GLYR1 transcriptionally regulates PER3 expression to promote the proliferation and migration of multiple myeloma
Genomics. 2024 Apr 18:110846. doi: 10.1016/j.ygeno.2024.110846. Online ahead of print.ABSTRACTPeriod circadian regulator 3 (PER3) functions as a tumor suppressor in various cancers. However, the role of PER3 in multiple myeloma (MM) has not been reported yet. Through this study, we aimed to investigate the potential role of PER3 in MM and the underlying mechanisms. RT-qPCR and western blotting were used to determine the mRNA and protein expression levels of PER3. Glyoxylate reductase 1 homolog (GLYR1) was predicted to be a transcription factor of PER3. The binding sites of GLYR1 on the promoter region of PER3 were analyzed...
Source: Genomics - April 20, 2024 Category: Genetics & Stem Cells Authors: Xiao Yan Kaihong Xu Zhijuan Xu Cong Shi Binbin Lai Hao Wu Shujun Yang Lixia Sheng Keting Wang Yuhan Zheng Guifang Ouyang Di Yang Source Type: research
GLYR1 transcriptionally regulates PER3 expression to promote the proliferation and migration of multiple myeloma
Genomics. 2024 Apr 18:110846. doi: 10.1016/j.ygeno.2024.110846. Online ahead of print.ABSTRACTPeriod circadian regulator 3 (PER3) functions as a tumor suppressor in various cancers. However, the role of PER3 in multiple myeloma (MM) has not been reported yet. Through this study, we aimed to investigate the potential role of PER3 in MM and the underlying mechanisms. RT-qPCR and western blotting were used to determine the mRNA and protein expression levels of PER3. Glyoxylate reductase 1 homolog (GLYR1) was predicted to be a transcription factor of PER3. The binding sites of GLYR1 on the promoter region of PER3 were analyzed...
Source: Genomics - April 20, 2024 Category: Genetics & Stem Cells Authors: Xiao Yan Kaihong Xu Zhijuan Xu Cong Shi Binbin Lai Hao Wu Shujun Yang Lixia Sheng Keting Wang Yuhan Zheng Guifang Ouyang Di Yang Source Type: research
Comparative genomics on chloroplasts of Rubus (Rosaceae)
In this study, we sequenced, assembled, and characterized 17 plastomes of Rubus, and conducted comparative genomics integrating with 47 previously issued plastomes of this genus. The 64 plastomes of Rubus exhibited typical quadripartite structure with sizes ranging from 155,144 to 156,700 bp, and contained 132 genes including 87 protein-coding genes, 37 tRNA genes and eight rRNA genes. All plastomes are conservative in the gene order, the frequency of different types of long repeats and simple sequence repeats (SSRs), the codon usage, and the selection pressure of protein-coding genes. However, there are also some differen...
Source: Genomics - April 13, 2024 Category: Genetics & Stem Cells Authors: Qing Lu Qin Tian Wei Gu Chen-Xuan Yang Ding-Jie Wang Ting-Shuang Yi Source Type: research
Comparative genomics on chloroplasts of Rubus (Rosaceae)
In this study, we sequenced, assembled, and characterized 17 plastomes of Rubus, and conducted comparative genomics integrating with 47 previously issued plastomes of this genus. The 64 plastomes of Rubus exhibited typical quadripartite structure with sizes ranging from 155,144 to 156,700 bp, and contained 132 genes including 87 protein-coding genes, 37 tRNA genes and eight rRNA genes. All plastomes are conservative in the gene order, the frequency of different types of long repeats and simple sequence repeats (SSRs), the codon usage, and the selection pressure of protein-coding genes. However, there are also some differen...
Source: Genomics - April 13, 2024 Category: Genetics & Stem Cells Authors: Qing Lu Qin Tian Wei Gu Chen-Xuan Yang Ding-Jie Wang Ting-Shuang Yi Source Type: research
Comparative genomics on chloroplasts of Rubus (Rosaceae)
In this study, we sequenced, assembled, and characterized 17 plastomes of Rubus, and conducted comparative genomics integrating with 47 previously issued plastomes of this genus. The 64 plastomes of Rubus exhibited typical quadripartite structure with sizes ranging from 155,144 to 156,700 bp, and contained 132 genes including 87 protein-coding genes, 37 tRNA genes and eight rRNA genes. All plastomes are conservative in the gene order, the frequency of different types of long repeats and simple sequence repeats (SSRs), the codon usage, and the selection pressure of protein-coding genes. However, there are also some differen...
