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Source: Biochimica et Biophysica Acta
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Total 177 results found since Jan 2013.
Phosphatidylethanolamine binding protein 4 (PEBP4) is a secreted protein and has multiple functions.
Abstract
Phosphatidylethanolamine binding proteins (PEBP) represent a superfamily of proteins that are conserved from bacteria to humans. In mammals, four members have been identified, PEBP1-4. To determine the functional differences among PEBP1-4 and the underlying mechanism for their actions, we performed a sequence alignment and found that PEBP4 contains a signal peptide and potential glycosylation sites, whereas PEBP1-3 are intracellular proteins. To test if PEBP4 is secreted, we made constructs with Myc epitope at the amino (N) terminus or carboxyl (C) terminus to mask the signal sequence or keep it free, res...
Source: Biochimica et Biophysica Acta - March 27, 2016 Category: Biochemistry Authors: He H, Liu D, Lin H, Jiang S, Ying Y, Chun S, Deng H, Zaia J, Wen R, Luo Z Tags: Biochim Biophys Acta Source Type: research
Ganglioside GM2 mediates migration of tumor cells by interacting with integrin and modulating the downstream signaling pathway.
Abstract
The definitive role of ganglioside GM2 in mediating tumor-induced growth and progression is still unknown. Here we report a novel role of ganglioside GM2 in mediating tumor cell migration and uncovered its mechanism. Data shows differential expression levels of GM2-synthase as well as GM2 in different human cancer cells. siRNA mediated knockdown of GM2-synthase in CCF52, A549 and SK-RC-26B cells resulted in significant inhibition of tumor cell migration as well as invasion in vitro without affecting cellular proliferation. Over-expression of GM2-synthase in low-GM2 expressing SK-RC-45 cells resulted in a ...
Source: Biochimica et Biophysica Acta - April 7, 2016 Category: Biochemistry Authors: Kundu M, Mahata B, Banerjee A, Chakraborty S, Debnath S, Ray SS, Ghosh Z, Biswas K Tags: Biochim Biophys Acta Source Type: research
Reducing VDAC1 expression induces a non-apoptotic role for pro-apoptotic proteins in cancer cell differentiation.
Abstract
Proteins initially identified as essential for apoptosis also mediate a wide range of non-apoptotic functions that include cell cycle progression, differentiation and metabolism. As this phenomenon was mostly reported with non-cancer cells, we considered non-conventional roles for the apoptotic machinery in the cancer setting. We found that treating glioblastoma (GBM) tumors with siRNA against VDAC1, a mitochondrial protein found at the crossroads of metabolic and survival pathways and involved in apoptosis, inhibited tumor growth while leading to differentiation of tumor cells into neuronal-like cells, a...
Source: Biochimica et Biophysica Acta - April 10, 2016 Category: Biochemistry Authors: Arif T, Krelin Y, Shoshan-Barmatz V Tags: Biochim Biophys Acta Source Type: research
D-3-deoxy-dioctanoylphosphatidylinositol Induces Cytotoxicity in Human MCF-7 Breast Cancer Cells via a Mechanism that Involves Downregulation of the D-type Cyclin-Retinoblastoma Pathway.
Abstract
Phosphatidylinositol analogs (PIAs) were originally designed to bind competitively to the Akt PH domain and prevent membrane translocation and activation. D-3-deoxy-dioctanoylphosphatidylinositol (D-3-deoxy-diC8PI), but not compounds with altered inositol stereochemistry (e.g., L-3-deoxy-diC8PI and L-3,5-dideoxy-diC8PI), is cytotoxic. However, high resolution NMR field cycling relaxometry shows that both cytotoxic and non-toxic PIAs bind to the Akt1 PH domain at the site occupied by the cytotoxic alkylphospholipid perifosine. This suggests another mechanism for cytotoxicity must account for the difference...
Source: Biochimica et Biophysica Acta - September 2, 2016 Category: Biochemistry Authors: Gradziel CS, Jordan PA, Jewel D, Dufort FJ, Miller SJ, Chiles TC, Roberts MF Tags: Biochim Biophys Acta Source Type: research
Ouabain interactions with the α4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatogenic cells.
