Grade I, II and III Follicular Lymphomas Express Ig VH Genes with Different Patterns of Somatic Mutation.
Z, Matolcsy A Abstract Follicular lymphoma (FL) is an indolent, B-cell, non-Hodgkin's lymphoma with varying cytological appearance and clinical behavior. The genetic hallmark of FL is the t(14;18) translocation, and as a germinal center derived entity it is also characterized by somatic hypermutation of the immunoglobulin heavy chain (IgH) gene. In an attempt to correlate this molecular signature with the cytological grading of FL, we have analyzed the IgH variable (IgVH), regions in all cytological grades of FL. Four FL cases showing t(14;18) translocation were classified into grade I-III categories accordin...
Source: Pathology Oncology Research - July 23, 2020 Category: Pathology Authors: Csernus B, Timár B, Fülöp Z, Matolcsy A Tags: Pathol Oncol Res Source Type: research

The Proportion of Tumour-Stroma in Metastatic Lymph Nodes is An Accurately Prognostic Indicator of Poor Survival for Advanced-Stage Colon Cancers.
This study aimed to determine the prognostic effect of the proportion of tumour-stroma (PTS) in metastatic lymph nodes of advanced-stage colon cancers (CCs). We investigated PTS in positive lymph nodes of stage III-IV CC patients who underwent surgical treatment between 2004 and 2014. We used a standard approach in methodology. PTS was significantly associated with prognostic factors in the metastatic lymph nodes (perineural invasion [p = 0.031], lymphatic invasion [p = 0.032], invasive margin [p = 0.043], advanced pT [p = 0.020], and margin involvement [p =&th...
Source: Pathology Oncology Research - July 21, 2020 Category: Pathology Authors: Zengin M, Benek S Tags: Pathol Oncol Res Source Type: research

Examination of Tumor Regression Grading Systems in Breast Cancer Patients Who Received Neoadjuvant Therapy.
erni G, Zombori T Abstract Neoadjuvant therapy is a common form of treatment in locally advanced breast cancer (LABC) patients. Besides some guidelines for grading regression, a standardized general scheme is not yet available. The aim of our study was to compare the prognostic impact of different regression grading systems, namely the TR/NR, Chevallier, Sataloff, Denkert-Sinn, Miller-Payne, NSABP-B18, Residual Disease in Breast and Nodes and Residual Cancer Burden (RCB) on disease-free (DFS) and overall survival (OS). Data of 746 breast cancer patients treated in neoadjuvant setting between 1999 and 2019 have bee...
Source: Pathology Oncology Research - July 20, 2020 Category: Pathology Authors: Sejben A, Kószó R, Kahán Z, Cserni G, Zombori T Tags: Pathol Oncol Res Source Type: research

Chromosome X riddle in SARS-CoV-2 (COVID-19) - related lung pathology.
PMID: 32691391 [PubMed - as supplied by publisher] (Source: Pathology Oncology Research)
Source: Pathology Oncology Research - July 20, 2020 Category: Pathology Authors: Tsiambas E, Chrysovergis A, Papanikolaou V, Mastronikolis N, Ragos V, Kavantzas N, Lazaris AC, Patsouris E, Riziotis C, Paschopoulos M, Kyrodimos E Tags: Pathol Oncol Res Source Type: research

Prognostic Role of Immune Markers in Triple Negative Breast Carcinoma.
In conclusion, we suggest that composition, density and localization of the immune cells and the check point molecules are important prognostic parameters in TNBC. Immunohistochemistry can be used as an accessible and less expensive tool to demonstrate TIME. PMID: 32681436 [PubMed - as supplied by publisher] (Source: Pathology Oncology Research)
Source: Pathology Oncology Research - July 17, 2020 Category: Pathology Authors: Sahin Ozkan H, Ugurlu MU, Yumuk PF, Kaya H Tags: Pathol Oncol Res Source Type: research

Expression of SIRT1, SIRT3 and SIRT6 Genes for Predicting Survival in Triple-Negative and Hormone Receptor-Positive Subtypes of Breast Cancer.
Abstract Triple-negative breast cancer (TNBC) is characterized by aggressive phenotype and a poorer prognosis compared to the estrogen and progesterone receptor positive, Her2 negative (ER + PR + Her2-) breast cancer. Increasing evidence suggests that sirtuins, a family of histone deacetylases, could have an important role in aggressiveness of TNBC's. The current study evaluated the potential clinical relevance of SIRT1, SIRT3 and SIRT6 gene expressions in two prognostically distinctive subtypes of breast cancer, the most aggressive TNBC and the least aggressive ER + PR&th...
Source: Pathology Oncology Research - July 17, 2020 Category: Pathology Authors: Uzelac B, Krivokuca A, Brankovic-Magic M, Magic Z, Susnjar S, Milovanovic Z, Supic G Tags: Pathol Oncol Res Source Type: research

Secreted Frizzled-Related Protein 4 (SFRP4) Is an Independent Prognostic Marker in Prostate Cancers Lacking TMPRSS2: ERG Fusions.
mayer D, Izbicki JR, Simon R, Sauter G, Minner S, Steurer S, Meiners J Abstract Secreted frizzled-related protein 4 (SFRP4) controls WNT signaling and is thought to play a role for tumor aggressiveness. Here, we analyzed a tissue microarray containing 11,152 prostate cancers with pathological, clinical and molecular data by immunohistochemistry. SFRP4 expression was higher in cancer than in non-neoplastic acinar cells. SFRP4 staining was seen in 64.9% of tumors and classified as weak in 33.2%, moderate in 23.9% and strong in 7.8% of cancers. SFRP4 overexpression was linked to advanced tumor stage, high classical/q...
Source: Pathology Oncology Research - July 16, 2020 Category: Pathology Authors: Bernreuther C, Daghigh F, Möller K, Hube-Magg C, Lennartz M, Lutz F, Rico SD, Fraune C, Dum D, Luebke AM, Eichenauer T, Möller-Koop C, Schlomm T, Wittmer C, Huland H, Heinzer H, Graefen M, Haese A, Burandt E, Tsourlakis MC, Clauditz TS, Höflmayer D, Iz Tags: Pathol Oncol Res Source Type: research

