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Specialty: Biomedical Science

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Total 61743 results found since Jan 2013.

Down-regulation of XIAP enhances the radiosensitivity of esophageal cancer cells in vivo and in vitro.
Abstract The study investigated the effects of XIAP gene silencing on the radiosensitivity of esophageal cancer (EC) cells. Western blotting was used to select EC cell lines with XIAP overexpression. Selected EC9706 and KYSE30 cell lines were both divided into four groups: the blank control group, the negative control (NC) group (transfected with pBSHH1), the siRNA-enhanced group (transfected with pBSHH1-XIAP1-siRNA), and the siRNA-decreased group (transfected with pBSHH1-XIAP2-siRNA). Expressions of XIAP were measured by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting, ...
Source: Bioscience Reports - August 18, 2017 Category: Biomedical Science Authors: Wen X, Han XR, Fan SH, Zhang ZF, Chen L, Yi G, Wu DM, Lu J, Zheng YL Tags: Biosci Rep Source Type: research

Research Articles Targeted fibrillar nanocarbon RNAi treatment of acute kidney injury
RNA interference has tremendous yet unrealized potential to treat a wide range of illnesses. Innovative solutions are needed to protect and selectively deliver small interfering RNA (siRNA) cargo to and within a target cell to fully exploit siRNA as a therapeutic tool in vivo. Herein, we describe ammonium-functionalized carbon nanotube (fCNT)–mediated transport of siRNA selectively and with high efficiency to renal proximal tubule cells in animal models of acute kidney injury (AKI). fCNT enhanced siRNA delivery to tubule cells compared to siRNA alone and effectively knocked down the expression of several target genes...
Source: Science Translational Medicine - March 22, 2016 Category: Biomedical Science Authors: Alidori, S., Akhavein, N., Thorek, D. L. J., Behling, K., Romin, Y., Queen, D., Beattie, B. J., Manova-Todorova, K., Bergkvist, M., Scheinberg, D. A., McDevitt, M. R. Tags: Research Articles Source Type: research

Target gene delivery from targeting ligand conjugated chitosan–PEI copolymer for cancer therapy
In this study, we designed a novel carrier which was having low cytotoxicity, site-specific target function, and high transfection efficiency using low molecular weight water soluble O-carboxymethyl chitosan (OCMCh), branched low molecular weight poly(ethyleneimine) (bPEI), and targeting ligand (epitope type, HER-2/neu). OCMCh/bPEI/targeting ligand, HPOCP copolymer, and targeting ligand-modified polyamphoteric polymer, and were prepared by chemical reaction and characterized by 1H NMR and FT-IR. The binding affinity, protecting efficiency, and releasing ability of gene/HPOCP polyplex were confirmed by gel retardation assay...
Source: Carbohydrate Polymers - September 9, 2015 Category: Biomedical Science Source Type: research

Silencing of myeloid cell leukemia-1 by small interfering RNA improves chemosensitivity to etoposide in u-937 leukemic cells.
Authors: Jafarlou M, Baradaran B, Shanehbandi D, Saedi TA, Jafarlou V, Karimi P, Othman F Abstract A key issue in the treatment of acute myeloid leukemia (AML) is the development of drug resistance to chemotherapeutic agents. Overexpression of myeloid cell leukemia-1 (Mcl-1), an anti-apoptotic protein, is associated with tumor progression and drug resistance in leukemia and several cancers. The purpose of this study was to investigate the effect of specific Mcl-1 small interference RNA (siRNA) on the proliferation and chemosensitivity of U-937 AML cell to etoposide. The siRNA transfection was conducted using Lipofe...
Source: Journal of Biological Regulators and Homeostatic Agents - April 8, 2016 Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research

