STAT3-siRNA induced B16.F10 melanoma cell death: more association with VEGF downregulation than p-STAT3 knockdown

Publication date: Available online 30 May 2018Source: Saudi Pharmaceutical JournalAuthor(s): Aws AlshamsanAbstractSTAT3 knockdown by small interfering RNA (siRNA) has been described to inhibit carcinogenic growth in various types of tumors. Earlier we have reported delivery of siRNA by oleic acid- and stearic acid-modified-polyethylenimine and enhancement of silencing of STAT3 by small interfering RNA (siRNA) in B16.F10 melanoma cell lines and consequent tumor suppression. Present investigation mainly focused on the downstream events involved in B16.F10 melanoma cell death and consequent tumor suppression following knockdown of p-STAT3 by siRNA. Lipid-substituted polyethylenimine (PEI)-p-STAT3-siRNA were prepared and characterized by measuring its N/P ratio, zeta potential, size, association and dissociation with siRNA. B16.F10 melanoma cells were treated with six different concentrations of PEI-p-STAT3-siRNA (200, 100, 50, 25, 12.5 and 6.25 nM). Downregulation of p-STAT3 and VEGF were studied using western blot and ELISA in association with the melanoma cell death. PEI-p-STAT3-siRNA hydrodynamic diameter ranged from 110 to 270 nm. PEI assisted p-STAT3-siRNA delivery exhibited increased uptake by B16.F10, when analyzed by fluorescent and confocal microscopy along with flowcytometry. It induced concentration-dependent knockdown of the p-STAT3 that also downregulated VEGF expression in similar fashion and induced B16.F10 cell death. Higher concentrations of p-STAT3-siRNA ap...
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research

Related Links:

Viviana Anelli, Anita Ordas, Susanne Kneitz, Leonel Munoz Sagredo, Victor Gourain, Manfred Schartl, Annemarie H. Meijer, Marina Mione
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
Conclusion: LE with adjuvant radiation seems to offer good locoregional control without reducing the survival and may be an option of treatment for patients with small, superficial AM. However, APR should be offered for patients with locally advanced disease or as a salvage following recurrence.
Source: Nigerian Journal of Clinical Practice - Category: Rural Health Authors: Source Type: research
Optical coherence tomography (OCT) is an imaging technique that allows ophthalmologists to view the eye’s blood vessels, to study the structure of tissues in various clinical and scientific applications. Though this technology is already widely...
Source: Medgadget - Category: Medical Devices Authors: Tags: Diagnostics Materials Medicine Nanomedicine Oncology Radiology Source Type: blogs
Researchers at the UA Cancer Center hope early-phase clinical trials will lead to new treatment options for melanoma patients with BRAF or MEK mutations.
Source: The University of Arizona: Health - Category: Universities & Medical Training Authors: Source Type: research
Discussion Skin color is primarily due to genetic factors. Melanocytes are usually found in skin in the basal layer of the epidermis which is also where the melanin usually lies. There are a range of normal skin phenotypes that have been described by Fitzpatrick and range from I-VI: I – pale white skin, blond hair, blue eyed, does not tan, always burns II – fair skin, blue eyed, tans poorly, burns easily III – darker white skin, burns initially then tans IV – light brown skin, tans easily, burns minimally V – brown skin, tans darkly easily, burns rarely VI – dark brown skin, alway...
Source: - Category: Pediatrics Authors: Tags: Uncategorized Source Type: news
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Letters to the Editor Source Type: research
Here are a few I have come across the last week or so. Note: Each link is followed by a title and a few paragraphs. For the full article click on the link above title of the article. Note also that full access to some links may require site registration or subscription payment.General CommentWell the Silly Season has arrived and it is time to have a break. Barring exciting news this will be the last full week with the last regular post on the 22nd December.Enjoy!----- the apps: automated instruments 'no good for melanoma' Doctors w...
Source: Australian Health Information Technology - Category: Information Technology Authors: Source Type: blogs
Combination therapy with BRAF and MEK inhibitors significantly improves survival in BRAF mutated melanoma patients but is unable to prevent disease recurrence due to the emergence of drug resistance. Cancer st...
Source: Journal of Experimental and Clinical Cancer Research - Category: Cancer & Oncology Authors: Tags: Research Source Type: research
Ocular melanoma is a rare but often deadly malignancy that arises in the uvea (commonest primary site), conjunctiva or the orbit. Primary orbital melanoma (POM) is exceedingly rare, with approximately 60 cases...
Source: BMC Cancer - Category: Cancer & Oncology Authors: Tags: Research article Source Type: research
AbstractLynch syndrome (LS) is an autosomal-dominant inherited disorder characterized by a predisposition to colorectal cancer and extracolonic cancers (particularly endometrium, ovary, stomach, small bowel, hepatobiliary tract, pancreas, urothelial tract, brain, and skin). Muir –Torre syndrome (MTS) is considered a phenotypical variant of LS, where patients develop sebaceous neoplasms and keratoacanthomas. Currently, only few studies and case reports suggest an association between LS and other skin cancers, such as Bowens’ disease, melanoma and squamous cell carcinoma (SCC). In this case-report we describe the...
Source: Familial Cancer - Category: Cancer & Oncology Source Type: research
More News: Cancer & Oncology | Melanoma | Middle East Health | Saudi Arabia Health | Skin Cancer