Papillary Endothelial Hyperplasia (Masson Tumor) of the Hand. Surgical and Pathological Consideration from Seven Cases Using New Vascular Markers.
We present seven cases of Masson tumor of the hand; wanting to reveal its nature using new vascular markers and discuss the treatment options and expected outcomes, present clinical and radiological features that may aid diagnosis and also offer treatment plans. A multicenter retrospective study was performed between January 2014 and November 2019. Immunohistochemical stains of Glut1, WT1, ERG, CD31 and alpha smooth muscle actin (ASMA) were performed on each cases. We found seven cases during the examined period. 4 out of 7 cases were women. All lesions occurred in the hands. 3 out of 7 cases appeared in a previously prese...
Source: Pathology Oncology Research - July 15, 2020 Category: Pathology Authors: Patai BB, Peterfy N, Szakacs N, Sapi Z, Hetthessy JR Tags: Pathol Oncol Res Source Type: research

The Role of Time as a Prognostic Factor in Pediatric Brain Tumors: a Multivariate Survival Analysis.
Juárez-Villegas L, Murata C Abstract There is no evidence that prolonged pre diagnostic symptomatic intervals (PSI) increases the risk of death in pediatric brain tumors. When investigating the role of time previous research had not controlled for confounding variables or measured the pretreatment interval (PTI). We use the term global delay interval (GDI) to describe the sum of PSI and PTI. The aim of this research was to evaluate whether there was a decrease in the probability of survival in children with brain tumors due to a prolonged PSI, PTI and GDI, using a multivariate survival analysis. We retrosp...
Source: Pathology Oncology Research - July 13, 2020 Category: Pathology Authors: Barragán-Pérez EJ, Altamirano-Vergara CE, Alvarez-Amado DE, García-Beristain JC, Chico-Ponce-de-León F, González-Carranza V, Juárez-Villegas L, Murata C Tags: Pathol Oncol Res Source Type: research

Metabolic Parameters as Predictors for Progression Free and Overall Survival of Patients with Metastatic Colorectal Cancer.
Abstract We tested the prognostic relevance of metabolic parameters and their relative changes in patients with metastatic colorectal cancer (mCRC) treated with monoclonal antibody and chemotherapy. SUVmax (standardized uptake volume), SAM (standardized added metabolic activity) and TLG (total lesion glycolysis) are assessed with 18F-fluorodeoxyglucosepositron emission tomography and computed tomography (FDG-PET/CT) to evaluate total metabolic activity of malignant processes. Our purpose was to investigate the change of glucose metabolism in relation to PFS (progression free survival) and OS (overall survival). Fi...
Source: Pathology Oncology Research - July 13, 2020 Category: Pathology Authors: Nemeth Z, Wijker W, Lengyel Z, Hitre E, Borbely K Tags: Pathol Oncol Res Source Type: research

The Clinical Impact of Re-biopsies in Lung Adenocarcinoma: a Retrospective Multicenter Study.
Ü Abstract If a patient's cancer progresses while undergoing targeted therapy, a re-biopsy is not mandatory. But when evaluating the benefits and risks on a case-by-case basis (transformation to small cell, assessing for a clinical trial), physicians should inform patients about the possible need for a re-biopsy (5). This was a retrospective and multicentre study. A total of 644 patients with lung adenocarcinoma were reviewed, 625 of whom were ruled eligible. From them, 399 were found to show disease progression, and 126 re-biopsies were performed. Progression status, re-biopsy sites, success of obtaining ad...
Source: Pathology Oncology Research - July 13, 2020 Category: Pathology Authors: Kabalak PA, Kızılgöz D, Kavurgacı S, Demirci NY, Yılmaz Ş, Ak G, Metintaş S, Metintaş M, Demirağ F, Yılmaz Ü Tags: Pathol Oncol Res Source Type: research

The Effect of Methylselenocysteine and Sodium Selenite Treatment on microRNA Expression in Liver Cancer Cell Lines.
In conclusion, our results revealed moderately altered miRNA expression in the cell lines (less alterations following MSC treatment), being miR-122, -199a the most affected and miR-125b, -194 the least altered miRNAs upon selenium treatment. PMID: 32656599 [PubMed - as supplied by publisher] (Source: Pathology Oncology Research)
Source: Pathology Oncology Research - July 12, 2020 Category: Pathology Authors: Lendvai G, Szekerczés T, Kontsek E, Selvam A, Szakos A, Schaff Z, Björnstedt M, Kiss A Tags: Pathol Oncol Res Source Type: research

Overexpression of Long Non-Coding RNA FGF14-AS2 Inhibits Colorectal Cancer Proliferation Via the RERG/Ras/ERK Signaling by Sponging microRNA-1288-3p.
This study aimed to investigate the role of FGF14-AS2 in colorectal cancer development. FGF14-AS2 was found as a significantly downregulated lncRNA in TCGA dataset. Via RT-qPCR, we confirmed the downregulation of FGF14-AS2 in collected colorectal carcinoma samples. Transfection of plasmid containing full length of FGF14-AS2 repressed cell proliferation and induced elevation of cell apoptosis in colorectal cancer cells. In addition, FGF14-AS2 overexpression inactivated MAPK/ERK signaling in cells. Bioinformatic analysis and subsequent cell-based assays showed that FGF14-AS2 sponging miR-1288-3p, an oncogenic miRNA in colore...
Source: Pathology Oncology Research - July 11, 2020 Category: Pathology Authors: Hou R, Liu Y, Su Y, Shu Z Tags: Pathol Oncol Res Source Type: research

