Structural and Proteomic Changes in Viable but Non-culturable Vibrio cholerae

This study gives unprecedented insight into the physiology of VBNC cells and the drastically altered presence of their metabolic and structural proteins. Introduction Changes in the physical and chemical properties of their environment, such as heat, cold and salt stress, oxygen and nutrient deprivation, desiccation and changes in osmolarity, threaten the survival of bacteria, and they have therefore evolved various strategies to evade detrimental effects (Merchant and Helmann, 2012; Alvarez-Ordóñez et al., 2015; Guan et al., 2017). The facultative human pathogen Vibrio cholerae, is common in brackish and estuarine waters where it is often associated with aquatic flora and fauna (Blake et al., 1977; Huq et al., 1983; Tamplin et al., 1990; Balakrish Nair et al., 1991). Like many other bacteria, V. cholerae enters a state of restrained metabolic activity when confronted with cues like low temperatures and or low nutrient availability over extended periods of time (Xu et al., 1982; Mederma et al., 1992; Rahman et al., 1994; Oliver et al., 1995; Oliver, 2005, 2010; Morishige et al., 2015; Pinto et al., 2015). Once the cells have entered this state, V. cholerae does not readily start to grow and reproduce when they are returned to more favorable and nutrient-rich media, and their status has, therefore, been termed “viable but non-culturable” (VBNC) (Xu et al., 1982; Kell et al., 1998; Bergkessel et al., 2016). Under standard laboratory conditions, ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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