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Specialty: Allergy & Immunology

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Total 72221 results found since Jan 2013.

Efficient Nontoxic Delivery of PD-L1 and PD-L2 siRNA Into Dendritic Cell Vaccines Using the Cationic Lipid SAINT-18
Dendritic cell (DC)-based vaccination is an appealing strategy to boost graft-versus-tumor immunity after allogeneic stem cell transplantation (allo-SCT), and thereby prevent or counteract tumor recurrence. By exploiting minor histocompatibility antigens (MiHA) presented on hematopoietic cells, donor CD8+ T-cell immunity can be selectively targeted to patient’s hematological tumor cells without the risk of inducing graft-versus-host disease. Previously, we demonstrated that silencing RNA (siRNA) of programmed death-ligand 1 (PD-L1) and PD-L2 on DCs markedly augments the expansion and function of MiHA-specific CD8+ T cell...
Source: Journal of Immunotherapy - April 16, 2015 Category: Allergy & Immunology Tags: Basic Studies Source Type: research

Nanoparticle-Delivered IRF5 siRNA Facilitates M1 to M2 Transition, Reduces Demyelination and Neurofilament Loss, and Promotes Functional Recovery After Spinal Cord Injury in Mice
ABSTRACT Macrophage activation and persistent inflammation contribute to the pathogenesis of spinal cord injury (SCI), and different phenotypes of macrophages play diverse roles in the pathological process of SCI. After SCI, there is an acute phase of alternatively activated (M2) macrophage infiltration, followed by a long-lasting phase of classically activated (M1) macrophage accumulation in the wound. The long-lasting predominance of M1 macrophages may derail healing and compromise organ functions. Based on the previous findings that the transcription factor interferon regulatory factor 5 (IRF5) up-regulates gen...
Source: Inflammation - July 18, 2016 Category: Allergy & Immunology Source Type: research

High-dose BAFF receptor specific mAb-siRNA conjugate generates Fas-expressing B cells in lymph nodes and high-affinity serum autoantibody in a myasthenia mouse model.
Abstract We investigated potential therapeutic effects of a conjugate of BAFF receptor specific- monoclonal antibody and short interference RNA in a mouse model of myasthenia gravis (EAMG). Whereas high-dose siRNA conjugate resulted in significant accumulation of Fas expressing CD19+/ B220+ cells and concurrent expression of type 1 interferon in lymph nodes, low-dose conjugate did not induce FAS expression but caused marked BAFF receptor deficiency in lymph nodes that was further associated with improved MG symptoms. Unexpectedly, despite inhibiting BAFF receptor significantly in PBMCs and secondary lymphoid organ...
Source: Clinical Immunology - January 14, 2017 Category: Allergy & Immunology Authors: Ibtehaj N, Anderson KE, Huda R Tags: Clin Immunol Source Type: research

siRNA-mediated c-Rel knockdown ameliorates collagen-induced arthritis in mice.
Abstract Previous studies have shown that inflammatory mediators involved in the development of rheumatoid arthritis (RA) are regulated by the Rel/nuclear factor-κB (Rel/NF-κB) transcription factor family. c-Rel, a member of the Rel/NF-κB family that is preferentially expressed by immune cells, is a risk factor for several inflammatory diseases including RA. In the current study, we investigated whether targeting c-Rel can be used to treat collagen-induced arthritis, an animal model for RA. c-Rel specific siRNA (siRel) delivered by nanoparticles was used to knockdown the expression of c-Rel. Our results showed ...
Source: International Immunopharmacology - January 8, 2018 Category: Allergy & Immunology Authors: Fan T, Zhong F, Liu R, Chen YH, Wang T, Ruan Q Tags: Int Immunopharmacol Source Type: research

Targeted delivery of CD44s-siRNA by ScFv overcomes de novo resistance to cetuximab in triple negative breast cancer
In this study, we show that impaired EGFR degradation is crucial for resistance to cetuximab, which depends on the cell surface molecule CD44. To further investigate the role of CD44 in EGFR signaling and its treatment potential, we developed a targeting fusion protein composed of an anti-EGFR scFv generated from cetuximab and truncated protamine, called Ce-tP. CD44 siRNA can be specifically delivered into EGFR-positive TNBC cells by Ce-tP. Efficient knockdown of CD44 and suppression of both EGFR and downstream signaling by the Ce-tP/siRNA complex were observed in EGFR-positive TNBC cells. More importantly, our results als...
Source: Molecular Immunology - May 17, 2018 Category: Allergy & Immunology Source Type: research

PreS/2-21-Guided siRNA Nanoparticles Target to Inhibit Hepatitis B Virus Infection and Replication
A viable therapy is needed to overcome the deadlock of the incurable chronic hepatitis B (CHB). The prolonged existence of covalently closed circular DNA (cccDNA) and integrated HBV DNA in the nucleus of hepatocytes is the root cause of CHB. As a result, it is critical to successfully suppress HBV DNA replication and eliminate cccDNA. RNA interference has been proven in recent research to silence the expression of target genes and thereby decrease HBV replication. However, siRNA is susceptible to be degraded by RNA enzymes in vivo, making it difficult to deliver successfully and lacking of tissue targeting. To exploit the ...
Source: Frontiers in Immunology - April 29, 2022 Category: Allergy & Immunology Source Type: research

