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< em > Aristophania vespae < /em > gen. nov., sp. nov., isolated from wasps, is related to < em > Bombella < /em > and < em > Oecophyllibacter < /em > , isolated from bees and ants
In this study, two new strains DDB2-T1T (=KACC 21507T=LMG 31759T) and DM15PD (=CCM 9165=DSM 112731=KACC 22353=LMG 32454) were isolated from wasps collected in the Republic of Korea and Germany, respectively. Molecular and phenotypic analysis revealed that the strains are closely related, with 16S rRNA gene sequences showing 100 % identity and genomic average nucleotide identity (ANI) values ≥99 %. The closest related species based on type strain 16S rRNA gene sequences are Swingsia samuiensis, Acetobacter peroxydans, Bombella favorum and Bombella intestini (94.8-94.7% identity), whereas the closest related species based ...
Source: International Journal of Systematic and Evolutionary Microbiology - February 7, 2023 Category: Microbiology Authors: Juan Guzman Miyoung Won Anja Poehlein Atena Sadat Sombolestani Daniela Mayorga-Ch David Laureys Jessika De Clippeleer Peter K ämpfer Rolf Daniel Andreas Vilcinskas Peter Vandamme Soon-Wo Kwon Source Type: research

Nerolidol, a sesquiterpene, attenuates oxidative stress and inflammation in acetic acid-induced colitis in rats
Mol Cell Biochem. 2021 May 17. doi: 10.1007/s11010-021-04094-5. Online ahead of print.ABSTRACTTargeting oxidative stress and inflammation by novel dietary compounds of natural origin convincingly appears to be one of the most important therapeutic strategies to keep inflammatory bowel diseases (IBD) such as ulcerative colitis disease in remission. It is imperative to investigate naturally occuring plant-derived dietary phytochemicals that are receiving attention for their therapeutic benefits to overcome the debilitating conditions of IBD. In the present study, the effect of nerolidol (NRD), a monocyclic sesquiterpene foun...
Source: Molecular and Cellular Biochemistry - May 17, 2021 Category: Biochemistry Authors: Salim M A Bastaki Naheed Amir Ernest Adeghate Shreesh Ojha Source Type: research

Detailed Dissection of UBE3A-Mediated DDI1 Ubiquitination
Discussion Poly-ubiquitinated proteins targeted for degradation might be recognized directly by proteasomal receptors or by proteasomal shuttling proteins. The first shuttling proteins – Ddi1, Rad23 and Dsk2 – were identified and characterized in Saccharomyces cerevisiae (Lambertson et al., 1999; Kaplun et al., 2005). Proteasomal shuttles contain an N-terminal ubiquitin-like (UBL) domain that interacts with the 26S proteasome (Finley, 2009), and a C-terminal ubiquitin-binding domain domain (UBD) that binds to ubiquitin or poly-ubiquitin chains (Bertolaet et al., 2001). When ubiquitinated, substrates are capt...
Source: Frontiers in Physiology - May 2, 2019 Category: Physiology Source Type: research

Crystal Structure of the Monomeric Extracellular Domain of α9 Nicotinic Receptor Subunit in Complex With α-Conotoxin RgIA: Molecular Dynamics Insights Into RgIA Binding to α9α10 Nicotinic Receptors
Conclusion The first crystal structure of a human nAChR domain with an α-Ctx is presented. The structure revealed the interactions between α-Ctx RgIA and the (+) side of neuronal nAChR α9-ECD in high detail. Based on the structure of this complex, models of human α9α10 nAChR ECD with fully formed binding sites were constructed with RgIA bound to each of them. Our MD simulations suggest that the favorable binding site of RgIA in the human α9α10 nAChR ECD consists of either α9 or α10 subunits as the (+) side and of an adjacent α9 rather than α10 su...
Source: Frontiers in Pharmacology - April 30, 2019 Category: Drugs & Pharmacology Source Type: research