Effects of microRNA-136 on melanoma cell proliferation, apoptosis and epithelial mesenchymal transition by targeting PMEL through the Wnt signaling pathway.
Abstract The study aims to evaluate the effects of miR-136 on the proliferation, apoptosis and epithelial mesenchymal transition (EMT) of melanoma cells by targeting PMEL through the Wnt signaling pathway. After establishment of melanoma mouse models, melanoma (model group) and normal tissues (normal group) were collected. Immunohistochemistry was performed to determine PMEL protein concentration. Mouse melanoma cells were assigned into control, blank, negative control (NC), miR-136 mimics, miR-136 inhibitors, siRNA-PMEL, and miR-136 inhibitors + siRNA-PMEL, LiC1 (Wnt signaling pathway activator) and siRNA-PMEL+ L...
Source: Bioscience Reports - July 19, 2017 Category: Biomedical Science Authors: Wang JJ, Li ZF, Li XJ, Han Z, Zhang L, Liu ZJ Tags: Biosci Rep Source Type: research

Knockdown of human telomerase reverse transcriptase induces apoptosis in cervical cancer cell line.
Abstract Background & objectives: : Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of telomerase enzyme that maintains telomere ends by the addition of telomeric repeats to the ends of chromosomal DNA, and that may generate immortal cancer cells. Hence, the activity of telomerase is raised in cancer cells including cervical cancer. The present study aimed to validate the unique siRNA loaded chitosan coated poly-lactic-co-glycolic acid (PLGA) nanoparticle targeting hTERT mRNA to knock down the expression of hTERT in HeLa cells. Methods: : The siRNA loaded chitosan coated polylactic...
Source: The Indian Journal of Medical Research - February 28, 2019 Category: Biomedical Science Authors: Nagapoosanam AL, Ganesan N, Umapathy D, Moorthy RK, Arockiam AJV Tags: Indian J Med Res Source Type: research

Dexmedetomidine reverses MTX-induced neurotoxicity and inflammation in hippocampal HT22 cell lines via NCOA4-mediated ferritinophagy
Aging (Albany NY). 2021 Feb 25;13. doi: 10.18632/aging.202626. Online ahead of print.ABSTRACTThe incidence of chemotherapy-induced cognitive impairment (CICI) has attracted massive attention. Some studies have demonstrated the neuroprotective effects of dexmedetomidine (DEX). Here, alterations in nuclear receptor coactivator 4 (NCOA4)-mediated ferritinophagy were investigated as the possible causes of DEX's neuroprotection of HT22 cells against methotrexate (MTX)-induced neurotoxicity. We used various concentrations of DEX and NCOA4-siRNA to treat MTX-induced neurotoxicity and inflammation in HT22 cells. The biomarkers of ...
Source: Aging - February 26, 2021 Category: Biomedical Science Authors: Jingli Chen Juan Wang Chenxi Li Huang Ding Jishi Ye Zhongyuan Xia Source Type: research

Silencing efficacy prediction: a retrospective study on target mRNA features.
Abstract Post-transcriptional gene silencing is a widely used method to suppress gene expression. Unfortunately only a portion of siRNAs do successfully reduce gene expression. Target mRNA secondary structures and siRNA/RNA thermodynamic features are believed to contribute to the silencing activity. However, there is still an open discussion as to what determines siRNA efficacy. In this retrospective study, we analyzed the target accessibility comparing very high vs. low efficacy siRNA sequences obtained from the siRecords Database. We determined the contribution of mRNA target local secondary structures on silenc...
Source: Bioscience Reports - February 23, 2015 Category: Biomedical Science Authors: Pascut D, Bedogni G, Tiribelli C Tags: Biosci Rep Source Type: research

Lipopolysaccharide promoted proliferation and adipogenesis of preadipocytes through JAK/STAT and AMPK-regulated cPLA2 expression.
Authors: Chang CC, Sia KC, Chang JF, Lin CM, Yang CM, Huang KY, Lin WN Abstract The proliferation and adipogenesis of preadipocytes played important roles in the development of adipose tissue and contributed much to the processes of obesity. On the other hand, lipopolysaccharide (LPS), also known as endotoxin, is a key outer membrane component of gram-negative bacteria in the gut microbiota, and has a dominant role in linking inflammation to high-fat diet-induced metabolic syndrome. Studies suggested the potential roles of LPS in hepatic steatosis and in obese mice models. However, the molecular mechanisms underlyi...
Source: International Journal of Medical Sciences - January 22, 2019 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

