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Total 61743 results found since Jan 2013.

Doxorubicin induced ROS-dependent HIF1 α activation mediates blockage of IGF1R survival signaling by IGFBP3 promotes cardiac apoptosis
In this study, we investigated the role of IGFBP3, HIF1A, and PHD in Dox-induced cardiac apoptosis. Cells challenged with 1 μM Dox for 24 h increased ROS generation, augmented intracellular and secreted IGFBP3 levels, and reduced IGF1 signaling. Further, we showed that Dox enhanced the extracellular association of IGF1 with IGFBP3. Moreover, echocardiography parameters, especially ejection fraction (EF) and fractional shortening (FS) were significantly reduced in ventricle tissue of Dox challenged rats. Notably, siRNA approach against IGFBP3 or an anti-IGFBP3 antibody rescued Dox-induced cardiac apoptosis, mitochondrial R...
Source: Aging - January 5, 2023 Category: Biomedical Science Authors: Su-Ying Wen Ayaz Ali I-Chieh Huang Jian-Sheng Liu Po-Yuan Chen Vijaya Padma Viswanadha Chih-Yang Huang Wei-Wen Kuo Source Type: research

Budding uninhibited by benzimidazoles-1 (BUB1) regulates EGFR signaling by reducing EGFR internalization
Aging (Albany NY). 2023 Jul 3;15. doi: 10.18632/aging.204820. Online ahead of print.ABSTRACTEGFR signaling initiates upon ligand binding which leads to activation and internalization of the receptor-ligand complex. Here, we evaluated if BUB1 impacted EGFR signaling by regulating EGFR receptor internalization and activation. BUB1 was ablated genomically (siRNA) or biochemically (2OH-BNPP1) in cells. EGF ligand was used to initiate EGFR signaling while disuccinimidyl suberate (DSS) was used for cross linking cellular proteins. EGFR signaling was measured by western immunoblotting and receptor internalization was evaluated by...
Source: Aging - July 3, 2023 Category: Biomedical Science Authors: Shyam Nyati Grant Young Corey Speers Mukesh K Nyati Alnawaz Rehemtulla Source Type: research

Hypoxia activates muscle-restricted coiled-coil protein (MURC) expression via transforming growth factor-{beta} in cardiac myocytes
In conclusion, hypoxia in cultured rat neonatal cardiomyocytes increased MURC expression via the induction of TGF-β, SRF and the ERK pathway. These findings suggest that MURC plays a role in hypoxia-induced hypertrophy in cardiomyocytes.
Source: Clinical Science - September 4, 2013 Category: Biomedical Science Authors: K Shyu, W Cheng, B Wang, H Chang Source Type: research

Death-associated protein kinase3 mediates vascular structural remodeling via stimulating smooth muscle cell proliferation and migration
Death-associated protein kinase (DAPK)3 also known as zipper-interacting kinase is a serine/threonine kinase that mainly regulates cell death and smooth muscle contraction. We previously found that protein expression of DAPK3 increases in mesenteric artery from spontaneously hypertensive rats (SHR) and that DAPK3 mediates the development of hypertension in SHR partly through promoting reactive oxygen species-dependent vascular inflammation. However, it remains to be clarified how DAPK3 controls smooth muscle cells (SMCs) proliferation and migration that are also important processes for the hypertension development. We ther...
Source: Clinical Science - May 12, 2014 Category: Biomedical Science Authors: T Usui, T Sakatsume, R Nijima, K Otani, K Kazama, T Morita, S Kameshima, M Okada, H Yamawaki Source Type: research

Role of (pro)renin receptor in Ang II-mediated EGF receptor transactivation.
Authors: Shibayama Y, Hitomi H, Nakano D, Kobori H, Mori H, Deguchi K, Masaki T, Ichihara A, Nishiyama A Abstract Prorenin-induced intracellular signaling pathway is not fully elucidated. We investigated whether the (pro)renin receptor mediates epidermal growth factor (EGF) receptor transactivation through angiotensin (Ang) II-dependent and -independent pathways in human embryo kidney 293 cells. Prorenin (2 nmol/L) caused biphasic phosphorylation of EGF receptor (Tyr992) and extracellular signal-regulated kinase (ERK) 1/2, peaking at 5 minutes followed by a decrease and a second peak at 60-120 minutes, whereas EGF ...
Source: Frontiers in Bioscience - Elite - November 25, 2014 Category: Biomedical Science Tags: Front Biosci (Elite Ed) Source Type: research

