Liver transcriptomic and proteomic analyses provide new insight into the pathogenesis of liver fibrosis in mice
CONCLUSIONS: Through the combined analysis of transcriptomic and proteomic data, this study provides valuable insights into the potential mechanism of the pathogenesis of LF, and lays a theoretical foundation for the further development of targeted therapy for LF.PMID:37918454 | DOI:10.1016/j.ygeno.2023.110738 (Source: Genomics)
Source: Genomics - November 2, 2023 Category: Genetics & Stem Cells Authors: Lili Zhang Qiumei Zhou Jiafu Zhang Kefeng Cao Chang Fan Sen Chen Hui Jiang Furong Wu Source Type: research
Mitophagy and mitochondrion-related expression profiles in response to physiological and pathological hypoxia in the corneal epithelium
Genomics. 2023 Oct 31;115(6):110739. doi: 10.1016/j.ygeno.2023.110739. Online ahead of print.ABSTRACTTo study the mitochondrial and cellular responses to physiological and pathological hypoxia, corneal epithelial cells were preconditioned under 21% O2, 8% O2 or 1% O2. The cell survival rate, mitochondrial fluorescence and mitophagy flux were quantified using flow cytometry. After RNA sequencing, gene set enrichment analysis (GSEA) was performed. When the oxygen level decreased from 21% to 8%, mitochondrial fluorescence decreased by 45% (p < 0.001), accompanied by an 80% increase in mitophagy flux (p < 0.001). When th...
Source: Genomics - November 2, 2023 Category: Genetics & Stem Cells Authors: Ming-Feng Wu Xi Peng Jiang-Lan Zhao Ming-Chang Zhang Hua-Tao Xie Source Type: research
Liver transcriptomic and proteomic analyses provide new insight into the pathogenesis of liver fibrosis in mice
CONCLUSIONS: Through the combined analysis of transcriptomic and proteomic data, this study provides valuable insights into the potential mechanism of the pathogenesis of LF, and lays a theoretical foundation for the further development of targeted therapy for LF.PMID:37918454 | DOI:10.1016/j.ygeno.2023.110738 (Source: Genomics)
Source: Genomics - November 2, 2023 Category: Genetics & Stem Cells Authors: Lili Zhang Qiumei Zhou Jiafu Zhang Kefeng Cao Chang Fan Sen Chen Hui Jiang Furong Wu Source Type: research
Mitophagy and mitochondrion-related expression profiles in response to physiological and pathological hypoxia in the corneal epithelium
Genomics. 2023 Oct 31:110739. doi: 10.1016/j.ygeno.2023.110739. Online ahead of print.ABSTRACTTo study the mitochondrial and cellular responses to physiological and pathological hypoxia, corneal epithelial cells were preconditioned under 21% O2, 8% O2 or 1% O2. The cell survival rate, mitochondrial fluorescence and mitophagy flux were quantified using flow cytometry. After RNA sequencing, gene set enrichment analysis (GSEA) was performed. When the oxygen level decreased from 21% to 8%, mitochondrial fluorescence decreased by 45% (p < 0.001), accompanied by an 80% increase in mitophagy flux (p < 0.001). When the oxyge...
