Concomitant assessment of rivaroxaban concentration and its impact on thrombin generation
Reliable assays to measure direct oral anticoagulant (DOAC) levels and their activity in critical situations are needed. Drug levels alone are not representative of the effect of DOACs on an individual's coagulation. We developed a technique that provides direct assessment of the global effect of rivaroxaban on the individual's coagulation in addition to plasma concentrations. (Source: Thrombosis Research)
Source: Thrombosis Research - October 22, 2019 Category: Hematology Authors: Saartje Bloemen, Suzanne Zwaveling, Fran çois Mullier, Jonathan Douxfils Tags: Full Length Article Source Type: research

First visualization of circulating neutrophil extracellular traps using cell fluorescence during human septic shock-induced disseminated intravascular coagulation
Disseminated intravascular coagulation (DIC) is a severe complication of septic shock. Polymorphonuclear neutrophils (PMNs) may play a key role in septic shock-induced DIC via the release of neutrophils extracellular traps (NETs). NETs capture invading pathogens, but also act as a pro-coagulant surface at the interface between immunity and thrombosis. During septic shock-induced DIC, neutrophil activation may result in excessive NET formation. Herein, we originally report the presence of circulating NETs in human blood during septic shock-induced DIC. (Source: Thrombosis Research)
Source: Thrombosis Research - October 22, 2019 Category: Hematology Authors: Laure Stiel, Caroline Mayeur-Rousse, Julie Helms, Ferhat Meziani, Laurent Mauvieux Tags: Full Length Article Source Type: research

Thalidomide prevents antibody-mediated immune thrombocytopenia in mice
This study aims to evaluate the effect of thalidomide on ITP mouse model. ITP mouse model was established through intraperitoneal injection of rat anti-mouse integrin GPIIb/CD41 immunoglobulin. (Source: Thrombosis Research)
Source: Thrombosis Research - October 22, 2019 Category: Hematology Authors: Mengdi Xu, Xiamin Wang, Xiaoqi Xu, Guangyu Wei, Wenyi Lu, Qi Luo, Xiaoqian Li, Yun Liu, Wen Ju, Zhenyu Li, Kailin Xu, Lingyu Zeng, Jianlin Qiao Tags: Full Length Article Source Type: research

Confirmed validation of an innovative PCR-assay without DNA extraction for multiplex diagnosis of factor V Leiden and prothrombin gene variants
Venous thromboembolic disease (VTE) is a major public health issue in developed countries with an estimated annual occurrence ranging from 1 to 2 per 1000 inhabitants and increased risk over lifetime [1]. The major occurrences of VTE are lower limbs thrombosis and pulmonary embolisms, but any vein can be affected by an inappropriate clot formation. Besides age, several risk factors have been reported, all belonging to the triad described in the 19th century by Rudolf Virchow, i.e. hypercoagulability, blood stasis and endothelial damage. (Source: Thrombosis Research)
Source: Thrombosis Research - October 22, 2019 Category: Hematology Authors: A. Desjonqu ères, A. Ménard, L. Detemmerman, C. Ternisien, M. Fouassier, B. Gillet, M.C. Béné, Y. Le Bris Tags: Letter to the Editors-in-Chief Source Type: research

Effect of rivaroxaban and dabigatran on platelet functions: In vitro study
Clinical benefit-risk balance of direct oral anticoagulants (DOAC) in atherothrombosis prevention differs between anti-Xa and anti-IIa molecules and their specific effect on platelet functions remains controversial. We hence investigated rivaroxaban and dabigatran effect on platelets in identical experimental conditions. (Source: Thrombosis Research)
Source: Thrombosis Research - October 22, 2019 Category: Hematology Authors: Georges Jourdi, Christilla Bachelot-Loza, Elisabeth Mazoyer, Sonia Poirault-Chassac, Jerome Duchemin, Michaela Fontenay, Pascale Gaussem Tags: Letter to the Editors-in-Chief Source Type: research

Clinical presentation and outcomes in elderly patients with symptomatic isolated subsegmental pulmonary embolism
Data are limited on clinical presentation and outcomes in elderly patients with acute symptomatic isolated subsegmental pulmonary embolism (SSPE). We compared clinical presentation, risk factors, processes of care, and outcomes between elderly patients with SSPE and patients with more proximal pulmonary embolism (PE). (Source: Thrombosis Research)
Source: Thrombosis Research - October 22, 2019 Category: Hematology Authors: Nina Stoller, Andreas Limacher, Marie M éan, Christine Baumgartner, Tobias Tritschler, Marc Righini, Jürg-Hans Beer, Nicolas Rodondi, Drahomir Aujesky Tags: Full Length Article Source Type: research

