A multi-centre evaluation of TECHNOSCREEN ® ADAMTS-13 Activity assay as a screening tool for detecting deficiency of ADAMTS-13.
CONCLUSIONS: The screening test provides more rapid ADAMTS-13 level evaluation than most currently available assays. Its simple operation renders it suitable for adoption in routine or specialist laboratory environments. PMID: 32239643 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - April 2, 2020 Category: Hematology Authors: Moore GW, Meijer D, Griffiths M, Rushen L, Brown A, Budde U, Dittmer R, Schocke B, Leyte A, Geiter S, Moes A, Cutler JA, Binder NB Tags: J Thromb Haemost Source Type: research

The versatile heparin in COVID-19.
Abstract Coagulopathy in corona virus infection has been shown to be associated with high mortality with high D-dimers being a particularly important marker for the coagulopathy.1 In the latest paper from the same group, the use of anticoagulant therapy with heparin was shown to decrease mortality as well. 2 This is especially so in patients i) who have met the sepsis induced coagulopathy (SIC) criteria ≥4 (40.0% vs 64.2%, P=0.029) compared to those with SIC score
Source: Thrombosis and Haemostasis - April 2, 2020 Category: Hematology Authors: Thachil J Tags: J Thromb Haemost Source Type: research

Activated platelets kill Staphylococcus aureus, but not Streptococcus pneumoniae - The role of Fc γRIIa and platelet factor 4/heparin-antibodies.
CONCLUSIONS: Anti-PF4/H antibodies seem to have only a minor role for direct killing of Gram-positive bacteria by platelets. S.aureus is killed by platelets or platelet releasate. In contrast, S.pneumoniae affect platelet viability. PMID: 32237268 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - April 1, 2020 Category: Hematology Authors: Wolff M, Handtke S, Palankar R, Wesche J, Kohler TP, Kohler C, Gruel Y, Hammerschmidt S, Greinacher A Tags: J Thromb Haemost Source Type: research

Efficacy and safety of recombinant human soluble thrombomodulin in patients with sepsis-associated coagulopathy: a systematic review and meta-analysis.
CONCLUSIONS: In patients with sepsis, SAC is associated with higher 28-day mortality. The administration of rhsTM reduced 28-day mortality in patients with SAC, but not in those without SAC. PMID: 32237269 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - April 1, 2020 Category: Hematology Authors: Valeriani E, Squizzato A, Gallo A, Porreca E, Vincent JL, Iba T, Hagiwara A, Di Nisio M Tags: J Thromb Haemost Source Type: research

Considerations when choosing an appropriate bleeding risk assessment tool for patients with atrial fibrillation.
PMID: 32233026 [PubMed - in process] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - April 1, 2020 Category: Hematology Authors: Ding WY, Harrison SL, Lane DA, Lip GYH Tags: J Thromb Haemost Source Type: research

Crosstalk between hemostasis and lymphangiogenesis.
PMID: 32233027 [PubMed - in process] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - April 1, 2020 Category: Hematology Authors: Suzuki-Inoue K, Tsukiji N, Otake S Tags: J Thromb Haemost Source Type: research

What the neighbors say.
Authors: PMID: 32233028 [PubMed - in process] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - April 1, 2020 Category: Hematology Tags: J Thromb Haemost Source Type: research

Antisense Oligonucleotide Targeting of Thrombopoietin Represents A Novel Platelet Depletion Method to Assess the Immunomodulatory Role of Platelets.
CONCLUSIONS: Herein, we describe how in mice, ASO-mediated silencing of hepatic TPO expression reduces platelet, megakaryocyte, and megakaryocyte progenitor count, without altering platelet activity. TPO ASO-mediated platelet depletion can be achieved acutely and sustained chronically in the absence of adverse bleeding. TPO ASO-mediated platelet depletion allows for the reintroduction of new platelets, an advantage over commonly used antibody-mediated depletion strategies. Using a murine model of lung inflammation, we demonstrate that platelet depletion, induced by either TPO ASO or anti-CD42b treatment, reduces the accumu...
Source: Thrombosis and Haemostasis - March 30, 2020 Category: Hematology Authors: Barrett TJ, Wu BG, Revenko AS, MacLeod AR, Segal LN, Berger JS Tags: J Thromb Haemost Source Type: research