Source: Genomics - November 2, 2023 Category: Genetics & Stem Cells Authors: Ming-Feng Wu Xi Peng Jiang-Lan Zhao Ming-Chang Zhang Hua-Tao Xie Source Type: research
Nasal responses to elevated temperature and Francisella noatunensis infection in Atlantic cod (Gadus morhua)
In conclusion, the study reveals that elevated temperature could trigger responses in the olfactory organ of Atlantic cod and shape the nasal response to F. noatunensis infection.PMID:37898334 | DOI:10.1016/j.ygeno.2023.110735 (Source: Genomics)
Source: Genomics - October 28, 2023 Category: Genetics & Stem Cells Authors: Carlo C Lazado Marianne Iversen Lill-Heidi Johansen Hanne Brenne Arvind Y M Sundaram Elisabeth Ytteborg Source Type: research
Nasal responses to elevated temperature and Francisella noatunensis infection in Atlantic cod (Gadus morhua)
In conclusion, the study reveals that elevated temperature could trigger responses in the olfactory organ of Atlantic cod and shape the nasal response to F. noatunensis infection.PMID:37898334 | DOI:10.1016/j.ygeno.2023.110735 (Source: Genomics)
Source: Genomics - October 28, 2023 Category: Genetics & Stem Cells Authors: Carlo C Lazado Marianne Iversen Lill-Heidi Johansen Hanne Brenne Arvind Y M Sundaram Elisabeth Ytteborg Source Type: research
Nasal responses to elevated temperature and Francisella noatunensis infection in Atlantic cod (Gadus morhua)
In conclusion, the study reveals that elevated temperature could trigger responses in the olfactory organ of Atlantic cod and shape the nasal response to F. noatunensis infection.PMID:37898334 | DOI:10.1016/j.ygeno.2023.110735 (Source: Genomics)
Source: Genomics - October 28, 2023 Category: Genetics & Stem Cells Authors: Carlo C Lazado Marianne Iversen Lill-Heidi Johansen Hanne Brenne Arvind Y M Sundaram Elisabeth Ytteborg Source Type: research
Nasal responses to elevated temperature and Francisella noatunensis infection in Atlantic cod (Gadus morhua)
In conclusion, the study reveals that elevated temperature could trigger responses in the olfactory organ of Atlantic cod and shape the nasal response to F. noatunensis infection.PMID:37898334 | DOI:10.1016/j.ygeno.2023.110735 (Source: Genomics)
Source: Genomics - October 28, 2023 Category: Genetics & Stem Cells Authors: Carlo C Lazado Marianne Iversen Lill-Heidi Johansen Hanne Brenne Arvind Y M Sundaram Elisabeth Ytteborg Source Type: research
Genomics evolution of Jingmen viruses associated with ticks and vertebrates
Genomics. 2023 Oct 25:110734. doi: 10.1016/j.ygeno.2023.110734. Online ahead of print.ABSTRACTJingmen virus (JMV) associated with ticks and vertebrates have been found to be related to human disease. We obtained the genome of a Jingmen tick virus (JMTV) strain from Rhipicephalus microplus in Guizhou province and compared the genomes of seven JMV species associated with ticks and vertebrates to understand the evolutionary relationships. The topology of the phylogenetic tree of segment 1 and segment 3 is similar, and segment 2 and segment 4 formed two different topologies, with the main differences being between Alongshan vi...
Source: Genomics - October 27, 2023 Category: Genetics & Stem Cells Authors: Weiyi Li Rongting Li Xiaomin Tang Jinzhi Cheng Lin Zhan Zhengling Shang Jiahong Wu Source Type: research
Genomics evolution of Jingmen viruses associated with ticks and vertebrates
Genomics. 2023 Oct 25:110734. doi: 10.1016/j.ygeno.2023.110734. Online ahead of print.ABSTRACTJingmen virus (JMV) associated with ticks and vertebrates have been found to be related to human disease. We obtained the genome of a Jingmen tick virus (JMTV) strain from Rhipicephalus microplus in Guizhou province and compared the genomes of seven JMV species associated with ticks and vertebrates to understand the evolutionary relationships. The topology of the phylogenetic tree of segment 1 and segment 3 is similar, and segment 2 and segment 4 formed two different topologies, with the main differences being between Alongshan vi...
Source: Genomics - October 27, 2023 Category: Genetics & Stem Cells Authors: Weiyi Li Rongting Li Xiaomin Tang Jinzhi Cheng Lin Zhan Zhengling Shang Jiahong Wu Source Type: research
Deficiency of ligase IV leads to reduced NHEJ, accumulation of DNA damage, and can sensitize cells to cancer therapeutics
Genomics. 2023 Oct 21:110731. doi: 10.1016/j.ygeno.2023.110731. Online ahead of print.ABSTRACTLigase IV is a key enzyme involved during DNA double-strand breaks (DSBs) repair through nonhomologous end joining (NHEJ). However, in contrast to Ligase IV deficient mouse cells, which are embryonic lethal, Ligase IV deficient human cells, including pre-B cells, are viable. Using CRISPR-Cas9 mediated genome editing, we have generated six different LIG4 mutants in cervical cancer and normal kidney epithelial cell lines. While the LIG4 mutant cells showed a significant reduction in NHEJ, joining mediated through microhomology-media...
