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Specialty: Biochemistry
Cancer: Breast Carcinoma

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Total 18 results found since Jan 2013.

Syndecan-1 and -4 influence Wnt signaling and cell migration in human breast cancers
This study examined syndecan-1 (SDC1) and syndecan-4 (SDC4; SDC1/4) in breast cancer (BC) in vitro cell models and their role in tumorigenesis. Gene expression of HSPG core proteins, biosynthesis and modification enzymes along with Wnt/FGF morphogen pathway components were examined following inhibition of SDC1 and SDC4 via small interfering RNA (siRNA), and enhancement of HSPGs via addition of heparin and FGF. siRNAs knockdowns (KDs) were performed in the MCF-7 (lowly invasive and poorly metastatic) and the MDA-MB-231 (highly invasive and metastatic) human BC cell lines. Significantly decreased gene expression of SDC1 and ...
Source: Biochimie - February 20, 2022 Category: Biochemistry Authors: Son H Pham Kaylah Pratt Rachel K Okolicsanyi Lotta E Oikari Chieh Yu Ian W Peall Km Taufiqul Arif Te-Arn Chalmers Martina Gyimesi Lyn R Griffiths Larisa M Haupt Source Type: research

Neuraminidase 1 regulates proliferation, apoptosis and the expression of Cadherins in mammary carcinoma cells.
Abstract The link between Neuraminidase 1 (Neu1) and cancer development has been highlighted in numerous studies. In an effort to understand the role of Neu1 in mammary carcinoma cells, we evaluated the effect of Neu1 on controlling cell proliferation and apoptosis, as well as regulating the expression of cadherins. By blocking the activity of Neu1 with oseltamivir phosphate or using siRNA to silence the Neu1 protein, we observed suppression of cell growth in MCF-7 and MDA-MB-231 cells. Enhanced cleaved caspase 3 expression was demonstrated in breast cancer cells treated with oseltamivir phosphate or in Neu1 knock...
Source: Molecular and Cellular Biochemistry - September 11, 2019 Category: Biochemistry Authors: Thulasiraman P, Kerr K, McAlister K, Hardisty S, Wistner A, McCullough I Tags: Mol Cell Biochem Source Type: research

OTOR in breast carcinoma as a potent prognostic predictor correlates with cell proliferation, migration and invasion.
This study aimed to investigate clinicopathologic significance of OTOR in breast infiltrating ductal carcinoma (IDC) with high metastasis and unveil its biological function in BC. OTOR was highly overexpressed in BC tissues and cells compared with normal samples. OTOR overexpression was associated with a few of clinicopathologic characteristics and worse overall survival (OS) of IDC breast patients. Silencing of OTOR using siRNA tool impeded BC cells proliferation, migration and invasion by CCK-8, colony formation, wound-healing and transwell assays, which might be regulated by mitogen-activated protein kinase/extracellula...
Source: Biochemistry and Cell Biology - March 20, 2019 Category: Biochemistry Authors: Wang YF, Han J Tags: Biochem Cell Biol Source Type: research

EZH2 promotes cell proliferation by regulating the expression of RUNX3 in laryngeal carcinoma.
Abstract Enhancer of zeste homolog 2 (EZH2) is a highly conserved histone methyltransferase, which is overexpressed in different types of cancers such as breast and prostate cancer. It is reported that EZH2 can directly down-regulate RUNX3 by increasing histone H3 methylation. However, the role of EZH2 in the development and progression of laryngeal carcinoma has not yet been investigated, and the relationship between EZH2 and RUNX3 in laryngeal carcinoma is rarely reported. The current study aims to determine the role of EZH2 in the progression of laryngeal carcinoma, and investigate the interaction between EZH2 ...
Source: Molecular and Cellular Biochemistry - August 9, 2017 Category: Biochemistry Authors: Lian R, Ma H, Wu Z, Zhang G, Jiao L, Miao W, Jin Q, Li R, Chen P, Shi H, Yu W Tags: Mol Cell Biochem Source Type: research

17beta-hydroxysteroid dehydrogenase type 5 is negatively correlated to apoptosis inhibitor GRP78 and tumor-secreted protein PGK1, and modulates breast cancer cell viability and proliferation
Publication date: Available online 27 April 2017 Source:The Journal of Steroid Biochemistry and Molecular Biology Author(s): Dan Xu, Juliette A. Aka, Ruixuan Wang, Sheng-Xiang Lin 17beta-hydroxysteroid dehydrogenase type 5 (17β-HSD5) is an important enzyme associated with sex steroid metabolism in hormone-dependent cancer. However, reports on its expression and its prognostic value in breast cancer are inconsistent. Here, we demonstrate the impact of 17β-HSD5 expression modulation on the proteome of estrogen receptor-positive (ER+) breast cancer cells. RNA interference technique (siRNA) was used to knock down 17β-HSD5 ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - April 29, 2017 Category: Biochemistry Source Type: research