Prognostic Role of Aryl Hydrocarbon Receptor Interacting Protein (AIP) Immunohistochemical Expression in Patients with Resected Gastric Carcinomas.
a Gómez de Las Heras S, Fernández Aceñero MJ Abstract Aryl hydrocarbon receptor (AHR) interacting protein (AIP) is a chaperone which binds to inactive AHR in the cell cytoplasm. AHR is best known for mediating the toxicity of halogenated aromatics, but it has also been linked to carcinogenesis and tumor progression in several tumor types. Our aims are to assess the features of AIP immunohistochemical (IHC) staining and to evaluate its possible role as a prognostic marker in gastric cancer (GC). Retrospective study of 147 cases of resected GC. Clinicopathological features were collected, tissue...
Source: Pathology Oncology Research - July 9, 2020 Category: Pathology Authors: Díaz Del Arco C, Estrada Muñoz L, Barderas Manchado R, Peláez García A, Ortega Medina L, Molina Roldán E, Solís Fernández G, García Gómez de Las Heras S, Fernández Aceñero MJ Tags: Pathol Oncol Res Source Type: research

Low Fraction Size Re-irradiation for Large Volume Recurrence of Glial Tumours.
eisz Z, Barzó P, Oláh J, Hideghéty K Abstract The aim of the present study was to evaluate the efficacy of re-irradiation (re-RT) in patients with advanced local relapses of glial tumours and to define the factors influencing the result of the hyper-fractionated external beam therapy on progression after primary management. We have analysed the data of 55 patients with brain tumours (GBM: 28) on progression, who were re-irradiated between January 2007 and December 2018. The mean volume of the recurrent tumour was 118 cm3, and the mean planning target volume (PTV) was 316 cm3, to...
Source: Pathology Oncology Research - July 9, 2020 Category: Pathology Authors: Dobi Á, Darázs B, Fodor E, Cserháti A, Együd Z, Maráz A, László S, Dodd L, Reisz Z, Barzó P, Oláh J, Hideghéty K Tags: Pathol Oncol Res Source Type: research

Increased miR-20b Level in High Grade Cervical Intraepithelial Neoplasia.
endvai G, Sobel G Abstract Cervical cancer is a common malignant tumor worldwide ranking fourth in incidence and mortality among females, which was reduced significantly by cytology screening and human papilloma virus (HPV) DNA testing. The specificity of cytology is high; however, the sensitivity is low, in contrast to the HPV DNA testing. Despite the success of these measures, new biomarkers are still considered to aim increasing sensitivity and specificity of screening and diagnosis. Significant alterations in microRNA (miRNA) expression have been detected in several cancers with variable consistency. To invest...
Source: Pathology Oncology Research - July 8, 2020 Category: Pathology Authors: Szekerczés T, Galamb Á, Varga N, Benczik M, Kocsis A, Schlachter K, Kiss A, Ács N, Schaff Z, Jeney C, Lendvai G, Sobel G Tags: Pathol Oncol Res Source Type: research

Concordance Between Biopsy and Radical Prostatectomy Gleason Scores: Evaluation of Determinants in a Large-Scale Study of Patients Undergoing RARP in Belgium.
istry Abstract To determine whether Gleason scores were concordant between prostate biopsies (bGS) and the definitive resection specimen (pGS) excised with robot-assisted radical prostatectomy (RARP); to identify clinical and pathological factors that might predict upgrading; and to evaluate how upgrading affected outcome. Between 2009 and 2016, 25 Belgian centers participated in collecting prospective data for patients that underwent RARP. We analyzed the concordance rate between the bGS and the pGS in 8021 patients with kappa statistics, and we compared concordance rates from different centers. We assessed the e...
Source: Pathology Oncology Research - July 6, 2020 Category: Pathology Authors: Soenens C, Dekuyper P, De Coster G, Van Damme N, Van Eycken E, Quackels T, Roumeguère T, Van Cleynenbreugel B, Joniau S, Ameye F, Be-RALP registry Tags: Pathol Oncol Res Source Type: research

Novel CXCR4 Inhibitor CPZ1344 Inhibits the Proliferation, Migration and Angiogenesis of Glioblastoma.
Abstract Glioblastoma (GBM) are life-threatening tumors with a poor prognosis and low cure rates. GBMs are malignant brain tumors that develop from astrocytes. Most GBMs are not inherited and occur sporadically. GBM recurrence after standard treatment has led to the assessment of agents targeting the CXCR4 chemokine receptor as alternative drug target for much needed GBM therapeutics. In present study, a novel CXCR4 inhibitor modified with a picolinamide scaffold (CPZ1344) was designed and synthesized. Its anti-GBM function was then evaluated. Our results showed that CPZ1344 reduced the growth of GBM cells in a co...
Source: Pathology Oncology Research - July 6, 2020 Category: Pathology Authors: Luo Z, Wang B, Chen Y, Liu H, Shi L Tags: Pathol Oncol Res Source Type: research

