Exosomes derived from ovarian cancer cells regulate proliferation and migration of cancer-associated fibroblasts
This study identified H19/miR-29c-3p/LOXL2-COL1A1 as a ceRNA regulatory network involved in regulating tumour matrix-associated signaling pathways associated with CAF. Cellular assays demonstrated that exosomes from ovarian cancer cell line SKOV3 significantly promoted the proliferation and migration of CAF. The results of mixed transplantation tumour experiments in nude mice showed that exosomes of SKOV3 significantly promoted tumour growth. Ovarian cancer tumour-derived exosomes can regulate CAF proliferation and migration through H19/miR-29c-3p/LOXL2-COL1A1. This study reveals the regulatory role of tumour exosomes on C...
Source: Genomics - September 7, 2023 Category: Genetics & Stem Cells Authors: Bo Ding Zheng Ye Han Yin Xin-Yi Hong Song-Wei Feng Jing-Yun Xu Yang Shen Source Type: research

N6-methyladenosine-modified microRNA-675 advances the development of gastrointestinal stromal tumors via inhibiting myosin phosphatase targeting protein 1
Genomics. 2023 Sep 5:110704. doi: 10.1016/j.ygeno.2023.110704. Online ahead of print.ABSTRACTRNA N6-methyladenosine (m6A) modifications influence gastrointestinal stromal tumors (GISTs) development, but the detailed molecular mechanisms have not been fully studied. Here, microRNA-675 was found to be aberrantly elevated in cancerous tissues and cells of GISTs, compared to the corresponding normal counterparts, and GISTs patients with high-expressed microRNA-675 have worse outcomes. Additional experiments confirmed that silencing of microRNA-675 hindered cell division, mobility and tumorigenesis in vitro and in vivo, whereas...
Source: Genomics - September 7, 2023 Category: Genetics & Stem Cells Authors: Xiaohua Ling Ruifeng Wang Luoqiang Lin Yuxuan Wu Weipeng Cheng Source Type: research

Exosomes derived from ovarian cancer cells regulate proliferation and migration of cancer-associated fibroblasts
This study identified H19/miR-29c-3p/LOXL2-COL1A1 as a ceRNA regulatory network involved in regulating tumour matrix-associated signaling pathways associated with CAF. Cellular assays demonstrated that exosomes from ovarian cancer cell line SKOV3 significantly promoted the proliferation and migration of CAF. The results of mixed transplantation tumour experiments in nude mice showed that exosomes of SKOV3 significantly promoted tumour growth. Ovarian cancer tumour-derived exosomes can regulate CAF proliferation and migration through H19/miR-29c-3p/LOXL2-COL1A1. This study reveals the regulatory role of tumour exosomes on C...
Source: Genomics - September 7, 2023 Category: Genetics & Stem Cells Authors: Bo Ding Zheng Ye Han Yin Xin-Yi Hong Song-Wei Feng Jing-Yun Xu Yang Shen Source Type: research

N6-methyladenosine-modified microRNA-675 advances the development of gastrointestinal stromal tumors via inhibiting myosin phosphatase targeting protein 1
Genomics. 2023 Sep 5:110704. doi: 10.1016/j.ygeno.2023.110704. Online ahead of print.ABSTRACTRNA N6-methyladenosine (m6A) modifications influence gastrointestinal stromal tumors (GISTs) development, but the detailed molecular mechanisms have not been fully studied. Here, microRNA-675 was found to be aberrantly elevated in cancerous tissues and cells of GISTs, compared to the corresponding normal counterparts, and GISTs patients with high-expressed microRNA-675 have worse outcomes. Additional experiments confirmed that silencing of microRNA-675 hindered cell division, mobility and tumorigenesis in vitro and in vivo, whereas...
Source: Genomics - September 7, 2023 Category: Genetics & Stem Cells Authors: Xiaohua Ling Ruifeng Wang Luoqiang Lin Yuxuan Wu Weipeng Cheng Source Type: research

Exosomes derived from ovarian cancer cells regulate proliferation and migration of cancer-associated fibroblasts
This study identified H19/miR-29c-3p/LOXL2-COL1A1 as a ceRNA regulatory network involved in regulating tumour matrix-associated signaling pathways associated with CAF. Cellular assays demonstrated that exosomes from ovarian cancer cell line SKOV3 significantly promoted the proliferation and migration of CAF. The results of mixed transplantation tumour experiments in nude mice showed that exosomes of SKOV3 significantly promoted tumour growth. Ovarian cancer tumour-derived exosomes can regulate CAF proliferation and migration through H19/miR-29c-3p/LOXL2-COL1A1. This study reveals the regulatory role of tumour exosomes on C...
Source: Genomics - September 7, 2023 Category: Genetics & Stem Cells Authors: Bo Ding Zheng Ye Han Yin Xin-Yi Hong Song-Wei Feng Jing-Yun Xu Yang Shen Source Type: research