Anticoagulant treatment is associated with decreased mortality in severe coronavirus disease 2019 patients with coagulopathy.
CONCLUSIONS: Anticoagulant therapy mainly with LMWH appears to be associated with better prognosis in severe COVID-19 patients meeting SIC criteria or with markedly elevated D-dimer. PMID: 32220112 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 27, 2020 Category: Hematology Authors: Tang N, Bai H, Chen X, Gong J, Li D, Sun Z Tags: J Thromb Haemost Source Type: research

Management of Major Bleeds in Patients with Immune Thrombocytopenia.
Abstract BACKGROUND: A standard approach to the recognition and management of major bleeding in immune thrombocytopenia (ITP) is lacking. METHODS: Retrospective cohort study of ITP patients presenting to the ED with severe thrombocytopenia (platelet count
Source: Thrombosis and Haemostasis - March 26, 2020 Category: Hematology Authors: Mithoowani S, Cervi A, Shah N, Ejaz R, Sirotich E, Barty R, Li N, Nazy I, Arnold DM Tags: J Thromb Haemost Source Type: research

Anti-Factor IIa (FIIa) Heparin Assay for Patients on Direct Factor Xa (FXa) Inhibitors.
CONCLUSIONS: The BIOPHEN™ ANTI-IIa assay is suitable for patients transitioning off apixaban or rivaroxaban. PMID: 32216028 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 25, 2020 Category: Hematology Authors: Stuart M, Johnson L, Hanigan S, Pipe SW, Li SH Tags: J Thromb Haemost Source Type: research

Disseminated intravascular coagulation in patients with 2019-nCoV pneumonia.
PMID: 32212240 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 24, 2020 Category: Hematology Authors: Lillicrap D Tags: J Thromb Haemost Source Type: research

Severe Plasma Prekallikrein Deficiency: Clinical Characteristics, Novel KLKB1 Mutations, and Estimated Prevalence.
CONCLUSIONS: We characterized the genetic background of severe PK deficiency, critically appraised mutations, and provided prevalence estimates. Our data on laboratory characteristics and clinical course of severe PK deficiency may have clinical implications. PMID: 32202057 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 23, 2020 Category: Hematology Authors: Barco S, Sollfrank S, Trinchero A, Adenaeuer A, Abolghasemi H, Conti L, Häuser F, Kremer Hovinga JA, Lackner KJ, Loewecke F, Miloni E, Vazifeh Shiran N, Tomao L, Wuillemin WA, Zieger B, Lämmle B, Rossmann H Tags: J Thromb Haemost Source Type: research

Impact of concomitant antiplatelet therapy on the efficacy and safety of DOACs for acute venous thromboembolism: systematic review and meta-analysis.
CONCLUSIONS: Concomitant use of antiplatelet therapy with oral anticoagulants does not appear to affect the risk of recurrent VTE and increases the risk of major bleeding. PMID: 32202042 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 22, 2020 Category: Hematology Authors: Valeriani E, Porreca E, Weitz JI, Schulman S, Candeloro M, Di Nisio M Tags: J Thromb Haemost Source Type: research