A Clinicopathological Approach to Odontogenic Cysts: the Role of Cytokeratin 17 and bcl2 Immunohistochemistry in Identifying Odontogenic Keratocysts.
cute;th Z, Cserni G Abstract Odontogenic keratocysts (OKCs) are developmental cysts of the jaws that require proper diagnosis due to their potential for local aggressive growth and recurrences. OKCs have a typical parakeratotic epithelium demonstrating transepithelial cytokeratin 17 (CK17) and basal bcl2 staining on immunohistochemistry (IHC), which distinguishes them from other common jaw cysts. Secondary to inflammation, the epithelial lining may be altered and loses the typical IHC phenotype. The aim of the present study was to analyse a series of consecutive jaw cysts for their expression of CK17 and bcl2 and ...
Source: Pathology Oncology Research - July 6, 2020 Category: Pathology Authors: Cserni D, Zombori T, Vörös A, Stájer A, Rimovszki A, Daru K, Baráth Z, Cserni G Tags: Pathol Oncol Res Source Type: research

The Ratio of ssDNA to dsDNA in Circulating Cell-Free DNA Extract is a Stable Indicator for Diagnosis of Gastric Cancer.
Abstract Due to the different mechanisms of cell-free DNA production, the single-stranded DNA to double-stranded DNA ratio in blood maybe different between healthy individuals and gastric cancer (GC) patients. We aimed to explore the potential application of this ratio in GC diagnosis. The plasma cell-free DNA extracts from 118 healthy individuals and 106 GC patients were prepared. The levels of single-stranded DNA or double-stranded DNA in plasma, and the single-stranded DNA to double-stranded DNA ratio on the diagnostic efficiency for GC were assessed with ROC curve. The relationships between this ratio and the ...
Source: Pathology Oncology Research - July 6, 2020 Category: Pathology Authors: Huang X, Zhao Q, An X, Pan J, Zhao L, Shen L, Xu Y, Yuan D Tags: Pathol Oncol Res Source Type: research

Clinical Significance of Trk Receptor Expression as a New Therapeutic Target in Hepatocellular Carcinoma.
Abstract Oncogenic fusion of the tropomyosin receptor kinase (Trk) receptor family encoded by the NTRK gene has been found in several carcinomas. About ten targeted therapies have been developed and clinical trials are in progress. However, the results of studies on expression of the Trk receptor in HCC have not yet been published. Immunohistochemical staining was performed using anti-TrkA+B+C antibody (ab181560, Abcam) in 288 curatively resected primary HCC samples, and the correlation between Trk expression and NTRK copy number was assessed. Targeted next generation sequencing was performed in cases with Trk ove...
Source: Pathology Oncology Research - July 4, 2020 Category: Pathology Authors: Choi S, Park S, Cho YA, Park CK, Ha SY Tags: Pathol Oncol Res Source Type: research

Evaluation of Next Generation Sequencing for Detecting HER2 Copy Number in Breast and Gastric Cancers.
Abstract Amplicon-based next generation sequencing (NGS) approaches have been preferentially adopted by the clinical laboratories on the basis of a short turnaround time (TAT) and small DNA input needs. However, little work has been done to assess the amplicon-based NGS methods for copy number variation (CNV) detection in comparison with current standard methods like immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). The correlation between NGS based CNV detection and the later standard methods has remained unexplored. We developed an amplicon-based panel to detect human epidermal receptor g...
Source: Pathology Oncology Research - July 3, 2020 Category: Pathology Authors: Niu D, Li L, Yu Y, Zang W, Li Z, Zhou L, Jia L, Rao G, Gao L, Cheng G, Ji K, Lin D Tags: Pathol Oncol Res Source Type: research

Prognostic Significance of Lacunarity in Preoperative Biopsy of Colorectal Cancer.
ć A, Konjevoda P Abstract The quantity and quality of preoperative material in colorectal cancer is often limiting factor in determination of risk factors and therapy planning. The most important negative prognostic factors are intravascular and perineural invasion, as well as tumor budding. Usually, the only parameter available in preoperative biopsy is tumor budding. However, the growing body of evidence suggests that cancer differentiation based on the poorly differentiated clusters has better prognostic value. The limiting factor in applying of these new parameters is reproducible, simple, cheap and fast meth...
Source: Pathology Oncology Research - July 2, 2020 Category: Pathology Authors: Aralica G, Šarec Ivelj M, Pačić A, Baković J, Milković Periša M, Krištić A, Konjevoda P Tags: Pathol Oncol Res Source Type: research

Gene Expression Signature of BRAF Inhibitor Resistant Melanoma Spheroids.
te;ny R, Balazs M Abstract In vitro cell cultures are frequently used to define the molecular background of drug resistance. The majority of currently available data have been obtained from 2D in vitro cultures, however, 3D cell culture systems (spheroids) are more likely to behave similarly to in vivo conditions. Our major aim was to compare the gene expression signature of 2D and 3D cultured BRAFV600E mutant melanoma cell lines. We successfully developed BRAF-drug resistant cell lines from paired primary/metastatic melanoma cell lines in both 2D and 3D in vitro cultures. Using Affymetrix Human Gene 1.0 ST arrays...
Source: Pathology Oncology Research - July 1, 2020 Category: Pathology Authors: Koroknai V, Patel V, Szász I, Ádány R, Balazs M Tags: Pathol Oncol Res Source Type: research

Landscape of RAS Variations in 17,993 Pan-cancer Patients Identified by Next-generation Sequencing.
Abstract RAS family genes (HRAS, KRAS and NRAS) were frequently observed in several tumors. The expression of constitutively active RAS proteins mediated by RAS variations promote the development of tumors. KRAS is an important prognostic and drug resistance biomarker. It would also be a promising drug target. Several trials which evaluating the efficacy of RAS G12C inhibitor in solid tumors are initiated. Herein, we analyzed the alterations status of KRAS/NRAS/HRAS across diverse solid tumors. The sing nucleotide variants (SNV) and copy number variants (CNV) data of 17993 Chinese patients from 22 types of cancer ...
Source: Pathology Oncology Research - June 30, 2020 Category: Pathology Authors: Zhou S, Zhang D, Li J, Zhao J, Wang G, Zhang Y, Bai Y, Chen D, Wu H Tags: Pathol Oncol Res Source Type: research