LncRNA ANRIL affects the sensitivity of ovarian cancer to cisplatin via regulation of let-7a/HMGA2 axis.
Abstract This paper tried to explore ANRIL expression in ovarian cancer and how it affects cisplatin-sensitivity of ovarian cancer cells via regulation of let-7a/HMGA2 axis. qRT-PCR was used to detect ANRIL and let-7a levels in ovarian cancer tissues and cell lines (SKOV3 and SKOV3/DDP). Then cells were randomly assigned into Blank, NC siRNA, ANRIL siRNA, let-7a inhibitor, and ANRIL siRNA+let-7a inhibitor groups. CCK-8 assay was applied for assessing cell viability of cells treated with different concentrations of cisplatin . Flow cytometry was employed to test cell apoptosis rate. QRT-PCR and Western blot were pe...
Source: Bioscience Reports - June 11, 2019 Category: Biomedical Science Authors: Miao JT, Gao JH, Chen YQ, Chen H, Meng HY, Lou G Tags: Biosci Rep Source Type: research

Involvement of K+ channel-dependant pathways in lipoxin A4-induced protective effects on hypoxia/reoxygenation injury of cardiomyocytes
In conclusion, the protective role of LXA4 on cardiomyocytes against H/R injury is related to upregulation of HO-1 via reduced production of TNF-α and activation of ATP-sensitive K+ channels and calcium-sensitive K+ channel.
Source: Prostaglandins, Leukotrienes and Essential Fatty Acids - April 22, 2013 Category: Biomedical Science Authors: Xiao-Qing Chen, Sheng-Hua Wu, Yu Zhou, Yan-Rong Tang Tags: Original Articles Source Type: research

Transfer printing of transfected cell microarrays from poly(ethylene glycol)‐oleyl surfaces onto biological hydrogels
Abstract We have developed a novel technique for constructing microarrays of transfected mammalian cells on or in extracellular matrix (ECM) hydrogels by transfer printing from patterned poly(ethylene glycol) (PEG)‐oleyl surfaces. A mixed solution of small interfering RNA (siRNA) and a transfection reagent was spotted on PEG‐oleyl‐coated glass slides using an inkjet printer, and the cells were then transiently immobilized on the patterned transfection mixtures. After overlaying an ECM hydrogel sheet onto the immobilized cells, the cells sandwiched between the glass slide and the hydrogel sheet were incubated at 37°C...
Source: Biotechnology and Bioengineering - July 28, 2013 Category: Biomedical Science Authors: Satoshi Yamaguchi, Senori Komiya, Erika Matsunuma, Shinya Yamahira, Takanori Kihara, Jun Miyake, Teruyuki Nagamune Tags: Communication to the Editor Source Type: research

Aptamer-targeted inhibition of mTOR in T cells enhances antitumor immunity
Recent studies have underscored the importance of memory T cells in mediating protective immunity against pathogens and cancer. Pharmacological inhibition of regulators that mediate T cell differentiation promotes the differentiation of activated CD8+ T cells into memory cells. Nonetheless, pharmacological agents have broad targets and can induce undesirable immunosuppressive effects. Here, we tested the hypothesis that aptamer-targeted siRNA inhibition of mTOR complex 1 (mTORC1) function in CD8+ T cells can enhance their differentiation into memory T cells and potentiate antitumor immunity more effectively than the pharma...
Source: Journal of Clinical Investigation - December 4, 2013 Category: Biomedical Science Authors: Alexey Berezhnoy, Iris Castro, Agata Levay, Thomas R. Malek, Eli Gilboa Source Type: research