Autism and associated disorders: cannabis as a potential therapy
Front Biosci (Elite Ed). 2022 Jan 13;14(1):1. doi: 10.31083/j.fbe1401001.ABSTRACTAutism spectrum disorder (ASD) is a group of disabilities with impairments in physical, verbal, and behavior areas. Regardless the growing frequency of autism, no medicine has been formed for the management of the ASD primary symptoms. The most frequently prescribed drugs are off-label. Therefore, there is necessity for an advance tactic for the treatment of autism. The endocannabinoid system has a central role in ruling emotion and social behaviors. Dysfunctions of the system donate to the behavioral deficits in autism. Therefore, the endocan...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Mariana Babayeva Haregewein Assefa Paramita Basu Zvi Loewy Source Type: research

Clinical grade adjuvants to mature CD141 < sup > + < /sup > DCs for immunotherapy
Front Biosci (Elite Ed). 2022 Jan 20;14(1):2. doi: 10.31083/j.fbe1401002.ABSTRACTStimulation of dendritic cells (DC) is considered critical in cancer immunotherapy. BATF-3-dependent subsets, that express in humans CD141 (BDCA-3), promote CD8 T-cell cross-priming against tumor antigens. Here, we evaluate two clinical-grade stimuli for peripheral blood CD141+ myeloid dendritic cells (mDCs), a rare DC subset that is currently being explored for use in immunotherapy. In contrast to routine evaluation methods, which focus on predefined maturation markers on the surface or factors released from the activated cells, we applied an...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Blanca Alegr ía Carlos Alfaro Source Type: research

Effect of salicylic acid on phenolic compounds, antioxidant and antihyperglycemic activity of < em > Lamiaceae < /em > plants grown in a temperate climate
CONCLUSIONS: The results obtained on the stimulating effects of salicylic acid can be used to increase the nutritional and pharmacological value of plants of the Lamiaceae family cultivated in temperate climates.PMID:35320907 | DOI:10.31083/j.fbe1401003 (Source: Frontiers in Bioscience - Elite)
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Liubov Skrypnik Anton Golovin Tamara Savina Source Type: research

Nutritional status in the pediatric oncology patients
Front Biosci (Elite Ed). 2022 Feb 8;14(1):4. doi: 10.31083/j.fbe1401004.ABSTRACTNutritional status plays a vital role in the growth of children. In pediatric patients, disease-related malnutrition is a dynamic and multifactorial process supported by several factors such as inflammation, increased energy expenditure, decreased intake or reduced utilization of nutrients. In pediatric patients with malignancies, sarcopenia may coexist with malnutrition, amplifying its negative impact on prognosis. Careful monitoring of nutritional status both at diagnosis and during chemotherapy treatment allows early detection of the risk an...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Silvia Triarico Emanuele Rinninella Giorgio Attin à Alberto Romano Palma Maurizi Stefano Mastrangelo Antonio Ruggiero Source Type: research

Applicability of water from the Bay of Gda ńsk as a growth medium for mixotrophic culture of < em > Platymonas subcordiformis < /em >
This study aimed to determine the efficiency of P. subcordiformis biomass production in a medium prepared with water from the Bay of Gdańsk. The tested medium supported high biomass growth rates which reached 317.58 ± 42.31 mgV⁢S/dm3⋅d in the best-performing variant, with a final concentration of 3493.3 ± 465.4 mgV⁢S/dm3⋅d. In the autotrophic culture, nitrogen and phosphorus removal exceeded 98%. Amending the mixotrophic culture with glucose did not affect P. subcordiformis concentrations. However, it did significantly limit the demand for nutrients in the biomass and reduced chlorophyll a production in the cell...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Magda Dudek Marcin D ębowski Anna Nowicka Joanna Kazimierowicz Marcin Zieli ński Source Type: research