The Role of Immunohistochemical Overexpression of p53 as Adverse Prognostic Factor in Primary Testicular Diffuse Large B Cell Lymphoma.
PMID: 32602002 [PubMed - as supplied by publisher] (Source: Pathology Oncology Research)
Source: Pathology Oncology Research - June 29, 2020 Category: Pathology Authors: Hatzl S, Posch F, Schulz E, Gornicec M, Deutsch A, Beham-Schmid C, Pichler M, Greinix H, Sill H, Zebisch A, Neumeister P, Prochazka KT Tags: Pathol Oncol Res Source Type: research

KRAS Status is Associated with Metabolic Parameters in Metastatic Colorectal Cancer According to Primary Tumour Location.
Abstract Colorectal cancer (CRC) is characterized by complex interplay between macroenvironmental factors and tumour microenvironment, leading to variable outcomes in CRC patients. To date, there is still a need to identify macroenvironment/microenvironment factors that could define subgroup of patients that would benefit from specific anti-cancer treatment in order to improve patient selection for individualized targeted-based therapy. Aim of this study was to evaluate associations between metabolic parameters and KRAS status in metastatic CRC (mCRC) according to a new tumour site classification. Retrospective da...
Source: Pathology Oncology Research - June 27, 2020 Category: Pathology Authors: Tabuso M, Christian M, Kimani PK, Gopalakrishnan K, Arasaradnam RP Tags: Pathol Oncol Res Source Type: research

The Prognostic Value of PARP Expression in High-Grade Epithelial Ovarian Cancer.
G, Póka R Abstract In an attempt to clarify the prognostic relevance of poly (ADP-ribose) polymerase (PARP) expression, we analysed the clinical data of 86 high-grade epithelial ovarian cancer (EOC) cases in which PARP immunohistochemistry results were available. Immunostaining to highlight PARP protein expression was performed using a Leica Bond MAX Immunostainer (Leica Microsystems, Wetzlar, Germany). We applied a rabbit polyclonal anti-PARP antibody (ab6079 330, Abcam, Cambridge, UK) for the specific reaction. The intensity and distribution of immunostaining were assessed by light microscopy (Leica DM25...
Source: Pathology Oncology Research - June 27, 2020 Category: Pathology Authors: Molnár S, Beke L, Méhes G, Póka R Tags: Pathol Oncol Res Source Type: research

HDAC Inhibition Induces PD-L1 Expression in a Novel Anaplastic Thyroid Cancer Cell Line.
C, Hegedűs B Abstract While papillary thyroid cancer (PTC) has largely favorable prognosis, anaplastic thyroid cancer (ATC) is a rare but extremely aggressive malignancy with grim clinical outcome. Even though new therapeutic options are emerging for ATC, additional preclinical models and novel combinations are needed for specific subsets of patients. We established a novel cell line (PF49) from the malignant pleural effusion of a 68-year-old male patient with ATC that rapidly transformed from a BRAF and TERT promoter mutant PTC. PF49 cells demonstrated a robust migratory activity in vitro and strong invasive cap...
Source: Pathology Oncology Research - June 26, 2020 Category: Pathology Authors: Hegedűs L, Rittler D, Garay T, Stockhammer P, Kovács I, Döme B, Theurer S, Hager T, Herold T, Kalbourtzis S, Bankfalvi A, Schmid KW, Führer D, Aigner C, Hegedűs B Tags: Pathol Oncol Res Source Type: research

Prognostic Value of Systemic Inflammatory Biomarkers in Patients with Metastatic Renal Cell Carcinoma.
Abstract Metastatic renal cell carcinoma (mRCC) encompasses a heterogeneous group of neoplasms with distinct clinical behavior and prognoses. As a result of the increasing number of therapeutic options in the metastatic setting, it is crucial to improve prognostic stratification ability. We aimed to evaluate the prognostic value of neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and combination platelet count and neutrophil lymphocyte ratio (COP-NLR) in patients with mRCC. We evaluated a cohort of mRCC patients treated with first-line pazopanib or sunitinib. Levels of NLR, PLR and COP-NLR ...
Source: Pathology Oncology Research - June 24, 2020 Category: Pathology Authors: Nader Marta G, Isaacsson Velho P, Bonadio RRC, Nardo M, Faraj SF, de Azevedo Souza MCL, Muniz DQB, Bastos DA, Dzik C Tags: Pathol Oncol Res Source Type: research

Expression of the Serrated Markers Annexin A10 or Gremlin1 in Colonic Adenocarcinomas: Morphology and Prognostic Values.
mbi C Abstract Describe clinical, histological and molecular charatcteristics and prognosis values of the serrated candidate markers AnnexinA10 and Gremlin1 in colon adenocarcinomas. Immunohistochemical expression of AnnexinA10 and Gremlin1 was evaluated on 346 colonic adenocarcinomas. Clinicopathological, molecular features and prognostic characteristics were then evaluated. A total of 40 colonic adenocarcinomas expressed AnnexinA10 (11.6%) and, 115 expressed Gremlin1 (40.4%). AnnexinA10 expression was significantly associated, on univariate analyses, with female gender (p = 0.03), right tumor location (p 
Source: Pathology Oncology Research - June 24, 2020 Category: Pathology Authors: Marquet B, Marchal Bressenot A, Fichel C, Bouland N, Barbe C, Bouché O, Kianmanesh R, Diebold MD, Boulagnon-Rombi C Tags: Pathol Oncol Res Source Type: research