Discovering, Characterizing, and Applying Acyl Homoserine Lactone-Quenching Enzymes to Mitigate Microbe-Associated Problems Under Saline Conditions
Conclusion In this study, bacterial species that proliferate under saline conditions were isolated and screened to select the ones that are positive for QQ under high salinity and high temperatures. Further genomic and biochemical characterization revealed a particularly promising AiiA from the Altererythrobacter sp. S1-5 that demonstrated a broad AHL substrate specificity range and enzymatic activity at an optimal pH of 8 and 50°C. AiiAS1-5 was also able to maintain good relative QQ activity with increasing salt concentrations up to 2 M. Its QQ efficacy against P. aeruginosa, A. hydrophila, and V. alginolyticus wa...
Source: Frontiers in Microbiology - April 16, 2019 Category: Microbiology Source Type: research

Structural and Proteomic Changes in Viable but Non-culturable Vibrio cholerae
This study gives unprecedented insight into the physiology of VBNC cells and the drastically altered presence of their metabolic and structural proteins. Introduction Changes in the physical and chemical properties of their environment, such as heat, cold and salt stress, oxygen and nutrient deprivation, desiccation and changes in osmolarity, threaten the survival of bacteria, and they have therefore evolved various strategies to evade detrimental effects (Merchant and Helmann, 2012; Alvarez-Ordóñez et al., 2015; Guan et al., 2017). The facultative human pathogen Vibrio cholerae, is common in brackish...
Source: Frontiers in Microbiology - April 16, 2019 Category: Microbiology Source Type: research

Comparative FISH-Mapping of MC1R, ASIP, and TYRP1 in New and Old World Camelids and Association Analysis With Coat Color Phenotypes in the Dromedary (Camelus dromedarius)
This study was supported by grants from Alpaca Research Foundation (ARF) 2009–2011 and Morris Animal Foundation (MAF) D09LA-004, D14LA-005. The authors highly appreciate donations to ARF and MAF by Leslie Herzog of Herzog Alpacas. FA was supported by Qassim University, Saudi Arabia. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The handling Editor and reviewer PO-tW declared their involvement as co-editors in the Research Topic, and confirm the absence of an...
Source: Frontiers in Genetics - April 15, 2019 Category: Genetics & Stem Cells Source Type: research

Lipidomics Reveals Seasonal Shifts in a Large-Bodied Hibernator, the Brown Bear
Conclusion Our study showed the interesting result that, even if the brown bear hibernates at shallow hypothermia (30–36°C), selective mobilizations and utilizations of lipids also occur, as they do in small hibernators with more pronounced Tb reduction during hibernation. Indeed, tissues appeared to preferentially retain MUFAs over PUFAs, and to mobilize SFAs for distribution and oxidation. Omega-3 fatty acids, precursors of numerous metabolic pathways, were sequestered in WAT. TGs with short-length fatty acids, prone to oxidation at a lower ATP cost, were released into the plasma, whereas those with longes...
Source: Frontiers in Physiology - April 11, 2019 Category: Physiology Source Type: research

Characterizing Relationship of Microbial Diversity and Metabolite in Sichuan Xiaoqu
Conclusion The difference in microbial community structures among different Xiaoqu samples was significant. The saccharifying- and liquefying-power were positively correlated with α- and β-diversity of bacteria but was also positively correlated with that of fungi. Staphylococcus and Weissella were dominant bacteria, while Rhizopus and Candida were dominant fungi, and abundance ranged from 33.66 to 91.53% for the former, while it was 58.51 and 48.72%, respectively in sample No3 and sample No4, for the latter. The difference of microbial community diversity resulted in the discrepancy of the volatile profile ...
Source: Frontiers in Microbiology - April 11, 2019 Category: Microbiology Source Type: research

Quantitative Proteomic Analysis of the Response of Probiotic Putative Lactococcus lactis NCDO 2118 Strain to Different Oxygen Availability Under Temperature Variation
Conclusion In this work we applied the label-free proteomics approach to quantify the L. lactis subsp. lactis NCDO 2118 proteome in response to NAE or AN conditions under different temperatures. This comparative proteomic analysis generates different proteomic profiles which enabled the validation of in silico data of the L. lactis NCDO 2118 genome. The quantitative changes detected in our proteomic analysis showed the versatility of the functional genome of NCDO 2118, which reflects in its physiological adaptation to environmental changes that might be found during its passage inside TGI or in industrial processes. Altog...
Source: Frontiers in Microbiology - April 10, 2019 Category: Microbiology Source Type: research