Abstract
In addition to the ubiquitous α1 isoform of the sodium pump, sperm cells also express a male-specific α4 isoform whose function has been associated with sperm motility, fertility, and capacitation. Here we investigate in the murine spermatogenic cell line GC-2 interactions of the α4 isoform with the cardiotonic steroid ouabain in signaling cascades involved in the non-classical action of steroid hormones. Exposure of GC-2 cells to low concentrations of ouabain stimulates the phosphorylation of Erk1/2 and of the transcription factors CREB and ATF-1. As a consequence of this signaling cascade, ouabain st...
Source: Biochimica et Biophysica Acta - September 2, 2016 Category: Biochemistry Authors: Upmanyu N, Dietze R, Kirch U, Scheiner-Bobis G Tags: Biochim Biophys Acta Source Type: research
S-nitrosylation of peroxiredoxin 1 contributes to viability of lung epithelial cells during Bacillus anthracis infection.
CONCLUSIONS: Anthrax infection results in S-nitrosylation of multiple host proteins, including Prx1. The nitrosylation-dependent decrease in peroxidase activity of Prx1 and increase in its chaperone activity is one factor contributing to enhancing infected cell viability.
GENERAL SIGNIFICANCE: These results provide a new venue of mechanistic investigation for inhalational anthrax that could lead to novel and potentially effective countermeasures.
PMID: 27612662 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - September 6, 2016 Category: Biochemistry Authors: Chung MC, Alem F, Hamer SG, Narayanan A, Shatalin K, Bailey C, Nudler E, Hakami RM Tags: Biochim Biophys Acta Source Type: research
Upregulation of the S1P3 receptor in metastatic breast cancer cells increases migration and invasion by induction of PGE2 and EP2/EP4 activation.
Abstract
Breast cancer is one of the most common and devastating malignancies among women worldwide. Recent evidence suggests that malignant progression is also driven by processes involving the sphingolipid molecule sphingosine 1-phoshate (S1P) and its binding to cognate receptor subtypes on the cell surface. To investigate the effect of this interaction on the metastatic phenotype, we used the breast cancer cell line MDA-MB-231 and the sublines 4175 and 1833 derived from lung and bone metastases in nude mice, respectively. In both metastatic cell lines expression of the S1P3 receptor was strongly upregulated com...
Source: Biochimica et Biophysica Acta - September 7, 2016 Category: Biochemistry Authors: Filipenko I, Schwalm S, Reali L, Pfeilschifter J, Fabbro D, Huwiler A, Zangemeister-Wittke U Tags: Biochim Biophys Acta Source Type: research
Role of CCL20 mediated immune cell recruitment in NF- κB mediated TRAIL resistance of pancreatic cancer.
In conclusion, our data show a functional role of a RelA-CCL20 pathway in PDAC TRAIL resistance. We demonstrate how the therapy-induced cross-talk of cancer cells with immune cells affects treatment responses, knowledge needed to tailor novel bi-specific treatments, which target tumor cell as well as immune cells.
PMID: 28188806 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - February 6, 2017 Category: Biochemistry Authors: Geismann C, Grohmann F, Dreher A, Häsler R, Rosenstiel P, Legler K, Hauser C, Egberts JH, Sipos B, Schreiber S, Linkermann A, Hassan Z, Schneider G, Schäfer H, Arlt A Tags: Biochim Biophys Acta Source Type: research
Glucose-derived AGEs promote migration and invasion of colorectal cancer by up-regulating Sp1 expression.
In conclusion, glucose-derived AGEs promote the invasion and metastasis of CRC partially through the RAGE/ERK/SP1/MMP2 cascade. These findings may provide an explanation for the poor prognoses of colorectal cancer in diabetic patients.
PMID: 28237576 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - February 21, 2017 Category: Biochemistry Authors: Deng R, Wu H, Ran H, Kong X, Hu L, Wang X, Su Q Tags: Biochim Biophys Acta Source Type: research
Astaxanthin attenuated pressure overload-induced cardiac dysfunction and myocardial fibrosis: Partially by activating SIRT1.
CONCLUSIONS: Our data demonstrate that AST improves cardiac function and attenuates fibrosis by decreasing phosphorylation and deacetylation of R-SMADs. SIRT1 contributes to AST's protective function by reducing acetylation of R-SMADs.
GENERAL SIGNIFICANCE: These data suggest that AST may be useful as a preventive/therapeutic agent for cardiac dysfunction and myocardial fibrosis.