Assessment of genetic and non-genetic risk factors for venous thromboembolism in glioblastoma – The predictive significance of B blood group
Venous thromboembolism (VTE) is a common problem among patients with glioblastoma multiforme (GBM) and with some other cancers. Here, we evaluated genetic and non-genetic potential risk factors for VTE among GBM patients. (Source: Thrombosis Research)
Source: Thrombosis Research - October 22, 2019 Category: Hematology Authors: Menikae K. Heenkenda, Annika Malmstr öm, Malgorzata Lysiak, Munila Mudaisi, Charlotte Bratthäll, Peter Milos, Michael Strandeus, Lisa Åkesson, Peter Söderkvist, Srinivas Uppugunduri, Abdimajid Osman Tags: Full Length Article Source Type: research

The features of acquired thrombotic thrombocytopenic purpura occurring at advanced age
Acquired thrombotic thrombocytopenic purpura (aTTP) is a rare life-threatening thrombotic microangiopathy (TMA) affecting more frequently women of 30 –50 years of age. There is scarce information on the clinical features of aTTP occurring in the elderly.Our goal was to evaluate the impact of an elderly-onset disease on the expression, severity and management of aTTP. (Source: Thrombosis Research)
Source: Thrombosis Research - October 22, 2019 Category: Hematology Authors: Pasquale Agosti, Ilaria Mancini, Andrea Artoni, Barbara Ferrari, Silvia Pontiggia, Silvia Maria Trisolini, Luca Facchini, Flora Peyvandi, the Italian Group of TTP Investigators Tags: Full Length Article Source Type: research

Sex-specific alteration to α2-antiplasmin incorporation in patients with type 2 diabetes
Type 2 diabetes mellitus (T2DM) is associated with hypofibrinolysis and increased factor XIII-mediated α2-antiplasmin incorporation into the fibrin clot. It is unclear whether there are sex-related differences in α2-antiplasmin incorporation in relation to impaired clot lysis in T2DM. (Source: Thrombosis Research)
Source: Thrombosis Research - October 21, 2019 Category: Hematology Authors: Agata Hanna Bryk, Jakub Siudut, El żbieta Broniatowska, Zsuzsa Bagoly, Barbara Baráth, Éva Katona, Anetta Undas Tags: Full Length Article Source Type: research

Frequency of arterial thromboembolism in populations with malignancies: A systematic review
This study aims to systematically review the frequency of ATE in patients with cancer. (Source: Thrombosis Research)
Source: Thrombosis Research - October 21, 2019 Category: Hematology Authors: James Yu, Allen Li, Marissa Laureano, Mark Crowther Tags: Review Article Source Type: research

Short-term versus extended anticoagulant treatment for unprovoked venous thromboembolism: A survey on guideline adherence and physicians' considerations
In patients with unprovoked venous thromboembolism (VTE), anticoagulant treatment duration should be decided by weighing bleeding risk versus risk of recurrent VTE, considering patient's preference. Because both risks differ between individuals, this recommendation presumably leads to wide variation in clinical management. (Source: Thrombosis Research)
Source: Thrombosis Research - October 21, 2019 Category: Hematology Authors: Maria A. de Winter, Gabri ëlle C.P. Remme, Karin H.A.H. Kaasjager, Mathilde Nijkeuter Tags: Full Length Article Source Type: research

Acquired von Willebrand syndrome in patients on long-term left ventricular assist device support: Results of a Belgian center
Acquired von Willebrand syndrome (aVWS) in patients treated with a left ventricular assist device (LVAD) is characterized by a loss of high molecular weight (HMW) von Willebrand factor (VWF) multimers which often coincides with an impaired VWF function reflected by a decreased VWF collagen-binding capacity and decreased binding of VWF to the platelet glycoprotein (GP) Ib receptor [1]. The high shear stress generated by the device leads to unfolding of the HMW VWF multimers and cleavage of VWF by ADAMTS13 (A Disintegrin And Metalloprotease with a ThromboSpondin type 1 motif, member 13) [2]. (Source: Thrombosis Research)
Source: Thrombosis Research - October 21, 2019 Category: Hematology Authors: Shannen J. Deconinck, Claudia Tersteeg, Els Bailleul, Leen Delrue, Nele Vandeputte, Inge Pareyn, Hans Deckmyn, Simon F. De Meyer, Karen Vanhoorelbeke, Marc Vanderheyden Tags: Letter to the Editors-in-Chief Source Type: research

