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Nrf2/PINK1-mediated mitophagy induction alleviates sodium fluoride-induced hepatic injury by improving mitochondrial function, oxidative stress, and inflammation
Ecotoxicol Environ Saf. 2023 Feb 13;252:114646. doi: 10.1016/j.ecoenv.2023.114646. Online ahead of print.ABSTRACTMitophagy has distinct functions, which can lead to either protection or damage of tissues. Though current evidence indicated that NaF triggers mitophagy, the role and regulation of mitophagy in sodium fluoride (NaF)-induced liver injury still remain unclear. Therefore, we exployed the cell and mouse models and confirmed that NaF treatment activates mitophagy. Knocking down PTEN-induced putative kinase protein 1 (PINK1) expression attenuated mitophagy and increased the degree of mitochondrial impairment, oxidati...
Source: Ecotoxicology and Environmental Safety - February 15, 2023 Category: Environmental Health Authors: Chao Song Aiguo Zhang Man Zhang Yuzhen Song Heping Huangfu Shuangxing Jin Yanting Sun Chunhui Zhang Dongmei Shi Jundong Wang Wei Peng Qin Luo Source Type: research

Sodium fluoride activates the extrinsic apoptosis via regulating NOX4/ROS-mediated p53/DR5 signaling pathway in lung cells both in vitro and in vivo
In this study, we report that death receptor 5 (DR5), a major component of the extrinsic apoptotic pathway, is markedly induced upon NaF stimulation. Enhanced DR5 induction was necessary for the apoptotic effects of NaF, inasmuch as transfected BEAS-2B cells with DR5 siRNA attenuated NaF-induced caspase-8 activation in lung cells. Mechanism investigation indicated that the induction of DR5, following NaF exposure, was mediated by tumor protein 53 (p53)-dependent transcriptional activation. Notably, we demonstrated that NaF could induce a significant increase in intracellular reactive oxygen species (ROS) level derived from...
Source: Free Radical Biology and Medicine - April 15, 2021 Category: Biology Authors: Chao Song Dongmei Shi Kaiwen Chang Xianghui Li Qing Dong Xia Ma Xuefei Wang Zhenhuan Guo Yonglu Liu Jundong Wang Source Type: research

AMPK/p38/Nrf2 activation as a protective feedback to restrain oxidative stress and inflammation in microglia stimulated with sodium fluoride.
In conclusion, these data demonstrated for the first time that Nrf2 activation exerts a neuroprotective effect on NaF-stimulated redox imbalance and inflammation that is dependent on the AMPK/p38 pathway. PMID: 31837563 [PubMed - indexed for MEDLINE]
Source: Chemosphere - March 20, 2020 Category: Chemistry Authors: Song C, Heping H, Shen Y, Jin S, Li D, Zhang A, Ren X, Wang K, Zhang L, Wang J, Shi D Tags: Chemosphere Source Type: research

Vitamin C Attenuates Sodium Fluoride-Induced Mitochondrial Oxidative Stress and Apoptosis via Sirt1-SOD2 Pathway in F9 Cells.
Abstract Increasing evidence has suggested an important role played by reactive oxygen species (ROS) in the pathogenesis of fluorosis. Accumulating evidence demonstrates that vitamin C administration ameliorate sodium fluoride (NaF)-induced oxidative stress. However, the potentially beneficial effects of vitamin C against NaF-induced cytotoxicity and the underlying molecular mechanisms of this protection are not fully understood. Here, we found that NaF stimulated cytotoxicity, increased mitochondrial reactive oxygen species (mROS) production, and induced apoptosis in F9 embryonic carcinoma cells. Consistent with ...
Source: Biological Trace Element Research - December 18, 2018 Category: Biology Authors: Peng W, Xu S, Zhang J, Zhang Y Tags: Biol Trace Elem Res Source Type: research

TGF- β1 acts as mediator in fluoride-induced autophagy in the mouse osteoblast cells.
TGF-β1 acts as mediator in fluoride-induced autophagy in the mouse osteoblast cells. Food Chem Toxicol. 2018 Mar 02;: Authors: Zhao Y, Li Y, Gao Y, Yuan M, Manthari RK, Wang J, Wang J Abstract It is well known that excess fluoride intake can result in fluorosis, which is a serious public health problem. TGF-β1 affects a wide variety of cellular activities and plays an important role in fluorosis. Recent literature proved that fluoride induces autophagy, however, the mechanism is still unclear, and the role of TGF-β1 in the fluoride-induced autophagy should be further illustrated. Therefore, in this...
Source: Food and Chemical Toxicology - March 2, 2018 Category: Food Science Authors: Zhao Y, Li Y, Gao Y, Yuan M, Manthari RK, Wang J, Wang J Tags: Food Chem Toxicol Source Type: research

Micromolar Levels of Sodium Fluoride Promote Osteoblast Differentiation Through Runx2 Signaling.
In this study, we characterized the effects of micromolar levels of NaF on the proliferation and osteogenic differentiation of MC3T3-E1 osteoblastic cells. NaF significantly enhanced the proliferation, alkaline phosphatase (ALP) activity, and mineralization of MC3T3-E1 cells. Quantitative real-time PCR analysis revealed that the expression of mRNAs encoding runt-related transcription factor 2 (Runx2), Osterix, Osteopontin and Osteocalcin was up-regulated in NaF-treated MC3T3-E1 cells compared with untreated controls. Western blot analysis demonstrated that Runx2 and Osterix were inhibited by Runx2 siRNA but were re-activat...
Source: Biological Trace Element Research - January 12, 2017 Category: Biology Authors: Lee M, Arikawa K, Nagahama F Tags: Biol Trace Elem Res Source Type: research

Differential NF-κB and MAPK activation underlies fluoride- and TPA-mediated CXCL8 (IL-8) induction in lung epithelial cells
In conclusion, all MAPK cascades were involved in NaF-induced CXCL8 release, whereas only ERK1/2 activation was involved in response to TPA. Furthermore, NF-κB activation appeared to be indispensable for CXCL8 induction. The early response, magnitude, and persistency of MAPK and NF-κB signaling seemed to be critical determinants for the potential to induce CXCL8. These findings underscore that a strong, rapid, and relatively transient activation of ERK1/2 in combination with NF-kB may be sufficient for a strong induction of CXCL8, which may exceed the effects of a more moderate ERK1/2 activation in combination ...
Source: Journal of Inflammation Research - December 12, 2014 Category: Allergy & Immunology Tags: Journal of Inflammation Research Source Type: research

Wnt-RhoA Signaling Pathways in Fluoride-Treated Ameloblast-Lineage Cells
This study examined the effect of sodium fluoride (NaF) on the Wnt and RhoA signaling pathways in murine ameloblast-lineage cells (ALCs) to better understand the developmental mechanisms of dental fluorosis. Wnt and Rho pathway activities were investigated when ALCs were treated with 1.5 mM NaF, dickkopf-related protein-1 (Dkk-1), secreted frizzled related-protein-2 (sFRP-2), β-catenin siRNA dominant negative RhoA (RhoADN) plasmid and Y-27632. Wnt pathway activity was investigated via RT-PCR, Western blot and Topflash luciferase assay. The activity of the RhoA pathway was analyzed via Rho pull-down assay and immunoprecipi...
Source: Cells Tissues Organs - November 14, 2014 Category: Cytology Source Type: research