Astroprincin (FAM171A1, C10orf38): A regulator of human cell shape and invasive growth.

Astroprincin (FAM171A1, C10orf38): A regulator of human cell shape and invasive growth. Am J Pathol. 2018 Oct 09;: Authors: Rasila T, Saavalainen O, Attalla H, Lankila P, Haglund C, Hölttä E, Andersson LC Abstract Our group originally found and cloned cDNA for a 98 kDa type 1 transmembrane glycoprotein of unknown function. Due to its abundant expression in astrocytes it was called the protein astroprincin (APCN). Two thirds of the evolutionarily conserved protein is intracytoplasmic whereas the extracellular domain carries two N-glycosidic side chains. APCN is physiologically expressed in placental trophoblasts, skeletal and hearth muscle, and kidney and pancreas. Overexpression of APCN (cDNA) in various cell lines induced sprouting of slender projections whereas knockdown of APCN expression by siRNA caused disappearance of actin stress fibres. Immunohistochemical staining of human cancers for endogenous APCN showed elevated expression in invasive tumor cells compared to intratumoral cells. Human melanoma cells (SK-MEL-28) transfected with APCN cDNA acquired ability of invasive growth in semisolid medium (Matrigel) not seen with control cells. A conserved carboxyterminal stretch of 21 amino acids was found to be essential for APCN to induce cell sprouting and invasive growth. Yeast-2-hybrid screening revealed several interactive partners of which ornithine decarboxylase (ODC) antizyme-1, NEEP21 (NSG1), and ADAM10 were validated by ...
Source: The American Journal of Pathology - Category: Pathology Authors: Tags: Am J Pathol Source Type: research