Severe persistent heparin-induced thrombocytopenia in a renal transplant patient
Heparin is commonly used in the renal transplant setting but heparin-induced thrombocytopenia (HIT) a serious adverse reaction to heparin appears to be infrequent in this patient population [1 –3]. A subset of HIT antibodies considered “pathogenic” activate platelets in functional assays like the serotonin release assay (SRA) and can be distinguished from “benign” antibodies commonly seen in clinical practice that are positive in PF4/Polyanion ELISAs (PF4 ELISA) but are not plat elet-activating [4]. (Source: Thrombosis Research)
Source: Thrombosis Research - October 21, 2019 Category: Hematology Authors: Divya Chawla, Ehab Saad, Talal Khairi, Anand Padmanabhan Tags: Letter to the Editors-in-Chief Source Type: research

Incidence and characteristics of venous thromboembolism in Asian patients with primary central nervous system lymphoma undergoing chemotherapy
Venous thromboembolism (VTE) may lead to diminished quality of life and ultimately worse prognosis in cancer patients, but there is limited data on the incidence, risk factors and mortality impact of VTE in Asian primary central nervous system lymphoma (PCNSL) patients. (Source: Thrombosis Research)
Source: Thrombosis Research - October 20, 2019 Category: Hematology Authors: Ja Min Byun, Junshik Hong, Sung-Soo Yoon, Youngil Koh, Chan-Young Ock, Tae Min Kim, Ji Hyun Lee, Sung-Hyun Kim, Jeong-Ok Lee, Soo-Mee Bang, Ho-Young Yhim, Sung Hwa Bae, Yeung-Chul Mun, Doyeun Oh, Korean Thrombosis and Hemostasis Working Party of Korean So Tags: Full Length Article Source Type: research

Higher CD56+ or CD2+ lymphocyte percentage predicts poor steroid response in patients with immune thrombocytopenia
This study evaluated the roles of subgroup lymphocytes from peripheral blood in ITP adults with different treatment response. (Source: Thrombosis Research)
Source: Thrombosis Research - October 20, 2019 Category: Hematology Authors: Yi-Feng Wu, Wei-Han Huang, Ming-Huei Gu, Siao-Ru Lin, Sung-Chao Chu, Tso-Fu Wang, Chi-Cheng Li Tags: Full Length Article Source Type: research

Patient experience of pregnancy-related venous thrombosis: A phenomenological study
This study used a descriptive phenomenological approach to explore the lived experience of women diagnosed with VTE in pregnancy and the perinatal period. The study setting was a London, university affiliated hospital. The sample consisted of nine women with deep vein thrombosis or pulmonary embolism in pregnancy or up to six weeks post-partum, diagnosed in the last two years. Semi-structured interviews were undertaken using an interview guide. Interviews were transcribed and analysed using phenomenological descriptive analysis. (Source: Thrombosis Research)
Source: Thrombosis Research - October 20, 2019 Category: Hematology Authors: Emma Gee, Lara Roberts, Roopen Arya, Vicki Tsianakas Tags: Full Length Article Source Type: research

Podoplanin expression on endothelial cells promotes superficial erosive injury and thrombus formation in rat carotid artery: Implications for plaque erosion
Atherosclerotic plaque erosion, a morphological pattern of plaque disruption, is characterized by a denuded plaque surface and thrombus formation, and defined by the lack of disruption of the fibrous cap and exposure of necrotic core. The morphologic characteristics include an abundance of smooth muscle cells (SMCs) and proteoglycan matrix and disruption of surface endothelium. Plaque erosion contains relatively few inflammatory cells compared with plaque rupture [1]. Although many mechanisms including disturbed blood flow, toll-like receptor signaling, leukocyte activation and modification of sub-endothelial matrix have b...
Source: Thrombosis Research - October 20, 2019 Category: Hematology Authors: Eiji Furukoji, Atsushi Yamashita, Kensuke Nakamura, Toshinori Hirai, Yujiro Asada Tags: Letter to the Editors-in-Chief Source Type: research

Mutation of Factor IX Cys178 is intolerant and may cause severe hemophilia B
Hemophilia B (HB, OMIM: 300746) is a rare X-linked recessive hemorrhagic disorder caused by the deficiency of coagulation Factor IX (FIX), with an estimated prevalence of 5.7 cases per 100,000 births [1]. The F9 gene, located on Xq27.1, comprises 8 exons and encodes the vitamin K-dependent FIX, a 461 amino acid precursor which is further cleaved and secreted into plasma as a mature, 415-residue activated protein. Upon activation, coagulation factor VIII (FVIII) and FIX form a tenase complex to activate coagulation factor X (FX) and the following coagulation pathway. (Source: Thrombosis Research)
Source: Thrombosis Research - October 20, 2019 Category: Hematology Authors: Xiong Wang, Linna Gao, Na Shen, Aiguo Liu, Yanjun Lu, Qun Hu Tags: Letter to the Editors-in-Chief Source Type: research

