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Specialty: Pathology
Cancer: Skin Cancer
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Total 45 results found since Jan 2013.
Silencing of SiX-4 enhances the chemosensitivity of melanoma cells to Cisplatin
In this study, we investigated whether silencing of SIX4 in a melanoma cell line (A375 cells) in combination with Cisplatin can affect the apoptosis and suppression of cell cycle progression, migration of the melanoma cells. MTT test and colony formation assay was applied to determine the IC50 of Cisplatin and the combined effect of SIX4 siRNA and Cisplatin on the viability and clonogenesis of the A-375 cells. qRT-PCR was performed to determine the c-myc, BCL-2, BAX, MMP-9, CXCR4, and Rock genes expression. Furthermore, flow cytometry was applied to evaluate apoptosis, autophagy, and the cell cycle status in different grou...
Source: Pathology, Research and Practice - November 12, 2022 Category: Pathology Authors: Aysan Fallah Vazirabad Saeed Noorolyai Elham Baghbani Soltanali Mahboob Felor Zargari Shima Rahmani Amin Sorkhabi Nooshin Montazami Pouriya Sameti Behzad Baradaran Source Type: research
Clear cell squamous cell carcinoma of the tongue exhibits characteristics as an undifferentiated squamous cell carcinoma
Pathol Res Pract. 2022 Apr 26;235:153909. doi: 10.1016/j.prp.2022.153909. Online ahead of print.ABSTRACTClear cell squamous cell carcinoma (CCSCC), where cells show abundant clear cytoplasm, -is a variant of squamous cell carcinoma (SCC) and a rare entity in the oral cavity. The characteristics of CCSCC, especially in immunohistochemical features, remain unclear. We characterized a case of CCSCC arising from the oral mucosal epithelium of tongue, where the clear cell lesion accounted for a predominant portion of the tumor. This CCSCC, which was partially surrounded by conventional SCC, exhibited cellular atypia immunohisto...
Source: Pathology, Research and Practice - May 13, 2022 Category: Pathology Authors: Kana Hasegawa Shinsuke Fujii Kari J Kurppa Takashi Maehara Kazunari Oobu Seiji Nakamura Tamotsu Kiyoshima Source Type: research
Identification and function of circular RNA hsa_circ_0071106: A novel biomarker for differentiation degree of esophageal squamous cell carcinoma
CONCLUSION: Hsa_circ_0071106 was a downregulated circRNA related to ESCC and promoted ESCC progression by regulating cell migration and invasion. These findings suggest that hsa_circ_0071106 could be a promising diagnostic biomarker and potential therapeutic target and might predict ESCC's histological differentiation degree.PMID:35390631 | DOI:10.1016/j.prp.2022.153875
Source: Pathology, Research and Practice - April 7, 2022 Category: Pathology Authors: Ying Tang Zhikui Gao Ran Liu Source Type: research
Myocyte-specific enhancer factor 2D promotes tumorigenesis and progression in tongue squamous cell carcinoma.
Abstract
Tongue squamous cell carcinoma (TSCC) ranks as one of the most common cancers worldwide and has a poor prognosis. Myocyte-specific enhancer factor 2 (MEF2D) has recently been considered as a novel factor involved in cancer development. In the present study, the function and underlying mechanism of MEF2D in TSCC were investigated. The levels of MEF2D mRNA and protein were determined in human TSCC samples by RT-qPCR and western blot, respectively. The interaction between MEF2D expression and clinicopathologic features was evaluated by IHC and analysis of clinical information. MEF2D-mediated effects on proli...
Source: International Journal of Clinical and Experimental Pathology - June 9, 2020 Category: Pathology Authors: Liu Y, Yu W, Zhu Y, Sun Z, Huang X, Zhou J, Yao R, Zhang Q, Qiu J, Yue L Tags: Int J Clin Exp Pathol Source Type: research
LRRC8A Expression Influences Growth of Esophageal Squamous Cell Carcinoma
The volume-regulated anion channel is composed of leucine-rich repeat –containing protein A (LRRC8A), and is activated by hypotonic conditions to implement the process of regulatory volume decrease. The role of LRRC8A in regulating genes related to progression of esophageal squamous cell carcinoma (ESCC) was investigated, as well as the prognostic significance of LR RC8A expression in this tumor. Knockdown experiments were conducted using ESCC cell lines and LRRC8A siRNA to assess the influence of this protein on tumor function.
Source: American Journal of Pathology - July 15, 2019 Category: Pathology Authors: Tomoki Konishi, Atsushi Shiozaki, Toshiyuki Kosuga, Michihiro Kudou, Katsutoshi Shoda, Tomohiro Arita, Hirotaka Konishi, Shuhei Komatsu, Takeshi Kubota, Hitoshi Fujiwara, Kazuma Okamoto, Mitsuo Kishimoto, Eiichi Konishi, Yoshinori Marunaka, Eigo Otsuji Tags: Regular Article Source Type: research
LRRC8A Expression Influences Growth of Esophageal Squamous Cell Carcinoma.