Elevated Microparticles, Thrombin-antithrombin and VEGF Levels in Colorectal Cancer Patients Undergoing Chemotherapy.
Abstract Hypercoagulable state and neoangiogenesis are common phenomena associated with malignancy. Cancer patients have increased levels of circulating endothelium-derived microparticles (EMPs), which have been hypothesized to be involved in numerous pathophysiological processes. Hemostasis and angiogenesis are also activated in colorectal cancer (CRC) patients. The study aimed to investigate potential influence of chemotherapy on EMPs, thrombin anti-thrombin complex (TAT) and vascular endothelial growth factor (VEGF) levels in CRC patients undergoing chemotherapy. The study group consisted of 18 CRC patients: 8 ...
Source: Pathology Oncology Research - June 24, 2020 Category: Pathology Authors: Wojtukiewicz MZ, Mysliwiec M, Sierko E, Sobierska M, Kruszewska J, Lipska A, Radziwon P, Tucker SC, Honn KV Tags: Pathol Oncol Res Source Type: research

Predictive Markers of First Line Pazopanib Treatment in Kidney Cancer.
yi A, Kiss E, Biró K Abstract Real-world evidence from clinical practices is fundamental for understanding the efficacy and tolerability of medicinal products. Patients with renal cell cancer were studied to gain data not represented by analyses conducted on highly selected patients participating in clinical trials. Our goal was to retrospectively collect data from patients with advanced renal tumours treated with pazopanib (PZ) to investigate the efficacy, frequency of side effects, and searching for predictive markers. Eighty-one patients who had received PZ therapy as first-line treatment were retrospect...
Source: Pathology Oncology Research - June 22, 2020 Category: Pathology Authors: Küronya Z, Szőnyi MD, Nagyiványi K, Gyergyay F, Géczi L, Budai B, Martin T, Ladányi A, Kiss E, Biró K Tags: Pathol Oncol Res Source Type: research

De Novo and Nevus-Associated Melanomas: Different Histopathologic Characteristics but Similar Survival Rates.
In conclusion, even though de novo melanomas are more significantly correlated with aggressive histopathologic variables, such as tumor depth, ulceration and high mitotic rate, the survival rates of de novo and nevus-associated melanomas are similar. PMID: 32572820 [PubMed - as supplied by publisher] (Source: Pathology Oncology Research)
Source: Pathology Oncology Research - June 22, 2020 Category: Pathology Authors: Tas F, Erturk K Tags: Pathol Oncol Res Source Type: research

Drastic Reduction of Turnaround Time After Implementation of a Fully Automated Assay for RAS-BRAF Mutations in Colorectal Cancer: A Pilot Prospective Study in Real-life Conditions.
In conclusion, this pilot project shows that the Idylla™ platform for testing KRAS and NRAS/BRAF mutations allows an optimized turnaround time from test prescription to reception of results. PMID: 32572821 [PubMed - as supplied by publisher] (Source: Pathology Oncology Research)
Source: Pathology Oncology Research - June 22, 2020 Category: Pathology Authors: Rossat S, Perrier H, Lefevre M, Louvet C, Le Berre N, Chamois J, Dorel M, Vacque D, Guillaudeau A, Genet D, Maillet E, Triby S, Sabourin JC Tags: Pathol Oncol Res Source Type: research

Predictive Value of CD8 Expression and FoxP3 Methylation in Nasopharyngeal Carcinoma Patients Treated with Chemoradiotherapy in a Non-endemic Area.
anchin G Abstract Undifferentiated Nasopharyngeal Carcinoma (UNPC) is associated with Epstein-Barr Virus (EBV) and characterized by an abundant immune infiltrate potentially influencing the prognosis. Thus, we retrospectively assessed the significance of immunosuppression in the UNPC microenvironment as prognostic biomarker of treatment failure in a non-endemic area, and monitored the variation of systemic EBV-specific immunity before and after chemoradiotherapy (CRT). DNA and RNA were extracted from diagnostic biopsies obtained by tumor and adjacent mucosa from 63 consecutive EBV+ UNPC patients who underwent radi...
Source: Pathology Oncology Research - June 21, 2020 Category: Pathology Authors: Muraro E, Vaccher E, Furlan C, Fratta E, Fanetti G, Fae' DA, Martorelli D, Cangemi M, Polesel J, Navarria F, Gobitti C, Comaro E, Scaini C, Pratesi C, Zanussi S, Lupato V, Grando G, Giacomarra V, Sulfaro S, Barzan L, Dolcetti R, Steffan A, Canzonieri V, F Tags: Pathol Oncol Res Source Type: research

Label-Free Semiquantitative Liquid Chromatography-Tandem Mass Spectrometry Proteomics Analysis of Laryngeal/Hypopharyngeal Squamous Cell Carcinoma on Formalin-Fixed, Paraffin-Embedded Tissue Samples - a Pilot Study.
Abstract Squamous cell carcinoma (SCC) of the head and neck region is the sixth most frequent malignancy with high mortality rate. Due to its poor prognosis it is considered a growing public health problem worldwide inspite of existing treatment modalities. Thus, early diagnosis of new diseases and recurrences is emerging on one hand, but on the other hand troublesome in the lack of reliable tumor markers in this field. The rapid development of proteomics has opened new perspectives in tumor marker discovery. Liquid chromatography/mass spectrometry (LC/MS) as the gold standard in proteomics enables the semi-quanti...
Source: Pathology Oncology Research - June 21, 2020 Category: Pathology Authors: Burian A, Lujber L, Gerlinger I, Jarai T, Orosz E, Turiak L, Acs A, Hegedus Z, Peter AK, Tornoczki T, Gombos K, Mark L Tags: Pathol Oncol Res Source Type: research