N6-methyladenosine-modified microRNA-675 advances the development of gastrointestinal stromal tumors via inhibiting myosin phosphatase targeting protein 1
Genomics. 2023 Sep 5:110704. doi: 10.1016/j.ygeno.2023.110704. Online ahead of print.ABSTRACTRNA N6-methyladenosine (m6A) modifications influence gastrointestinal stromal tumors (GISTs) development, but the detailed molecular mechanisms have not been fully studied. Here, microRNA-675 was found to be aberrantly elevated in cancerous tissues and cells of GISTs, compared to the corresponding normal counterparts, and GISTs patients with high-expressed microRNA-675 have worse outcomes. Additional experiments confirmed that silencing of microRNA-675 hindered cell division, mobility and tumorigenesis in vitro and in vivo, whereas...
Source: Genomics - September 7, 2023 Category: Genetics & Stem Cells Authors: Xiaohua Ling Ruifeng Wang Luoqiang Lin Yuxuan Wu Weipeng Cheng Source Type: research

Exosomes derived from ovarian cancer cells regulate proliferation and migration of cancer-associated fibroblasts
This study identified H19/miR-29c-3p/LOXL2-COL1A1 as a ceRNA regulatory network involved in regulating tumour matrix-associated signaling pathways associated with CAF. Cellular assays demonstrated that exosomes from ovarian cancer cell line SKOV3 significantly promoted the proliferation and migration of CAF. The results of mixed transplantation tumour experiments in nude mice showed that exosomes of SKOV3 significantly promoted tumour growth. Ovarian cancer tumour-derived exosomes can regulate CAF proliferation and migration through H19/miR-29c-3p/LOXL2-COL1A1. This study reveals the regulatory role of tumour exosomes on C...
Source: Genomics - September 7, 2023 Category: Genetics & Stem Cells Authors: Bo Ding Zheng Ye Han Yin Xin-Yi Hong Song-Wei Feng Jing-Yun Xu Yang Shen Source Type: research

N6-methyladenosine-modified microRNA-675 advances the development of gastrointestinal stromal tumors via inhibiting myosin phosphatase targeting protein 1
Genomics. 2023 Sep 5:110704. doi: 10.1016/j.ygeno.2023.110704. Online ahead of print.ABSTRACTRNA N6-methyladenosine (m6A) modifications influence gastrointestinal stromal tumors (GISTs) development, but the detailed molecular mechanisms have not been fully studied. Here, microRNA-675 was found to be aberrantly elevated in cancerous tissues and cells of GISTs, compared to the corresponding normal counterparts, and GISTs patients with high-expressed microRNA-675 have worse outcomes. Additional experiments confirmed that silencing of microRNA-675 hindered cell division, mobility and tumorigenesis in vitro and in vivo, whereas...
Source: Genomics - September 7, 2023 Category: Genetics & Stem Cells Authors: Xiaohua Ling Ruifeng Wang Luoqiang Lin Yuxuan Wu Weipeng Cheng Source Type: research

Clinical characterization and immunosuppressive regulation role of EFHD2 (swiprosin-1) in Glioma-associated macrophages
In conclusion, EFHD2 promises to be a key target for TME-related immunotherapy.PMID:37673235 | DOI:10.1016/j.ygeno.2023.110702 (Source: Genomics)
Source: Genomics - September 6, 2023 Category: Genetics & Stem Cells Authors: Wen-Zhao Zhang Lin-Lin Chen Shuo Yang Yu-Ting Zhong Xin Lu Yan Wang Zhi-Bin Wang Ye Tu Source Type: research

Clinical characterization and immunosuppressive regulation role of EFHD2 (swiprosin-1) in Glioma-associated macrophages
In conclusion, EFHD2 promises to be a key target for TME-related immunotherapy.PMID:37673235 | DOI:10.1016/j.ygeno.2023.110702 (Source: Genomics)
Source: Genomics - September 6, 2023 Category: Genetics & Stem Cells Authors: Wen-Zhao Zhang Lin-Lin Chen Shuo Yang Yu-Ting Zhong Xin Lu Yan Wang Zhi-Bin Wang Ye Tu Source Type: research

