Quantitative Proteomic Analysis of the Response of Probiotic Putative Lactococcus lactis NCDO 2118 Strain to Different Oxygen Availability Under Temperature Variation

Conclusion In this work we applied the label-free proteomics approach to quantify the L. lactis subsp. lactis NCDO 2118 proteome in response to NAE or AN conditions under different temperatures. This comparative proteomic analysis generates different proteomic profiles which enabled the validation of in silico data of the L. lactis NCDO 2118 genome. The quantitative changes detected in our proteomic analysis showed the versatility of the functional genome of NCDO 2118, which reflects in its physiological adaptation to environmental changes that might be found during its passage inside TGI or in industrial processes. Altogether, these findings will help to improve our understanding about the molecular basis of the physiology of L. lactis NCDO 2118 as well as the robustness of this strain in response to stress conditions. Author Contributions WM, HF, and VA conceived and designed the experiments. WM, CS, and GT performed microbiological analyses and sample preparation for proteomic analysis. WM, GT, and CR conducted the proteomic analysis. LO, SS, and FP performed bioinformatics analysis. PG, HF, and VA contributed substantially to data interpretation and revisions. WM, HF, and VA participated in all steps of the project as coordinators, and critically reviewed the manuscript. All authors read and approved the final manuscript. Funding This work was supported by the Brazilian Federal Agency for the Support and Evaluation of Graduate Education (CAPES), Minas Gerais Research...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research