Source: Genomics - April 13, 2024 Category: Genetics & Stem Cells Authors: Qing Lu Qin Tian Wei Gu Chen-Xuan Yang Ding-Jie Wang Ting-Shuang Yi Source Type: research
Comparative genomics on chloroplasts of Rubus (Rosaceae)
In this study, we sequenced, assembled, and characterized 17 plastomes of Rubus, and conducted comparative genomics integrating with 47 previously issued plastomes of this genus. The 64 plastomes of Rubus exhibited typical quadripartite structure with sizes ranging from 155,144 to 156,700 bp, and contained 132 genes including 87 protein-coding genes, 37 tRNA genes and eight rRNA genes. All plastomes are conservative in the gene order, the frequency of different types of long repeats and simple sequence repeats (SSRs), the codon usage, and the selection pressure of protein-coding genes. However, there are also some differen...
Source: Genomics - April 13, 2024 Category: Genetics & Stem Cells Authors: Qing Lu Qin Tian Wei Gu Chen-Xuan Yang Ding-Jie Wang Ting-Shuang Yi Source Type: research
Genetic structural analysis of different breeds and geographical groups of Fenneropenaeus chinensis reveals population diversity
This study investigated eight F. chinensis populations in China, including four geographical populations, three commercial breeds, and one wild population captured from the Yellow Sea. Population stratification analysis revealed that the Hebei geographical population and commercial breeding "Huanghai No. 4" were relatively independent and stable, reflecting a relatively closed breeding environment, whereas gene introgression was present between other populations. Selective signature analysis detected artificial selection for vision, growth, and disease resistance in the Hebei population. Neuronal development-related genes ...
Source: Genomics - April 12, 2024 Category: Genetics & Stem Cells Authors: Qiong Wang Yuhan Jiang Jian Li Jitao Li Yuying He Source Type: research
Melatonin promotes proliferation of Inner Mongolia cashmere goat hair follicle papilla cells through Wnt10b
Genomics. 2024 Apr 10:110844. doi: 10.1016/j.ygeno.2024.110844. Online ahead of print.ABSTRACTThe study demonstrated that melatonin (MT) can induce the development of secondary hair follicles in Inner Mongolian cashmere goats through the Wnt10b gene, leading to secondary dehairing. However, the mechanisms underlying the expression and molecular function of Wnt10b in dermal papilla cells (DPC) remain unknown. This research aimed to investigate the impact of MT on DPC and the regulation of Wnt10b expression, function, and molecular mechanisms in DPC. The findings revealed that MT promotes DPC proliferation and enhances DPC a...
Source: Genomics - April 12, 2024 Category: Genetics & Stem Cells Authors: Wendian Gong Junyang Liu Qing Mu Tergel Chahaer Jiasen Liu Wenqi Ding Tugeqin Bou Zixian Wu Yanhong Zhao Source Type: research
Advancements in long-read genome sequencing technologies and algorithms
Genomics. 2024 Apr 10:110842. doi: 10.1016/j.ygeno.2024.110842. Online ahead of print.ABSTRACTThe recent advent of long read sequencing technologies, such as Pacific Biosciences (PacBio) and Oxford Nanopore technology (ONT), have led to substantial improvements in accuracy and computational cost in sequencing genomes. However, de novo whole-genome assembly still presents significant challenges related to the quality of the results. Pursuing de novo whole-genome assembly remains a formidable challenge, underscored by intricate considerations surrounding computational demands and result quality. As sequencing accuracy and th...
