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Effects of the lncRNA ENST00000623984 on colon cancer and the biological characteristics of colon cancer cells
In this study, the expression levels of ENST000000623984 were first examined in tumor tissue and adjacent normal tissue from 40 patients with colorectal cancer and LoVo cells using quantitative real-time PCR. By siRNA transfection, ENST00000623984 expression was knocked down. Using flow cytometry, cell cycle progression and cell viability were examined in basal and knockdown LoVo cells. The CCK-8 assay was used to assess the cell proliferation rate, and the Transwell assay was used to determine the migration and invasion abilities. The ENST000000623984 expression level was increased in colorectal cancer. Knockdown of ENST0...
Source: European Journal of Histochemistry - July 2, 2021 Category: Biomedical Science Authors: Zhi-bao Liu, Jing-hua Zhang, Jing-hua Gao, Jian Shi Source Type: research

Targeting LncRNA EPIC1 to inhibit human colon cancer cell progression.
Abstract Long non-coding RNA EPIC1 (Lnc-EPIC1) binds MYC protein, which is essential for MYC function and expression of MYC target genes. The current study tested its expression and potential functions in human colon cancer cells. We show that Lnc-EPIC1 expression is elevated in human colon cancer tissues and primary human colon cancer cells. Whereas its expression is relatively low in normal colon tissues and colon epithelial cells. In the primary human colon cancer cells, Lnc-EPIC1 siRNA largely inhibited cancer cell growth, proliferation, migration and invasion. Further, Lnc-EPIC1 silencing induced significant ...
Source: Aging - November 7, 2020 Category: Biomedical Science Authors: Wu Q, Wei J, Zhao C, Xiang S, Shi M, Wang Y Tags: Aging (Albany NY) Source Type: research

MLS128 antibody-induced suppression of colon cancer cell growth is mediated by a desmocollin and a 110 kDa glycoprotein.
Authors: Shuck SC, Hong T, Kalkum M, Igarashi R, Kajiya K, Termini J, Yamamoto K, Fujita-Yamaguchi Y Abstract Protein glycosylation is a diverse form of post-translational modification. Two to three consecutive O-linked N-acetylgalactosamines (Tn-antigens) are recognized by antibodies such as MLS128. MLS128 mAb inhibited cell growth and bound to a 110 kDa glycoprotein (GP) in LS180 and HT29 colon cancer cells. However, purification and identification of the 110 kDa GP was unsuccessful due to its low abundance. The present study used a highly sophisticated and sensitive mass spectrometry method to identify proteins ...
Source: BioScience Trends - June 8, 2019 Category: Biomedical Science Tags: Biosci Trends Source Type: research

A Mechanism for the Temporal Potentiation of Genipin to the Cytotoxicity of Cisplatin in Colon Cancer Cells.
CONCLUSION: The interaction of leaked electron with Cisplatin was important during ROS generation. Inhibition of UCP2-mediated proton leak with Genipin potentiated the cytotoxicity of Cisplatin. Owing to the compensatory effects against Genipin, shorter Genipin treatment before Cisplatin was recommended in order to achieve better potentiation effect. PMID: 27429587 [PubMed - in process]
Source: International Journal of Medical Sciences - July 20, 2016 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Targeted delivery of small interfering RNA to colon cancer cells using chitosan and PEGylated chitosan nanoparticles
Publication date: 20 August 2016 Source:Carbohydrate Polymers, Volume 147 Author(s): Walter E. Rudzinski, Adriana Palacios, Abuzar Ahmed, Michelle A. Lane, Tejraj M. Aminabhavi Small interfering RNA (siRNA) molecules specifically target messenger RNA species, decreasing intracellular protein levels. β-Catenin protein concentrations are increased in 70–80% of colon tumors, promoting tumor progression. Chitosan exhibits low levels of toxicity and can be transported across mucosal membranes; therefore, our objective was to develop chitosan and poly(ethylene glycol)-grafted (PEGylated) chitosan nanoparticles, 100–1...
Source: Carbohydrate Polymers - April 26, 2016 Category: Biomedical Science Source Type: research

Sodium Butyrate Induces Apoptosis of Human Colon Cancer Cells by Modulating ERK and Sphingosine Kinase 2.
CONCLUSION: ERK regulates the export of SphK2 and apoptosis of HCT116 cells by modulating PKD. Modulation of these molecules may help increase the sensitivity of colon cancer cells to the physiologic anti-colon cancer agent, NaBT. PMID: 24709100 [PubMed - in process]
Source: Biomedical and Environmental Sciences : BES - March 1, 2014 Category: Biomedical Science Authors: Xiao M, Liu YG, Zou MC, Zou F Tags: Biomed Environ Sci Source Type: research

TRPM5-mediated calcium uptake regulates mucin secretion from human colon goblet cells
Mucin 5AC (MUC5AC) is secreted by goblet cells of the respiratory tract and, surprisingly, also expressed de novo in mucus secreting cancer lines. siRNA-mediated knockdown of 7343 human gene products in a human colonic cancer goblet cell line (HT29-18N2) revealed new proteins, including a Ca2+-activated channel TRPM5, for MUC5AC secretion. TRPM5 was required for PMA and ATP-induced secretion of MUC5AC from the post-Golgi secretory granules. Stable knockdown of TRPM5 reduced a TRPM5-like current and ATP-mediated Ca2+ signal. ATP-induced MUC5AC secretion depended strongly on Ca2+ influx, which was markedly reduced in TRPM5 k...
Source: eLife - May 28, 2013 Category: Biomedical Science Authors: Mitrovic, S., Nogueira, C., Cantero-Recasens, G., Kiefer, K., Fernandez-Fernandez, J. M., Popoff, J.-F., Casano, L., Bard, F. A., Gomez, R., Valverde, M. A., Malhotra, V. Tags: Cell biology Source Type: research