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Specialty: Cytology

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Total 80202 results found since Jan 2013.

Targeted delivery to macrophages and dendritic cells by chemically modified mannose ligand-conjugated siRNA
Nucleic Acids Res. 2022 May 7:gkac308. doi: 10.1093/nar/gkac308. Online ahead of print.ABSTRACTExtrahepatic delivery of small interfering RNAs (siRNAs) may have applications in the development of novel therapeutic approaches. However, reports on such approaches are limited, and the scarcity of reports concerning the systemically targeted delivery of siRNAs with effective gene silencing activity presents a challenge. We herein report for the first time the targeted delivery of CD206-targetable chemically modified mannose-siRNA (CMM-siRNA) conjugates to macrophages and dendritic cells (DCs). CMM-siRNA exhibited a strong bind...
Source: Cell Research - May 7, 2022 Category: Cytology Authors: Keiji Uehara Toshimasa Harumoto Asana Makino Yasuo Koda Junko Iwano Yasuhiro Suzuki Mari Tanigawa Hiroto Iwai Kana Asano Kana Kurihara Akinori Hamaguchi Hiroshi Kodaira Toshiyuki Atsumi Yoji Yamada Kazuma Tomizuka Source Type: research

Liver cancer cells as the model for developing liver-targeted RNAi therapeutics
Biochem Biophys Res Commun. 2023 Jan 6;644:85-94. doi: 10.1016/j.bbrc.2023.01.007. Online ahead of print.ABSTRACTRNAi is a sequence-specific gene regulation mechanism that involves small interfering RNAs (siRNAs). RNAi therapeutic has become a new class of precision medicine and has shown great potential in treating liver-associated diseases, especially metabolic diseases. To facilitate the development of liver-targeted RNAi therapeutics in cell model, we surveyed a panel of liver cancer cell lines for the expression of genes implicated in RNAi therapeutics including the asialoglycoprotein receptor (ASGR) and metabolic dis...
Source: Cell Research - January 14, 2023 Category: Cytology Authors: Beibei Hou Linhui Qin Linfeng Huang Source Type: research

< b > < i > In Vitro < /i > < /b > Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line
Conclusion: Our findings indicate that HAS-2 gene silencing may inhibit proliferation and promote apoptosis in the MCF-7 human breast cancer cell line.Cell Physiol Biochem 2016;40:807-817
Source: Cellular Physiology and Biochemistry - December 2, 2016 Category: Cytology Source Type: research

Inhibition of HIV replication through siRNA carried by CXCR4-targeted chimeric nanobody.
Abstract Small interfering RNA (siRNA) application in therapy still faces a major challenge with the lack of an efficient and specific delivery system. Current vehicles are often responsible for poor efficacy, safety concerns, and burden costs of siRNA-based therapeutics. Here, we describe a novel strategy for targeted delivery of siRNA molecules to inhibit human immunodeficiency virus (HIV) infection. Specific membrane translocation of siRNA inhibitor was addressed by an engineered nanobody targeting the HIV co-receptor CXCR4 (NbCXCR4) in fusion with a single-chain variable fragment (4M5.3) that carried the FITC-...
Source: Cellular and Molecular Life Sciences : CMLS - October 21, 2019 Category: Cytology Authors: Cunha-Santos C, Perdigao PRL, Martin F, Oliveira JG, Cardoso M, Manuel A, Taveira N, Goncalves J Tags: Cell Mol Life Sci Source Type: research