Abstract
The volume-regulated anion channel is composed of leucine-rich repeat-containing protein A (LRRC8A), and is activated by hypotonic conditions to implement the process of regulatory volume decrease. The role of LRRC8A in regulating genes related to progression of esophageal squamous cell carcinoma (ESCC) was investigated, as well as the prognostic significance of LRRC8A expression in this tumor. Knockdown experiments were conducted using ESCC cell lines and LRRC8A siRNA to assess the influence of this protein on tumor function. In addition, the gene expression profile of ESCC was determined by microarray a...
Source: The American Journal of Pathology - July 15, 2019 Category: Pathology Authors: Konishi T, Shiozaki A, Kosuga T, Kudou M, Shoda K, Arita T, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Konishi E, Marunaka Y, Otsuji E Tags: Am J Pathol Source Type: research
Astroprincin (FAM171A1, C10orf38): A regulator of human cell shape and invasive growth.
Abstract
Our group originally found and cloned cDNA for a 98 kDa type 1 transmembrane glycoprotein of unknown function. Due to its abundant expression in astrocytes it was called the protein astroprincin (APCN). Two thirds of the evolutionarily conserved protein is intracytoplasmic whereas the extracellular domain carries two N-glycosidic side chains. APCN is physiologically expressed in placental trophoblasts, skeletal and hearth muscle, and kidney and pancreas. Overexpression of APCN (cDNA) in various cell lines induced sprouting of slender projections whereas knockdown of APCN expression by siRNA caused disappe...
Source: The American Journal of Pathology - October 9, 2018 Category: Pathology Authors: Rasila T, Saavalainen O, Attalla H, Lankila P, Haglund C, Hölttä E, Andersson LC Tags: Am J Pathol Source Type: research
Macrophage migration inhibitory factor is a novel prognostic marker for human oral squamous cell carcinoma
The objective of this study was to investigate the expression of MIF and its receptor CD74 in OSCC tissues, and to study the function of MIF in OSCC cells. Tissues of 90 patients with OSCC from the School of Stomatology, China Medical University were collected, and immunohistochemical staining and quantitative reverse transcription polymerase chain reaction were performed for MIF and CD74. The possible correlations between MIF and CD74 and clinical characteristics were analysed. The Kaplan-Meier analysis was used to determine the survival rates of patients. In addition, the proliferation and invasion of OSCC cells were eva...
Source: Pathology Research and Practice - July 10, 2018 Category: Pathology Source Type: research
Loss of CYLD promotes cell invasion via ALK5 stabilization in oral squamous cell carcinoma
This study aimed to clarify the roles of CYLD in OSCC progression. Our immunohistochemical analyses revealed significantly reduced CYLD expression in invasive areas in OSCC tissues, whereas CYLD expression was conserved in normal epithelium and carcinoma in situ. Furthermore, downregulation of CYLD by siRNA led to acquisition of mesenchymal features and increased migratory and invasive properties in OSCC cells and HaCaT keratinocytes. It is interesting that CYLD knockdown promoted transforming growth factor‐β (TGF‐β) signalling by inducing stabilization of TGF‐β receptor I (ALK5) in a cell autonomous fashion. In a...
Source: The Journal of Pathology - December 1, 2017 Category: Pathology Authors: Satoru Shinriki, Hirofumi Jono, Manabu Maeshiro, Takuya Nakamura, Jianying Guo, Jian ‐Dong Li, Mitsuharu Ueda, Ryoji Yoshida, Masanori Shinohara, Hideki Nakayama, Hirotaka Matsui, Yukio Ando Tags: Original Paper Source Type: research
Effects of microRNA-708 on Epithelial-Mesenchymal Transition, Cell Proliferation and Apoptosis in Melanoma Cells by Targeting LEF1 through the Wnt Signaling Pathway.
This study was conducted in order to elucidate the role microRNA-708 (miR-708) plays between proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) involving melanoma cells by targeting using LEF1 through the Wnt signaling pathway. Male Kunming mice were selected and subsequently divided into normal and model groups to take part in this study. Following cell line selection, the B16 cells with the highest miR-708 expression were selected and assigned into the control, blank, negative control (NC), miR-708 mimic, miR-708 inhibitor, siRNA-LEF1, and miR-708 inhibitor + siRNA-LEF1 groups. A Bioinformati...
Source: Pathology Oncology Research - November 14, 2017 Category: Pathology Authors: Song XF, Wang QH, Huo R Tags: Pathol Oncol Res Source Type: research