Fibrin exposure triggers αIIbβ3-independent platelet aggregate formation, ADAM10 activity and glycoprotein VI shedding in a charge-dependent manner.
Fibrin exposure triggers αIIbβ3-independent platelet aggregate formation, ADAM10 activity and glycoprotein VI shedding in a charge-dependent manner. J Thromb Haemost. 2020 Mar 21;: Authors: Montague SJ, Hicks SM, Lee CS, Coupland LA, Parish CR, Lee WM, Andrews RK, Gardiner EE Abstract Collagen and fibrin engagement and activation of glycoprotein (GP) VI induces proteolytic cleavage of the GPVI ectodomain generating shed soluble GPVI (sGPVI). Collagen-mediated GPVI shedding requires intracellular signalling to release the sGPVI, mediated by A Disintegrin And Metalloproteinase 10 (ADAM10), ho...
Source: Thrombosis and Haemostasis - March 21, 2020 Category: Hematology Authors: Montague SJ, Hicks SM, Lee CS, Coupland LA, Parish CR, Lee WM, Andrews RK, Gardiner EE Tags: J Thromb Haemost Source Type: research

FVIII activity following FVIII protein infusion or FVIII gene transfer predicts the bleeding risk in hemophilia A rats.
CONCLUSIONS: after 12 weeks of observation, both approaches significantly reduced bleeds per animal and increased the proportion of bleed-free animals compared to controls (43% vs. 0%, respectively [AAV]; 75% vs. 8%, respectively [injection]). Both approaches resulted in an anti-FVIII inhibitory response in 20-37% of treated animals, similar to HA patients. Inhibitory antibodies were refractory to clinical improvement (reduction of bleeds) only in the AAV-based prophylaxis. An integrated model-based analysis of data on FVIII exposure and bleeding events was performed. This predicted the bleeding risk at any given circulati...
Source: Thrombosis and Haemostasis - March 20, 2020 Category: Hematology Authors: Lövgren KM, Larsen MS, Zintner SM, Small JC, Kjelgaard-Hansen M, Häger M, Petersen M, Wiinberg B, Margaritis P Tags: J Thromb Haemost Source Type: research

Coagulation status of critically ill patients with and without liver disease assessed using a novel thrombin generation analyser.
Abstract The liver synthesises the majority of pro- and anti- coagulant and fibrinolytic proteins, and during liver dysfunction synthesis of these proteins is reduced. The end point of conventional haemostatic tests, such as the prothrombin time (PT), occurs when only 5% of thrombin generation (TG) has taken place and is not sensitive to the effects of natural anti-coagulants. The aim of this study was to determine whether TG in the presence of thrombomodulin (TM) provides more useful information about coagulation potential, in comparison to the PT. Analysis was performed on ST Genesia, a novel TG analyser from Di...
Source: Thrombosis and Haemostasis - March 20, 2020 Category: Hematology Authors: Morrow GB, Beavis J, Harper S, Baker P, Desbourough MJR, Curry N, Stanworth SJ, Laffan MA Tags: J Thromb Haemost Source Type: research

Long-Term Safety and Efficacy of Recombinant Coagulation Factor IX Albumin Fusion Protein (rIX-FP) in Previously Treated Pediatric Patients with Hemophilia B: Results from a Phase 3b Extension Study.
CONCLUSION:  This extension study demonstrated the long-term safety and efficacy of weekly rIX-FP in pediatric patients. Additionally, it showed that adequate bleed protection can be achieved with 10- or 14-day rIX-FP regimens in selected pediatric patients while maintaining safety. PMID: 32185782 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 17, 2020 Category: Hematology Authors: Kenet G, Chambost H, Male C, Halimeh S, Lambert T, Li Y, Seifert W, Santagostino E Tags: Thromb Haemost Source Type: research

Antiphosphatidylserine/Prothrombin Antibodies: An Update on Their Association with Clinical Manifestations of Antiphospholipid Syndrome.
Abstract OBJECTIVE:  The aim of the study is to perform a systematic review on the recent available evidence on antiphosphatidylserine/prothrombin (aPS/PT) antibodies and their association with clinical manifestations of the antiphospholipid syndrome (APS). METHODS:  A detailed literature search was applied a priori to Ovid MEDLINE, In-Process and Other Non-Indexed Citation 2012 to present and to abstract from EULAR and ACR/ARHP Annual Meetings (2012-2019). RESULTS:  Data from 2,901 patients, 587 diseases controls and 559 healthy controls included in 15 retrieved studies was analyzed. The ...
Source: Thrombosis and Haemostasis - March 17, 2020 Category: Hematology Authors: Radin M, Foddai SG, Cecchi I, Rubini E, Schreiber K, Roccatello D, Bertolaccini ML, Sciascia S Tags: Thromb Haemost Source Type: research