Global coagulation assays in patients with multiple myeloma and monoclonal gammopathy of unknown significance
Both multiple myeloma and monoclonal gammopathy of undetermined significance (MGUS) are associated with increased venous thromboembolism (VTE) and arterial thrombosis [1]. The thrombogenicity of myeloma is multi-fold with current VTE risk stratification focusing predominantly on clinical factors. Key to augmenting our clinical risk assessment model to identify patients who may benefit from thromboprophylaxis is a predictive biomarker. Current coagulation testing are inadequate as they measure only the time to clot formation, making them poor indicators of a hypercoagulable state. (Source: Thrombosis Research)
Source: Thrombosis Research - October 20, 2019 Category: Hematology Authors: Hui Yin Lim, Rowena Brook, Brintha Krishnamoorthi, Mark Tacey, Teresa Leung, Geoffrey Donnan, Harshal Nandurkar, Prahlad Ho Tags: Letter to the Editors-in-Chief Source Type: research

Selective inhibition of Panx1 channels decreases hemostasis and thrombosis in vivo
Hemostasis is a tightly regulated physiological process to rapidly induce hemostatic plugs at sites of vascular injury. Inappropriate activation of this process may lead to thrombosis, i.e. pathological blood clot formation in uninjured vessels or on atherosclerotic lesions. ATP release through Pannexin1 (Panx1) membrane channels contributes to collagen-induced platelet aggregation in vitro. (Source: Thrombosis Research)
Source: Thrombosis Research - October 18, 2019 Category: Hematology Authors: Filippo Molica, Merlijn J. Meens, Graziano Pelli, Aur élie Hautefort, Yalin Emre, Beat A. Imhof, Pierre Fontana, Eliana Scemes, Sandrine Morel, Brenda R. Kwak Tags: Full Length Article Source Type: research

P1. Abstract Title: Markers of Coagulation as Potential Indicators for the Diagnosis of Sepsis
The emergency department (ED) is an important venue for early diagnosis of sepsis; however, it remains a clinical challenge due to its heterogenous presentation and lack of a definitive biomarker. Neutrophil extracellular trap formation, with the release of cell free DNA (cfDNA) and activation of the coagulation cascade, and inhibition of the anticoagulant Protein C (PC) through extracellular histone, have been shown to be important in sepsis prognosis. It is unknown whether these or other molecules important in the interface between infection and coagulation, might also be diagnostic. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: J. Arora, W. Faidi, R. Gregoris, A. Fox-Robichaud Tags: Poster Presentations Source Type: research

P2. Abstract Title: Delayed Time to Emergency Hip Surgery in Patients Taking Oral Anticoagulants
Surgical intervention within 48 hours of admission in patients with acute hip fractures has been associated to lower morbidity and mortality. Patients anticoagulated with Vitamin K Antagonists (VKAs) have longer time to corrective surgery than those not anticoagulated. Sparse data exists on time to surgery (TTS) in patients taking direct oral anticoagulants (DOACs). The aim of this study is to establish TTS among non-anticoagulated and anticoagulated patients taking either VKA or DOAC. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: T. Cafaro, M. Koolian, C. Simard, V. Tagalakis Source Type: research

OC1. Abstract Title: In-hospital Mortality and Morbidity among Patients Presenting with Intracranial Hemorrhage: A Single Center's Experience with Vitamin K Antagonists and the Direct Oral Anticoagulants
Oral anticoagulants are the preferred therapy for the treatment of venous thromboembolism and for stroke prevention among patients with atrial fibrillation. Given their widespread use, clinicians must balance efficacy of anticoagulation with their associated bleeding risks. Specifically, intracranial hemorrhage (ICH) is the most feared complication as this form of bleeding has the highest mortality and morbidity. To date, clinical trials suggest a lower incidence of ICH and better safety profile among patients prescribed the direct oral anticoagulants (DOACs) compared with traditional vitamin k antagonists (VKAs). (Source:...
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: C. Cipkar, S. Srinathan, P. Chiang, L. Castellucci Tags: Oral Communications Source Type: research

P3. Abstract Title: A Point-of-Care Assay for Measuring Thrombin Activity in Finger-Prick Whole Blood
At sites of vessel injury, thrombin acts as the central mediator of coagulation through catalyzing fibrin clot formation and platelet activation. Thus, numerous commercially available small molecule chromogenic and fluorogenic substrates have been developed to evaluate thrombin activity. These substrates are limited in that they detect both active thrombin and thrombin bound to its inhibitor α2-macroglobulin, have limited specificity for thrombin and can only be used in plasma samples because of signal quenching. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: Y. Dai, P. Gross Source Type: research