Combination Cancer Immunotherapy with Dendritic Cell Vaccine and Nanoparticles Loaded with Interleukin-15 and Anti-beta-catenin siRNA Significantly Inhibits Cancer Growth and Induces Anti-Tumor Immune Response
CONCLUSIONS: These findings suggest that the use of new nanocarriers such as SPION-C-CMD-FA could be an effective way to use as a novel combination therapy consisting of β-catenin siRNA, IL-15, and DC vaccine to treat cancer.PMID:35166995 | DOI:10.1007/s11095-022-03169-2
Source: Cell Research - February 15, 2022 Category: Cytology Authors: Armin Mahmoud Salehi Kheshti Farnaz Hajizadeh Asal Barshidi Bentolhoda Rashidi Farbod Ebrahimi Simin Bahmanpour Vahid Karpisheh Fatemeh Karimian Noukabadi Fariba Karoon Kiani Hadi Hassannia Fatemeh Atyabi Seyed Hossein Kiaie Fatah Kashanchi Jamshid Gholiz Source Type: research

Inflammatory gene silencing in activated monocytes by a cholesterol tagged-miRNA/siRNA: a novel approach to ameliorate diabetes induced inflammation
Cell Tissue Res. 2022 May 23. doi: 10.1007/s00441-022-03637-6. Online ahead of print.ABSTRACTThere is a major unmet need for the development of effective therapies for diabetes induced inflammation. Increased adenosine-uridine rich elements (AREs) containing mRNAs of inflammatory molecules are reported in inflamed monocytes. Destabilizing these inflammatory mRNAs by the miR-16 could reduce inflammation. DNA microarrays and in vitro cell studies showed that exogenous miR16 and its mimic treatment, in LPS/PMA induced monocytes, significantly downregulated several ARE containing inflammatory cytokine mRNAs similar to those se...
Source: Cell Research - May 23, 2022 Category: Cytology Authors: Arun Sundaramoorthy Doulathunnisa Jafar Ali Narkunaraja Shanmugam Source Type: research

Optimization of Lung Surfactant Coating of siRNA Polyplexes for Pulmonary Delivery
CONCLUSION: The addition of a coating with pulmonary surfactant to polymeric cationic polyplexes represents a valuable formulation strategy to improve local delivery of siRNA to the lungs.PMID:36447020 | DOI:10.1007/s11095-022-03443-3
Source: Cell Research - November 29, 2022 Category: Cytology Authors: Domizia Baldassi Thi My Hanh Ngo Olivia M Merkel Source Type: research

Maspin suppresses cell invasion and migration in gastric cancer through inhibiting EMT and angiogenesis via ITGB1/FAK pathway
This study aims to investigate how Maspin affects the EMT and angiogenesis of gastric cancer (GC) cells via ITGB1/FAK pathway. Immunohistochemistry was used to evaluate the expressions of Maspin, ITGB1, FAK, E-cadherin, Vimentin, D2-40, and CD34 in GC and adjacent normal tissues from 160 patients. Then, the human GC cells with different degree of differentiation were transfected with Maspin CRISPR activation plasmid, ITGB1 siRNA and/or Maspin siRNA, followed by the following experiments, including qRT-PCR, western blotting, tube formation assay, Transwell assay and wound healing. GC tumor tissues manifested decreased Maspi...
Source: Human Cell - May 13, 2020 Category: Cytology Source Type: research

CAV-1 Overexpression Exacerbates the Manifestation in EPAC-1-Induced Chronic Postsurgical Pain in Rats
CONCLUSION: CAV-1 mediates the functional coupling of microglia, astrocytes, and neurons, and thus EPAC-1/CAV-1 plays an important role in CPSP exacerbation.PMID:35958678 | PMC:PMC9357801 | DOI:10.1155/2022/8566840
Source: Cell Research - August 12, 2022 Category: Cytology Authors: Qian Hua Shiren Shen Yibin Qin Su Cao Source Type: research

Influence of suppression of CapG gene expression by siRNA on the growth and metastasis of human prostate cancer cells.
This study investigated CapG gene expression in prostate cancer cell lines; in addition, we explored the effects of CapG suppression on DU145 cell growth, and the underlying mechanism with which CapG affects DU145 cell growth and invasiveness. The expression of CapG and 18 related genes in DU145 cells was analyzed by flow cytometry, quantitative polymerase chain reaction (qPCR), CCK8 assay, western blot, and the trans-well assay. DU145 cells were transfected with designed small interfering RNA (siRNA). CapG expression was quantified by qPCR and western blot. DU145 cell proliferation and invasiveness was analyzed using the ...
Source: Cell Research - December 7, 2015 Category: Cytology Authors: Li BK, Guo K, Li CY, Li HL, Zhao PP, Chen K, Liu CX Tags: Genet Mol Res Source Type: research