Comparing assembly strategies for third-generation sequencing technologies across different genomes
Genomics. 2023 Aug 18;115(5):110700. doi: 10.1016/j.ygeno.2023.110700. Online ahead of print.ABSTRACTThe recent advent of long-read sequencing technologies, such as Pacific Biosciences (PacBio) and Oxford Nanopore technology (ONT), has led to substantial accuracy and computational cost improvements. However, de novo whole-genome assembly still presents significant challenges related to the computational cost and the quality of the results. Accordingly, sequencing accuracy and throughput continue to improve, and many tools are constantly emerging. Therefore, selecting the correct sequencing platform, the proper sequencing d...
Source: Genomics - August 20, 2023 Category: Genetics & Stem Cells Authors: Elena Espinosa Rocio Bautista Ivan Fernandez Rafael Larrosa Emilio L Zapata Oscar Plata Source Type: research

Comparing assembly strategies for third-generation sequencing technologies across different genomes
Genomics. 2023 Aug 18;115(5):110700. doi: 10.1016/j.ygeno.2023.110700. Online ahead of print.ABSTRACTThe recent advent of long-read sequencing technologies, such as Pacific Biosciences (PacBio) and Oxford Nanopore technology (ONT), has led to substantial accuracy and computational cost improvements. However, de novo whole-genome assembly still presents significant challenges related to the computational cost and the quality of the results. Accordingly, sequencing accuracy and throughput continue to improve, and many tools are constantly emerging. Therefore, selecting the correct sequencing platform, the proper sequencing d...
Source: Genomics - August 20, 2023 Category: Genetics & Stem Cells Authors: Elena Espinosa Rocio Bautista Ivan Fernandez Rafael Larrosa Emilio L Zapata Oscar Plata Source Type: research

Comparing assembly strategies for third-generation sequencing technologies across different genomes
Genomics. 2023 Aug 18;115(5):110700. doi: 10.1016/j.ygeno.2023.110700. Online ahead of print.ABSTRACTThe recent advent of long-read sequencing technologies, such as Pacific Biosciences (PacBio) and Oxford Nanopore technology (ONT), has led to substantial accuracy and computational cost improvements. However, de novo whole-genome assembly still presents significant challenges related to the computational cost and the quality of the results. Accordingly, sequencing accuracy and throughput continue to improve, and many tools are constantly emerging. Therefore, selecting the correct sequencing platform, the proper sequencing d...
Source: Genomics - August 20, 2023 Category: Genetics & Stem Cells Authors: Elena Espinosa Rocio Bautista Ivan Fernandez Rafael Larrosa Emilio L Zapata Oscar Plata Source Type: research

Comparing assembly strategies for third-generation sequencing technologies across different genomes
Genomics. 2023 Aug 18;115(5):110700. doi: 10.1016/j.ygeno.2023.110700. Online ahead of print.ABSTRACTThe recent advent of long-read sequencing technologies, such as Pacific Biosciences (PacBio) and Oxford Nanopore technology (ONT), has led to substantial accuracy and computational cost improvements. However, de novo whole-genome assembly still presents significant challenges related to the computational cost and the quality of the results. Accordingly, sequencing accuracy and throughput continue to improve, and many tools are constantly emerging. Therefore, selecting the correct sequencing platform, the proper sequencing d...
Source: Genomics - August 20, 2023 Category: Genetics & Stem Cells Authors: Elena Espinosa Rocio Bautista Ivan Fernandez Rafael Larrosa Emilio L Zapata Oscar Plata Source Type: research

Comparing assembly strategies for third-generation sequencing technologies across different genomes
Genomics. 2023 Aug 18;115(5):110700. doi: 10.1016/j.ygeno.2023.110700. Online ahead of print.ABSTRACTThe recent advent of long-read sequencing technologies, such as Pacific Biosciences (PacBio) and Oxford Nanopore technology (ONT), has led to substantial accuracy and computational cost improvements. However, de novo whole-genome assembly still presents significant challenges related to the computational cost and the quality of the results. Accordingly, sequencing accuracy and throughput continue to improve, and many tools are constantly emerging. Therefore, selecting the correct sequencing platform, the proper sequencing d...
Source: Genomics - August 20, 2023 Category: Genetics & Stem Cells Authors: Elena Espinosa Rocio Bautista Ivan Fernandez Rafael Larrosa Emilio L Zapata Oscar Plata Source Type: research