Antiphospholipid Syndrome and the Relationship between Laboratory Assay Positivity and prevalence of Non-Bacterial Thrombotic Endocarditis: A Retrospective Cohort Study.
Abstract BACKGROUND: Non-bacterial thrombotic endocarditis (NBTE) is a potential complication of antiphospholipid syndrome (APS) manifesting as non-infectious lesions on one or more cardiac valves. There are limited tools to inform clinicians regarding which APS patients would benefit most from echocardiographic screening for this complication. OBJECTIVES: We tested the hypothesis that the risk of both prevalent and incident NBTE is directly related to the number of positive laboratory assays for APS. PATIENTS/METHODS: In this single-center retrospective cohort study design, consecutive patients with con...
Source: Thrombosis and Haemostasis - March 17, 2020 Category: Hematology Authors: Lenz CJ, Mankad R, Klarich K, Kurmann R, McBane RD Tags: J Thromb Haemost Source Type: research

The impact of prepartum factor XIII activity on postpartum blood loss.
Abstract BACKGROUND: Postpartum hemorrhage (PPH), a major cause of maternal mortality, has several known risk factors but frequently occurs unexpectedly. PPH incidence and related maternal morbidity and mortality are rising worldwide. OBJECTIVE: To evaluate the impact of defined prepartum blood coagulation parameters on postpartum blood loss. METHODS: This single-center, prospective cohort study analyzed prepartum activities of coagulation factors II and XIII and fibrinogen levels in 1300 women. Blood samples were obtained at labor onset and analyzed only after the last patient had delivered, ensuring to...
Source: Thrombosis and Haemostasis - March 16, 2020 Category: Hematology Authors: Haslinger C, Korte W, Hothorn T, Brun R, Greenberg C, Zimmermann R Tags: J Thromb Haemost Source Type: research

Switching from vitamin K antagonists to direct oral anticoagulants: Treatment satisfaction and patient concerns.
CONCLUSIONS: DOAC treatment satisfaction was high. A substantial part of patients expressed concerns about adverse events, especially women, patients aged
Source: Thrombosis and Haemostasis - March 14, 2020 Category: Hematology Authors: Toorop MMA, van Rein N, Nierman MC, Vermaas HW, Huisman MV, van der Meer FJM, Cannegieter SC, Lijfering WM Tags: J Thromb Haemost Source Type: research

Combination of two complementary automated rapid assays for diagnosis of heparin-induced thrombocytopenia (HIT).
Abstract BACKGROUND: HIT diagnosis typically utilizes complementary diagnostic assays, e.g., a PF4-dependent enzyme-immunoassay (EIA) and a platelet activation assay such as the serotonin-release assay (SRA). OBJECTIVES: To determine whether the combination of two automated assays-a latex immunoturbidimetric assay (LIA) that evaluates competitive inhibition of a HIT-like monoclonal antibody and a chemiluminescence immunoassay (CLIA) for detecting anti-PF4/heparin IgG-optimizes diagnostic sensitivity while also yielding good specificity, particularly at high assay reactivities. PATIENTS/METHODS: We determ...
Source: Thrombosis and Haemostasis - March 13, 2020 Category: Hematology Authors: Warkentin TE, Sheppard JI, Smith JW, Na L, Moore JC, Arnold DM, Nazy I Tags: J Thromb Haemost Source Type: research