Oxidative stress and glyoxalase I activity mediate dicarbonyl toxicity in MCF-7 mamma carcinoma cells and a tamoxifen resistant derivative
Conclusions Dicarbonyl toxicity was mediated by oxidative stress and GLO1 activity determines aldehyde toxicity in tamoxifen resistant cells. General Significance Glyoxalases might be predictive biomarkers for tamoxifen resistance and a putative target for the treatment of tamoxifen resistant breast cancer patients. Graphical abstract
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 22, 2016 Category: Biochemistry Source Type: research

Oxidative stress and glyoxalase-1 activity mediate dicarbonyl toxicity in MCF-7 mamma carcinoma cells and a tamoxifen-resistant derivative
Conclusions Dicarbonyl toxicity was mediated by oxidative stress and GLO-1 activity determines aldehyde toxicity in tamoxifen resistant cells. General Significance. Glyoxalases might be predictive biomarkers for tamoxifen resistance and a putative target for the treatment of tamoxifen resistant breast cancer patients. Graphical abstract
Source: Biochimica et Biophysica Acta (BBA) General Subjects - March 12, 2016 Category: Biochemistry Source Type: research

Oxidative stress and glyoxalase-1 activity mediate dicarbonyl toxicity in MCF-7 mamma carcinoma cells and a tamoxifen resistant derivative.
CONCLUSIONS: Dicarbonyl toxicity was mediated by oxidative stress and GLO-1 activity determines aldehyde toxicity in tamoxifen resistant cells. GENERAL SIGNIFICANCE: Glyoxalases might be predictive biomarkers for tamoxifen resistance and a putative target for the treatment of tamoxifen resistant breast cancer patients. PMID: 26971627 [PubMed - as supplied by publisher]
Source: Biochimica et Biophysica Acta - March 10, 2016 Category: Biochemistry Authors: Nass N, Sel S, Ignatov A, Roessner A, Kalinski T Tags: Biochim Biophys Acta Source Type: research

Silencing SOX2 Expression by RNA Interference Inhibits Proliferation, Invasion and Metastasis, and Induces Apoptosis through MAP4K4/JNK Signaling Pathway in Human Laryngeal Cancer TU212 Cells
SRY (sex determining region Y)-box 2 (SOX2) plays an important role in tumor cell metastasis and apoptosis. Laryngeal squamous cell carcinoma (LSCC), responsible for 1.5% of all cancers, is one of the most common head and neck malignancies. Accumulating evidence shows that SOX2 is overexpressed in several human tumors, including lung cancer, esophageal carcinoma, pancreatic carcinoma, breast cancer, ovarian carcinoma and glioma. Our study aimed to investigate the silencing effects of SOX2 expression using RNA interference (RNAi) on various biological processes in laryngeal cancer TU212 cells, including proliferation, apopt...
Source: Journal of Histochemistry and Cytochemistry - August 25, 2015 Category: Biochemistry Authors: Yang, N., Wang, Y., Hui, L., Li, X., Jiang, X. Tags: Articles Source Type: research

AP4 activates cell migration and EMT mediated by p53 in MDA-MB-231 breast carcinoma cells.
Abstract Tumor metastasis is the primary cause of mortality in most cancer patients. Before disassociation from the tumors, most of malignant tumor cells undergo the epithelial-mesenchymal transition to break away from the adhesions between the cells and the surrounding extracellular matrix. Recently, activating enhancer-binding protein (AP4) has been shown to be a mediator of EMT in colorectal cancer and high level of AP4 correlates with poor prognosis in cancer patients. It has been found that AP4 upregulates the genes involved in EMT and cell proliferation in colorectal cancer cells and that the aggressive hum...
Source: Molecular and Cellular Biochemistry - June 3, 2015 Category: Biochemistry Authors: Chen S, Chiu SK Tags: Mol Cell Biochem Source Type: research

Hyper-dependence of breast cancer cell types on the nuclear transporter Importin β1.
Abstract We previously reported that overexpression of members of the Importin (Imp) superfamily of nuclear transporters results in increased nuclear trafficking through conventional transport pathways in tumour cells. Here we show for the first time that the extent of overexpression of Impβ1 correlates with disease state in the MCF10 human breast tumour progression system. Excitingly, we find that targeting Impβ1 activity through siRNA is>30 times more efficient in decreasing the viability of malignant ductal carcinoma cells compared to isogenic non-transformed counterparts, and is highly potent and tumour s...
Source: Biochimica et Biophysica Acta - May 7, 2015 Category: Biochemistry Authors: Kuusisto HV, Jans DA Tags: Biochim Biophys Acta Source Type: research