Covalent conjugates of granulin-epithelial precursor-siRNA with arginine-rich peptide for improved stability and intracellular delivery in hepatoma cells
Conclusion: These results provide insights into the importance of chemical modification of unstable genetic therapeutics for HCC treatment. Conjugation of PEGylated GEP siRNA with ARP may be a potential HCC therapeutic agent.
Source: Molecular and Cellular Toxicology - June 27, 2019 Category: Cytology Source Type: research

Gene expression profile analysis of SUDHL6 cells with siRNA-mediated BCL11 A downregulation.
Abstract Our previous study has shown that downregulation of B-cell chronic lymphocytic leukemia (CLL)/lymphoma11A (BCL11A) gene by small interfering RNA (siRNA) results in the growth inhibition and apoptosis of B cell lymphoma cell line SUDHL6. To gain further insight into the molecular mechanisms of this process and identify the differentially expressed genes in SUDHL6 cells after BCL11A downregulation, the global gene expression profile was identified and analyzed using the Affymetrix HG-U133 Plus 2.0 array.Twenty-one differentially expressed genes were validated and analyzed from the BCL11 A siRNA-treated SUDH...
Source: Cell Biology International - July 7, 2014 Category: Cytology Authors: Wu H, Li Y Tags: Cell Biol Int Source Type: research

β-Adducin siRNA disruption of the spectrin-based cytoskeleton in differentiating keratinocytes prevented by calcium acting through calmodulin/epidermal growth factor receptor/cadherin pathway.
Abstract Here, we report that siRNA transfection of β-adducin significantly disrupted the spectrin-based cytoskeleton and cytoskeletal arrangements of both β-adducin and PKCδ by substantially inhibiting the expression of β-adducin, spectrin and PKCδ proteins in differentiating keratinocytes. However, extracellular Ca(2+) treatment blocked the inhibitory effects of the β-adducin siRNA. Ca(2+) also prevented the significant down-regulation of two differentiation markers involucrin and K1/10 and the distinct up-regulation of proliferation marker K14 in β-adducin siRNA transfected keratinocytes. In addition, β...
Source: Cellular Signalling - October 7, 2014 Category: Cytology Authors: Wu J, Masci PP, Chen C, Chen J, Lavin MF, Zhao KN Tags: Cell Signal Source Type: research

Silencing of MUC8 by siRNA increases P2Y2-induced airway inflammation.
Abstract Mucin hypersecretion and overproduction are frequent manifestations of respiratory disease. Determining the physiological function of airway mucin is presently considered more important than identifying the relevant signaling pathways. The lack of a full-length human MUC8 cDNA sequence has hindered the generation of a Muc8 knockout mouse line. Thus, the precise physiological functions of MUC8 are unclear. Herein, we investigated the function of MUC8 using a siRNA-mediated genetic silencing approach in human airway epithelial cells. Herein, intracellular IL-1α production was stimulated by an ATP/P2Y2 comp...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - January 9, 2015 Category: Cytology Authors: Cha HJ, Jung MS, Ahn DW, Choi JK, Ock MS, Kim KS, Yoon JH, Song EJ, Song KS Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

TLR4 Influences Hepatitis B Virus Related Hepatocellular Carcinoma by Regulating the Wnt/ β-Catenin Pathway
Conclusions: TLR4 can affect the expression of β-catenin and hence influence the progression of HBV-related HCC.Cell Physiol Biochem 2017;42:469 –479
Source: Cellular Physiology and Biochemistry - June 2, 2017 Category: Cytology Source Type: research