Validation of risk assessment models predicting venous thromboembolism in acutely ill medical inpatients: a cohort study.
CONCLUSION: In our study, Caprini, IMPROVE and Padua VTE risk scores have poor discriminative ability to identify not-critically ill medical inpatients at risk of VTE, and do not perform better than a risk evaluation based on patient's age alone. PMID: 32168402 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 13, 2020 Category: Hematology Authors: Moumneh T, Riou J, Douillet D, Henni S, Mottier D, Tritschler T, Le Gal G, Roy PM Tags: J Thromb Haemost Source Type: research

N-glycosylation of blood coagulation factor XIII subunit B and its functional consequence.
CONCLUSION: Characterization of the glycan moieties attached to FXIII-B is reported for the first time. Complete deglycosylation of the native protein was achieved by a deglycosylation workflow. The associated glycan structure is not required for FXIII-B dimer formation, but it very likely prolongs the half-life of FXIII-B in the plasma. PMID: 32168410 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 13, 2020 Category: Hematology Authors: Hurják B, Kovács Z, Döncző B, Katona É, Haramura G, Erdélyi F, Shemirani AH, Sadeghi F, Muszbek L, Guttman A Tags: J Thromb Haemost Source Type: research

External validation of the SOX-PTS score in a prospective multicenter trial of patients with proximal deep vein thrombosis.
CONCLUSION: We externally validated the SOX-PTS score for estimating the risk of developing PTS, moderate to severe PTS, and severe PTS, in patients with proximal DVT. The score may be useful to predict PTS at the time of DVT diagnosis. Further external validation in different patient cohorts is required. PMID: 32145144 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 7, 2020 Category: Hematology Authors: Rabinovich A, Gu CS, Vedantham S, Kearon C, Goldhaber SZ, L Gornik H, Kahn SR, ATTRACT Tags: J Thromb Haemost Source Type: research

Annexin A4 inhibits sulfatide-induced activation of coagulation factor XII.
CONCLUSIONS: We found that ANXA3, ANXA4, and ANXA5 strongly inhibited sulfatide-induced plasma coagulation. Wild-type and mutated ANXA4 were used to clarify the molecular mechanism involved in inhibition. ANXA4 inhibited sulfatide-induced auto-activation of FXII to FXIIa and the conversion of its natural substrate FXI to FXIa but showed no effect on the protease activity of FXIIa or FXIa. Alanine scanning showed that substitution of the Ca2+ -binding amino acid residue in the fourth fold of the core domain of ANXA4 reduced anticoagulant activity, and deletion of the entire fourth fold of the core domain resulted in complet...
Source: Thrombosis and Haemostasis - March 7, 2020 Category: Hematology Authors: Nakayama M, Miyagawa H, Kuranami Y, Tsunooka-Ota M, Yamaguchi Y, Kojima-Aikawa K Tags: J Thromb Haemost Source Type: research

Coagulation factor VIIa binds to herpes simplex virus 1-encoded glycoprotein C forming a factor X-enhanced tenase complex oriented on membranes.
CONCLUSIONS: The assembly of gC with FVIIa/FX parallels that of TF and may involve other constituents on the HSV1 envelope with implications in virus infection and pathology. PMID: 32145149 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 7, 2020 Category: Hematology Authors: Lin BH, Sutherland MR, Rosell FI, Morrissey JH, Pryzdial ELG Tags: J Thromb Haemost Source Type: research

Thrombin Promotes Macrophage Polarization into M1-Like Phenotype to Induce Inflammatory Responses.
In this study, we investigated changes in gene expression of selected cytokines and chemokines and their secretion profiles following thrombin stimulation of murine macrophages. Thrombin promoted differentiation of macrophages into an M1-like phenotype that was associated with the expression of classical pro-inflammatory markers. The cellular actions of thrombin on macrophages were mediated in part by protease-activated receptor-1 (PAR-1) and were dependent on phosphoinositide 3-kinase/AKT and nuclear factor-κB. Moreover, heat-denatured thrombin stimulated the secretion of some pro-inflammatory mediators to the same ...
Source: Thrombosis and Haemostasis - March 4, 2020 Category: Hematology Authors: López-Zambrano M, Rodriguez-Montesinos J, Crespo-Avilan GE, Muñoz-Vega M, Preissner KT Tags: Thromb Haemost Source Type: research