P4. Abstract Title: Prevalence of Pulmonary Embolism in Patients Accessing the Emergency Department, Reported by Country of Study: A Systematic Review and Meta-Analysis
Pulmonary embolism (PE) has an incidence of 0.75-2.69 cases per 1,000 individuals in Western Europe and North America (NA), with no significant differences between these two regions. In the United States, there is evidence of PE overdiagnosis, with increased incidence of PE over time and minimal changes in the mortality rate. It is possible that this tendency towards overdiagnosis (and over-testing) is different in NA and Europe. Greater tendency toward overtesting would imply a lower prevalence of PE among suspects and a lower diagnostic yield of imaging techniques. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: F. Germini, S. Zarabi, M. Eventov, M. Turcotte, K. Rigg, M. Li, K. De Wit Source Type: research

P5. Abstract Title: Characterization of Unfractionated Heparin Infusions in a Comprehensive Stroke Centre
Stroke patients with arterial or venous intraluminal thrombus (ILT) are at high risk of stroke recurrence and embolic or hemorrhagic complications. Treatment with anticoagulation remains challenging in this group of patients due to onset of action, efficacy, and risk of hemorrhage. Traditionally, unfractionated heparin (UFH) has been used due to its short half-life in the context of risk of hemorrhagic transformation of the infarct zone. Heparin nomograms and associated bolus dosing are not standardized in clinical practice across centres and patients can exhibit long epochs of time under or over the therapeutic PTT range....
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: H. Khosravani, P. Pham, C. Shen, C. Wong Source Type: research

P6. Abstract Title: Risks and Benefits of Antithrombotic Therapy in Polycythemia Vera: A Systematic Review
Thrombosis is the major cause of morbidity and mortality in patients with polycythemia vera (PV). Aspirin, and phlebotomy with or without cytoreductive therapy are key components of PV management to reduce thrombotic risk. However, patients with PV are also at risk for hemorrhagic complications, especially in the context of thrombocytosis and acquired Von Willebrand factor deficiency. The net benefit of antithrombotic therapy for primary and secondary thromboprophylaxis is uncertain. A systematic review conducted in 2013 identified 2 randomized control trials (RCTs) and concluded that low-dose aspirin does not increase the...
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: M. Laureano, L. Goodlife, D. Chu, D. Siegal Source Type: research

OC2. Abstract Title: Comparison of Two Clinical Scales to Assess the Post-Thrombotic Syndrome: Secondary Analysis of a Multicenter Randomized Trial of Pharmacomechanical Catheter-Directed Thrombolysis for Deep Vein Thrombosis
Post-thrombotic syndrome (PTS) occurs in 20-50% of patients following proximal deep vein thrombosis (DVT), despite anticoagulation treatment. The International Society on Thrombosis and Haemostasis recommends using the Villalta scale to standardize the diagnosis of PTS and to quantify its severity. However, many investigators use the Venous Clinical Severity Score (VCSS) to assess PTS. Different to the Villalta score, the VCSS was developed as a measure for chronic venous disease and not PTS specifically. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: A. Lee, C.-S. Gu, S. Vedantham, C. Kearon, M. Blostein, S. Kahn Source Type: research

P7. Abstract Title: Mapping the Interactome of the Metalloprotease ADAMTS13 in Plasma using In-Vitro BioID
Von Willebrand Factor (VWF) is a multimeric plasma protein that tethers platelets to sites of vascular damage, and is an important initiator of blood clotting. ADAMTS13 is a metalloprotease that cleaves VWF to regulate its platelet binding activity. Decreased activity of ADAMTS13 leads to larger VWF multimers resulting in pathological blood clots, such as thrombotic thrombocytopenic purpura (TTP) and stroke. Unlike other circulating proteases, ADAMTS13 is secreted as an active enzyme and is resistant to all known natural protease inhibitors. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: H. Madarati, C. Kretz Source Type: research

P8. Abstract Title: Inpatient Inherited Thrombophilia Testing a Multicenter Retrospective Review of Practice Patterns and Indications
Hereditary thrombophilia testing identifies genetic conditions that can predispose patients to an increased risk of venous thromboembolism (VTE); these conditions include Factor V Leiden, Factor II G20210A gene mutation, protein C deficiency and protein S deficiency. Testing as an inpatient can be challenging as acute thrombosis and anticoagulation can confound the study results. As a result, major society guidelines do not recommend routine hereditary thrombophilia testing in an acute setting. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: F. Manji, X. Jiang, D. Suryanarayan Source Type: research

P9. Abstract Title: Systolic Blood Pressure and Mortality in Acute Symptomatic Pulmonary Embolism
The optimal cut-off for systolic blood pressure (SBP) to define high-risk pulmonary embolism (PE) remains to be defined. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: A. Quezada, M. Porres Aguilar, D. Jim énez, B. Bikdeli, L. Moores, M. Aramberri, J. Lima, A. Ballaz, R. Yusen, M. Monreal Source Type: research

