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Condition: Thrombosis

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Total 39135 results found since Jan 2013.

Novel hydroxybutyl chitosan nanoparticles for siRNA delivery targeting tissue factor inhibits proliferation and induces apoptosis in human vascular smooth muscle cells.
Authors: Wan K, Li J, Li D, Ge J, Wang Y, Li X, Guo Y, Guo J, Leng M, Wang P, An Y Abstract Chitosan, a polysaccharide isolated from shrimp and other crustacean shells, has been widely investigated for DNA and siRNA delivery. Despite substantial effort having been made to improve chitosan as a non‑viral gene delivery vector, the application is severely limited by its poor solubility under physiological conditions. Hydroxybutyl chitosan (HBC), a modified chitosan, is soluble under neutral conditions. Tissue factor (TF) is involved in the pathogenesis of cardiovascular diseases by promoting thrombus formation and i...
Source: Molecular Medicine Reports - October 28, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Generation of transgenic pigs carrying siRNA vector directed against human tissue factor expression
ConclusionsThe AVR is currently the bottleneck in porcine‐to‐primate organ xenotransplantation. Controlling TF‐induced coagulation might provide protective effects for porcine xenografts. Thus, TF knockdown pigs could be critical in producing the ultimate multi‐transgenic pig as organ donor for human patients. References[1] Toomey J, Kratzer K, Lasky N, Stanton J, Broze GJ. Targeted disruption of the murine tissue factor gene results in embryonic lethality. Blood. 1996; 88(5): 1583–1587.
Source: Xenotransplantation - April 19, 2014 Category: Transplant Surgery Authors: Hellen Ahrens, Björn Petersen, Doris Herrmann, Andrea Lucas‐Hahn, Petra Hassel, Maren Ziegler, Wilfried Kues, Heiner Niemann Tags: Program and Abstracts Source Type: research

Targeting A549 lung adenocarcinoma cell growth and invasion with protease‑activated receptor‑1 siRNA.
In conclusion, the present study demonstrated that the progression of A549 cells is able to be inhibited by knockdown of PAR1 expression. Efficient delivery of the specific siRNA targeting PAR1 may be used for further study in clinical cancer therapy. PMID: 24604247 [PubMed - as supplied by publisher]
Source: Molecular Medicine - March 6, 2014 Category: Molecular Biology Authors: Wu Z, Zeng Y, Zhong M, Wang B Tags: Mol Med Rep Source Type: research

siRNA down-regulation of FGA mRNA in HepG2 cells demonstrated that heterozygous abnormality of the Aα-chain gene does not affect the plasma fibrinogen level
Conclusions: Our results suggest that FGG mRNA levels limit fibrinogen expression in normal liver and HepG2 cells and that 50% reduction of FGA mRNA levels would not limit fibrinogen expression in normal liver and HepG2 cells.
Source: Thrombosis Research - February 15, 2013 Category: Hematology Authors: Yuka Takezawa, Kazuyuki Matsuda, Fumiko Terasawa, Mitsutoshi Sugano, Takayuki Honda, Nobuo Okumura Tags: Coagulation and Fibrinolysis Source Type: research

Titrating haemophilia B phenotypes using siRNA strategy: evidence that antithrombotic activity is separated from bleeding liability.
Abstract Haemophilia A and B are characterised by a life-long bleeding predisposition, and several lines of evidence suggest that risks of atherothrombotic events may also be reduced. Establishing a direct correlation between coagulation factor levels, thrombotic risks and bleeding propensity has long been hampered by an inability to selectively and specifically inhibit coagulation factor levels. Here, the exquisite selectivity of gene silencing combined with a gene knockout (KO) approach was used to define the relative contribution of factor IX (fIX) to thrombosis and primary haemostasis in the rat. Using a lipid...
Source: Thrombosis and Haemostasis - March 19, 2015 Category: Hematology Authors: Metzger JM, Tadin-Strapps M, Thankappan A, Strapps WR, Di Poetro M, Leander K, Zhang Z, Shin MK, Levorse J, Desai K, Xu Y, Lai K, Wu W, Chen Z, Cai TQ, Jochnowitz N, Bentley R, Hoos L, Zhou Y, Sepp-Lorenzino L, Seiffert D, Andre P Tags: Thromb Haemost Source Type: research

siRNA Mediated Knockdown of Tissue Factor Expression in Pigs for Xenotransplantation
Abstract Acute vascular rejection (AVR), in particular microvascular thrombosis, is an important barrier to successful pig‐to‐primate xenotransplantation. Here, we report the generation of pigs with decreased tissue factor (TF) levels induced by small interfering (si)RNA‐mediated gene silencing. Porcine fibroblasts were transfected with TF‐targeting small hairpin (sh)RNA and used for somatic cell nuclear transfer. Offspring were analyzed for siRNA, TF mRNA and TF protein level. Functionality of TF downregulation was investigated by a whole blood clotting test and a flow chamber assay. TF siRNA was expressed in all ...
Source: American Journal of Transplantation - March 23, 2015 Category: Transplant Surgery Authors: H. E. Ahrens, B. Petersen, D. Herrmann, A. Lucas‐Hahn, P. Hassel, M. Ziegler, W. A. Kues, U. Baulain, W. Baars, R. Schwinzer, J. Denner, D. Rataj, S. Werwitzke, A. Tiede, A. K. Bongoni, P. S. Garimella, A. Despont, R. Rieben, H. Niemann Tags: Brief Communication Source Type: research