Pretransplant Genetic Susceptibility: Clinical Relevance in Transplant-Associated Thrombotic Microangiopathy.
In conclusion, increased incidence of pathogenic, rare and variants in exonic/splicing/UTR regions of TA-TMA patients suggests genetic susceptibility not evident in controls or donors. Notably, variants in exonic/splicing/UTR regions were associated with poor response and survival. Therefore, pretransplant genomic screening may be useful to intensify monitoring and early intervention in patients at high risk for TA-TMA. PMID: 32131130 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 4, 2020 Category: Hematology Authors: Gavriilaki E, Touloumenidou T, Sakellari I, Batsis I, Mallouri D, Psomopoulos F, Tsagiopoulou M, Koutra M, Yannaki E, Papalexandri A, Taylor P, Nikolousis E, Stamouli M, Holbro A, Baltadakis I, Liga M, Spyridonidis A, Tsirigotis P, Charchalakis N, Tsakiri Tags: Thromb Haemost Source Type: research

Prognosis in patients with cancer-associated venous thromboembolism: Comparison of the RIETE-VTE and modified Ottawa score.
CONCLUSIONS: Both the RIETE-VTE and modified Ottawa score accurately identified elderly patients with CAT who are at low-risk for short-term mortality and who are potential candidates for outpatient care. PMID: 32124545 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 2, 2020 Category: Hematology Authors: Pfaundler N, Limacher A, Stalder O, Méan M, Rodondi N, Baumgartner C, Aujesky D Tags: J Thromb Haemost Source Type: research

Response to: Direct oral anticoagulants: Still too early for prime time after pulmonary endarterectomy?
PMID: 32112527 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 1, 2020 Category: Hematology Authors: Bunclark K, Toshner M Tags: J Thromb Haemost Source Type: research

Reply to "Comment on 'Comparison of three transfusion protocols prior to central venous catheterization in patients with cirrhosis; a randomized controlled trial'".
Reply to "Comment on 'Comparison of three transfusion protocols prior to central venous catheterization in patients with cirrhosis; a randomized controlled trial'". J Thromb Haemost. 2020 Mar;18(3):754-755 Authors: Rocha LL, Corrêa TD PMID: 32112528 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 1, 2020 Category: Hematology Authors: Rocha LL, Corrêa TD Tags: J Thromb Haemost Source Type: research

Comment on Comparison of three transfusion protocols prior to central venous catheterisation in patients with cirrhosis; a randomised controlled trial.
PMID: 32112529 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 1, 2020 Category: Hematology Authors: Roberts LN, Arya R, Hogan BJ Tags: J Thromb Haemost Source Type: research

Response to letter to the Editor: Direct oral anticoagulants in thrombotic antiphospholipid syndrome associated with chronic thromboembolic pulmonary hypertension.
PMID: 32112530 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 1, 2020 Category: Hematology Authors: Bunclark K, Ansaripour A, Sheares K, Toshner M Tags: J Thromb Haemost Source Type: research

What the neighbors say.
Authors: PMID: 32112531 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 1, 2020 Category: Hematology Tags: J Thromb Haemost Source Type: research

Antithrombin: An anticoagulant, anti-inflammatory and antibacterial serpin.
PMID: 32112532 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 1, 2020 Category: Hematology Authors: Rezaie AR, Giri H Tags: J Thromb Haemost Source Type: research

Defining trauma-induced coagulopathy with respect to future implications for patient management: Communication from the SSC of the ISTH.
inolysis, Disseminated Intravascular Coagulation, and Perioperative and Critical Care Thrombosis and Hemostasis PMID: 32112533 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 1, 2020 Category: Hematology Authors: Moore HB, Gando S, Iba T, Kim PY, Yeh CH, Brohi K, Hunt BJ, Levy JH, Draxler DF, Stanworth S, Görlinger K, Neal MD, Schreiber MA, Barrett CD, Medcalf RL, Moore EE, Mutch NJ, Thachil J, Urano T, Thomas S, Scărlătescu E, Walsh M, Subcommittees on Fibrino Tags: J Thromb Haemost Source Type: research