Engineered NF- κB siRNA-encapsulating exosomes as a modality for therapy of skin lesions
DiscussionOverall, this study establishes a feasible therapeutic strategy for skin injury, which may offer an alternative to conventional biological therapies requiring two or more independent compounds.
Source: Frontiers in Immunology - February 8, 2023 Category: Allergy & Immunology Source Type: research

siRNA-based identification of IBD-related targets in human monocyte-derived dendritic cells
J Immunol Methods. 2021 Apr 20:113058. doi: 10.1016/j.jim.2021.113058. Online ahead of print.ABSTRACTInflammatory bowel disease (IBD) is thought to be caused by an aberrant host response to the commensal enteric flora in genetically susceptible individuals. Dendritic cells (DCs) play a key role in the regulation of this response as they sample gut commensals. In healthy individuals DCs actively contribute to tolerance upon recognition of these resident bacteria, whereas in individuals with IBD, DCs will initiate an inflammatory response. To mimic the disease response in vitro, human monocyte-derived DCs were matured with E...
Source: Journal of Immunological Methods - April 23, 2021 Category: Allergy & Immunology Authors: Diane Bosman-Schluep Remko de Pril Banu Verbaken Ana ïs Legent Jan Stallen Esther C de Jong Richard A J Janssen Source Type: research

High expression of CD28 enhanced the anti-cancer effect of siRNA-PD-1 through prompting the immune response of melanoma-bearing mice
Int Immunopharmacol. 2022 Jan 27;105:108572. doi: 10.1016/j.intimp.2022.108572. Online ahead of print.ABSTRACTImmune checkpoint blockade is considered to be an effective method of tumor immunotherapy. As one of the main immune checkpoints, blocking PD-1/PD-L1 pathway has been proved to be effective in the treatment of many cancers via activating T cells; however, many patients still do not respond to the blocking PD-1/PD-L1 treatment with satisfying results. Related research demonstrated that the activation of T cells required a co-stimulatory signal generated by the interaction between CD28 and CD80/CD86, whereas in many ...
Source: International Immunopharmacology - January 30, 2022 Category: Allergy & Immunology Authors: Huijie Jia Jing Guo Zhiang Liu Pengfei Chen Yang Li Ruipeng Li Yunfan Yang Xinyi Li Pengkun Wei Jiateng Zhong Feng Ren Mingyong Wang Jianzhuang Ren Zhiwei Feng Tiesuo Zhao Source Type: research

Effects of tolerogenic dendritic cells generated by siRNA-mediated RelB silencing on immune defense and surveillance functions of T cells.
This study demonstrates that RelB-silenced DCs could induce donor-specific hyporesponsiveness and slightly impair immune surveillance of T cells, while retaining their immune defense functions. PMID: 23665206 [PubMed - as supplied by publisher]
Source: Cellular Immunology - April 4, 2013 Category: Allergy & Immunology Authors: Luo L, Sun Z, Fang Q, Huang S, Bai X, Luo G Tags: Cell Immunol Source Type: research

IL‐1β Increases Expression of Tryptophan 2,3‐dioxygenase and Stimulates Tryptophan Catabolism in Endometrioma Stromal Cells
ConclusionIL‐1β is suggested to stimulate tryptophan catabolism and production of IL‐6 and IL‐8 by increasing TDO expression in endometriosis.
Source: American Journal of Reproductive Immunology - June 26, 2014 Category: Allergy & Immunology Authors: Yoko Urata, Kaori Koga, Yasushi Hirota, Ikumi Akiyama, Gentaro Izumi, Masashi Takamura, Miwako Nagai, Miyuki Harada, Tetsuya Hirata, Osamu Yoshino, Kei Kawana, Tomoyuki Fujii, Yutaka Osuga Tags: Original Article Source Type: research

PTEN–Foxo1 signaling triggers HMGB1-mediated innate immune responses in acute lung injury
This study was designed to investigate the role of PTEN/Foxo1 signaling in the regulation of in vivo and in vitro innate immune responses in ALI. Using a mouse model of ALI, wild-type (WT) and myeloid-specific PTEN knockout (PTENM-KO) mice were instilled with a recombinant HMGB1 (rHMGB1) or PBS. In some experiments, Foxo1 siRNA or non-specific siRNA was injected into mice 6 h prior to rHMGB1 instillation into lung. We found that rHMGB1 treatment in WT mice increased the expression of PTEN, Foxo1, TLR4, and NF-κB in alveolar macrophages from WT mice. However, macrophage-specific PTEN ablation resulted in reduced Foxo1 and...
Source: Immunologic Research - March 11, 2015 Category: Allergy & Immunology Source Type: research

Negative feedback circuit for toll like receptor-8 activation in human embryonic Kidney 293 using outer membrane vesicle delivered bi-specific siRNA
Background: TLR8 assists in antiviral approach by producing Type 1 INF via MyD88 dependent IRF7 pathway. However, over expression of INFα/β molecule poses threat by developing tolerance in chronic infection cases and enhancing inflammatory response. Here we report a bi-specific siRNA based complex which differentially activates and silences the TLR8 and MYD88 respectively in a negatively regulated fashion. Results: Outer membrane vesicle from Escherichia coli used for siRNA delivery was observed more efficient when attached with invasive protein Ail along with OmpA (P 
Source: BMC Immunology - July 23, 2015 Category: Allergy & Immunology Authors: Anurag AdhikariBirendra GuptaKrishna ManandharShravan MishraHari SaijuRajendra ShresthaNawneet MishraShishir Sharma Source Type: research