P10. Abstract Title: The Diagnostic Performance of a Non-Emergency Department-Based Deep Vein Thrombosis Ambulatory Care Pathway
Ambulatory management of isolated acute deep vein thrombosis (DVT) is the standard of care in most patient populations, and in clinical practice is mostly initiated in the emergency department (ED). However, patients referred to the ED with suspected DVT often experience unnecessary delays to diagnosis and subsequent long stays to determine appropriate treatment and follow up care. We implemented a DVT ambulatory care pathway that does not include the ED for referred patients with suspected isolated DVT that begins with pre-test risk stratification in the community and subsequent algorithm-based diagnostic work-up, treatme...
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: P.-M. Sene, D. Yahiaoui, M. Koolian, V. Tagalakis Source Type: research

OC3. Abstract Title: Enhanced VTE Risk Stratification in Ambulatory Patients with Cancer
Ambulatory cancer patients initiating chemotherapy are at increased risk of venous thromboembolism (VTE). The Khorana score (0  = low, 1-2 = intermediate, 3-6 = high) helps clinicians to stratify these patients according to their underlying risk of VTE. The CATS score, which incorporates additional points for elevated biomarkers (d-dimer, p-selectin), may enhance VTE risk stratification by adding up to two addit ional points to the Khorana score. We sought to evaluate the incidence of VTE in patients with a Khorana score of ≥ 2 and elevated biomarkers. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: J. Shaw, R. Mallick, M. Carrier, A. Ilich, N. Key, P. Wells Source Type: research

OC4. Abstract Title: Thrombus Stability Explains the Factor V Leiden Paradox: A Mouse Model
Factor V Leiden (FVL) is an inherited autosomal dominant condition that is identified in 20%-50% of patients with venous thromboembolic diseases and 5-8% of the population in Canada. People with FVL are often called hypercoagulable, and have a five-fold increase in the risk of venous thrombosis. There is a low prevalence of FVL among patients with fatal pulmonary embolism (PE) and a higher incidence of deep vein thrombosis (DVT) than PE in patients with FVL. This has been referred to as the FVL paradox in thrombosis. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: S. Shaya, P. Gross, R. Westrick Source Type: research

P11. Abstract Title: Investigating the resistance of ADAMTS13 towards Alpha 2-Macroglobulin
ADAMTS13 is a metalloprotease that regulates the platelet-capturing capacity of von Willebrand factor. ADAMTS13 is secreted in its active form and has a circulating half-life of 4-7 days despite the presence of relatively high concentrations of inhibitors known to target other members of the ADAMTS protease family. This suggests that ADAMTS13 is resistant to inhibition by natural protease inhibitors, such as Alpha 2-Macroglobin (A2M) and tissue inhibitors of metalloproteinases (TIMPs). ADAMTS13 is known to adopt a closed conformation whereby C-terminal CUB domains bind to the proximal SPACER domain. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: K. Singh, H. Madarati, T. Sparring, C. Kretz Source Type: research

P12. Abstract Title: The Effects of Glycosaminoglycans on DNasel-Mediated Degradation of Neutrophil Extracellular Traps and DNA-Histone Complexes
Neutrophil extracellular traps (NETs), released from activated neutrophils, have been implicated in contributing to micro- and macro-vascular thrombosis. The major structural component of NETs is nucleosomes, which consists of DNA wrapped around a core histone octamer containing two copies each of histone H2A, H2B, H3, and H4. The release of NETs occurs via a unique cell death pathway (termed NETosis), which requires the decondensation of chromatin by PAD4. PAD4 is a nuclear enzyme that converts specific arginine residues to citrulline on histones, thereby reducing the positive charge of histones. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: S. Sohrabipour, V. De Souza, N. Sharma, P. Law Source Type: research

P13. Abstract Title: Deep Mutational Scan of VWF to Define Mutations that Enhance or Impair Secretion
Von Willebrand Factor (VWF) is a multimeric glycoprotein that recruits platelets to sites of blood vessel injury and serves as a molecular chaperone for coagulation factor VIII (fVIII). Plasma VWF levels are a known risk factor for various thrombotic disorders including VTE, myocardial infarction, and stroke. GWAS studies have identified genetic variants in VWF that contribute to increased and decreased circulating VWF antigen levels. However, it is currently not known why some variants result in high VWF levels while others cause low VWF levels in circulation. (Source: Thrombosis Research)
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: T. Sparring, C. Kretz, K. Singh, H. Madarati Source Type: research