Direct oral anticoagulants: Still too early for prime time after pulmonary endarteriectomy?
e X PMID: 32112534 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 1, 2020 Category: Hematology Authors: Bertoletti L, Jaïs X, Bezzeghoud S, Delavenne X Tags: J Thromb Haemost Source Type: research

In vitro flow based systems to study platelet function and thrombus formation: Recommendations for standardization: Communication from the SSC on Biorheology of the ISTH.
Abstract Experimental videomicroscopic in vitro assays of thrombus formation based on blood perfusion are instrumental in a wide range of basic studies in thrombosis, screening for hereditary or acquired plateletrelated pathologies, and assessing the effectiveness of novel anti-platelet therapies. Here, we discuss application of the broadly used "in vitro thrombosis model": a frequently used assay to study the formation of 3D aggregates under flow, which involves perfusing anticoagulated whole blood over fibrillar collagen in a flow geometry of rectangular cross-section, such as glass microcapillaries or...
Source: Thrombosis and Haemostasis - March 1, 2020 Category: Hematology Authors: Mangin PH, Gardiner EE, Nesbitt WS, Kerrigan SW, Korin N, Lam WA, Panteleev MA, Subcommittee on Biorheology Tags: J Thromb Haemost Source Type: research

Commentary on: Comparison of three transfusion protocols prior to central venous catheterization in patients with cirrhosis: A randomized controlled trial.
PMID: 32112536 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 1, 2020 Category: Hematology Authors: Intagliata NM, Villa E Tags: J Thromb Haemost Source Type: research

Direct oral anticoagulants in thrombotic antiphospholipid syndrome associated with chronic thromboembolic pulmonary hypertension.
PMID: 32112537 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - March 1, 2020 Category: Hematology Authors: Porres-Aguilar M, Kahn SR, Hirsch AM Tags: J Thromb Haemost Source Type: research

An international collaborative study to establish the WHO 4th International Standard for Streptokinase: communication from the SSC of the ISTH.
Abstract Streptokinase is used worldwide as a cost-effective treatment for acute myocardial infarction (AMI). Manufacturers use the WHO International Standard (IS) for Streptokinase to potency label their products, ensuring consistent, safe, and effective dosing. Stocks of the 3rd IS for Streptokinase (coded 00/464) are running low, and an international collaborative study was organised to calibrate a replacement. A total of 15 laboratories from 9 countries took part, using chromogenic and/or fibrin clot lysis methods to determine the potency of two candidate preparations, coded 16/356 (sample B) and 16/358 (sampl...
Source: Thrombosis and Haemostasis - February 29, 2020 Category: Hematology Authors: Locke M, Rigsby P, Longstaff C, Fibrinolysis subcommittee Tags: J Thromb Haemost Source Type: research

A novel assay for studying the involvement of blood cells in whole blood thrombin generation.
CONCLUSIONS: A novel WB-TG assay was developed, which is a straight forward tool to measure the involvement of platelets and erythrocytes in TG and may assist the research of blood cells-associated coagulation disorders. PMID: 32108990 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - February 28, 2020 Category: Hematology Authors: Wan J, Konings J, Yan Q, Kelchtermans H, Kremers R, de Laat B, Roest M Tags: J Thromb Haemost Source Type: research

Investigating the clearance of VWF A-domains using site-directed PEGylation and novel N-linked glycosylation.
CONCLUSIONS: These novel data demonstrate that site specific PEGylation but not site specific N-glycosylation modifies LRP1-dependent uptake of the A1A2A3-VWF by macrophages. This suggests that PEGylation, within the A1- and A3-domains in particular, may be used to attenuate LRP1-mediated clearance of VWF. PMID: 32108991 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - February 28, 2020 Category: Hematology Authors: Fazavana J, Brophy TM, Chion A, Cooke N, Terraube V, Cohen J, Parng C, Pittman D, Cunningham O, Lambert M, O'Donnell JS, O'Sullivan JM Tags: J Thromb Haemost Source Type: research

