A Newly Synthesized platinum-based Compound (PBC-II) Increases Chemosensitivity of HeLa Ovarian Cancer Cells Via Inhibition of Autophagy

Publication date: Available online 16 October 2019Source: Saudi Pharmaceutical JournalAuthor(s): Moureq Rashed Alotaibi, Homood Moqbel As Sobeai, Faten Abdullah Alaqil, Mashal Almutairi, Khalid Alhazzani, Adam A.A. Sulaiman, Anvarhusein A. Isab, Nasser Hadal AlotaibiAbstractthere are many mechanisms of resistance, chemoresistance of HeLa cells to anti-cancer agents seems to be autophagy-mediated. While using very effective anti-cancers such as Doxorubicin and cisplatin, cells overcome the cytotoxicity of these drugs through promotion of what so-called cytoprotective autophagy. Here in this study, we sought to introduce a novel platinum-based compound PBC-II that possesses anti-cancer activity. Our data showed that PBC-II is able to induce apoptosis at relatively low concentrations, with no detectable reactive oxygen species (ROS). However, further experiments demonstrated that exposure of HeLa cells to PBC-II did not promote autophagy; rather, it resulted in accumulation of p62 and decrease in LC3-II levels. Autophagy was then promoted in HeLa cells pharmacologically by Doxorubicin and genetically by siRNA IL-10. In order to confirm promotion of autophagy in our model, we performed acridine orange staining to assess for autophagy under microscope as well as via flow cytometry. We then measured protein level of autophagy markers p62 and LC3 by western blot. Our data indicated that PBC-II interferes with therapy-induced autophagy. We also determined PI3K activity while co-incub...
Source: Saudi Pharmaceutical Journal - Category: Drugs & Pharmacology Source Type: research

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Published on 2019-11-18 14:39:19
Source: Journal of the Belgian Society of Radiology - Category: Radiology Source Type: research
Authors: Moioli M, Barra F, Maramai M, Valenzano Menada M, Vellone VG, Costantini S, Ferrero S PMID: 31726889 [PubMed - as supplied by publisher]
Source: Expert Opinion on Investigational Drugs - Category: Drugs & Pharmacology Tags: Expert Opin Investig Drugs Source Type: research
A new report reveals Johnson &Johnson rushed the test results of its iconic baby powder to show it was free of asbestos contamination. The Wall Street Journal reported Sunday that two independent laboratories found no evidence of asbestos in the same recalled lot of baby powder used in the U.S Food and Drug Administration report last month. But evidence shows the quick turnaround on the tests may have impacted results. One of those laboratories, commissioned by J&J after the FDA report, initially found asbestos in the talc sample, but quickly recanted. It told J&J the sample had been contaminated by the air-conditio...
Source: Asbestos and Mesothelioma News - Category: Environmental Health Authors: Source Type: news
Gynecologic cancers impact women of all ages. Some women may wish to preserve their capacity for future childbearing. With appropriate patient selection, acceptable oncologic outcomes may be achieved with preservation of fertility. Determination of eligibility for fertility preservation is guided by patient factors, tumor histology, and preoperative local staging with pelvic MR imaging. The aim of this article is to educate radiologists on the current guidelines for fertility-sparing techniques in women with early stage cervical, endometrial, and ovarian malignancies.
Source: Radiologic Clinics of North America - Category: Radiology Authors: Source Type: research
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Source: Clinical Journal of Oncology Nursing - Category: Nursing Authors: Tags: Clin J Oncol Nurs Source Type: research
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Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
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Source: Journal of Industrial and Engineering Chemistry - Category: Chemistry Source Type: research
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Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
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Source: World Journal of Surgical Oncology - Category: Cancer & Oncology Authors: Tags: Research Source Type: research
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Source: Medical Oncology - Category: Cancer & Oncology Source Type: research
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