P14. Abstract Title: Modulating Fibrinolysis using siRNA against Coagulation Factor XIII
High specificity, easy reversibility, and a half-life on the order of weeks; these are all major advantages that gene therapy has over traditional anticoagulants, add to that a strategy that weakens clots rather than preventing clotting altogether and prophylaxis of thrombosis may become much safer. Currently, anticoagulant drugs are used as a preventative measure for people susceptible to developing disease causing thrombi (which manifest as heart attacks, strokes, and pulmonary embolism), but they require frequent administration and their strict inhibition of clotting increases the risk of uncontrolled bleeding. (Source:...
Source: Thrombosis Research - October 1, 2019 Category: Hematology Authors: A. Strilchuk, E. Conway, E. Pryzdial, P. Cullis, C. Kastrup Source Type: research

Platelet and white blood cell count are independently associated with sarcopenia: A nationwide population-based study
Sarcopenia is attracting increasing attention due to its harmful impacts on health. Chronic inflammation is proposed to be a major cause of sarcopenia. Here, we aimed to identify whether white blood cell (WBC) and platelet count have independent roles in sarcopenia occurrence. (Source: Thrombosis Research)
Source: Thrombosis Research - September 10, 2019 Category: Hematology Authors: Hye Sun Lee, Il-Hyun Koh, Hyoung Sik Kim, Yu-Jin Kwon Tags: Full Length Article Source Type: research

Clinical and laboratory diagnosis of rare coagulation disorders (RCDs)
Rare coagulation disorders (RCDs) are a group of diseases due to coagulation factors deficiency leading to life-long bleeding diathesis. The diagnosis of RCDs is challenging due to the limited knowledge of these disorders and the large heterogeneity of their bleeding patterns. The clinical symptoms of RCDs are extremely diverse in terms of bleeding type, site, severity, age at onset, and duration. The strength of the association between clotting factor activity level in plasma and clinical symptoms is also variable within each RCD. (Source: Thrombosis Research)
Source: Thrombosis Research - September 7, 2019 Category: Hematology Authors: Marzia Menegatti, Roberta Palla Tags: Full Length Article Source Type: research

Extracellular vesicles derived from pancreatic cancer cells BXPC3 or breast cancer cells MCF7 induce a permanent procoagulant shift to endothelial cells
The endothelium could be a potential target of cancer cell derived extracellular vesicles (CaCe-dEV). We investigated in vitro the effect of CaCe-dEV on the hemostatic balance of endothelial cells. Extracellular vesicles released from pancreas adenocarcinoma cells (BXPC3) or human breast cancer cells (MCF7) were isolated by differential centrifugation. Human umbilical vein endothelial cells (HUVEC) were cultured for 72  h in the presence or absence of CaCe-dEV. Subsequently, they were washed and re-cultivated over three cycles to get daughter cell generations (DG) which were not exposed to CaCe-dEV. (Source: Thrombosis Research)
Source: Thrombosis Research - September 5, 2019 Category: Hematology Authors: Rania Djedidi-Amrane, Aur élie Rousseau, Annette K. Larsen, Ismail Elalamy, Patrick Van Dreden, Grigoris T. Gerotziafas Tags: Full Length Article Source Type: research

Oral anticoagulant-associated bleeding requiring hospitalization in Thai patients: Incidences, risk factors, and outcomes
Oral anticoagulants have been widely used in patients with atrial fibrillation (AF), mechanical valve and in the treatment of both arterial and venous thromboembolism (VTE). Warfarin is the conventional oral anticoagulant which was established its efficacy and safety profiles. Direct oral anticoagulants (DOACs) including dabigatran, rivaroxaban, and apixaban, have gained their popularity and been increasingly used since their approval in Thailand for stroke prevention in AF and VTE treatment. (Source: Thrombosis Research)
Source: Thrombosis Research - September 5, 2019 Category: Hematology Authors: Kochawan Boonyawat, Patratorn Kunakorntham, Pantep Angchaisuksiri Tags: Letter to the Editors-in-Chief Source Type: research

Ischaemia –reperfusion injury with Pringle's maneuver induces unusually large von Willebrand factor multimers after hepatectomy
This study aimed to clarify the effect of hepatectomy on UL-VWFMs. (Source: Thrombosis Research)
Source: Thrombosis Research - September 5, 2019 Category: Hematology Authors: Takahiro Yoshikawa, Takeo Nomi, Kazuya Sakai, Masaki Hayakawa, Daisuke Hokuto, Yasuko Matsuo, Masayuki Sho, Masanori Matsumoto Tags: Full Length Article Source Type: research

Providers' utilization and perceptions of warfarin dosing algorithms
Warfarin dosing algorithms have proven beneficial in increasing time within therapeutic range (TTR) and decreasing adverse events associated with out-of-range international normalized ratios (INRs). Despite widespread availability, providers' utilization and perceptions of warfarin algorithms in real-world practice are unclear. Identifying perceptions and barriers to algorithm use may help attempts to improve warfarin therapy management. (Source: Thrombosis Research)
Source: Thrombosis Research - September 4, 2019 Category: Hematology Authors: Trevor Gardner, Sara R. Vazquez, Kibum Kim, Aubrey E. Jones, Daniel M. Witt Tags: Full Length Article Source Type: research