Source: Genomics - April 12, 2024 Category: Genetics & Stem Cells Authors: Elena Espinosa Rocio Bautista Rafael Larrosa Oscar Plata Source Type: research
Genome sequencing and assembly of Indian golden silkmoth, Antheraea assamensis Helfer (Saturniidae, Lepidoptera)
This study provides valuable insights into the molecular basis of evolutionary divergence and adaptations in muga silkmoth. The genome assembly reported in this study will significantly help in the functional genomics studies on A. assamensis and other Antheraea species along with comparative genomics analyses of Bombycoidea insects.PMID:38599255 | DOI:10.1016/j.ygeno.2024.110841 (Source: Genomics)
Source: Genomics - April 10, 2024 Category: Genetics & Stem Cells Authors: Himanshu Dubey A R Pradeep Kartik Neog Rajal Debnath P J Aneesha Suraj Kumar Shah Indumathi Kamatchi K M Ponnuvel A Ramesha Kunjupillai Vijayan Upendra Nongthomba Utpal Bora Sivaprasad Vankadara K M VijayaKumari Kallare P Arunkumar Source Type: research
Genome sequencing and assembly of Indian golden silkmoth, Antheraea assamensis Helfer (Saturniidae, Lepidoptera)
This study provides valuable insights into the molecular basis of evolutionary divergence and adaptations in muga silkmoth. The genome assembly reported in this study will significantly help in the functional genomics studies on A. assamensis and other Antheraea species along with comparative genomics analyses of Bombycoidea insects.PMID:38599255 | DOI:10.1016/j.ygeno.2024.110841 (Source: Genomics)
Source: Genomics - April 10, 2024 Category: Genetics & Stem Cells Authors: Himanshu Dubey A R Pradeep Kartik Neog Rajal Debnath P J Aneesha Suraj Kumar Shah Indumathi Kamatchi K M Ponnuvel A Ramesha Kunjupillai Vijayan Upendra Nongthomba Utpal Bora Sivaprasad Vankadara K M VijayaKumari Kallare P Arunkumar Source Type: research
Genome sequencing and assembly of Indian golden silkmoth, Antheraea assamensis Helfer (Saturniidae, Lepidoptera)
This study provides valuable insights into the molecular basis of evolutionary divergence and adaptations in muga silkmoth. The genome assembly reported in this study will significantly help in the functional genomics studies on A. assamensis and other Antheraea species along with comparative genomics analyses of Bombycoidea insects.PMID:38599255 | DOI:10.1016/j.ygeno.2024.110841 (Source: Genomics)
Source: Genomics - April 10, 2024 Category: Genetics & Stem Cells Authors: Himanshu Dubey A R Pradeep Kartik Neog Rajal Debnath P J Aneesha Suraj Kumar Shah Indumathi Kamatchi K M Ponnuvel A Ramesha Kunjupillai Vijayan Upendra Nongthomba Utpal Bora Sivaprasad Vankadara K M VijayaKumari Kallare P Arunkumar Source Type: research
Variants in FOXC1 and FOXC2 identified in patients with conotruncal heart defects
Genomics. 2024 Apr 3;116(3):110840. doi: 10.1016/j.ygeno.2024.110840. Online ahead of print.ABSTRACTConotruncal heart defects (CTD), subtypes of congenital heart disease, result from abnormal cardiac outflow tract development (OFT). FOXC1 and FOXC2 are closely related members of the forkhead transcription factor family and play essential roles in the development of OFT. We confirmed their expression pattern in mouse and human embryos, identifying four variants in FOXC1 and three in FOXC2 by screening these two genes in 605 patients with sporadic CTD. Western blot demonstrated expression levels, while Dual-luciferase report...
Source: Genomics - April 5, 2024 Category: Genetics & Stem Cells Authors: Wei Wei Bojian Li Fen Li Kun Sun Xuechao Jiang Rang Xu Source Type: research
Variants in FOXC1 and FOXC2 identified in patients with conotruncal heart defects
Genomics. 2024 Apr 3;116(3):110840. doi: 10.1016/j.ygeno.2024.110840. Online ahead of print.ABSTRACTConotruncal heart defects (CTD), subtypes of congenital heart disease, result from abnormal cardiac outflow tract development (OFT). FOXC1 and FOXC2 are closely related members of the forkhead transcription factor family and play essential roles in the development of OFT. We confirmed their expression pattern in mouse and human embryos, identifying four variants in FOXC1 and three in FOXC2 by screening these two genes in 605 patients with sporadic CTD. Western blot demonstrated expression levels, while Dual-luciferase report...
Source: Genomics - April 5, 2024 Category: Genetics & Stem Cells Authors: Wei Wei Bojian Li Fen Li Kun Sun Xuechao Jiang Rang Xu Source Type: research
Variants in FOXC1 and FOXC2 identified in patients with conotruncal heart defects
Genomics. 2024 Apr 3:110840. doi: 10.1016/j.ygeno.2024.110840. Online ahead of print.ABSTRACTConotruncal heart defects (CTD), subtypes of congenital heart disease, result from abnormal cardiac outflow tract development (OFT). FOXC1 and FOXC2 are closely related members of the forkhead transcription factor family and play essential roles in the development of OFT. We confirmed their expression pattern in mouse and human embryos, identifying four variants in FOXC1 and three in FOXC2 by screening these two genes in 605 patients with sporadic CTD. Western blot demonstrated expression levels, while Dual-luciferase reporter assa...
Source: Genomics - April 5, 2024 Category: Genetics & Stem Cells Authors: Wei Wei Bojian Li Fen Li Kun Sun Xuechao Jiang Rang Xu Source Type: research