Large and small platelets - (when) do they differ?
Abstract Platelets are most important to provide cellular hemostasis but also take part in inflammation and immune processes. Increased platelet size has been regarded as a feature describing a young and more reactive subpopulation until studies were published which questioned this concept. Changes of platelet size given by the mean platelet volume (MPV) were described for immune thrombocytopenia, cardiovascular disease, atherosclerosis, venous thromboembolism, chronic lung disease, sepsis, cancer associated thrombosis, autoimmune disorders and others. This review summarizes the literature on what is known about p...
Source: Thrombosis and Haemostasis - February 28, 2020 Category: Hematology Authors: Handtke S, Thiele T Tags: J Thromb Haemost Source Type: research

Contribution of exome sequencing to the identification of genes involved in the response to clopidogrel in cardiovascular patients.
Abstract BACKGROUND: On-clopidogrel platelet reactivity (PR) is associated with the risk of thrombotic or bleeding event in selected populations of high-risk patients. PR is a highly heritable phenotype and a few variants of cytochrome genes, essentially CYP2C19, are associated with PR but only explain 5-12% of the variability. OBJECTIVE: The aim of this study is to delineate genetic determinants of on-clopidogrel PR using high throughput sequencing. METHODS: We performed a whole exome sequencing of 96 low- and matched high-PR patients in a discovery cohort. Exomes from genes with variants significantly ...
Source: Thrombosis and Haemostasis - February 20, 2020 Category: Hematology Authors: Fontana P, Ibberson M, Stevenson B, Wigger L, Daali Y, Niknejad A, Mach F, Docquier M, Xenarios I, Cuisset T, Alessi MC, Reny JL Tags: J Thromb Haemost Source Type: research

Gly197Arg mutation in protein C causes recurrent thrombosis in a heterozygous carrier.
CONCLUSION: The G197R mutation in patients appears to be functionally equivalent to a heterozygous protein C knockout with half of the protein having no significant activity and thus causing thrombosis. PMID: 32078247 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - February 20, 2020 Category: Hematology Authors: Lu Y, Giri H, Villoutreix BO, Ding Q, Wang X, Rezaie AR Tags: J Thromb Haemost Source Type: research

Identification and functional characterization of a novel splicing variant in the F8 coagulation gene causing severe hemophilia A.
CONCLUSIONS: Herein, we demonstrate the aberrant impact of a FVIII synonymous mutation on its transcription, activity and pathological outcomes. Our data underline the importance of increasing the knowledge regarding the functional consequences of F8 mutations and their link to inhibitor development and an effective replacement therapy. PMID: 32078252 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - February 20, 2020 Category: Hematology Authors: Famà R, Borroni E, Zanolini D, Merlin S, Bruscaggin V, Walker GE, Olgasi C, Babu D, Agnelli Giacchello J, Valeri F, Giordano M, Borchiellini A, Follenzi A Tags: J Thromb Haemost Source Type: research

Long-term safety and efficacy of rIX-FP prophylaxis with extended dosing intervals up to 21 days in adults/adolescents with hemophilia B.
CONCLUSIONS: rIX-FP extended interval prophylaxis provides dosing flexibility and, in selected patients, a 21-day regimen may provide an alternative option to minimize treatment burden and individualize treatment. PMID: 32078256 [PubMed - as supplied by publisher] (Source: Thrombosis and Haemostasis)
Source: Thrombosis and Haemostasis - February 20, 2020 Category: Hematology Authors: Mancuso ME, Lubetsky A, Pan-Petesch B, Lissitchkov T, Nagao A, Seifert W, Li Y, Santagostino E Tags: J Thromb Haemost Source Type: research