PMID: 28300638 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - March 10, 2017 Category: Biochemistry Authors: Zhang J, Wang QZ, Zhao SH, Ji X, Qiu J, Wang J, Zhou Y, Cai Q, Zhang J, Gao HQ Tags: Biochim Biophys Acta Source Type: research
Implications of cerebrovascular ATP-binding cassette transporter G1 (ABCG1) and apolipoprotein M in cholesterol transport at the blood-brain barrier.
Abstract
Impaired cholesterol/lipoprotein metabolism is linked to neurodegenerative diseases such as Alzheimer's disease (AD). Cerebral cholesterol homeostasis is maintained by the highly efficient blood-brain barrier (BBB) and flux of the oxysterols 24(S)-hydroxycholesterol and 27-hydroxycholesterol, potent liver-X-receptor (LXR) activators. HDL and their apolipoproteins are crucial for cerebral lipid transfer, and loss of ATP binding cassette transporters (ABC)G1 and G4 results in toxic accumulation of oxysterols in the brain. The HDL-associated apolipoprotein (apo)M is positively correlated with pre-β HDL form...
Source: Biochimica et Biophysica Acta - March 13, 2017 Category: Biochemistry Authors: Kober AC, Manavalan AP, Tam-Amersdorfer C, Holmér A, Saeed A, Danesh EF, Zandl M, Albrecher NM, Björkhem I, Kostner GM, Dahlbäck B, Panzenboeck U Tags: Biochim Biophys Acta Source Type: research
Identity and function of a cardiac mitochondrial small conductance Ca(2+)-activated K(+) channel splice variant.
Abstract
We provide evidence for location and function of a small conductance, Ca(2+)-activated K(+) (SKCa) channel isoform 3 (SK3) in mitochondria (m) of guinea pig, rat and human ventricular myocytes. SKCa agonists protected isolated hearts and mitochondria against ischemia/reperfusion (IR) injury; SKCa antagonists worsened IR injury. Intravenous infusion of a SKCa channel agonist/antagonist, respectively, in intact rats was effective in reducing/enhancing regional infarct size induced by coronary artery occlusion. Localization of SK3 in mitochondria was evidenced by Western blot of inner mitochondrial membrane,...
Source: Biochimica et Biophysica Acta - March 22, 2017 Category: Biochemistry Authors: Yang M, Camara AK, Aldakkak M, Kwok WM, Stowe DF Tags: Biochim Biophys Acta Source Type: research
Identification of a molecular signaling gene-gene regulatory network between GWAS susceptibility genes ADTRP and MIA3/TANGO1 for coronary artery disease.
In conclusion, we have uncovered a novel molecular signaling pathway for the pathogenesis of CAD, which involves a novel gene-gene regulatory network. We show that ADTRP positively regulates PIK3R3 expression, which leads to activation of AKT and up-regulation of MIA3/TANGO1, thereby regulating endothelial cell functions directly relevant to atherosclerosis.
PMID: 28341552 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - March 21, 2017 Category: Biochemistry Authors: Luo C, Wang F, Ren X, Ke T, Xu C, Tang B, Qin S, Yao Y, Chen Q, Wang QK Tags: Biochim Biophys Acta Source Type: research
Neurotoxic mechanisms by which the USP14 inhibitor IU1 depletes ubiquitinated proteins and Tau in rat cerebral cortical neurons: relevance to Alzheimer's disease.
In conclusion, pharmacologically inhibiting (with low or high IU1 concentrations) or genetically down-regulating USP14 fail to enhance proteasomal degradation of Ub-proteins or Tau in neurons.
PMID: 28372990 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - March 31, 2017 Category: Biochemistry Authors: Kiprowska MJ, Stepanova A, Todaro DR, Galkin A, Haas A, Wilson SM, Figueiredo-Pereira ME Tags: Biochim Biophys Acta Source Type: research
Loss of DUSP3 activity radiosensitizes human tumor cell lines via attenuation of DNA repair pathways.
CONCLUSION: The loss of DUSP3 activity markedly increases gamma radiation-induced DNA strand breaks, suggesting a potential novel role for DUSP3 in DNA repair.
GENERAL SIGNIFICANCE: The radioresistance of tumor cells is effectively reduced by a combination of approaches through the inhibition of DUSPs.
PMID: 28389334 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - April 4, 2017 Category: Biochemistry Authors: Torres TE, Russo LC, Santos A, Marques GR, Magalhaes YT, Tabassum S, Forti FL Tags: Biochim Biophys Acta Source Type: research