Architectural Grade Combined With Spread Through Air Spaces (STAS) Predicts Recurrence and is Suitable for Stratifying Patients Who Might Be Eligible for Lung Sparing Surgery for Stage I Adenocarcinomas.
Furák J Abstract The spread through air spaces (STAS) has a main role in local recurrence of stage I lung adenocarcinomas (LAs), therefore its presence might question sublobar resection as a therapeutic option. The aim of our study was to evaluate the distribution of STAS in stage I LAs, to stratify patients according to local recurrence and to identify a group of patients who might be suitable for sublobar surgery. Patients resected with LA were included. The presence of STAS was recorded on hematoxylin eosin stained slides and clinicopathological data were obtained from medical charts. Overall survival (...
Source: Pathology Oncology Research - June 20, 2020 Category: Pathology Authors: Zombori T, Sejben A, Tiszlavicz L, Cserni G, Pálföldi R, Csada E, Furák J Tags: Pathol Oncol Res Source Type: research

Comparison of Nottingham Prognostic Index, PREDICT and PrognosTILs in Triple Negative Breast Cancer -a Retrospective Cohort Study.
rni G Abstract Triple-negative breast cancer (TNBC) represents a heterogenous subtype of breast cancer with generally poor prognosis. The prediction of its prognosis remains essential to clinicians in their therapeutical decision-making process. The aim of our study was to compare the validity of three multivariable analysis derived prognostic systems, the Nottingham Prognostic Index (NPI), PREDICT and PrognosTILs (a prognosticator including tumor infiltrating lymphocytes, TILs) in a series of TNBCs. Patients operated on with TNBC at the Department of Surgery, Bács-Kiskun County Teaching Hospital, Kecskem&e...
Source: Pathology Oncology Research - June 20, 2020 Category: Pathology Authors: Sejben A, Nyári T, Zombori T, Cserni G Tags: Pathol Oncol Res Source Type: research

A Three-microRNA Panel in Serum: Serving as a Potential Diagnostic Biomarker for Renal Cell Carcinoma.
CONCLUSIONS: The three-miRNA panel in serum could serve as a non-invasive diagnostic biomarker of RCC. PMID: 32556891 [PubMed - as supplied by publisher] (Source: Pathology Oncology Research)
Source: Pathology Oncology Research - June 18, 2020 Category: Pathology Authors: Huang G, Li X, Chen Z, Wang J, Zhang C, Chen X, Peng X, Liu K, Zhao L, Lai Y, Ni L Tags: Pathol Oncol Res Source Type: research

In Vivo Detection of CTC and CTC Plakoglobin Status Helps Predict Prognosis in Patients with Metastatic Breast Cancer.
This study aims to detect the prognostic value of circulating tumour cell (CTC) in patients with metastatic breast cancer. In this study, 38 patients with metastatic breast cancer were enrolled. The in vivo CellCollector® method was used to detect the number of CTC in patients. Single CTC and CTC clusters were counted, and the expression of plakoglobin was also analysed. At baseline, 73.7% (28/38) of the patients were positive for ≥ 1 CTC (range, 1-14 cells). No CTC-like events were observed in the control group. Among the CTC-positive patients, 21.4% (6/28) of patients had CTC clusters, and 42.9% (12/28) of ...
Source: Pathology Oncology Research - June 18, 2020 Category: Pathology Authors: Xie N, Hu Z, Tian C, Xiao H, Liu L, Yang X, Li J, Wu H, Lu J, Gao J, Hu X, Cao M, Shui Z, Ouyang Q Tags: Pathol Oncol Res Source Type: research

Involvement of Helicobacter Pylori in Ocular Adnexa Lymphoma.
In conclusion, H. pylori seems to be involved in a subset of OAL, but the heterogeneity found needs to be investigated in further studies. The prevalence of H. pylori gastric infection in patients with OAL does not seem to differ from that of the general population. PMID: 32557170 [PubMed - as supplied by publisher] (Source: Pathology Oncology Research)
Source: Pathology Oncology Research - June 18, 2020 Category: Pathology Authors: Travaglino A, Pace M, Varricchio S, Russo D, Pugliese N, Severino A, Picardi M, Pane F, Insabato L, Staibano S, Mascolo M Tags: Pathol Oncol Res Source Type: research

Association of KLK3, VAMP8 and MDM4 Genetic Variants within microRNA Binding Sites with Prostate Cancer: Evidence from Serbian Population.
In this study, we examined a total of 1083 subjects for these three variants using Taqman® SNP Genotyping Assays. Three hundred and fifty-five samples of peripheral blood were obtained from patients with PCa and 358 samples from patients with benign prostatic hyperplasia (BPH). The control group consisted of 370 healthy volunteers. Comparisons of genotype distributions among PCa and BPH patients, as well as between PCa patients and healthy controls, yielded no evidence of association between the analyzed genetic variants and the risk of developing PCa. However, all three tested genetic variants have shown the associati...
Source: Pathology Oncology Research - June 17, 2020 Category: Pathology Authors: Kotarac N, Dobrijevic Z, Matijasevic S, Savic-Pavicevic D, Brajuskovic G Tags: Pathol Oncol Res Source Type: research

Aggressive Paraganglioma of the Urinary Bladder with Local Recurrence and Pelvic Metastasis.
We report a recurrent and metastatic paraganglioma of the urinary bladder. PMID: 32548698 [PubMed - as supplied by publisher] (Source: Pathology Oncology Research)
Source: Pathology Oncology Research - June 17, 2020 Category: Pathology Authors: Yoo KH, Choi T, Lee HL, Song MJ, Chung BI Tags: Pathol Oncol Res Source Type: research

