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Molecules, Vol. 26, Pages 2610: In Vitro Inhibitory Analysis of Rationally Designed siRNAs against MERS-CoV Replication in Huh7 Cells
In this report, we have discussed the prediction, designing, and evaluation of potential siRNA targeting the ORF1ab gene for the inhibition of MERS-CoV replication. The online software, siDirect 2.0 was used to predict and design the siRNAs, their secondary structure and their target accessibility. ORF1ab gene folding was performed by RNAxs and RNAfold software. A total of twenty-one siRNAs were selected from 462 siRNAs according to their scoring and specificity. siRNAs were evaluated in vitro for their cytotoxicity and antiviral efficacy in Huh7 cell line. No significant cytotoxicity was observed for all siRNAs in Huh7 ce...
Source: Molecules - April 29, 2021 Category: Chemistry Authors: Sherif Aly El-Kafrawy Sayed Sartaj Sohrab Zeenat Mirza Ahmed M. Hassan Fatima Alsaqaf Esam Ibraheem Azhar Tags: Article Source Type: research

LncRNA NEAT1 modulates sorafenib resistance in hepatocellular carcinoma through regulating the miR-149-5p/AKT1 axis
Conclusions: NEAT1/miR-149-5p/AKT1 pathway-based therapy might be a potential clinical application for HCC patients.
Source: The Saudi Journal of Gastroenterology - July 28, 2020 Category: Gastroenterology Authors: Yuexiang Niu Gongen Tang Xiuli Wu Chaoyu Wu Source Type: research

A Newly Synthesized platinum-based Compound (PBC-II) Increases Chemosensitivity of HeLa Ovarian Cancer Cells Via Inhibition of Autophagy
Publication date: Available online 16 October 2019Source: Saudi Pharmaceutical JournalAuthor(s): Moureq Rashed Alotaibi, Homood Moqbel As Sobeai, Faten Abdullah Alaqil, Mashal Almutairi, Khalid Alhazzani, Adam A.A. Sulaiman, Anvarhusein A. Isab, Nasser Hadal AlotaibiAbstractthere are many mechanisms of resistance, chemoresistance of HeLa cells to anti-cancer agents seems to be autophagy-mediated. While using very effective anti-cancers such as Doxorubicin and cisplatin, cells overcome the cytotoxicity of these drugs through promotion of what so-called cytoprotective autophagy. Here in this study, we sought to introduce a n...
Source: Saudi Pharmaceutical Journal - October 17, 2019 Category: Drugs & Pharmacology Source Type: research

Identification of FBXW7 α-regulated genes in M1-polarized macrophages in colorectal cancer by RNA sequencing.
CONCLUSION: These results prove that the FBXW7α/miR-205 axis plays an important role in TAM polarization and could facilitate further exploration of its molecular mechanism. PMID: 31423512 [PubMed - in process]
Source: Saudi Medical Journal - August 20, 2019 Category: Middle East Health Tags: Saudi Med J Source Type: research

miRNA-21 Inhibition suppresses the human epithelial ovarian cancer by targeting PTEN signal pathway
ConclusionThe suppression of microRNA-21 inhibits the progression of EOC profoundly. In EOC, miR-21 is negatively correlated with the expression of PTEN gene.
Source: Saudi Journal of Biological Sciences - August 10, 2019 Category: Biology Source Type: research

Graphene Quantum Dots: Synthesis, Characterization, Cell Viability, Genotoxicity for Biomedical Applications
We report the synthesis and applications of a novel N-doped graphene quantum dots (GQDs) using hydrothermal reaction between citric acid and p-aminophenol. The synthesized N-doped GQDs have been characterized physico-chemically and evaluated its antioxidant, antimicrobial, DNA binding and cleavage activities. siRNA loading studies were performed and their effects on cells were evaluated. Obtained results indicate that monodisperse solution of N-doped GQDs has been obtained with particles size ca. ∼10.9±1.3 nm. UV-Vis spectroscopy studies of the interactions between the N-doped GQDs and calf thymus DNA (CT-DNA) showed th...
Source: Saudi Pharmaceutical Journal - May 21, 2019 Category: Drugs & Pharmacology Source Type: research

STAT3-siRNA induced B16.F10 melanoma cell death: more association with VEGF downregulation than p-STAT3 knockdown
Publication date: Available online 30 May 2018Source: Saudi Pharmaceutical JournalAuthor(s): Aws AlshamsanAbstractSTAT3 knockdown by small interfering RNA (siRNA) has been described to inhibit carcinogenic growth in various types of tumors. Earlier we have reported delivery of siRNA by oleic acid- and stearic acid-modified-polyethylenimine and enhancement of silencing of STAT3 by small interfering RNA (siRNA) in B16.F10 melanoma cell lines and consequent tumor suppression. Present investigation mainly focused on the downstream events involved in B16.F10 melanoma cell death and consequent tumor suppression following knockdo...
Source: Saudi Pharmaceutical Journal - July 10, 2018 Category: Drugs & Pharmacology Source Type: research

STAT3-siRNA induced B16.F10 melanoma cell death: Association with VEGF downregulation than p-STAT3 knockdown
Publication date: Available online 30 May 2018 Source:Saudi Pharmaceutical Journal Author(s): Aws Alshamsan STAT3 knockdown by small interfering RNA (siRNA) has been described to inhibit carcinogenic growth in various types of tumors. Earlier we have reported delivery of siRNA by oleic acid- and stearic acid-modified-polyethylenimine and enhancement of silencing of STAT3 by small interfering RNA (siRNA) in B16.F10 melanoma cell lines and consequent tumor suppression. Present investigation mainly focused on the downstream events involved in B16.F10 melanoma cell death and consequent tumor suppression following knockdown of...
Source: Saudi Pharmaceutical Journal - May 31, 2018 Category: Drugs & Pharmacology Source Type: research

FoxM1 is an independent poor prognostic marker and therapeutic target for advanced Middle Eastern breast cancer.
In this study, we explored the prevalence and clinical implication of FoxM1 overexpression in Saudi breast cancer. FoxM1 protein overexpression was seen in 79% (770/975) of BC tissues and was associated with aggressive clinical parameters such as younger age (< 30 yrs) (p = 0.0172), high grade (p < 0.0001), mucinous histology (p < 0.0001) and triple negative phenotype (p < 0.0001). Overexpression of FoxM1 was significantly associated with activated AKT (p < 0.0001), Ki67 expression (p < 0.0001), VEGF (p < 0.0001), MMP-9 (p < 0.0001), XIAP (p < 0.0001) and Bcl-xL (p = 0.0300). Importantly, FoxM1 o...
Source: Oncotarget - May 2, 2018 Category: Cancer & Oncology Tags: Oncotarget Source Type: research