Source: Genomics - October 23, 2023 Category: Genetics & Stem Cells Authors: Nitu Kumari Himanshu Antil Susmita Kumari Sathees C Raghavan Source Type: research
Deficiency of ligase IV leads to reduced NHEJ, accumulation of DNA damage, and can sensitize cells to cancer therapeutics
Genomics. 2023 Oct 21:110731. doi: 10.1016/j.ygeno.2023.110731. Online ahead of print.ABSTRACTLigase IV is a key enzyme involved during DNA double-strand breaks (DSBs) repair through nonhomologous end joining (NHEJ). However, in contrast to Ligase IV deficient mouse cells, which are embryonic lethal, Ligase IV deficient human cells, including pre-B cells, are viable. Using CRISPR-Cas9 mediated genome editing, we have generated six different LIG4 mutants in cervical cancer and normal kidney epithelial cell lines. While the LIG4 mutant cells showed a significant reduction in NHEJ, joining mediated through microhomology-media...
Source: Genomics - October 23, 2023 Category: Genetics & Stem Cells Authors: Nitu Kumari Himanshu Antil Susmita Kumari Sathees C Raghavan Source Type: research
Deficiency of ligase IV leads to reduced NHEJ, accumulation of DNA damage, and can sensitize cells to cancer therapeutics
Genomics. 2023 Oct 21:110731. doi: 10.1016/j.ygeno.2023.110731. Online ahead of print.ABSTRACTLigase IV is a key enzyme involved during DNA double-strand breaks (DSBs) repair through nonhomologous end joining (NHEJ). However, in contrast to Ligase IV deficient mouse cells, which are embryonic lethal, Ligase IV deficient human cells, including pre-B cells, are viable. Using CRISPR-Cas9 mediated genome editing, we have generated six different LIG4 mutants in cervical cancer and normal kidney epithelial cell lines. While the LIG4 mutant cells showed a significant reduction in NHEJ, joining mediated through microhomology-media...
Source: Genomics - October 23, 2023 Category: Genetics & Stem Cells Authors: Nitu Kumari Himanshu Antil Susmita Kumari Sathees C Raghavan Source Type: research
Deficiency of ligase IV leads to reduced NHEJ, accumulation of DNA damage, and can sensitize cells to cancer therapeutics
Genomics. 2023 Oct 21:110731. doi: 10.1016/j.ygeno.2023.110731. Online ahead of print.ABSTRACTLigase IV is a key enzyme involved during DNA double-strand breaks (DSBs) repair through nonhomologous end joining (NHEJ). However, in contrast to Ligase IV deficient mouse cells, which are embryonic lethal, Ligase IV deficient human cells, including pre-B cells, are viable. Using CRISPR-Cas9 mediated genome editing, we have generated six different LIG4 mutants in cervical cancer and normal kidney epithelial cell lines. While the LIG4 mutant cells showed a significant reduction in NHEJ, joining mediated through microhomology-media...
Source: Genomics - October 23, 2023 Category: Genetics & Stem Cells Authors: Nitu Kumari Himanshu Antil Susmita Kumari Sathees C Raghavan Source Type: research
RIOK3 potentially regulates osteogenesis-related pathways in ankylosing spondylitis and the differentiation of bone marrow mesenchymal stem cells
Genomics. 2023 Oct 20:110730. doi: 10.1016/j.ygeno.2023.110730. Online ahead of print.ABSTRACTRNA-binding proteins (RBPs), which are key effectors of gene expression, play critical roles in inflammation and immune regulation. However, the potential biological function of RBPs in ankylosing spondylitis (AS) remains unclear. We identified differentially expressed genes (DEGs) in peripheral blood mononuclear cells (PBMCs) of five patients with AS and three healthy persons by RNA-seq, obtained differentially expressed RBPs by overlapping DEGs and RBPs summary table. RIOK3 was selected as a target RBP and knocked down in mouse ...
Source: Genomics - October 22, 2023 Category: Genetics & Stem Cells Authors: He-Xiang Zong Ya-Qian Liu Xi-le Wang Jie-Yu Miao Li-Ping Luo Jian-Xiong Wang Yi-Ran Chu Wan-Qiu Tong Xu Zhao Sheng-Qian Xu Source Type: research