Ruptured Aortic Aneurysm and Dissection Related Death: an Autopsy Database Analysis.
z G, Banga P, Czinege Z, Sótonyi P Abstract Acute aortic catastrophes (AAC), mainly ruptured aneurysms and dissections, lead all other vascular conditions in morbidity and mortality, even if intervention occurs. The aim of our study was to give a descriptive overview of the demographic and pathological characteristics of AAC. Between 1994 and 2013, 80,469 autopsies were performed at Semmelweis University hospitals in Budapest. After collecting the autopsy reports we were able to create the AAC database upon which we conducted our analysis. We found 567 cases of AAC. The cause of death in 120 of them was cla...
Source: Pathology Oncology Research - June 16, 2020 Category: Pathology Authors: Pál D, Szilágyi B, Berczeli M, Szalay CI, Sárdy B, Oláh Z, Székely T, Rácz G, Banga P, Czinege Z, Sótonyi P Tags: Pathol Oncol Res Source Type: research

Immunohistochemical Investigation of Predictive Biomarkers for Mandibular Bone Invasion in Oral Squamous Cell Carcinoma.
Abstract The accurate preoperative determination of the extent of mandibular resection remains a challenge for the surgeons. The purpose of the present study was to immunohistochemically investigate predictive markers for histological bone invasion of oral squamous cell carcinoma (OSCC). The medical records of primary OSCC patients with mandibular bone contact in preoperative computed tomography scans between January 2003 and December 2017 were retrospectively reviewed and an immunohistochemical investigation was performed. Forty-five OSCC patients with mandibular bone contact radiographically were included in thi...
Source: Pathology Oncology Research - June 10, 2020 Category: Pathology Authors: Gibo T, Yamada SI, Kawamoto M, Uehara T, Kurita H Tags: Pathol Oncol Res Source Type: research

PD-L1 Expression in Small Cell and Large Cell Neuroendocrine Carcinomas of Lung: an Immunohistochemical Study with Review of Literature.
Abstract High-grade neuroendocrine tumors (HGNET) have distinctive tumor biology/behaviour. Newer modalities of treatment (immunotherapy) for them have been included in recent NCCN guidelines. Detection of programmed death receptor-ligand 1 (PD-L1) expression by immunohistochemistry have made easy identification of patients eligible for immunotherapy. We aimed to ascertain expression of PD-L1 on small cell and large cell neuroendocrine carcinomas of lung and review existing literature. Eighty-five cases of HGNET lung (primary/metastatic), were retrieved and reviewed. Immunostaining for PD-L1 using clone SP263 was ...
Source: Pathology Oncology Research - June 6, 2020 Category: Pathology Authors: Guleria P, Kumar S, Malik PS, Jain D Tags: Pathol Oncol Res Source Type: research

Detection of Low-level EGFR c.2369 C   >  T (p.Thr790Met) Resistance Mutation in Pre-treatment Non-small Cell Lung Carcinomas Harboring Activating EGFR Mutations and Correlation with Clinical Outcomes.
Detection of Low-level EGFR c.2369 C > T (p.Thr790Met) Resistance Mutation in Pre-treatment Non-small Cell Lung Carcinomas Harboring Activating EGFR Mutations and Correlation with Clinical Outcomes. Pathol Oncol Res. 2020 Jun 06;: Authors: Ye L, Mesbah Ardakani N, Thomas C, Spilsbury K, Leslie C, Amanuel B, Millward M Abstract Increasing evidence points to the presence of low-level de novo T790M mutations in patients with non-small cell lung carcinoma (NSCLC) harboring activating EGFR mutations. We utilized digital PCR (dPCR), a highly sensitive gene mutation detection method, to dete...
Source: Pathology Oncology Research - June 6, 2020 Category: Pathology Authors: Ye L, Mesbah Ardakani N, Thomas C, Spilsbury K, Leslie C, Amanuel B, Millward M Tags: Pathol Oncol Res Source Type: research

Power Doppler Sonography (PDS) and Modified TRUS Systematic Biopsies - Can this Combination Adequately Replace Multiparametric Prostate Magnetic Resonance Imaging (mp-MRI) in Candidates for Re Biopsies Who cannot Undergo mp-MRI.
Abstract The MRI targeted biopsy (MRI-TBx) may increase the detection rate of clinically significant cancer (csPCa) in candidates for re-biopsy. However, there will be several patients in whom MRI is contraindicated. In this retrospective study we assessed the ability of combination of PDS guided biopsies (PDS-TBx) and modified SBx to substitute MRI-TBx. 154 men with persistently elevated PSA were referred for re-biopsy. Our protocol included a combination of MRI-TBx, DPS-TBx and modified SBx with additional biopsies from anterior lateral horns and anterior aspects of apex. MRI findings were defined as suspicious ...
Source: Pathology Oncology Research - June 5, 2020 Category: Pathology Authors: Kravchick S, Cherniavsky E, Peled R, Cytron S, Verhovsky G Tags: Pathol Oncol Res Source Type: research

Prognosis Trend of Grade 2 Endometrioid Endometrial Carcinoma: Toward Grade 1 or 3?
In this study, we aim to investigate whether the oncologic outcomes of grade 2 endometrioid endometrial carcinomas trend towards grade 1 or 3 tumors. Patients' records and pathological reports were reviewed retrospectively and eligible patients with endometrioid endometrial carcinoma were determined and distributed into 3 groups according to their 1988 International Federation of Gynecology and Obstetrics (FIGO) grade. Groups' characteristics and oncologic outcomes were compared. Differences between grades were tested with z-test and adjusted by Bonferroni method. Kaplan-Meier method was performed for the survival analysis...
Source: Pathology Oncology Research - June 2, 2020 Category: Pathology Authors: Khatib G, Gulec UK, Guzel AB, Bagir E, Paydas S, Vardar MA Tags: Pathol Oncol Res Source Type: research