< em > In vitro < /em > study of L-asparaginase enzyme activity by two yeast strains on food matrixes and the relative effect on fungal pathogens growth
Front Biosci (Elite Ed). 2022 Feb 14;14(1):6. doi: 10.31083/j.fbe1401006.ABSTRACTAsparagine is one of the precursors of acrylamide and toxic fungal secondary metabolites, both carcinogenic compounds. In the present study, the optimal conditions to deplete asparagine by Aureobasidium pullulans (L1 and L8) from potato and wheat flour matrices were investigated. Through a colorimetric plate-assay with phenol red as indicator dye, both strains demonstrated to be able to produce L-asparaginase from 20 ∘C to 30 ∘C for L1 and only at 20 ∘C for L8 strain starting from 48 h of incubation. The ability of both yeasts to reduce ...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Alessandra Di Francesco Source Type: research

Polyclonal antibodies against potato spindle tuber viroid RNA
Front Biosci (Elite Ed). 2022 Mar 4;14(1):7. doi: 10.31083/j.fbe1401007.ABSTRACTRibonucleic acid (RNA) can act as a hapten in the direct immunization of animals. For antigen synthesis, 65 mg of viroid RNA were obtained by in vitro transcription of the recombinant DNA. We received a reasonable immune response in mice and rabbits with synthesized conjugate viroid RNA-lysozyme. Analyses of polyclonal mouse and rabbit antisera as well as estimates of antibody specificity were performed by dot-Enzyme Linked Immunosorbent Assay (ELISA), sandwich ELISA, and northern immunoblotting. Antiserum obtained showed strong cross-reactions...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Yuri Drygin Tatiana Gasanova Konstantin Butenko Source Type: research

Autism and associated disorders: cannabis as a potential therapy
Front Biosci (Elite Ed). 2022 Jan 13;14(1):1. doi: 10.31083/j.fbe1401001.ABSTRACTAutism spectrum disorder (ASD) is a group of disabilities with impairments in physical, verbal, and behavior areas. Regardless the growing frequency of autism, no medicine has been formed for the management of the ASD primary symptoms. The most frequently prescribed drugs are off-label. Therefore, there is necessity for an advance tactic for the treatment of autism. The endocannabinoid system has a central role in ruling emotion and social behaviors. Dysfunctions of the system donate to the behavioral deficits in autism. Therefore, the endocan...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Mariana Babayeva Haregewein Assefa Paramita Basu Zvi Loewy Source Type: research

Clinical grade adjuvants to mature CD141 < sup > + < /sup > DCs for immunotherapy
Front Biosci (Elite Ed). 2022 Jan 20;14(1):2. doi: 10.31083/j.fbe1401002.ABSTRACTStimulation of dendritic cells (DC) is considered critical in cancer immunotherapy. BATF-3-dependent subsets, that express in humans CD141 (BDCA-3), promote CD8 T-cell cross-priming against tumor antigens. Here, we evaluate two clinical-grade stimuli for peripheral blood CD141+ myeloid dendritic cells (mDCs), a rare DC subset that is currently being explored for use in immunotherapy. In contrast to routine evaluation methods, which focus on predefined maturation markers on the surface or factors released from the activated cells, we applied an...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Blanca Alegr ía Carlos Alfaro Source Type: research

Effect of salicylic acid on phenolic compounds, antioxidant and antihyperglycemic activity of < em > Lamiaceae < /em > plants grown in a temperate climate
CONCLUSIONS: The results obtained on the stimulating effects of salicylic acid can be used to increase the nutritional and pharmacological value of plants of the Lamiaceae family cultivated in temperate climates.PMID:35320907 | DOI:10.31083/j.fbe1401003 (Source: Frontiers in Bioscience - Elite)
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Liubov Skrypnik Anton Golovin Tamara Savina Source Type: research