Targeting of antithrombin in hemophilia A or B with investigational siRNA therapeutic fitusiran - results of the phase 1 inhibitor cohort.
CONCLUSIONS: Monthly fitusiran was generally well tolerated, lowered antithrombin levels from baseline, and resulted in improved thrombin generation. These preliminary results suggest that monthly fitusiran treatment may reduce bleeding episodes and improve QoL in participants with hemophilia A or B with inhibitors. PMID: 33587824 [PubMed - as supplied by publisher]
Source: Thrombosis and Haemostasis - February 15, 2021 Category: Hematology Authors: Pasi KJ, Lissitchkov T, Mamonov V, Mant T, Timofeeva M, Bagot C, Chowdary P, Georgiev P, Gercheva-Kyuchukova L, Madigan K, Nguyen H, Yu Q, Mei B, Benson CC, Ragni MV Tags: J Thromb Haemost Source Type: research

Heparin versus 0.9% sodium chloride locking for prevention of occlusion in central venous catheters in adults
CONCLUSIONS: Given the low-certainty evidence, we are uncertain whether intermittent locking with heparin results in fewer central venous catheter occlusions than intermittent locking with normal saline in adults. Low-certainty evidence suggests that heparin may have little or no effect on catheter patency duration. Although we found no evidence of differences in safety (CVC-related bloodstream infections, mortality, or haemorrhage), the combined studies were not powered to detect rare adverse events such as heparin-induced thrombocytopaenia. Further research conducted over longer periods would reduce the current uncertain...
Source: Cochrane Database of Systematic Reviews - July 18, 2022 Category: General Medicine Authors: Eduardo L ópez-Briz Vicente Ruiz Garcia Juan B Cabello Sylvia Bort-Mart í Rafael Carbonell Sanchis Source Type: research

Interventions for preventing venous thromboembolism in adults undergoing knee arthroscopy
CONCLUSIONS: There is a small risk that healthy adults undergoing KA will develop venous thromboembolism (PE or DVT). We found moderate- to low-certainty evidence of little or no benefit from LMWH, or rivaroxaban in reducing this small risk of PE or symptomatic DVT. The studies provided very low-certainty evidence that LMWH may reduce the risk of asymptomatic DVT compared to no prophylaxis, but it is uncertain how this directly relates to incidence of DVT or PE in healthy people undergoing KA. There is probably little or no difference in adverse effects (including major and minor bleeding), but data relating to these outco...
Source: Cochrane Database of Systematic Reviews - August 22, 2022 Category: General Medicine Authors: Carla Perrotta Jorge Chahla Gustavo Badariotti Jorge Ramos Source Type: research

Expanded polytetrafluoroethylene (ePTFE)-covered stents versus bare stents for transjugular intrahepatic portosystemic shunt in people with liver cirrhosis
CONCLUSIONS: Based on the small number of trials with insufficient sample size and events, and study limitations, we assessed the overall certainty of evidence in the predefined outcomes as low or very low. Therefore, we are uncertain which of the two interventions (ePTFE-covered stents or bare stents of the same diameter and ePTFE-covered stents versus bare stents of different stent diameters) is effective for the evaluated outcomes. None of the four trials reported data on health-related quality of life, and data on complications were either missing or rarely reported. We lack high-quality trials to evaluate the role of ...
Source: Cochrane Database of Systematic Reviews - August 2, 2023 Category: General Medicine Authors: Peng Zhu Sitong Dong Ping Sun Ajay P Belgaumkar Yi Sun Xiang Cheng Qichang Zheng Tong Li Source Type: research

Retrospective Derivation and Validation of an Automated Electronic Search Algorithm to Identify...
Conclusions: The derivation and validation of an electronic search strategy can accelerate the data abstraction process for research, quality improvement, and enhancement of patient care, while maintaining superb reliability compared to manual review....
Source: Applied Clinical Informatics - September 9, 2015 Category: Information Technology Source Type: research

Heparin versus 0.9% sodium chloride locking for prevention of occlusion in central venous catheters in adults
CONCLUSIONS: Given the low-certainty evidence, we are uncertain whether intermittent locking with heparin results in fewer central venous catheter occlusions than intermittent locking with normal saline in adults. Low-certainty evidence suggests that heparin may have little or no effect on catheter patency duration. Although we found no evidence of differences in safety (CVC-related bloodstream infections, mortality, or haemorrhage), the combined studies were not powered to detect rare adverse events such as heparin-induced thrombocytopaenia. Further research conducted over longer periods would reduce the current uncertain...
Source: Cochrane Database of Systematic Reviews - July 18, 2022 Category: General Medicine Authors: Eduardo L ópez-Briz Vicente Ruiz Garcia Juan B Cabello Sylvia Bort-Mart í Rafael Carbonell Sanchis Source Type: research

Catheter-directed therapies for the treatment of high risk (massive) and intermediate risk (submassive) acute pulmonary embolism
CONCLUSIONS: There is a lack of evidence to support widespread adoption of catheter-based interventional therapies for APE. We identified one small trial showing no clear differences between ultrasound-augmented catheter-directed thrombolysis with alteplase plus systemic heparinisation versus systemic heparinisation alone in all-cause mortality, major and minor haemorrhage rates, recurrent APE and length of hospital stay. Quality of life was not assessed. Multiple small retrospective case series, prospective patient registries and single-arm studies suggest potential benefits of catheter-based treatments, but they provide ...
Source: Cochrane Database of Systematic Reviews - August 8, 2022 Category: General Medicine Authors: John J Harvey Shiwei Huang Raman Uberoi Source Type: research