Outcomes of direct oral anticoagulant- and warfarin-associated hemorrhage: A single center retrospective cohort study
Direct oral anticoagulants (DOACs) are now a mainstay of anticoagulant therapy for the prevention and treatment of venous thromboembolism (VTE) and stroke prevention in non-valvular atrial fibrillation (a-fib). However, bleeding-related morbidity and mortality remains a feared complication. Bleeding in patients taking DOACs in clinical trials showed comparable, if not more favorable, outcomes compared to that of warfarin [1 –4]. However, the safety of DOAC use should also be evaluated in less selected cohorts in the real-world. (Source: Thrombosis Research)
Source: Thrombosis Research - September 3, 2019 Category: Hematology Authors: Lorraine Cafuir, En Cheng, Christine Kempton Tags: Letter to the Editors-in-Chief Source Type: research

Optimal management of hormonal contraceptives after an episode of venous thromboembolism
Optimal management of hormonal contraception in patients with venous thromboembolism (VTE) requires an individualized approach considering its potential benefits and complications during and after anticoagulant treatment. Potential benefits include prevention of pregnancy and mitigation of menstrual bleeding that is often worsened after start of anticoagulation therapy. Current evidence suggests that patients may opt for a continuation of (all forms of) hormonal contraception during anticoagulant treatment, provided that they are adequately informed by the treating physicians. (Source: Thrombosis Research)
Source: Thrombosis Research - August 31, 2019 Category: Hematology Authors: Frederikus A. Klok, Stefano Barco Tags: Review Article Source Type: research

Fatal pulmonary embolism or bleeding in patients with venous thromboembolism: implications of real-life data
Reliable information on what patients with venous thromboembolism (VTE) are at increased risk to die of pulmonary embolism (PE) or bleeding (and the time course of the two fatal events) could likely help to better use anticoagulant therapy by improving selection of patients in whom its benefit will likely outweigh the risk. Unfortunately, there is scarce information in the literature on the incidence and time-course of fatal PE and fatal bleeding in real life clinical practice. This review article provides an overview of the most important published data on this topic using the RIETE (Registro Informatizado de Enfermedad T...
Source: Thrombosis Research - August 31, 2019 Category: Hematology Authors: Manuel Monreal Tags: Review Article Source Type: research

The role of haemostasis in placenta-mediated complications
Normal pregnancy is associated with an increasing state of activation of the haemostatic system. This activation state is excessive in women with placenta-mediated pregnancy complications (PMPCs), including preeclampsia (PE). Platelet activation plays a crucial pathophysiological role in PE. The very early activation of coagulation in the intervillous space is mandatory for placental growth and morphogenesis but its excesses and/or inadequate control may participate to the emergence of the trophoblastic phenotype of PE. (Source: Thrombosis Research)
Source: Thrombosis Research - August 31, 2019 Category: Hematology Authors: Jean-Christophe Gris, Sylvie Bouvier, Éva Cochery-Nouvellon, Éric Mercier, Ève Mousty, Antonia Pérez-Martin Tags: Full-length Article Source Type: research

Pregnancy after cerebral venous thrombosis
Pregnancy outcome in women with previous cerebral vein thrombosis (CVT) on antithrombotic prophylaxis with low-molecular weight heparin (LMWH) is largely unknown. Aims of this study were to evaluate the risk of recurrent VTE, bleeding and pregnancy outcome in a cohort of pregnant women on LMWH after a first episode of CVT. To estimate the efficacy of LMWH in the prevention of obstetrical complications we compared pregnancies before (without LMWH) with pregnancies after (with LMWH) CVT in 25 women. (Source: Thrombosis Research)
Source: Thrombosis Research - August 31, 2019 Category: Hematology Authors: Maria Abbattista, Marco Capecchi, Ida Martinelli Tags: Full-length Article Source Type: research

DOACS in women: pros and cons
The recent approval of direct-acting oral anticoagulants (DOAC) for long-term anticoagulation in atrial fibrillation and venous thromboembolism resulted in a rapid implementation of these new drugs into daily care. Although DOAC dosing is similar for women and men and, overall, results in comparable outcomes, sex specific issues need to be considered. This review will discuss DOAC topics specifically related to women's health, including the risks and benefits of DOAC treatment for women, the issue of abnormal uterine bleeding from DOAC and the risk and management of DOAC exposure in pregnancy. (Source: Thrombosis Research)
Source: Thrombosis Research - August 31, 2019 Category: Hematology Authors: Jan Beyer-Westendorf Tags: Full-length Article Source Type: research