry Authors: Kuusisto HV, Jans DA Tags: Biochim Biophys Acta Source Type: research
Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains.
Abstract
Sphingomyelin (SM) is synthesized by SM synthase (SMS) from ceramide (Cer). SM regulates signaling pathways and maintains organ structure. SM comprises a sphingoid base and differing lengths of acyl-chains, but the importance of its various forms and regulatory synthases is not known. It has been reported that Cer synthase (CerS) has restricted substrate specificity, whereas SMS has no specificity for different lengths of acyl-chains. We hypothesized that the distribution of each SM molecular species was regulated by expression of the CerS family. Thus, we compared the distribution of SM species and CerS ...
Source: Biochimica et Biophysica Acta - September 19, 2015 Category: Biochemistry Authors: Sugimoto M, Shimizu Y, Yoshioka T, Wakabayashi M, Tanaka Y, Higashino K, Numata Y, Sakai S, Kihara A, Igarashi Y, Kuge Y Tags: Biochim Biophys Acta Source Type: research
Deletion of sphingosine kinase 1 ameliorates hepatic steatosis in diet-induced obese mice: Role of PPARγ.
Abstract
Sphingolipid metabolites have emerged playing important roles in the pathogenesis of nonalcoholic fatty liver disease, whereas the underlying mechanism remains largely unknown. In the present study, we provide both in vitro and in vivo evidence showing a pathogenic role of sphingosine kinase 1 (SphK1) in hepatocellular steatosis. We found that levels of SphK1 expression were significantly increased in steatotic hepatocytes. Enforced overexpression of SphK1 or treatment with sphingosine 1-phosphate (S1P) markedly enhanced hepatic lipid accumulation. In contrast, the siRNA-mediated knockdown of SphK1 or S1P...
Source: Biochimica et Biophysica Acta - November 23, 2015 Category: Biochemistry Authors: Chen J, Wang W, Qi Y, Kaczorowski D, McCaughan GW, Gamble JR, Don AS, Gao X, Vadas MA, Xia P Tags: Biochim Biophys Acta Source Type: research
Linoleic acid permeabilizes gastric epithelial cells by increasing Connexin43 levels in the cell membrane via a GPR40- and akt-dependent mechanism.
Abstract
Linoleic acid (LA) is known to activate G-protein coupled receptors and connexin hemichannels (Cx HCs) but possible interlinks between these two responses remain unexplored. Here, we evaluated the mechanism of action of LA on the membrane permeability mediated by Cx HCs in MKN28 cells. These cells were found to express connexins, GPR40, GPR120, and CD36 receptors. The Cx HC activity of these cells increased after 5min of treatment with LA or GW9508, an agonist of GPR40/GPR120; or exposure to extracellular divalent cation-free solution (DCFS), known to increase the open probability of Cx HCs, yields an imm...
Source: Biochimica et Biophysica Acta - February 8, 2016 Category: Biochemistry Authors: Puebla C, Cisterna BA, Salas DP, Delgado-López F, Lampe PD, Sáez JC Tags: Biochim Biophys Acta Source Type: research
A molecular insight into the electro-transfer of small molecules through electropores driven by electric fields.
Abstract
The transport of chemical compounds across the plasma membrane into the cell is relevant for several biological and medical applications. One of the most efficient techniques to enhance this uptake is reversible electroporation. Nevertheless, the detailed molecular mechanism of transport of chemical species (dyes, drugs, genetic materials …) following the application of electric pulses is not yet fully elucidated. In the past decade, molecular dynamics (MD) simulations have been conducted to model the effect of pulsed electric fields on membranes, describing several aspects of this phenomenon. Here we f...
Source: Biochimica et Biophysica Acta - March 22, 2016 Category: Biochemistry Authors: Casciola M, Tarek M Tags: Biochim Biophys Acta Source Type: research
Elatoside C protects against ox-LDL-induced HUVECs injury by FoxO1-mediated autophagy induction.
In conclusion, EsC attenuated ox-LDL-induced HUVECs injury by inducing autophagy via increasing FoxO1 expression level. EsC is thus considered as a potential drug for the treatment of atherosclerosis.
PMID: 28189723 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - February 7, 2017 Category: Biochemistry Authors: Luo Y, Meng X, Zhou P, Lu S, Qin M, Xu X, Sun G, Sun X Tags: Biochim Biophys Acta Source Type: research
Knockdown of sestrin2 increases pro-inflammatory reactions and ER stress in the endothelium via an AMPK dependent mechanism.
CONCLUSION: Knockdown of sesn2 aggravates atherosclerotic processes by increasing pro-inflammatory reactions and ER stress in the endothelium via an AMPK-dependent mechanism, suggesting that sesn2 might be a novel therapeutic target for atherosclerosis.
PMID: 28215577 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - February 15, 2017 Category: Biochemistry Authors: Hwang HJ, Jung TW, Choi JH, Lee HJ, Chung HS, Seo JA, Kim SG, Kim NH, Choi KM, Choi DS, Baik SH, Yoo HJ Tags: Biochim Biophys Acta Source Type: research
Insulin increases filtration barrier permeability via TRPC6-dependent activation of PKGI α signaling pathways.
Insulin increases filtration barrier permeability via TRPC6-dependent activation of PKGIα signaling pathways.
Biochim Biophys Acta. 2017 Mar 02;:
Authors: Rogacka D, Audzeyenka I, Rachubik P, Rychłowski M, Kasztan M, Jankowski M, Angielski S, Piwkowska A
Abstract
Podocytes are dynamic polarized cells on the surface of glomerular capillaries and an essential component of the glomerular filtration barrier. Insulin increases the activation of protein kinase G type Iα (PKGIα) subunits, leading to podocyte dysfunction. In addition, accumulating evidence suggests that TRPC6 channels are crucial mediator...
Source: Biochimica et Biophysica Acta - March 1, 2017 Category: Biochemistry Authors: Rogacka D, Audzeyenka I, Rachubik P, Rychłowski M, Kasztan M, Jankowski M, Angielski S, Piwkowska A Tags: Biochim Biophys Acta Source Type: research