Molecular regulation of the expression of leptin by hypoxia in human coronary artery smooth muscle cells
Conclusion: Hypoxia in HCASMCs increases leptin expression through the induction of AngII, ROS, and the JNK pathway to enhance atherosclerosis in HCASMCs.
Source: Journal of Biomedical Science - January 9, 2015 Category: Biomedical Science Authors: Chiung-Zuan ChiuBao-Wei WangKou-Gi Shyu Source Type: research

Inhibitory Mechanism of FAT4 Gene Expression in Response to Actin Dynamics during Src-Induced Carcinogenesis
In this study, we show that transient activation of the Src oncoprotein represses FAT4 mRNA expression through actin depolymerization in the immortalized normal human mammary epithelial cell line MCF-10A. Src activation causes actin depolymerization via the MEK/Erk/Cofilin cascade. The MEK inhibitor U0126 blocks the inhibitory effect of Src on FAT4 mRNA expression and Src-induced actin depolymerization. To determine whether actin dynamics act on the regulation of FAT4 mRNA expression, we treated MCF-10A cells with the ROCK inhibitor Y-27632. Y-27632 treatment decreased FAT4 mRNA expression. This suppressive effect was bloc...
Source: PLoS One - February 13, 2015 Category: Biomedical Science Authors: Takao Ito et al. Source Type: research

Polyphosphate-induced matrix metalloproteinase-3-mediated proliferation in rat dental pulp fibroblast-like cells is mediated by a Wnt5 signaling cascade.
Authors: Ozeki N, Yamaguchi H, Hase N, Hiyama T, Kawai R, Kondo A, Nakata K, Mogi M Abstract Although it is known that inorganic polyphosphate [Poly(P)] induces differentiation of osteoblasts, there are few reports concerning its effects on cell proliferation, especially in fibroblasts. Because we found that Poly(P) stimulates the proliferation of purified rat dental pulp fibroblast-like cells (DPFCs), matrix metalloproteinase (MMP)-3 small interfering RNA (siRNA) was transfected into purified rat DPFCs to investigate whether MMP-3 activity is induced by Poly(P) and/or is associated with cell proliferation in DPFCs...
Source: BioScience Trends - July 16, 2015 Category: Biomedical Science Tags: Biosci Trends Source Type: research

Transcriptional Factor Snail Mediates Epithelial-Mesenchymal Transition in Human Bronchial Epithelial Cells Induced by Silica.
Abstract Epithelial-mesenchymal transition (EMT) plays an important role in fibrotic diseases. We have previously showed that silica induces EMT in human bronchial epithelial cells (BECs); however, the underlying mechanism of silica-induced EMT is poorly understood. In the present study, we investigated the role of Snail in silica-induced EMT in human BECs in vitro. Human BECs were treated with silica at various concentrations and incubation times. Then MTT assay, western blot, electrophoretic mobility shift assay (EMSA), and small interfering RNA (siRNA) transfection were performed. We found that silica increased...
Source: Biomedical and Environmental Sciences : BES - July 1, 2015 Category: Biomedical Science Authors: Hu YB, Li FF, Deng ZH, Pan PH Tags: Biomed Environ Sci Source Type: research

Dexmedetomidine-Induced Contraction in the Isolated Endothelium-Denuded Rat Aorta Involves PKC-δ-mediated JNK Phosphorylation.
Authors: Yu J, Ok SH, Kim WH, Cho H, Park J, Shin IW, Lee HK, Chung YK, Choi MJ, Kwon SC, Sohn JT Abstract Vasoconstriction mediated by the highly selective alpha-2 adrenoceptor agonist dexmedetomidine leads to transiently increased blood pressure and severe hypertension. The dexmedetomidine-induced contraction involves the protein kinase C (PKC)-mediated pathway. However, the main PKC isoform involved in the dexmedetomidine-induced contraction remains unknown. The goal of this in vitro study was to examine the specific PKC isoform that contributes to the dexmedetomidine-induced contraction in the isolated rat aort...
Source: International Journal of Medical Sciences - September 25, 2015 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Transcriptional Factor Snail Mediates Epithelial-Mesenchymal Transition in Human Bronchial Epithelial Cells Induced by Silica
Publication date: July 2015 Source:Biomedical and Environmental Sciences, Volume 28, Issue 7 Author(s): Yong Bin HU, Fei Feng LI, Zheng Hao DENG, Pin Hua PAN Epithelial-mesenchymal transition (EMT) plays an important role in fibrotic diseases. We have previously showed that silica induces EMT in human bronchial epithelial cells (BECs); however, the underlying mechanism of silica-induced EMT is poorly understood. In the present study, we investigated the role of Snail in silica-induced EMT in human BECs in vitro. Human BECs were treated with silica at various concentrations and incubation times. Then MTT assay, wester...
Source: Biomedical and Environmental Sciences - November 11, 2015 Category: Biomedical Science Source Type: research