Nutritional status in the pediatric oncology patients
Front Biosci (Elite Ed). 2022 Feb 8;14(1):4. doi: 10.31083/j.fbe1401004.ABSTRACTNutritional status plays a vital role in the growth of children. In pediatric patients, disease-related malnutrition is a dynamic and multifactorial process supported by several factors such as inflammation, increased energy expenditure, decreased intake or reduced utilization of nutrients. In pediatric patients with malignancies, sarcopenia may coexist with malnutrition, amplifying its negative impact on prognosis. Careful monitoring of nutritional status both at diagnosis and during chemotherapy treatment allows early detection of the risk an...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Silvia Triarico Emanuele Rinninella Giorgio Attin à Alberto Romano Palma Maurizi Stefano Mastrangelo Antonio Ruggiero Source Type: research

Applicability of water from the Bay of Gda ńsk as a growth medium for mixotrophic culture of < em > Platymonas subcordiformis < /em >
This study aimed to determine the efficiency of P. subcordiformis biomass production in a medium prepared with water from the Bay of Gdańsk. The tested medium supported high biomass growth rates which reached 317.58 ± 42.31 mgV⁢S/dm3⋅d in the best-performing variant, with a final concentration of 3493.3 ± 465.4 mgV⁢S/dm3⋅d. In the autotrophic culture, nitrogen and phosphorus removal exceeded 98%. Amending the mixotrophic culture with glucose did not affect P. subcordiformis concentrations. However, it did significantly limit the demand for nutrients in the biomass and reduced chlorophyll a production in the cell...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Magda Dudek Marcin D ębowski Anna Nowicka Joanna Kazimierowicz Marcin Zieli ński Source Type: research

< em > In vitro < /em > study of L-asparaginase enzyme activity by two yeast strains on food matrixes and the relative effect on fungal pathogens growth
Front Biosci (Elite Ed). 2022 Feb 14;14(1):6. doi: 10.31083/j.fbe1401006.ABSTRACTAsparagine is one of the precursors of acrylamide and toxic fungal secondary metabolites, both carcinogenic compounds. In the present study, the optimal conditions to deplete asparagine by Aureobasidium pullulans (L1 and L8) from potato and wheat flour matrices were investigated. Through a colorimetric plate-assay with phenol red as indicator dye, both strains demonstrated to be able to produce L-asparaginase from 20 ∘C to 30 ∘C for L1 and only at 20 ∘C for L8 strain starting from 48 h of incubation. The ability of both yeasts to reduce ...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Alessandra Di Francesco Source Type: research

Polyclonal antibodies against potato spindle tuber viroid RNA
Front Biosci (Elite Ed). 2022 Mar 4;14(1):7. doi: 10.31083/j.fbe1401007.ABSTRACTRibonucleic acid (RNA) can act as a hapten in the direct immunization of animals. For antigen synthesis, 65 mg of viroid RNA were obtained by in vitro transcription of the recombinant DNA. We received a reasonable immune response in mice and rabbits with synthesized conjugate viroid RNA-lysozyme. Analyses of polyclonal mouse and rabbit antisera as well as estimates of antibody specificity were performed by dot-Enzyme Linked Immunosorbent Assay (ELISA), sandwich ELISA, and northern immunoblotting. Antiserum obtained showed strong cross-reactions...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Yuri Drygin Tatiana Gasanova Konstantin Butenko Source Type: research

Autism and associated disorders: cannabis as a potential therapy
Front Biosci (Elite Ed). 2022 Jan 13;14(1):1. doi: 10.31083/j.fbe1401001.ABSTRACTAutism spectrum disorder (ASD) is a group of disabilities with impairments in physical, verbal, and behavior areas. Regardless the growing frequency of autism, no medicine has been formed for the management of the ASD primary symptoms. The most frequently prescribed drugs are off-label. Therefore, there is necessity for an advance tactic for the treatment of autism. The endocannabinoid system has a central role in ruling emotion and social behaviors. Dysfunctions of the system donate to the behavioral deficits in autism. Therefore, the endocan...
Source: Frontiers in Bioscience - Elite - March 23, 2022 Category: Biomedical Science Authors: Mariana Babayeva Haregewein Assefa Paramita Basu Zvi Loewy Source Type: research