Identification of Pol IV and RDR2-dependent precursors of 24 nt siRNAs guiding de novo DNA methylation in Arabidopsis
In Arabidopsis thaliana, abundant 24 nucleotide small interfering RNAs (24 nt siRNA) guide the cytosine methylation and silencing of transposons and a subset of genes. 24 nt siRNA biogenesis requires nuclear RNA polymerase IV (Pol IV), RNA-dependent RNA polymerase 2 (RDR2) and DICER-like 3 (DCL3). However, siRNA precursors are mostly undefined. We identified Pol IV and RDR2-dependent RNAs (P4R2 RNAs) that accumulate in dcl3 mutants and are diced into 24 nt RNAs by DCL3 in vitro. P4R2 RNAs are mostly 26-45 nt and initiate with a purine adjacent to a pyrimidine, characteristics shared by Pol IV transcripts generated in vitro...
Source: eLife - December 8, 2015 Category: Biomedical Science Authors: Blevins, T., Podicheti, R., Mishra, V., Marasco, M., Wang, J., Rusch, D., Tang, H., Pikaard, C. S. Tags: Genes and chromosomes Source Type: research

Multiple Sites of Type II Site Ligand (Luteolin and BMHPC) Regulation of Gene Expression in PC-3 Cells.
Authors: Markaverich BM, Vijjeswarapu M Abstract Type II [(3)H]estradiol binding site ligands including luteolin (a naturally occurring bioflavonoid) and synthetic compounds such as 2,6-bis((3-methoxy-4-hydroxyphenyl)methylene)cyclohexanone (BMHPC) inhibit normal and malignant prostate cell (PC-3, LNCaP, DU-145) proliferation in vitro and in vivo. Type II sites represent a binding domain on histone H4 possibly involved in an epigenetic mechanism for controlling gene transcription. Treatment of PC-3 human prostate cancer cells with luteolin or BMHPC modulated the expression of a number of genes in the epidermal grow...
Source: International Journal of Biomedical Science - December 12, 2015 Category: Biomedical Science Tags: Int J Biomed Sci Source Type: research

Ampelopsin Improves Insulin Resistance by Activating PPARγ and Subsequently Up-Regulating FGF21-AMPK Signaling Pathway
by Yong Zhou, Ying Wu, Yu Qin, Lei Liu, Jing Wan, Lingyun Zou, Qianyong Zhang, Jundong Zhu, Mantian Mi Ampelopsin (APL), a major bioactive constituent of Ampelopsis grossedentata, exerts a number of biological effects. Here, we explored the anti-diabetic activity of APL and elucidate the underlying mechanism of this action. In palmitate-induced insulin resistance of L6 myotubes, APL treatment markedly up- regulated phosphorylated insulin receptor substrate-1 and protein kinase B, along with a corresponding increase of glucose uptake capacity. APL treatment also increased expressions of fibroblast growth factor (FGF21) and...
Source: PLoS One - July 7, 2016 Category: Biomedical Science Authors: Yong Zhou Source Type: research

Yangjing Capsule extract promotes proliferation of GC-1 spg cells via up-regulated POU3F1 pathway.
The objective of this study is to determine whether the transcription factor POU3F1 is up-regulated by YC extract through the PI3K/AKT signaling pathway to regulate SSCs survival and proliferation. Cultured GC-1 spermatogonial (spg) cells were treated with 0.01, 0.1, and 1 mg/mL YC extract for 48 h. Cell viability was analyzed using MTT assay, while POU3F1 expression was quantitatively detected using real time-polymerase chain reaction and Western blot analysis. POU3F1, GDNF family receptor alpha1 (GFRα1) short interfering ribonucleic acid (siRNA), and LY294002 (PI3K inhibitor) were applied as blockers to explore the unde...
Source: BioScience Trends - February 7, 2017 Category: Biomedical Science Tags: Biosci Trends Source Type: research